Male Plants Research Articles

Reduced CHH methylation levels reveal a critical role of aging pathway genes in Moso bamboo flowering

Moso bamboo holds significant economic importance in China, serving various purposes, such as food, material, ornamentation, and greenery. Despite its versatility, the occurrence of flowering in Moso bamboo poses a threat to bamboo forests, resulting in substantial losses. The underlying cause of bamboo flowering remains elusive. Dynamic fluctuations in DNA methylation govern the transcriptional levels of crucial genes pivotal for plant growth and development. In this study, we conducted comprehensive DNA methylation (by whole-genome bisulfite sequencing) and transcriptome (by RNA-seq) analyses on non-flowering leaves, flowering leaves, and spikelets of Moso bamboo. Our findings revealed a notable reduction in the overall DNA methylation level, particularly CHH methylation, from leaves to spikelets, influencing the expression of differentially regulated genes. Notably, we identified DNA methylation as a regulatory mechanism for numerous flowering-related genes, including SPLs, FT, and SOC1. Specifically, the SPL3f gene, a key regulator of the aging pathway, exhibited hypomethylation and a high expression level in spikelets. Conversely, SOC1c displayed transcriptional silencing attributed to hypermethylation in the CHH context in the leaves of non-flowering plants. DNA methylation may affect the flowering mechanism of Moso bamboo by regulating the expression of key genes. In summary, our results shed light on the dynamic changes in DNA methylation between leaves and spikelets, unraveling an important epigenetic modification mechanism for flowering in Moso bamboo.

Morphogenesis, megagametogenesis, and microgametogenesis in Actinidia arguta flower buds

In dioecious plants, understanding sexual differentiation is important for improving cultivation techniques. Flower bud morphogenesis and gametophyte formation underlie the mechanism of sexual differentiation. Actinidia arguta (hardy kiwi) is a unisexual plant, and there have been few studies on its flower bud differentiation or gametophyte formation. We studied flower bud morphogenesis and gametophyte formation in A. arguta using paraffin sections and found that the morphogenesis of male and female flower buds was divided into six stages: the undifferentiated stage, inflorescence initials differentiation stage, calyx primordium differentiation stage, petal primordium differentiation stage, stamen primordium differentiation stage, and pistil primordium differentiation stage. Morphogenesis in the male inflorescence can reach quaternary flower primordia, and morphogenesis in the female inflorescence can reach the tertiary flower primordia, however, no Stage 4 lateral flowers in the male inflorescence or Stage 3 lateral flowers in the female inflorescence were found in natural situation. The embryo sac in female plants was a monocellular Polygonum type, and the embryo sac development in male plants was incomplete; abortion occurred before the archesporial cells developed. The male gametophyte differentiation process in the male plant was normal, with pollen grains similar in shape to a rugby ball and arranged neatly in the anther. The pollen grain differentiation in female plants was abnormal; abortion occurred at the uninucleate microspore stage, and mature pollen grains were irregularly spherical.

Assessment of sex, nut weight, germination vigour, stem growth, and development traits of pistachio cultivars (Pistacia vera L.) used as rootstock

ABSTRACT Pistacia vera L. is the only edible plant with economic importance within Pistacia genus. In the current study, rootstock traits such as growth and development of major pistachio cultivars were evaluated in plants used as rootstock for pistachios in Turkey. The sex of 1008 individuals was determined using a molecular sex marker with HRM analysis. From the eight cultivars, the number of female and male plants were 453 and 413, respectively; and then used for phenotypic measurements. For each measurement period, analysis of variance established that there were no significant ‘cultivar x sex’ interactions or sex effects for stem diameter or stem height. However, the differences among cultivars were highly significant (P < 0.0001) at all measurement times. The maximum stem length and width values were in the progeny of the Ohadi cultivar, with the lowest values recorded for progeny of the Uzun and Kırmızı cultivars. The nut weights and stem properties of the plants were correlated with high positive coefficients, although the germination vigour was not statistically correlated with any other trait. The results of the current paper will aid the selection of candidates for future pistachio rootstock breeding programmes.

DWARF27 and CAROTENOID CLEAVAGE DIOXYGENASE 7 genes regulate release, germination and growth of gemma in Marchantia polymorpha.

