Moringa Leaf Extract Research Articles

Edukasi Manfaat Pangan Lokal untuk Peningkatan Produksi ASI Ibu Menyusui di PMB Yustati Ambarita Muaro Jambi

The achievement of exclusive breastfeeding in Jambi province in 2020 reached 59.9%. This number has not met the target of exclusive breastfeeding set nationally by the government, which is 80% of the number of babies in Indonesia. One of the contributing factors is the less breast milk syndrome. Insufficient breastfeeding syndrome can be treated with improvements in lactation management and consuming food sources that can affect the work of the hormone prolactin in producing breast milk. Food sources based on local wisdom that have been extensively studied for their effect on breast milk production include katuk leaves, moringa leaf extract, papaya leaves and fruit, banana heart, and mung bean extract. The purpose of carrying out this educational activity is a change in knowledge and a healthier attitude. The education carried out targets an increase in the knowledge and attitudes of the education participants with the output in the form of educational media, activity results reports and journal publications. Community Service Activities will be held on July 30 2022 starting with an opening (introduction, time contract, explanation of objectives and materials to be provided and distribution of pre-test questionnaires), followed by educating participants about local foods that can increase breast milk production and ending with closing ( distribution of leaflets, distribution of post-test questionnaires, and closing greetings). This activity was attended by approximately 15 participants at PMB Yustati Ambarita. The results of the evaluation during the educational process were that the participants seemed enthusiastic about the counseling material, the participants listened to the counseling carefully and the participants asked questions related to the material that was not clear. The results of the analysis of the pre and post test questionnaires on 10 participants, there was an increase in the participants' knowledge and attitudes from before the educational activity and after the educational activity. It is hoped that breastfeeding mothers can implement the knowledge gained in order to increase milk production by consuming local food that is around the community

Green synthesis of Moringa oleifera leaf nanoparticles and an assessment of their therapeutic potential

The present study centered around the prospect of bio fabricating nanoparticles using Moringa oleifera leaf and investigating their therapeutic potential with an assessment of the antioxidant status. Synthesis of stable ‘green’ nanoparticles of Moringa leaf extract is successfully reported with an extensive characterization. The average particle size and polydispersity index (PDI) of the nanoparticles obtained by Dynamic light scattering (DLS) analysis were 141.6 nm and a polydispersity index (PDI) of 0.32 respectively. The UV–visible spectroscopy (UV–vis) revealed a strong red absorbance peak at 664 nm,characteristic of chlorophyll. Electron micrographs confirmed that particles were in the nano range and were spherical with minimal agglomeration. The constituent functional groups were assessed by Fourier transformed infrared spectroscopy (FTIR). The X-ray diffractograms of reflected both the amorphous and crystalline domains.Furthermore, the phytochemical screening showed a higher phenolic/ flavonoid content of the Moringa nanoparticles (NMo) in comparison to the bulk Moringa leaf extract(Mo). These results corresponded to a potent antioxidant status of the NMo which was assessed by three complementary antioxidant assays. The therapeutic potential of the nanoparticles was evaluated based on in vitro anti-diabetic assays and cytotoxicity assay on human cancer cell lines (MCF-7 and HepG-2). The key findings suggest a more profound therapeutic efficacy of NMo than the Moringa leaf extract.

Alleviation of drought stress and mediated antioxidative defense in wheat through moringa leaf extract hormesis

Alleviation of drought stress and mediated antioxidative defense in wheat through moringa leaf extract hormesis

Aktivitas antimikroba, karakteristik fisikokimia, dan hedonik gel biosanitizer ekstrak daun kelor (Moringa oleifera) [Antimicrobial activity, physicochemical and sensory characteristic of Moringa oleifera leaf extract biosanitizer gel

