Molybdenum Fertilization Research Articles

New insights on molybdenum fertilization in common bean under no-tillage system: rates and application time to obtain high vigor seeds

Molybdenum (Mo) is a key element in the N fixing and assimilation processes in leguminous plants. This study aimed to evaluate the effects of Mo fertilization on the yield and seed vigor of the common bean. Molybdenum was applied at six rates (0, 20, 40, 80, 120 and 160 g ha−1) in field trials over 2 consecutive years (2015 and 2016). Foliar applications were performed during the phenological stages V4 (4th trifoliolate leaf unfolded) and R1 (early flowering). The SPAD index, height of first pod insertion, number of pods per plant, number of seeds per pod, weight of 100 seeds, yield and seed vigor were determined. Molybdenum affected the production of reproductive organs only in the second agricultural year. Application of Mo at T-V4 resulted in an increase in the number of pods per plant (11%), and at 120 g ha−1, there was an increase (41%) in the number of seeds per pod, but this did not result in a yield seed increase. Molybdenum applied at the T-V4 phase reduced electrical conductivity in the first year of the experiment at rates of 40 g ha−1 (17%) and 160 g ha−1 (13%). In the second agricultural year, Mo applied at the T-R1 phase reduced in 44% the electrical conductivity, as well as the application of 20 g ha−1 also at the T-R1 phase increased seed germination (28%), seedling length (8%), and length of the primary root (10%). Different responses to the Mo application time and dose between the experimental years suggest that common bean plants recycle Mo. Molybdenum applied in the first agricultural year may have satisfied the initial Mo demand of the common beans in the second year of crop due it's low Mo requirement. This study show that foliar Mo application is an important strategy to achieve sustainable production of higher quality seeds of common bean.

Theory and application of no-tillage reseeding in degraded grasslands

There are 400 million hectares of grasslands in China, accounting for about 40.7% of the Chinese territory, which provide fundamental ecological and economic benefits. However, more than 90% of this grassland ecosystem is threatened by degradation. This leads to the loss of biodiversity and a decrease in ecosystem functions, such as productivity, soil preservation and pest control. Currently, restoration of degraded grasslands is the most urgent task for grassland ecological management in China. No-tillage reseeding is one of the best practical technologies for grassland vegetation regeneration, because of the significant opportunity to improve productivity and plant diversity through establishing suitable species with minimum disturbance of the soil. Here we present a conceptual framework, which integrates plant-soil feedbacks, gap principles, and subclimax management models to guide our understanding of no-tillage reseeding. The key components of no-tillage reseeding in degraded grasslands consist of the selection of reseeding species, reseeding technology and reseeded grassland management. Native plants can affect the success of establishing species in revegetation due to competition for light, nutrients and water, and revegetation species with neutral or positive plant-soil feedbacks must be selected that can establish under these conditions. Our previous studies show that small light gaps can increase the performance of reseeded legumes, while larger light and soil gaps are needed for grass reseeding. Therefore, we predict that small gaps for legumes and large gaps for grasses will benefit these species respectively in no-tillage reseeding. According to the subclimax management model, suitable grassland management, for instance, mowing, grazing and fertilization, can maintain plant community composition, productivity and biodiversity, when the target species were successfully established. On the basis of this conceptual framework, no-tillage reseeding technology has been invented and applied in the semi-arid and semi-humid degraded grassland, and the plan for degraded grassland restoration nation-wide is proposed. We invented the “T” and “V” slot opener, which will result in minimal disturbance and help to maintain soil moisture after no-tillage reseeding. Given that plants differ in their ability to use soil nutrients; phosphorus, boron and molybdenum fertilization is recommended for legume reseeding and maintenance, while nitrogen fertilization can be applied to improve the performance of grasses. In summary, our study presents the scientific hypotheses and a framework for no-tillage reseeding technology, which will advance our understanding of successful grassland restoration and play an important role in sustainable grassland development in China.

