A major challenge for mid and high-rise timber buildings is fulfilling serviceability requirements. Moment-resisting timber frames with semi-rigid moment-resisting connections as the primary lateral load-resisting system can be an alternative to buildings with wind bracing or shear walls, allowing for more free space, enhanced building performance, and architectural flexibility. Moreover, introducing semi-rigid timber connections can improve the performance of floors against human-induced vibrations and potentially decrease the material used. The performance of moment-resisting frames depends mainly on the properties of their beam-to-column connections. The paper presents an experimental investigation of semi-rigid moment-resisting connections with long screwed-in threaded rods and a steel coupling part. Long threaded rods (i.e., screwed-in threaded rods with wood screw threads) feature high axial stiffness and resistance and allow for immediate load transfer without initial slip. Due to these properties, threaded rods are preferred over conventional dowel-type fasteners such as bolts and dowels. Five full-scale moment-resisting timber connections were tested under cyclic and monotonic loading to investigate moment resistance, rotational stiffness, and energy dissipation. The stiffness obtained by cyclic and monotonic tests was compared with numerical and analytical methods. The beam-to-column moment-resisting connections demonstrated a high moment resistance of 92.2 kNm and rotational stiffness of 8049 kNm/rad without initial slip. The experimental results and analytical calculations of rotational stiffness differed only by 5 %, whereas the finite element model exhibited a discrepancy of approximately 35 %. The equivalent viscous damping was determined based on cyclic load tests at service load levels and was found to be in the range of 5.5 %–10.0 %. Finally, a parametric study is presented to discuss the effect of semi-rigid connections on the performance of the timber floors and material savings. The increase in rotational stiffness of the beam-to-column connections could lead to up to 13 % material savings.