Moringa leaves have bioactive compounds of flavonoids and tannins, which can be used as antimicrobials to substitute triclosan and glycerol compounds in commercial sanitizer gels. This study aims to determine the antimicrobial activity, physicochemical characteristics, and sensory properties of the moringa leaf extract of the bio-sanitizer gel. The research consisted of two stages: the extraction of Moringa leaves and the stages of making bio-sanitizer gel. The study used a factorial completely randomized design (CRD) with two factors: the type of solvent and the ratio of sample to solvent. Data were analyzed using variance (ANOVA) and Duncan's Range Test (DMRT). The results showed that all samples added to Moringa leaf extract had an antibacterial activity with inhibition zone values that did not differ between treatments within a range of 2.5-3.0 mm. All samples of Moringa leaf extract bio-sanitizer gel have the potential to inhibit E.coli and S.aureus bacteria. The treatment selected for this study was a bio-sanitizer gel of Moringa leaf extract with ethanol solvent and concentration of 1:6 with a pH value (3.90), viscosity (5.57), homogeneous, and having the preferred sensory value (aromatic = 6.47, color = 5.89, viscosity = 5.54, tackiness = 5.48, overall = 5.95).

Moringa Leaf Extract as a Natural Preservative against the Deterioration of Tilapia Fillet during Room Temperature Storage

This research was conducted to determine the percentage of moringa leaf extract to inhibit the deterioration of the quality of the tilapia fillet as a natural preservative against organoleptic characteristics during room temperature storage. This research was carried out at the Processing of Fishery Products Laboratory, the Aquaculture Laboratory of the Faculty of Fisheries and Marine Sciences, and the Central Laboratory of Padjadjaran University, Jatinangor. The research method used is experimental. Namely, a tilapia fillet soaked using different concentrations during room temperature storage. The organoleptic test (scoring test) was analyzed by Friedman's non-parametric statistical method consisting of 4 treatments and 20 semi-trained panelists as the number tests. In contrast, the pH testing of the tilapia fillet was analyzed by a descriptive method. Tilapia fillet with Moringa leaf extract soaking treatment consists of 0%, 4%, 8%, and 12% (v/v) for 30 minutes of soaking, then stored at room temperature (25°C-28°C). Observations occur once every 2 hours, namely at the 0, 2nd, 4th, 6th, 8th, 10th, 12th, 14th, and 16th hours. The parameters observed included organoleptic characteristics with 20 semi-trained panelists and a value of the tilapia fillet's degree of acidity (pH). The best use of moringa leaf extract at 8% appearance with an organoleptic value of 7.5, aroma of 7.7, and texture of 7.0 with a pH value of 6.8 to 16 hours storage.

Effect of 4% and 15% moringa leaf extract gel on gingival wound healing in rats

Traumatic dental procedures such as incisions and gingival flaps are the leading causes of injury to the gingival structure. One of natural ingredients that can potentially accelerate the wound healing process is Moringa leaves Moringa oleifera). Moringa leaf has several active compounds, one of which is flavonoids, which can be an antiinflammatory and antibacterial agent, and increase collagen synthesis. A randomized posttest-only control-group design was used in this study. 48 wistar rats were categorized into four groups: CMC-Na gel, hyaluronic acid, 4% Moringa leaf extract gel, and 15% Moringa leaf extract gel. The samples were then euthanized on days 1, 3, 5, and 7. The two-way ANOVA test described significant differences (p < 0.05) for all the components of the observation (neutrophils, fibroblasts, angiogenesis, and epithelial thickness). The lowest mean number of neutrophils, the highest mean number of fibroblasts, and the highest mean of angiogenesis were found in the 15% Moringa extract group on the 7th day. The highest mean epithelial thickness was found in the use of 4% Moringa extract on the 5thday. The microscopic images showed that the treatment group gained more effective wound healing processes than the control group. The microscopic image showed that, in terms of neutrophils, fibroblasts, and angiogenesis, 15% Moringa extract was more effective for wound healing compared to 4% Moringa extract.