Эффективность применения некорневых подкормок молибденовым и фосфорнокалийным удобрениями при капельном поливе на посевах лущильной фасоли

Эффективность применения некорневых подкормок молибденовым и фосфорнокалийным удобрениями при капельном поливе на посевах лущильной фасоли

Effects of spraying boron and molybdenum fertilizers on productivity and nutritional quality of alfalfa under drip irrigation

Effects of spraying boron and molybdenum fertilizers on productivity and nutritional quality of alfalfa under drip irrigation

Increase nutritional content and in vitro digestibility of forage legumes by adding molybdenum with foliar spray methods

Legumes as a source of protein is one of the most important nutrients for the ruminants. The experiment was carried out in the field of Forage Crop Laboratory of Padjadjaran University from october 2016 until January 2017, to study the effect of molybdenum fertilization on quality and in vitro digestibility on legumes. The design used was randomized block design with six treatment combinations consisting two levels of molybdenum (0 and 1.5 kg Mo ha-1) with foliar spray methods and three species of legumes (Pueraria phaseoloides, Calopogonium mucunoides and Centrosema pubescens) replicated four times. The results showed that the type of legume and molybdenum fertilizer had significant effect on crude protein content, ash content and in vitro digestibility but no significant effect on crude fiber content of forage legume.

Changes in Biological Nitrogen Fixation and Natural-Abundance N Isotopes of Sugarcane Under Molybdenum Fertilization

Molybdenum (Mo) can stimulate nitrogenase activity (NTA), increase biological nitrogen (N) fixation (BNF) and reduce natural-abundance N isotopes (δ15N). Our hypothesis is that this stimulus depends on genotype and N availability for sugarcane. The objective of this study was to evaluate the effect of the Mo fertilization on BNF, NTA, δ15N and the accumulation of N in different genotypes (RB867515 and RB92579) of sugarcane fertilized with N. The cultivars were subjected to two rates of N fertilization (without N fertilization and 60 kg ha−1) and two rates of Mo fertilization (without Mo fertilization and 200 g ha−1). NTA, δ15N and BNF were evaluated throughout the cycle of the sugarcane. Both leaf and root NTA was maximal at approximately 100 DAP. Mo fertilization stimulated NTA in RB867515 but reduced in RB92579. The bacteria associated with the sugarcane fixed atmospheric-N2 and the Mo fertilization stimulated this fixation, especially without N fertilization. Mo fertilization promoted greater N accumulation and should be encouraged for fertility management, especially in soils with low N content or as an alternative for reducing the use of N fertilizers in sugarcane.

The Response of Common Bean to Sulphur and Molybdenum Fertilization

The aim of the study was to assess the impact of sulphur and molybdenum fertilization on the yield and chemical composition of common bean seeds. A field experiment was conducted in southeastern Poland in 2012–2014. The scheme of the study included the following treatments: O-control, Mo-molybdenum (100 g·ha−1), SBS-sulphur before sowing (50 kg·ha−1), SFA-sulphur foliar application (50 kg·ha−1), Mo + SBS-molybdenum (100 g·ha−1) and sulphur before sowing (50 kg·ha−1), and Mo + SFA-molybdenum (100 g·ha−1) and sulphur foliar application (50 kg·ha−1). After harvesting, the following determinations were made in bean seeds: content of nitrogen, sulphur, phosphorus, potassium, calcium, magnesium, methionine, and cysteine. Application of Mo increased seed yield and protein and methionine content, as well as the content and uptake of P, Mg, and Ca in common bean seeds. Sulphur application had a positive effect on seed yield (13.6% increase) and protein content. Moreover, sulphur improved the biological value of protein by increasing the content of methionine, cysteine, and some macroelements. The most beneficial effects were obtained when both molybdenum and sulphur were used in fertilization. Considering the yield-producing effect and the impact on the biological quality of protein, sulphur fertilization should be included in the crop management for the common bean.

Application of Artificial Neural Networks for Multi-Criteria Yield Prediction of Winter Rapeseed

