To study the differences in material performance between HRB400E steel and conventional strength steel, this paper performed monotonic and cyclic loading tests on HRB400E and Q355B steels under a series of loading protocols. Additionally, the self-designed fixtures were used to achieve large strain rate loading. The ductility, monotonic behavior and hysteretic characteristics of HRB400E and Q355B steels were assessed, along with the energy dissipation capacity evaluated through the energy dissipation coefficients. The cyclic skeleton curves of both steels were fitted using the Ramberg-Osgood model, with further investigation into the influence of kinematic hardening parameters on hysteretic curves. The combined hardening parameters of the Voce-Chaboche model were calibrated based on the experimental results, and the hysteretic curves were simulated under different loading protocols via ANSYS. The analysis results reveal that the energy dissipation coefficients of HRB400E steel are larger than that of Q355B steel, yet smaller than those of Q460D, LYP100, LY100, LY160, and LY225 steels. Both HRB400E and Q355B steels demonstrate significantly improved material strength under cyclic loading. Excellent agreement between simulated and experimental curves is achieved when using four pairs of kinematic hardening parameters. Additionally, the hysteretic performance of HRB400E and Q355B steels under different cyclic loading protocols can be precisely simulated by employing calibrated combined hardening parameters. Through this paper, the cyclic behavior and constitutive relations of HRB400E steel are comprehensively understood, which provides a reliable theoretical basis for engineering design and structural analysis. Furthermore, this research provides a scientific basis for material applications of HRB400E steel.