Role of foliar spray of plant growth regulators in improving photosynthetic pigments and metabolites in Plantago ovata (Psyllium) under salt stress–A field appraisal

Salinity is one of the major abiotic factors that limit the growth and productivity of plants. Foliar application of plant growth regulators (PGRs) may help plants ameliorate the negative impacts of salinity. Thus, a field experiment was conducted at the Botanical Garden University of Balochistan, Quetta, to explore the potential role of PGRs, i.e., moringa leaf extract (MLE; 10%), proline (PRO; 1 µM), salicylic acid (SA; 250 µM), and thiourea (TU; 10 mM) in ameliorating the impacts of salinity (120 mM) on Plantago ovata, an important medicinal plant. Salinity hampered plant photosynthetic pigments and metabolites but elevated oxidative parameters. However, foliar application of PGRs enhanced photosynthetic pigments, including Chl b (21.11%), carotenoids (57.87%) except Chl a, activated the defense mechanisms by restoring and enhancing the metabolites, i.e., soluble sugars (49.68%), soluble phenolics (33.34%), and proline (31.47%), significantly under salinity stress. Furthermore, foliar supplementation of PGRs under salt stress led to a decrease of about 43.02% and 43.27% in hydrogen peroxide and malondialdehyde content, respectively. Thus, PGRs can be recommended for improved photosynthetic efficiency and metabolite content that can help to get better yield under salt stress, with the best and most effective treatments being those of PRO and MLE to predominately ameliorate the harsh impacts of salinity.

Antioxidant Activity, Texture Attributes, and Hedonic Properties of Fortified Cheese with Moringa Leaf Extract

Antioxidant Activity, Texture Attributes, and Hedonic Properties of Fortified Cheese with Moringa Leaf Extract

Growth performance and nutritional impact of Moringa oleifera leaf extract as supplemental diets in the juvenile pacific white shrimp (Penaeus vannamei)

Moringa (Moringa oleifera) is widely recognized for its potent medicinal properties and immunostimulant effects. This study aimed to investigate the impact of a hot water extract of moringa leaves on the growth performance, feed conversion ratio, and survival of pacific white shrimp, Penaeus vannamei. Four different diets were used in this experiment, each containing varying levels of moringa leaf extract: 0, 2.5, 5, and 7.5 g/kg of the diet. A total of 1,200 shrimp, with an initial body weight of 0.01 g/individual, were carefully selected to ensure they were free from Vibrio infestation. One hundred shrimp were placed in each circular tank, with a volume of 750 L or 133 individuals per cubic meter. The shrimp were fed the diets four times daily, with a protein content ranging from 13% to 39%, over a period of 30 days. The results of the study revealed that the growth performance and survival rate of the shrimp in the groups that were fed diets supplemented with moringa were significantly higher than those in the control group (without moringa supplementation). The highest growth performance, in terms of specific growth rate, weight gain, and final weight, was observed in the groups of shrimps that were fed diets containing either 2.5 g or 5 g of moringa leaf extract per kilogram of the diet (p<0.05). Furthermore, the diet containing 2.5 g of moringa leaf extract per kilogram resulted in the highest survival rate compared to the other treatment groups. Therefore, it can be concluded that the optimal level of moringa in the diet of vannamei shrimp is 2.5 g/kg of the diet.

Effect of moringa leaf extract and clove bud oil alone or in combination on growth performance, immunity and carcass characteristic of broiler chicken

Effect of moringa leaf extract and clove bud oil alone or in combination on growth performance, immunity and carcass characteristic of broiler chicken

Interactive potential effects of moringa leaf extracts and plant growth promoting rhizobacteria against aphid attack on a newly developed wheat variety

Interactive potential effects of moringa leaf extracts and plant growth promoting rhizobacteria against aphid attack on a newly developed wheat variety

Populasi Mikroba Rumen, Fermentabilitas, dan Kecernaan Suplementasi Daun Kelor dalam Ransum Sapi Perah secara In Vitro