Strigolactones (SLs), a class of carotenoid-derived hormones, play a crucial role in flowering plants by regulating underground communication with symbiotic arbuscular mycorrhizal fungi (AM) and controlling shoot and root architecture. While the functions of core SL genes have been characterized in many plants, their roles in non-tracheophyte plants like liverworts require further investigation. In this study, we employed the model liverwort species Marchantia polymorpha, which lacks detectable SL production and orthologs of key SL biosynthetic genes, including CAROTENOID CLEAVAGE DIOXYGENASE 8 (CCD8) and MORE AXILLARY GROWTH 1 (MAX1). However, it retains some SL pathway components, including DWARF27 (D27) and CCD7. To help elucidate the function of these remaining components in M. polymorpha, knockout mutants were generated for MpD27-1, MpD27-2 and MpCCD7. Phenotypic comparisons of these mutants with the wild-type control revealed a novel role for these genes in regulating the release of gemmae from the gemma cup and the germination and growth of gemmae in the dark. Mpd27-1, Mpd27-2, and Mpccd7 mutants showed lower transcript abundance of genes involved in photosynthesis, such as EARLY LIGHT INDUCED (ELI), and stress responses such as LATE EMBRYOGENESIS ABUNDANT (LEA) but exhibited higher transcript levels of ETHYLENE RESPONSE FACTORS (ERFs) and SL and carotenoid related genes, such as TERPENE SYNTHASE (TS), CCD7 and LECITHIN-RETINAL ACYL TRANSFERASE (LRAT). Furthermore, the mutants of M. polymorpha in the SL pathway exhibited increased contents of carotenoid. This unveils a previously unrecognized role for MpD27-1, MpD27-2 and MpCCD7 in controlling release, germination, and growth of gemmae in response to varying light conditions. These discoveries enhance our comprehension of the regulatory functions of SL biosynthesis genes in non-flowering plants.

Comparison of functional properties of unripe papaya fruits of different sexes.

Papaya (Carica papaya L.) is a herbaceous plant belonging to the family Caricaceae in the order Brassicales. The shape of papaya fruit was linked to sex, and the fruit of female plants is round, whereas that of hermaphrodites is pyriform. Although fruit shape preferences vary by region, differences in their functionalities have not been investigated. Since unripe fruit, also called green papaya, is known for its nutritional and therapeutic benefits, we performed a metabolome analysis of unripe papaya using liquid chromatography coupled with quadrupole/time of flight mass spectrometry. We first focused on capraine derivatives, major piperidine alkaloids, and bioactive compounds with significant antiplasmodial activity. Interestingly, carpaine derivatives tended to be altered in the peel and pulp but not in the seed. Multivariate analyses indicated little difference or minor differences to the extent that they can be caused by individual differences in metabolite profiling between the two sexes. Conversely, total polyphenol content and proteolytic activity were also investigated, but there were no differences between females and hermaphrodites for total polyphenol content and proteolytic activity. In conclusion, the metabolome and major functionalities were similar between hermaphrodites and female unripe fruit. However, it would be worth considering the sex of the material fruit, especially when focusing on the functional properties of carpaine derivatives.

Isolation and evaluation of novel male sterile and self‐incompatible mutant lines of mungbean (Vigna radiata L.)

AbstractHeterosis has long been harnessed in crop production, and while crop fertility lays the groundwork for leveraging heterosis, there remains a dearth of comprehensive data regarding genic male sterility in mungbean. Mungbean (Vigna radiata (L.) Wilczek), being a self‐pollinating crop, exhibits significant hybrid vigour in F1 hybrid seed yield, suggesting the potential for developing hybrid varieties to propel mungbean yield beyond existing plateaus. A new male sterile mutant was induced, isolated, stabilized and evaluated, via gamma irradiation at a rate of 600 Gy, identified in the M3 generation of the GM 4 accession. A uniform male sterile line was crossbred with the wild‐type parental plant to explore the inheritance pattern of male sterility. Pollen sterility was validated through the acetocarmine test, while stigma viability was ascertained using the hydrogen peroxide. While all F1 plants displayed fertile flowers, the F2 generation showed a clear 3:1 segregation ratio for fertile to male sterile plants, indicating the control of male sterility by a single recessive gene, mms. Also, a novel existence of a truncated stigma nestled within the anther column inhibits the effective reception of pollen during anthesis, offering potential in reducing emasculation time by a slight cut on the flower bud and enhancing cross pollination during hybrid seed production. The isolation of these two mutants is poised to significantly advance the global mungbean hybrid breeding programme.