The aim of the work was to produce three independent, multi-criteria models for the prediction of winter rapeseed yield. Each of the models was constructed in such a way that the yield prediction can be carried out on three dates: April 15th, May 31st, and June 30th. For model building, artificial neural networks with multi-layer perceptron (MLP) topology were used, on the basis of meteorological data (temperature and precipitation) and information about mineral fertilisation. The data were collected from the years, 2008–2015, from 328 production fields located in Greater Poland, Poland. An assessment of the quality of forecasts produced based on neural models was verified by determination of forecast errors using RAE (relative approximation error), RMS (root mean square error), MAE (mean absolute error) error indicators, and MAPE (mean absolute percentage error). An important feature of the produced prediction models is the ability to realize the forecast in the current agrotechnical year on the basis of the current weather and fertiliser information. The lowest MAPE error values were obtained for the neural model WR15_04 (April 15th) based on the MLP network with structure 15:15-18-11-1:1, which reached 7.51%. Other models reached MAPE errors of 7.85% for model WR31_05 (May 31st) and 8.12% for model WR30_06 (June 30th). The performed sensitivity analysis gave information about the factors that have the greatest impact on winter rapeseed yields. The highest rank of 1 was obtained by two networks for the same independent variable in the form of the sum of precipitation within a period from September 1st to December 31st of the previous year. However, in model WR15_04, the highest rank obtained a feature in the form of a sum of molybdenum fertilization in the current year (MO_CY). The models of winter rapeseed yield produced in the work will be the basis for the construction of new forecasting tools, which may be an important element of precision agriculture and the main element of decision support systems.

Rhizobial Inoculation and Molybdenum Fertilization in Peanut Crops Grown in a No Tillage System After 20 Years of Pasture

ABSTRACT: Peanut (Arachis hypogea) is an important legume grain consumed by humans and utilized for effective nutrient cycling in a diverse cropping system. Areas that have been cultivated with perennial pasture for decades may have nutritional deficiencies and lack a sufficient population of atmospheric nitrogen-fixing bacteria. Molybdenum is an essential micronutrient that is part of the enzyme nitrogenase contained within symbiotic Bradyrhizobium bacteria, which are responsible for fixing nitrogen in legumes. Our objective was to evaluate the effects of application of Mo at different rates and a rhizobial inoculant on peanut growth characteristics. The experiment was conducted in the 2009/2010 growing season in a no-tillage cropping system following 20-year use as pasture [Urochloa brizantha (Syn. Brachiaria brizantha)]. The experimental design was a randomized complete block with four replicates. The main plots were characterized by peanut inoculation with Bradyrhizobium inoculant or without, and the split plots were characterized by different rates of molybdenum (0, 50, 100, and 200 g ha-1) applied to leaves in the form of ammonium molybdate. The nutritional status of plants, nodulation (number of nodules and nodule dry matter per plant), nitrogenase activity, and nitrogenase specific activity were evaluated at 45 and 64 days after emergence (DAE). The yield components and kernel yield were evaluated at the end of the growing season. Nitrogenase enzyme activity at 64 DAE approximately doubled, and the number of pods per plant was greater with inoculation than without, both of which led to greater yields of pods and kernels. In long-term pasture areas, inoculation and molybdenum fertilization greater than the currently recommended rate appear to be necessary to increase pod and kernel yield per hectare of peanut when managed under no-tillage.

Nitrate Reductase Activity and Nitrogen and Biomass Accumulation in Sugarcane under Molybdenum and Nitrogen Fertilization

ABSTRACT Prior research on sugarcane showed increment of productivity following molybdenum (Mo) fertilization and some indication of the potential role either on nitrate reductase activity (NRA). However, this increment can depend on sugarcane variety and nitrogen (N) fertilization. This study aimed to evaluate the effect of Mo fertilization on Mo uptake, on NRA, nitrate (NO3–-N), and ammonium (NH4+-N) contents in leaves and roots of sugarcane, and the accumulation of N and biomass across two sugarcane varieties (RB 867515 and RB [...]

Formation of seed productivity and sowing qualities of red clover seed depending on the effect of lime, mineral and water-soluble fertilizers

Aim. Development of effi cient fertilization of red clover seed sowings with mineral, lime and microfertilizers in order to optimize plant nutrition during their vegetation period to obtain stable seed yields with high sowing and yield qualities. Methods. fi eld, visual, measuring, weight, quantitative, method of a test sheaf, laboratory, mathematical-statistical. Results. The paper presents the results of optimizing the nutrition of red clover seed sowings on the basis of the rational application of quick-acting lime (Ca(OH) 2 – 0.5 of the rate by hydrolytic acidity, mineral (N 30 P 60 K 60 ) and water-soluble fertilizers, which ensured seed yield increase 1.8–2.0 times at the level of 0.35–0.40 t/ha. Conclusions. The most effective combination of the basic fertilization with mineral fertilizers (N 30 P 60 K 60 ) and lime fertilizers (Са(ОН) 2 ) at the rate of 0.5 by hydrolytic acidity applied under the cover crop using water-soluble fertilizer (plantafol – 1.0 kg/ha) and boric fertilizers (H 3 BO 4 – 0.8 kg/ha) at the shooting stage of the second cut of red clover and molybdenum fertilizers ((CNH 4 ) 2 MoO 4 – 0.3 kg/ha) in spring at the beginning of its regrowth.