This study aimed to evaluate the microbial rumen population, fermentability, and digestibility of Moringa leaf supplementation in dairy cow ration using in vitro and to determine the optimal level of supplementation. The experiment consist of two steps with the first step was microbiology measurement used a Randomized Block Design with 5 treatments level of Moringa leaf extract (P0= control; P1= 5%, P2 = 10%, P3 =15%, P4 =20%) and the second step was in vitro fermentability and digestibility measurement used Randomized Block Design with 7 treatments level of Moringa leaf in dairy cow ration (R0 = control, R1 = R0 + 2.5% Moringa leaf, R2= R0 +5% Moringa leaf, R3 = R0 + 7.5% Moringa leaf, R4= R0+10% Moringa leaf, R5=R0+12.5% Moringa leaf, R6=R0+15% Moringa leaf) which grouped by rumen fluids. Data analysis used analysis of variance and continued with Duncan’s Multiple Range Test. The measured variable were microbial rumen population (bacteria and protozoa), fermentability (N-NH3, VFA), microbial protein synthesis, dry matter digestibility (DMD), and organic matter digestibility (DMO). The results showed Moringa leaf extract significantly decreased bacterial population (p<0.05), but there was no effect on the protozoa population. Moringa leaf supplementation did not affect N-NH3, DMD, and DMO, but significantly influenced VFA concentration and microbial protein synthesis (p<0.01). The higher Moringa leaf supplementation showed decreasing total VFA concentration, but was still within the normal range for rumen fermentation (102.29-126.69 mM). Moringa leaf supplementation showed a quadratic effect on microbial protein synthesis with an optimal supplementation level of 5%, but decreasing at a level of 7.5% still within in normal range. It can be concluded Moringa leaf can be supplemented up to 7.5% in dairy cow ration.
 Key words: digestibility, fermentability, in vitro, moringa leaf, dairy cow

RESPONSE OF HIGH-YIELDING PEANUT CULTIVARS TO VARIOUS SEED TREATMENTS UNDER MARGINAL FERTILITY SANDY SOIL CONDITIONS

Ameliorating peanut production is a requirement to cope with the abrupt climate change and burgeoning population. Seed treatment is vital for enhancing and sustaining peanut production, particularly in semiarid environments. The latest study aimed to evaluate the impact of different seed treatments on the agronomic and quality of three high-yielding peanut cultivars: Giza-6, North Carolina (N.C.), and Aramanch. The applied seed treatments include Rhizobium inoculation, moringa leaf extract, vitavax, and gypsum versus untreated control. The evaluated peanut cultivars significantly varied in their results for agronomic and quality traits. The cultivar Giza-6, followed by Aramanch, proved the best displaying the highest number of seeds per pod, number of pods per plant, 100-seed weight, number of branches per plant, shelling percentage, biological yield, pod yield, seed yield, oil yield, and protein yield. The applied seed treatments substantially enhanced peanut yield traits, oil, and protein content of peanuts with the superiority of Rhizobium inoculation, gypsum, and moringa extract. These treatments effectively reinforced peanut growth, positively reflected in the yield and quality traits. Subsequently, integrating the seed treatments, particularly Rhizobium inoculation, gypsum, and moringa extract, with high-yielding cultivars, such as Giza-6 and Aramanch, confirmed a helpful approach to enhancing and sustaining peanut production in arid environments.

Effects of various plant growth regulators and sowing methods on the growth, yield and quality of rice (Oryza sativa L.)

Rice (Oryza sativa L) is an important source of food, as the population of the world is increasing the additional supply of rice is requisite to feed the population. Moringa leaf extract, salicylic acid and zinc sulphate are excellent growth regulators used worldwide. A field experiment was conducted on rice at Bahauddin Zakariya University, Multan, Pakistan. Fine rice (super basmati) was sown by two methods (S1= transplanting method and S2= direct-seeded rice) supplied with three growth regulators G1= moringa leaf extract (30 times diluted), G2= salicylic acid (50 mg per liter) and G3= zinc sulphate (50 g per liter) as foliar applications and G0devised as control treatment. Foliar application of growth regulators to respective treatments was done at two stages (before and after flowering). Data regarding growth (crop growth rate, photosynthetic rate and stomatal conductance), yield (number of grains per panicle, thousand grains weight, grain yield, biological yield and harvest index) and quality (normal kernel, sterile kernel, amylose contents, protein contents and brown rice rate) were studied. Compiled results of the study showed that moringa leaf extract along with the transplanting method performed better in most of the parameters than all other treatments. Transplanting with salicylic acid gave better results in the normal kernel, amylose contents and protein contents whereas zinc sulphate gave better results in brown rice rate.