Grab to fill the gap: key factors influencing Reynoutria japonica germination and seedling establishment in the secondary distribution range

Reynoutria japonica (Japanese knotweed) is a highly invasive female plant that primarily reproduces through vegetative regeneration during secondary distribution. Despite producing a substantial number of viable hybrid seeds, the occurrence of seedlings and young plants in the wild remains rare; the reasons for this rarity are unclear. Environmental intolerance and chromosomal anomalies during hybridisation can impede seedling development. This study evaluates the combined impact of these factors by examining substrate, temperature, and light effects on germination, as well as assessing seedling genome sizes and viability. Seeds were cultivated in both natural and controlled environments to manage external influences. Flow cytometry was employed to evaluate chromosomal arrangements. The final germination was notably high at 99%, and 68% of seedlings thrived under controlled conditions, emphasising hybrid seedling viability regardless of highly polyploid levels ranging from pentaploid to aneuploid (2C DNA genome size from 5.17 to 11.95 pg). Thus, it is evident that seeds can germinate and produce vital seedlings despite various chromosomal sets. Even fluctuating temperatures and type of substrate do not limit seed germination. However, these results were obtained under laboratory conditions, with seeds and seedlings receiving regular irrigation. A significant seedling mortality rate (99%) was observed in the field experiment, and the final germination was also low there (15%). Observations suggest that water stress might be the cause of this mortality. Further research on water stress is necessary because it could be the primary factor limiting the successful generative spread of the knotweeds.

Sex-specific strategies of resource utilization and determining mechanisms of Hippophae rhamnoides in response to community succession

Abstract The dioecious plant, Hippophae rhamnoides, is a pioneer species in community succession on the Qinghai-Tibet Plateau (QTP), plays great roles in various ecosystem services. However, the males and females of the species differ both in their morphology and physiology, resulting in a change in the ratio of male to female plants depending on the environment. To further explore the functional traits critical to this sex-based distinctive response in the alpine grassland, we have surveyed the sex ratios, measured their photosynthetic parameters, height, leaf area and biomass allocation. The results showed that (i) The males had higher Pn, light saturation point, apparent quantum efficiency, Amax and lower water-use efficiency (WUE), which exhibited higher utilization efficiency or tolerance to strong light, while the females indicated higher utilization efficiency for low light and water. And it showed sex-specific biomass allocation patterns. (ii) H. rhamnoides populations across the successional stages all showed a male-biased sexual allocation, which was closely related to sex-specific WUE, Pn, root biomass/total biomass and root–crown ratio. (iii) The leaf traits of H. rhamnoides changed from higher Narea, Parea and leaf mass per area in the early and late to lower in the middle, which meant they moved their growth strategy from resource rapid acquisition to conservation as the succession progressed. (iv) The increasing soil total phosphorus mostly contributed to regulating the sex bias of populations and variations of traits during the succession. The results are vital for the management of grassland degradation and restoration due to shrub encroachment on the QTP.