Chickpea production and soil chemical attributes after phosphorus and molybdenum fertilization

ABSTRACT Chickpea is the third most cultivated legume in the world. In Brazil, cropping of this legume is recent and definitions of fertilizer management techniques are scarce. In this study, the objective was to evaluate chickpea (BRS Aleppo) production and soil chemical attributes with and without phosphorus fertilization and leaf-applied molybdenum. A randomized block experimental design was used, with four replications. A 2 x 5 factorial arrangement was used to evaluate production, consisting of two molybdenum fertilization management practices (with and without molybdenum) and five application rates of P2O5 (0, 60, 120, 180, and 240 kg ha-1) in the form of single superphosphate. Soil sampling for evaluation of soil chemical attributes in the crop row and between rows was also considered. Molybdenum fertilization brought about greater stem and aboveground dry matter. Phosphorus fertilization increased stem, leaf, and seed yield, yielding a maximum of 2.83 t ha-1 seed at the rate of 200 kg ha-1 P2O5. However, maximum agronomic efficiency (8.30) was observed with the addition of 150 kg ha-1 P. Soil in the crop row attained higher concentrations of P, K, H+Al, and P-rem and reduction in pH, Ca concentration, SB, T, and V compared to between rows. Phosphorus rates reduced soil pH and increased T and the P concentrations, though only in the plant row. Between the rows, no changes were observed in soil chemical attributes, indicating low mobility of P and the acidification capacity of superphosphate in alkaline soils.

Effect of Molybdenum Rate on Yield and Quality of Lettuce, Escarole, and Curly Endive Grown in a Floating System

Molybdenum (Mo) is required in enzymes involved in a number of different metabolic processes, and is crucial for the survival of plants and animals. The influence of nutrient solutions containing four levels of molybdenum (0, 0.5, 1.5, and 3.0 µmol/L) on growth, yield, and quality of lettuce, escarole, and curly endive grown in a hydroponic floating system was evaluated. Biometric, nutrient, and quality analyses were conducted to assess the response of each species to Mo. The results demonstrated that molybdenum is essential for harvesting marketable plants. Lettuce, escarole, and curly endive plants differed significantly in their response to molybdenum fertilization. The increase of Mo concentration in the nutrient solution was not harmful for plants and had no influence on yield and morphological traits of the leafy vegetables; however, it significantly affected some quality characteristics. Mo fertilization raised the nutritional quality by increasing ascorbic acid content up to 320.2, 139.0, and 102.1 mg kg−1 FW (fresh weight), and reducing nitrate content down to 1039.2, 1047.3, and 1181.2 mg kg−1 FW for lettuce, escarole, and curly endive, respectively. The addition of Mo in the nutrient solution increased the Mo content of plants up to 0.50, 4.02, and 2.68 μg g−1 FW for lettuce, escarole, and curly endive, respectively. Increasing Mo supply to lettuce, escarole, and curly endive up to 3.0 µmol L−1 could lead to a higher nutritional quality with no significant morphological alteration or yield loss.

Application of molybdenum fertilizer enhanced quality and production of alfalfa in northern China under non-irrigated conditions

ABSTRACTMolybdenum (Mo) is an essential micro-mineral nutrient for plant growth and development. As an important legume forage, alfalfa (Medicago sativa) is frequently planted in marginal lands of northern China where water scarcity and infertile soil are limiting factors for alfalfa production. Mo plays an important role in nitrogen (N) metabolism in general and N fixation in legume species in particular. To evaluate the role of Mo fertilizer in alfalfa quality and production in semi-arid areas of northern China, we conducted a three-year field experiment in Inner Mongolia from 2012 to 2014. Mo fertilizer at the doses of 0, 150 g hm−2, 300 g hm−2yr−1 was applied consecutively across the three years. Application of Mo at 300 g hm−2yr−1 significantly increased dry yield of alfalfa in 2014. Crude protein (CP) content of alfalfa without application of Mo exhibited a decline trend from 2012 to 2014. Application of Mo significantly enhanced total CP in 2012 and 2014, and delayed the reduction in total CP across the three years. In contrast to CP, Mo fertilizer had no significant effects on neutral detergent fiber and acid detergent fiber content. In addition, we found that nitrate reductase activity of alfalfa was increased by Mo fertilizer, which may account for the enhanced CP content in alfalfa. These findings highlight that application of Mo fertilizer can be an effective way to improve alfalfa production and quality in semi-arid areas in northern China.