FORMULASI NANOEKAPSULASI EKSTRAK DAUN KELOR (Moringa oleifera) /KITOSAN-NATRIUM TRIPOLIPOSFAT (NaTPP)

Moringa (Moringa oliefera) contains several antioxidant compounds such as quercetin and chlorogenic acid. However, antioxidants have weaknesses, namely that they are easily damaged by exposure to oxygen, light, high temperatures, and drying. One way to maintain and increase the stability of antioxidants is by encapsulation. The purpose of this study was to determine the optimum formulation of quercetin nanoencapsulation of moringa leaf extract using chitosan and sodium tripolyphosphate (Na-TPP) using the ultrasonication method. Moringa leaf extract was obtained by repeated ultrasonication 1x 15, 3x15, and 5x15 minutes, frequency 24 kHz, and using alcohol 96%. The encapsulation process was carried out with three variable ratios of the amount of chitosan: Na-TPP added to 10 mL of Moringa leaf extract solution (F0), namely 5:1 (F1), 2:1 (F2), and 1:1 (F3). Test results for flavonoids as quercetin using a UV-Vis spectrophotometer showed that the Quercetin content in Moringa leaf extract ultrasonicated 5x15 minutes was 12.146%, the characterization of the Particle Size Analyzer (PSA) showed encapsulated particle sizes of 170.3 and 209.7 nm with polydisperse index <0 .5, the antioxidant activity test using the DPPH method showed that F0 was 61.4% and F2 had the highest antioxidant activity, namely 81.2%. Analysis of the morphology of nanoparticles using Scanning Electron Microscopy (SEM) showed that the particles were spherically distributed evenly. Nanoencapsulation of Moringa leaf extract has resulted and an increase in the antioxidant activity of Moringa leaf extract after encapsulation using Chitosan-NaTPP, from 61.4% to 81.2% (F2).

Effect of Some Biostimulants on the Vegetative Growth, Yield, Fruit Quality Attributes and Nutritional Status of Apple

Although the application of chemical fertilizers to crops promotes plant growth and yield, their continuous use affects soil heath and creates environmental pollution. On the other hand, plant biostimulants improve nutrients absorption, plant growth, yield and produce quality and are environment-friendly. Therefore, an experiment was conducted during 2021-22 to evaluate the effect of some biostimulants on the performance of the apple cv. Anna, planted in a sandy loam soil at Marsa Matruh governorate, Egypt. Ninety trees were randomly selected and sprayed with 4 or 6% moringa leaf extract (MLE), 0.3 or 0.4% seaweed extract (SWE), 1000 or 2000 mg L−1 Fulvic acid (FA), 4% MLE + 0.3% SWE + 1000 mg L−1 FA (combination 1), or 6% MLE + 0.4% SWE + 2000 mg L−1 FA (combination 2) before flowering, during full bloom and one month later and compared with a control (untreated trees). The results demonstrated that spraying MLE, SWE or FA or their combinations positively improved the vegetative growth, fruit set %, fruit yield and fruit physical and chemical characteristics as well as leaf nutritional status. The positive effect of MLE, SWE and FA was increased in parallel to an increase in the used concentration of each one of them. The highest increments in the measured parameters were accompanied by the application of combination 2 over the other treatments.

Evaluation of Seed Germination of Barley Grown after Priming with Leaf Extract of Moringa and Aqueous Garlic Extract

A pot experiment was designed to determine the effect of seed priming on the germination of barley seeds in the winter season. Seed priming was done by using the leaf extract of Moringa at different concentrations (100%, 50% and 10%v/v) for 24 hours, and aqueous extract of ginger and tap water for different intervals of time (6, 12 and 24 hours). The experiment was continued for ten days with nine seed priming treatments. The results showed that maximum seeds germinated on day nine in all treatments. Whereas the highest germination of seeds was observed when seeds were primed with an aqueous extract of garlic for 24 hours and when seeds were primed with 100% concentration of Moringa leaf extract for 24 hours. The lowest seed germination was observed when seeds were primed with an aqueous extract of garlic for 6 hours. These results showed that Moringa leaf extract and aqueous garlic extract can be used to prime barley seeds to improve germination.