CRUZAS SIMPLES PROGENITORAS E HÍBRIDO TRILINEAL DE MAÍZ H-53 AE: DENSIDADES Y PRODUCTIVIDAD

&lt;p&gt;&lt;strong&gt;Background&lt;/strong&gt;. The use of improved seeds in Mexico is limited. To increase its use and improve its supply, it is necessary to promote the generation of micro-enterprises; for this, the Universidad Nacional Autónoma de México (UNAM) and Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) work in a coordinated manner on the development of new varieties. &lt;strong&gt;Objective&lt;/strong&gt;. To determine the seed productivity by effect of the population density of the trilinear hybrid of H-53 AE maize and its simple progenitor crosses in two environments of Valles Altos. &lt;strong&gt;Methodology&lt;/strong&gt;. In the spring-summer cycle of 2014, in Cuautitlán Izcalli and Texcoco, State of Mexico, three genotypes were evaluated: the simple female cross androsterile (M53AE x M59) and fertile version (M53F x M59), as well as the trilineal hybrid H-53 AE. Population densities were 55,000, 70,000, and 85,000 ha&lt;sup&gt;-1&lt;/sup&gt; plants, respectively. The variables evaluated were male and female flowering, plant and ear height, volumetric weight, weight of 200 grains, ear length, grains per row, dry matter percentage, and grain percentage. The experimental design employed a factorial design with random complete blocks, and the Tukey method was used for mean comparison. &lt;strong&gt;Results&lt;/strong&gt;. The simple crosses, androsterile and fertile, exceeded the performance of the hybrid trilineal H-53 AE (7280 Kg ha&lt;sup&gt;-1&lt;/sup&gt;). The population density where genotypes expressed a higher yield was 70, 000 ha&lt;sup&gt;-1&lt;/sup&gt; plants, with 8602 Kg ha&lt;sup&gt;-1&lt;/sup&gt;, significantly different to 7813 Kg ha&lt;sup&gt;-1&lt;/sup&gt;, and 7748 Kg ha&lt;sup&gt;-1&lt;/sup&gt; for 85,000 and 55, 000 ha&lt;sup&gt;-1&lt;/sup&gt; plants, respectively. &lt;strong&gt;Implications&lt;/strong&gt;. Further analysis of other environmental factors should be carried out to strengthen the recommendation of the evaluated trilinear hybrid. &lt;strong&gt;Conclusion. &lt;/strong&gt;The best responses were presented in the population density of 70,000 plants ha&lt;sup&gt;-1&lt;/sup&gt;, and the highest average yield of three genotypes and three population densities with 8768 Kg ha&lt;sup&gt;-1&lt;/sup&gt; was in the FESC environment (Cuautitlán Izcalli).&lt;/p&gt;

Enhancing temperature resilience in dioecious Salix myrtillacea: The role of acetic acid in eco-physiological and microbial adaptations

The effects of climate change on dioecious plants are of growing concern. Dioecious plants have sex-specific eco-physiological responses. This study investigated the response of Salix myrtillacea, a dioecious wood-producing plant with industrial potential, to high (35°C) and low (15°C) temperature stresses. The efficacy of exogenously applied acetic acid (AA) in enhancing sex-specific temperature stress tolerance was also investigated. The research found that female willows showed greater resilience to both temperature extremes than males. The average stress intensity indices for height and basal diameter growth rate were 19.20 % and 10.87 % higher in males than in females, respectively. This was also evidenced by greater physiological adaptations (antioxidant enzyme activity, hormone accumulation) and nutrient assimilation. Compared with high temperature, willows showed better adaptation to low temperature, resulting in a significant stress reduction. In addition, the application of AA significantly reduced stress in both sexes, while distinct sex-specific differences were observed in the composition of the rhizosphere microbial community. The application of AA particularly influenced certain microbial phyla. For example, it increased the relative abundance of Bacteroidetes by 46.11 % and Chloroflexi by 42.16 %, while decreased Acidobacteria by 29.28 %. This supported carbon uptake and storage to improve stress tolerance in females under high temperature stress. Conversely, males showed a stronger association with fungi to maintain stability. In conclusion, the application of AA significantly improved the temperature stress resistance of dioecious willows. There were pronounced improvements in growth, physiological adaptations, and microbial interactions. These effects were particularly evident in female plants. These findings highlight the potential of AA in tailoring climate resilience strategies for dioecious species.