Effects of micro-fertilizers foliar spray on the content of main effective components of Angelica dahurica

The zinc, boron and molybdenum were sprayed on the foliage of Angelica dahurica du-ring the vigorous growth period to explore the effects of the combination of zinc, boron and molybdenum fertilizers on the content of main effective components of A. dahurica, and to find out the optimum spraying amount. The results showed that the application of zinc, boron and molybdenum foliar spray was beneficial to increase the content of imperatorin and total cumarin, whereas had no effect on the content of the isoimperatorin. The effect of boron spray on the accumulation of imperatorin and total coumarin was the most significant, followed by the zinc and molybdenum. The interaction between boron and molybdenum had a negative cooperative effect on the content of imperatorin, while the interaction between zinc and molybdenum had a positivecooperative effect on the content of total coumarin. The contents of total coumarin reached over 0.7% respectively, with the complex fertilizer spray of zinc at 0.15-0.24 kg·hm-2, boron at 2.02-2.36 kg·hm-2 and molybdenum at 0.08-0.13 kg·hm-2. The contents of imperatorin reached over 0.2% respectively, with the complex fertilizer spray of zinc at 0.15-0.20 kg·hm-2, boron at 1.37-1.47 kg·hm-2 and molybdenum at 0.09-0.13 kg·hm-2. It was concluded that the application of zinc, boron and molybdenum foliar spray was beneficial to promote the quality of A. dahurica by enhancing its accumulation of coumarin.

Maize growth in response to Azospirillum brasilense, Rhizobium tropici, molybdenum and nitrogen

ABSTRACT The objective of this research was to evaluate the effect of Azospirillum brasilense, Rhizobium tropici, nitrogen (N) and molybdenum (Mo) fertilization on maize growth. The experiment was carried out in a greenhouse from October to November 2015, in a completely randomized design, in 2 x 2 x 2 x 5 factorial scheme, with 5 replicates, corresponding to the absence and presence of Azospirillum brasilense, Rhizobium tropici, N (30 kg ha-1) and five Mo doses (0, 7.5, 15.0, 22.5 and 30.0 g ha-1). The analyzed variables were: plant height, basal stem diameter, dry biomass of shoots, roots, total and N accumulated in the shoots. There was double or triple interaction between N fertilization, Azospirillum brasilense and Rhizobium tropici for the evaluated variables. However, isolated and/or combined effect of Mo was not observed. Seed inoculation with Azospirillum brasilense as well as their co-inoculation with Rhizobium tropici in the absence of N fertilization was efficient to increase plant growth. Soil N fertilization at sowing was less efficient in promoting plant growth than when it was combined with seed inoculation with Rhizobium tropici.

Effect of Rhizobium Inoculation Under Bio- And Mineral Fertilization and Their Interaction on Quality and Quantity of Soybean (Glycine Max L.)

Two field experiments were conducted at Sids Agricultural Research Station, A.R.C., Beni Swif Governorate to study the response of soybean variety Giza.111 to rhizobium inoculation combined with bio-fertilizer under mineral fertilization of nitrogen, phosphorus and molybdenum and their interactions on growth (plant height and dry weight/plant) , yields (seed and straw) , nutrients concentration and uptake and protein and oil percentages and yields. Four replicates in randomized complete block design were used in both experiments with the four factors of study. Results showed that plant height, dry weight/plant , seeds and straw yields and concentrations of nitrogen, phosphorus and molybdenum and uptake in seeds and straw as well as protein percentage and protein and oil yields were positively affected by rhizobium inoculation and 60, 13.1and 5kg/fed nitrogen , phosphorus and molybdate sodium except N and K % in seeds and straw , P % in seeds and Mo% in straw not affected by nitrogen , bio- and molybdenum fertilization . Moreover , oil percentage in seeds did not respond to the studied factors . The dual inoculation surpassed the single inoculation . As for the interaction, the statistical analysis showed that the high level of nitrogen had a antagonistic action on the effective effect of bacterial inoculation . On the other hand , added phosphorus had a synergistic effect on enhancing the positive effects of both bacterial inoculation and molybdenum on soybean growth and yields . In general , the treatment of rhizobium +bio- inoculation + 30 + 13.1 + 5 kg/fed nitrogen , phosphorus and molybdate sodium gave the highest quality and quantity of soybean .