Effect of Panchagavya and Leaf Extracts of Moringa, Neem and Seaweed on Growth, Yield and Yield Attributing Traits of Spinach (Spinacia oleracea L)

The present investigation was carried out at Field Experimentation Centre, Department of Genetics and Plant Breeding, Sam Higginbottom University, Technology and Sciences, Prayagraj (UP) for “Effect of Panchagavya and Leaf Extracts of Moringa, Neem and Seaweed on Growth, Yield and Yield Attributing Traits of Spinach (Spinacia oleracea L) Variety-All Green”. The experiment was laid out in Randomized Block Design with thirteen treatments including control which were replicated thrice on spinach seed variety ‘All-Green’ were used to study under field conditions .The treatments were as follows, T0- Control, T1,T2,T3 – Panchagavya @ (3,5,7%) for 8Hrs, T4,T5,T6 – Neem leaf extract @ (3,5,7%) for 8hrs, T7,T8,T9 – Moringa leaf extract @(3,5,7%) for 8hrs, T10,T11,T12 – Seaweed liquid extract@ (2,4,6%) for 8hrs respectively. ANOVA given the significance difference among all treatments compared to control for all parameters under study. It is concluded from the present study that the seeds of Spinach variety – All Green treated with T10-Seaweed Liquid Extract@ 2% for 8hrs was recorded the highest Number of leaves(19), length of leaf (cm)(12.8), No. of seeds per plant(gm) (4.08), Number of seeds per plot (gm)(69.46), Biological yield per plant (g)(79.73), Harvest index(69.67) followed by T3 Panchagavya @ 7% for 8hrs producing number of leaves(18), length of leaf(cm)(11.2),No. of seeds per plant (gm) (3.71), Number of seeds per plot (gm)(63.05), Biological yield per plant (g)(73.56), Harvest index(65.10) which were highly significant at 5% level of significance.

Standardization of Recipe of Value-Added Functional Aloe Vera Drink Enriched with Moringa Leave

Abstract This study aimed to investigate the standard formulation of aloe Vera drinks using different moringa leaf extract levels and assess the quality parameters. The result showed the moisture content of the formulated samples was 99.85 to 99.80%, with higher in sample T2 and lowered in the control sample (T0). The protein content for all samples ranged from o.11 to 0.42%, with the highest found in sample (T3) and lowest found in sample T0. The pH was increased from 3.8 to 4.13 for all samples. The mean values of TSS of aloe Vera drink for all samples were ranged between 11.33 to 13.67 %. The acidity for all samples was in the ranges of 0.25 to 0.31%. The sensory attributes of drinks were evaluated in terms of color, flavor, taste, and overall acceptability. After increasing, the moringa leaves percentage; the consumer acceptance was gradually decreased. Overall, sample T1 containing 1% moringa leaves blended with 4% aloe Vera showed a reliable result compared to other samples.

Kualitas Spermatozoa Kambing Peranakan Boer yang Dipreservasi dengan Pengencer Air Kelapa Muda dan Ekstrak Daun Kelor

ABSTRACT
 Moringa leaves contain various nutrients that can protect spermatozoa during semen preservation. The purpose of this study was to examine the ability of moringa leaf extract with different concentrations of coconut water extender on the quality of Boer crossbreed goat spermatozoa preserved at 3-5°C. The treatment of this study was the concentration level of moringa leaf extract in coconut water extender. Fresh semen was divided into five tubes and diluted with 80% coconut water + 20% egg yolk (P0, control), 79% coconut water + 20% egg yolk + 1% moringa leaf extract (P1), 77% coconut water + 20% egg yolk + 3% moringa leaves ectract (P2), 75% coconut water + 20% egg yolk + 5% moringa leaf extract (P3), and 73% coconut water + 20% egg yolk + 7% moringa leaf extract (P4), respectively. The variables observed were motility and spermatozoa viability. Results of this study showed that supplementation of different concentrations of moringa leaf extracts in coconut water extender was significantly (P<0.05) on motility and viability of Boer crossbreed goat spermatozoa during preservation. Supplementation of 3% moringa leaf extract in coconut water extender was better than 1, 5, and 7% in maintain the quality of Boer crossbreed goat spermatozoa during preservation.
 Keywords: Moringa Leaf Extract, Semen Preservation, Boer Crossbreed Goat