GENETIC INSIGHTS AND BREEDING IMPLICATIONS: ENHANCING COTTON YIELD AND QUALITY THROUGH BIPARENTAL SELECTION

This study was performed in Egypt's Sakha Agriculture Research Station during the seasons 2015–2018 to study the efficiency of biparental progenies derived from inter-population random mating in F2 generation in a cotton cross. Intercrossing is allowed in cotton breeding populations. Although the best lines may include harmful alleles, thus early segregating generation recombination and hybridization are required. The F2 generation of the cotton cross (Giza70 TNB1), which obtained from Agriculture Research Center, Egypt, consisted of five male and eight female plants, was used to test biparental progeny from random interpopulation mating. About 28 and 24 families in BIPS1 and BIPS2 had their original parents were examined. All genotypes were assessed for agricultural and fiber qualities. The mean of families (BIPs2) was higher than BIPs1 for all measured metrics except lint yield, percentage, and uniformity ratio. BIPS2 families had more genotypic coefficients of variation (GCV) for features than BIPS1. Intercrossing affected correlation coefficients of BIPS1 and BIPS2 genotypes. Seed cotton yield and lint index genotypic correlations became considerable. Seed cotton yield estimated by seed index and first fruiting node location were altered from negative to positive. Among them, No.5, No.7, No.8, No.18, and No.19 had large yields and early ripening, as shown by their components. The top five families chosen for fiber length had gain values of 3.373% to 8.961% and 5.919 to 7.781% for fiber strength. The No.18 family exhibited good seed cotton production and fiber length. Intermating and self-pollination (BIPS2) may utilize non-additive gene effects and accumulate trait-influencing alleles. Continuing to select and evaluate families based on their large-scale reproductive performance will enhance the likelihood of producing genotypes with desired features. In conclusion, this study underscores the utility of continued selection based on discerned genetic parameters, genotypic correlations, and observed gains in facilitating the ongoing improvement of cotton breeding programs. By providing valuable insights into the intricacies of genetic variability and trait associations, this research holds implications not only for the advancement of cotton breeding practices but also for the broader agricultural landscape on both national and global scales. Keywords: Biparental, selection efficiency, correlated response, hybridization, self-pollination

Host-plant sex and phenology of Buddleja cordata Kunth interact to influence arthropod communities.

Intraspecific variation in plants is expected to have profound impacts on the arthropod communities associated with them. Because sexual dimorphism in plants is expected to provide consistent variation among individuals of the same species, researchers have often studied the effect it has on associated arthropods. Nevertheless, most studies have focused on the effect of sexual dimorphism in a single or a few herbivores, thus overlooking the potential effects on the whole arthropod community. Our main objective was to evaluate effects of Buddleja cordata's plant-sex on its associated arthropod community. We surveyed 13 pairs of male and female plants every 2 months during a year (June 2010 to April 2011). Every sampling date, we measured plant traits (water content and leaf thickness), herbivory, and the arthropod community. We did not find differences in herbivory between plant sex or through time. However, we found differences in water content through time, with leaf water-content matching the environmental seasonality. For arthropod richness, we found 68 morphospecies associated with female and 72 with male plants, from which 53 were shared by both sexes. We did not observe differences in morphospecies richness; however, we found sex-associated differences in the diversity of all species and differences on the diversity of the most abundant species with an interesting temporal component. During peak flowering season, male plants showed higher values on both parameters, but during the peak fructification season female plants showed the higher values on both diversity parameters. Our research exemplifies the interaction between plant-phenology and plant-sex as drivers of arthropod communities' diversity, even when plant sexual-dimorphism is inconspicuous, and highlighting the importance of accounting for seasonal variation. We stress the need of conducting more studies that test this time-dependent framework in other dioecious systems, as it has the potential to reconcile previous contrasting observations reported in the literature.

Gene Regulatory Network Controlling Flower Development in Spinach (Spinacia oleracea L.).

Spinach (Spinacia oleracea L.) is a dioecious, diploid, wind-pollinated crop cultivated worldwide. Sex determination plays an important role in spinach breeding. Hence, this study aimed to understand the differences in sexual differentiation and floral organ development of dioecious flowers, as well as the differences in the regulatory mechanisms of floral organ development of dioecious and monoecious flowers. We compared transcriptional-level differences between different genders and identified differentially expressed genes (DEGs) related to spinach floral development, as well as sex-biased genes to investigate the flower development mechanisms in spinach. In this study, 9189 DEGs were identified among the different genders. DEG analysis showed the participation of four main transcription factor families, MIKC_MADS, MYB, NAC, and bHLH, in spinach flower development. In our key findings, abscisic acid (ABA) and gibberellic acid (GA) signal transduction pathways play major roles in male flower development, while auxin regulates both male and female flower development. By constructing a gene regulatory network (GRN) for floral organ development, core transcription factors (TFs) controlling organ initiation and growth were discovered. This analysis of the development of female, male, and monoecious flowers in spinach provides new insights into the molecular mechanisms of floral organ development and sexual differentiation in dioecious and monoecious plants in spinach.