Produtividade e composição mineral do feijão em resposta às adubações com molibdênio e níquel

RESUMO Níquel e molibdênio são micronutrientes essenciais que apresentam a capacidade de alterar o crescimento e o desenvolvimento do feijoeiro. O objetivo deste trabalho foi avaliar a produtividade, o crescimento e a composição mineral do feijão Ouro Vermelho em resposta às adubações com molibdênio (Mo) e níquel (Ni). O experimento foi conduzido a campo em Argissolo Acinzentado distrófico, no arranjo fatorial 5 x 2, referente a cinco doses de Ni (0; 15; 30; 45 e 60 g ha-1) e duas de Mo (0 e 80 g ha-1), no delineamento em blocos casualizados, com quatro repetições. A aplicação foliar de Ni e Mo foi feita aos 25 dias após a semeadura, por meio de um pulverizador manual. As características avaliadas foram: estande final, altura, produtividade, número de vagens por planta, número de sementes por vagem, massa de 100 sementes e os teores de N, P, K, Fe, Mo, e Ni. Verificou-se que a adubação molíbdica aumentou o teor de N na folha, reduziu o teor de Fe na semente e, em doses elevadas de Ni, reduziu o teor de Ni nas sementes. As doses de Ni, sem aplicação de Mo, aumentaram o teor de N na folha e, na presença do Mo, aumentaram o teor de Fe na semente. Houve aumento do número de grãos por vagem, com a aplicação de Ni, e da massa seca da parte aérea, com a adubação molíbdica. As adubações com 80 g ha-1 de Mo e 60 g ha-1 de Ni aumentaram em 12,71 e 27,5%, respectivamente, a produtividade do feijoeiro.

Is it possible to attain high-yielding common bean using molybdenum fertilizer instead of side-dressed nitrogen?

ABSTRACTWe evaluate the feasibility of using foliar-applied molybdenum, (Mo) instead of side-dressed nitrogen (N) in three experiments. In soils with native rhizobia, plants received 0, 30, 60, 90 or 120 kg N ha−1 with +Mo (80 g ha−1) or -Mo. N concentration in leaves (NCL) -Mo ranged from 35.1 to 42.5 g kg−1 and NCL +Mo from 40.3 to 49.2 g kg−1; yield -Mo ranged from 1560 to 3350 kg ha−1and yield +Mo from 2829 to 3567 kg ha−1. In two experiments, NCL or yield -Mo increased linearly or quadratically with increasing N rates, but NCL or yield +Mo did not. In one experiment, NCL increased linearly with increasing N rates and 16% with +Mo relative to –Mo, but yield was not affected significantly. Our results suggest that using Mo fertilizer instead of side-dressed N can allow common bean plants to meet crop demands for N to support yields as high as 3000 kg ha−1.

<b>Nitrogen and molybdenum fertilization and inoculation of common bean with <i>Rhizobium</i> spp. in two oxisols

The aim of this study was to evaluate the effects of foliar application of molybdenum and small dose of nitrogen at planting on common bean production when using seed inoculation with rhizobia. Two field experiments were conducted, applying a randomized block design and a factorial arrangement (3x2x2) + 1, with three replicates. Treatments consisted of inoculation with Rhizobium spp. (strain CIAT 899 T , strain UFLA 02-100, and uninoculated), foliar application of molybdenum (0 and 80 g ha -1 ), and fertilization with N-urea at planting (0 and 20 kg ha -1 ), with an additional N treatment of 40 applied at planting and 40 kg ha -1 applied as topdressing. The foliar application of molybdenum did not favor the nodulation, but its effect on yield was dependent on the chemical soil characteristics, providing highest yield in soil at Patos de Minas with pH more acid and with higher phosphorus and organic matter concentrations. Small dose of nitrogen applied at planting did not reduce nodulation of bean inoculated with rhizobia, but it can reduce the seeds emergence, thus negatively affecting yield. The native rhizobia promoted nodulation, the plant growth, shoot N accumulation and yield similar to the treatments that received inoculations of rhizobia strains.