Knowing the invader: increasing knowledge about an invasive plant to improve management

Pampas grass is an invasive alien plant with negative consequences for ecosystems, human health, and economy. Although diverse aspects of its biology and the ecology have been studied, some information relevant to its management is still missing. We studied the ecological characteristics of seeds and plants relevant to management and habitat restoration, namely morphological and reproductive traits in female and hermaphrodite plants, flower development, seed longevity, and germinability under different conditions. Female and hermaphrodite plants showed significant differences regarding reproductive traits, but not for morphological traits. Female plants are largely responsible for the dispersal of the species, while hermaphrodite plants primarily serve as pollen donors, contributing to off‐season allergies. In general, seeds of female plants are mature 3–4 weeks from the onset of flower development and are dispersed by wind until after 4 months, but timings are influenced by weather conditions. The species is very adaptable and can germinate in different soils (85% in fluvisols, 65% in podzols, and 35% in solonchak), water salinities (77 and 75% in freshwater, 28 and 38% in mesohaline, and 7 and 35% in polihaline waters, in laboratory and field, respectively), and moisture conditions (50 and 33% in soaked conditions, and 25 and 80% in moistened conditions, in laboratory and field, respectively). Seeds lack dormancy, are short‐lived (up to 1 year in the field and 3 years in optimal storage conditions), and are easily destroyed at 40°C temperatures in dark conditions. Implications of these findings for the management of the species are discussed.

TRPV1-Dependent Antiproliferative Activity of Dioecious Maclura pomifera Extracts in Estrogen Receptor-Positive Breast Cancer Cell Lines Involves Multiple Apoptotic Pathways.

Globally, breast cancer is a significant cause of mortality. Recent research focused on identifying compounds regulating the transient receptor potential vanilloid 1 (TRPV1) ion channel activity for the possibility of developing cancer therapeutics. In this study, the antiproliferative properties and mechanisms of action through TRPV1 of Maclura pomifera, a dioecious tree native to the south-central USA, have been investigated. Male and female extracts of spring branch tissues and leaves (500 µg/mL) significantly reduced the viability of MCF-7 and T47D cells by 75-80%. M. pomifera extracts induced apoptosis by triggering intracellular calcium overload via TRPV1. Blocking TRPV1 with the capsazepine antagonist and pretreating cells with the BAPTA-AM chelator boosted cell viability, revealing that M. pomifera phytochemicals activate TRPV1. Both male and female M. pomifera extracts initiated apoptosis through multiple pathways, the mitochondrial, ERK-induced, and endoplasmic reticulum-stress-mediated apoptotic pathways, demonstrated by the expression of activated caspase 3, caspase 9, caspase 8, FADD, FAS, ATF4, and CHOP, the overexpression of phosphorylated PERK and ERK proteins, and the reduction of BCL-2 levels. In addition, AKT and pAKT protein expressions were reduced in female M. pomifera-treated cells, revealing that female plant extract also inhibits PI3K/Akt signaling pathways. These results suggest that phytochemicals in M. pomifera extracts could be promising for developing breast cancer therapeutics.

RESPUESTA DEL MAIZ A LA APLICACIÓN DE CONSORCIO MICROBIANO Y SU COMBINACION CON BIOL, EN CONDICIONES DE TRÓPICO SECO

&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;The utilization of organic fertilizers is put forward as an effective approach to address soil degradation and enhance crop yields. &lt;strong&gt;Objective:&lt;/strong&gt; To assess the impact of applying microbial consortia, in combination with fermented liquid fertilizer (biol), on maize grain production, with a focus on fostering sustainable family farming in dry tropical conditions&lt;strong&gt;. Methodology:&lt;/strong&gt; A randomized complete block design with three blocks and divided plots was employed, with the main plots featuring various maize cultivars, while the subplots consisted of four bioferments and a control treatment. The bioferments included Microbial Consortium (CM), BIOL+CM, and BIOL+EM-1, compared to the commercial product Efficient Microorganisms (EM-1) and a control with no application. Parameters such as days to male and female flowering, plant and ear height, leaf area, grain diameter, and yield, along with the physical and chemical soil characteristics, were evaluated following the bioferment application. &lt;strong&gt;Results:&lt;/strong&gt; The application of BIOL+CM to the Marginal 28T variety resulted in increased plant height (164.17 cm) and cob size (65.83 cm), as well as a larger leaf area (361.17 cm²). It also enhanced the grain yield of the Marginal 28T variety (3.42 tꞏha&lt;sup&gt;-1&lt;/sup&gt;) and the HS-1 hybrid (3.02 tꞏha&lt;sup&gt;-1&lt;/sup&gt;). &lt;strong&gt;Implications:&lt;/strong&gt; The combined use of locally-sourced microbial consortia and Biol significantly improves the agronomic performance of hard yellow corn and aids in maintaining the soil's physical and chemical conditions, thereby promoting its enhancement. &lt;strong&gt;Conclusion:&lt;/strong&gt; The Marginal 28T variety and the HS-1 hybrid exhibited a favorable response to the application of microbial consortia, particularly BIOL+CM, making it a viable option for family farming in dry tropical conditions.&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;

The holocentricity in the dioecious nutmeg (Myristica fragrans) is not based on major satellite repeats

Holocentric species are characterized by the presence of centromeres throughout the length of the chromosomes. We confirmed the holocentricity of the dioecious, small chromosome-size species Myristica fragrans based on the chromosome-wide distribution of the centromere-specific protein KNL1, α-tubulin fibers, and the cell cycle-dependent histone H3 serine 28 phosphorylation (H3S28ph) mark. Each holocentromere is likely composed of, on average, ten centromere units, but none of the identified and in situ hybridized high-copy satellite repeats is centromere-specific. No sex-specific major repeats are present in the high-copy repeat composition of male or female plants, or a significant difference in genome size was detected. Therefore, it is unlikely that M. fragrans possesses heteromorphic sex chromosomes.

Children's food allergy: Effects of environmental influences and antibiotic use across critical developmental windows

BackgroundIncreasing studies linked outdoor air pollution (OAP), indoor environmental factors (IEFs), and antibiotics use (AU) with the first wave of allergies (i.e., asthma, allergic rhinitis, and eczema), yet the role of their exposures on children’s second wave of allergy (i.e., food allergy) are unknown. ObjectivesTo investigate the association between exposure to OAP and IEFs and childhood doctor-diagnosed food allergy (DFA) during the pre-pregnancy, prenatal, early postnatal, and current periods, and to further explore the effect of OAP and IEFs on DFA in children co-exposed to antibiotics. MethodsA retrospective cohort study involving 8689 preschoolers was carried out in Changsha, China. Data on the health outcomes, antibiotic use, and home environment of each child were collected through a questionnaire. Temperature and air pollutants data were obtained from 8 and 10 monitoring stations in Changsha, respectively. Exposure levels to temperature and air pollutants at individual home addresses were calculated by the inverse distance weighted (IDW) method. Multiple logistic regression models were employed to assess the associations of childhood DFA with exposure to OAP, IEF, and AU. ResultsChildhood ever doctor-diagnosed food allergy (DFA) was linked to postnatal PM10 exposure with OR (95% CI) of 1.18 (1.03–1.36), especially for CO and O3 exposure during the first year with ORs (95% CI) = 1.08 (1.00–1.16) and 1.07 (1.00–1.14), as well as SO2 exposure during the previous year with OR (95% CI) of 1.13 (1.02–1.25). The role of postnatal air pollution is more important for the risk of egg, milk and other food allergies. Renovation-related IAP (new furniture) and dampness-related indoor allergens exposures throughout all time windows significantly increased the risk of childhood DFA, with ORs ranging from 1.23 (1.03–1.46) to 1.54 (1.29–1.83). Furthermore, smoke-related IAP (environmental tobacco smoke [ETS], parental and grandparental smoking) exposure during pregnancy, first year, and previous year was related to DFA. Additionally, exposure to pet-related indoor allergens (cats) during first year and total plant-related allergens (particularly nonflowering plants) during previous year were associated with DFA. Moreover, exposure to plant-related allergy during first and previous year was specifically associated with milk allergy, while keeping cats during first year increased the risk of fruits/vegetables allergy. Life-time and early-life AU was associated with the increased risk of childhood DFA with ORs (95% CI) = 1.57 (1.32–1.87) and 1.46 (1.27–1.67), including different types food allergies except fruit/vegetable allergy. ConclusionsPostnatal OAP, life-time and early-life IEFs and AU exposure played a vital role in the development of DFA, supporting the "fetal origin of childhood FA" hypothesis.

Selection assessment of promising forms of natural Hippophae rhamnoides (Elaeagnaceae) populations and their offspring in the Kazakhstan Altai Mountains

Abstract. Vdovina TA, Isakova EA, Lagus OA, Sumbembayev AA. 2024. Selection assessment of promising forms of natural Hippophae rhamnoides (Elaeagnaceae) populations and their offspring in the Kazakhstan Altai Mountains. Biodiversitas 25: 1809-1822. This article presents the results of studying the genetic diversity of sea buckthorn, Hippophae rhamnoides L. in natural populations of the East Kazakhstan region. This made it possible to identify valuable forms based on which targeted breeding work was carried out. Selection work has shown that this species has a unique gene pool. As a result of the analytical selection of H. rhamnoides in the first, second, and third generations, promising seedlings were obtained: ´Podarok Bajtulinu Sh-9-81 (4-6) II´, ´Jubilejnaja Kotuhova T-2-82 (2-22) III´, ´Dolgozhdannaja ?5 (3-24) III´, ´Shetlastinka ?7 (2-24) III´, ´Nesravnennaja Sh-9-81 (3-27) III´, ´Krasavica Sh-9-81 (3-30) III´, ´Solnyshko Sh-12-81 (1-18) III´ with high yield, large fruits, long stalk and low fruit tearing force. Among the male plants, the following forms are distinguished: ´Gustoj Tuman T-2-82 (1-24) III´, ´Ljubimec T-17-82 (2-20) III´, ´Krasavchik K-8-82 (1-34) III´, ´Bogatyr' T-17-82 (2-20) III´ with high winter hardiness and pollen productivity. A scale has been developed for the force of fruit separation and the density of the “cob” for local forms. A selection achievement (variety) patent was received for the ´Jubilejnaja Kotuhova´ form. The next two forms (´Shetlastinka´ and ´Podarok Bajtulinu´) pass the state test for economic usefulness. The richest gene pool of sea buckthorn in the Altai mountainous country indicates its high heterogeneity.

Harmful self-pollination drives gynodioecy in European chestnut, a self-incompatible tree.

Gynodioecy is a rare sexual system in which two genders (sensu Lloyd, 1980), cosexuals and females, coexist. To survive, female plants must compensate for their lack of siring capacity and male attractiveness. In European chestnut (Castanea sativa), an outcrossing tree, self-pollination reduces fruit set in cosexual individuals because of late-acting self-incompatibility and early inbreeding depression. Could this negative sexual interaction explain the presence of females in this species? We studied gender variation in wild populations of European chestnut. In addition, we compared fruit set (the proportion of flowers giving fruits) and other key female fitness components as well as reproductive allocation between genders. We then performed emasculation experiments in cosexual trees, by removing nectar-producing fertile male inflorescences. We also removed sterile but nectar-producing male inflorescences from female trees, as a control. We found a highly variable proportion of male-sterile individuals in the wild in European chestnut. In the experimental plot, trees from each gender had similar size, flower density, and burr set, but different fruit set. Removing nectar-producing male inflorescences from branches or entire trees increased fruit set in cosexual but not in female trees. These results show that self-pollination impairs fruit set in cosexual trees. Female trees avoid these problems as they do not produce pollen but continue to attract pollinators thanks to their rewarding male-sterile inflorescences, resulting in a much higher fruit set than in cosexuals. This demonstrates that even outcrossed plants can benefit from the cessation of self-pollination, to the point that unisexuality can evolve.