Molecularly Imprinted Polymer Film Research Articles

Identification of Bioactive Compounds in Ginger Based on Molecularly Imprinted Polymer Quartz Crystal Microbalance Gas Sensor

Borneol, citral, and geraniol have been investigated as the major bioactive compound commonly found in ginger. In this work, a molecularly imprinted polymer (MIP) coated quartz crystal microbalance (QCM) sensor array has been employed for selective recognition of bioactive compounds in the ginger essential oil. In the experiment, the concentration of these bioactive compounds previously was measured using solid phase micro extraction gas chromatography-mass spectroscopy (SPME-GC/MS). Design of MIPs as the template of target molecules was created using polyacrylic acid (PAA) polymer matrix and three molecular targets (borneol, citral, and geraniol). An array of QCM sensor was prepared using four 9-Mhz AT-cut quartz crystal embedded between vacuum-deposited Au electrodes. For data recording, the headspace system flew the odorant of three varieties of ginger essential oil as positive control odorant and wild ginger essential oil as negative control odorant into the QCM sensor chamber. Then, mass loading in the MIP films caused frequency change of QCM sensor array due to odorant adsorption in a thin layer of MIP. Principal component analysis (PCA) and linear discriminant analysis (LDA) were applied to analyse the QCM response sensor. PCA score plot showed segregation of feature response of ginger essential oil with and without the molecular target in the coordinate of principal components. Meanwhile, LDA was able to discriminate training datasets of 80 ginger samples containing borneol, citral, and borneol with accuracy more than 92.50%.

"Gate Effect" in p-Synephrine Electrochemical Sensing with a Molecularly Imprinted Polymer and Redox Probes.

The "gate effect" mechanism for conductive molecularly imprinted polymer (MIP) film coated electrodes was investigated in detail. It was demonstrated that the decrease of the DPV signal for the Fe(CN)64-/Fe(CN)63- redox probe with the increase of the p-synephrine target analyte concentration in solution at the polythiophene MIP-film coated electrode did not originate from swelling or shrinking of the MIP film, as it was previously postulated, but from changes in the electrochemical process kinetics. The MIP-film coated electrode was examined with cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and surface plasmon resonance (SPR). The MIP-film thickness in the absence and in the presence of the p-synephrine analyte was examined with in situ AFM imaging. Moreover, it was demonstrated that doping of the MIP film was not affected by p-synephrine binding in MIP-film molecular cavities. It was concluded that the "gate effect" was most likely caused by changes in radical cation (polaron) mobility in the film.

A novel electrochemical sensor based on electropolymerized molecularly imprinted polymer for determination of luteolin

A novel molecularly imprinted polymer (MIP) electrochemical sensor was prepared and applied for highly selective and sensitive electrochemical determination of luteolin. The MIP film was electrodeposited on the indium-tin oxide (ITO) glass electrode surface through the electropolymerization of β-cyclodextrin (β-CD) as the functional monomer and luteolin as the template molecule. The MIP sensor displays a rapid electrochemical response, high sensitivity and specific binding of the template luteolin. Under the optimized experimental conditions, the linear response range for luteolin concentrations by the sensor is 5.0×10−8mol/L to 3.0×10−5mol/L and the detection limit is 2.4×10−8mol/L (S/N=3). The analytical performance of the proposed MIP sensor is evaluated for the detection of luteolin in the traditional medicine as a real sample with good accuracy and precision.

An impedimetric molecularly-imprinted biosensor for Interleukin-1β determination, prepared by in-situ electropolymerization on carbon screen-printed electrodes

This work reports the first electrochemical molecularly imprinted polymer (MIP) sensor for Interleukin-1beta (IL-1β) detection, based on modified commercial screen-printed carbon electrode (SPCE) was successfully demonstrated. For this purpose, the carbon support was modified with a PEDOT/4-aminothiophenol layer prior to the MIP film to enhance sensitivity and signal stability. The MIP layer was constructed on top of this by electropolymerization of Eriochrome black T (EBT) in the presence of IL-1β. The several steps of the biosensor assembly was followed by Raman spectroscopy and electroanalytical techniques.Using electrochemical impedance spectroscopy (EIS), a linear response in the range of 60 pM to 600 nM, with a LOD of 1.5 pM with (S/N = 3) was obtained in neutral PBS. Selectivity tests of the MIP biosensor made in spiked synthetic serum samples as well as against other structurally related (Myoglobin, of similar shape and size) or competing compounds (Immunoglobulin G, also present in the human serum) confirmed the good selectivity of the biosensor.Overall, the biosensor described herein has the potential to provide a simple and quick way for on-site screening of IL-1β, with low sample/reagent consumption and enabling direct serum analysis, which constitutes a valuable alternative to other conventional methods.

‘Gate effect’ in molecularly imprinted polymers: the current state of understanding

Abstract The so-called ‘gate effect’ is frequently used in electrochemical signal transduction for chemosensing developed using recognition units based on molecularly imprinted polymers (MIPs). A vast majority of different reported MIP electroanalytical systems makes the gate effect definition difficult to be established precisely, thus resulting in ambiguity and complexity. In the MIP literature, the gate effect is predominantly considered as an electrode–electrolyte phenomenon, which depends on the MIP film nature. However, most of the reports do not describe or confirm the origin of this effect. The present review critically evaluates representative original articles on the subject to increase understanding the origin of the gate effect operation in MIP-based electrochemical chemosensors.

A novel sensitive and selective electrochemical sensor based on integration of molecularly imprinted with hollow silver nanospheres for determination of carbamazepine

An electrochemical sensor based on hollow silver nanospheres (hAgNS) and molecularly imprinted polymer (MIP) was developed for drug analysis by using carbamazepine (CBZ) as a model analyte. hAgNS, serving as a support material for MIP immobilization, possesses large accessible surface area with superb electric conductivity, while electrochemically synthesized MIP film warrants improved selectivity for CBZ. Quantification based on the “MIP/gate effect” was performed by employing Fe(CN)63−/4− as the probe to indicate the current intensity. The sensor materials were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimal conditions, a linear relationship of the electrical signal versus the concentration of CBZ was obtained in the range from 8.0 × 10−11 to 6.0 × 10−8 M with a detection limit of 3.2 × 10−11 M (S/N = 3). Moreover, the prepared sensor exhibited specific detection of CBZ over its structural analogues and interferents, and the established electrochemical method was validated using the standard method-high performance liquid chromatography (HPLC). Eventually, rapid and accurate determination of CBZ in rat serum and cell samples was carried out after easy sample pretreatment.

Selective PQQPFPQQ Gluten Epitope Chemical Sensor with a Molecularly Imprinted Polymer Recognition Unit and an Extended-Gate Field-Effect Transistor Transduction Unit.

A molecularly imprinted polymer (MIP) recognition system was devised for selective determination of an immunogenic gluten octamer epitope, PQQPFPQQ. For that, a thin MIP film was devised, guided by density functional theory calculations, and then synthesized to become the chemosensor recognition unit. Bis(bithiophene)-based cross-linking and functional monomers were used for this synthesis. An extended-gate field-effect transistor (EG-FET) was used as the transduction unit. The EG-FET gate surface was coated with the PQQPFPQQ-templated MIP film, by electropolymerization, to result in a complete chemosensor. X-ray photoelectron spectroscopy analysis confirmed the presence of the PQQPFPQQ epitope, and its removal from the MIP film. The chemosensor selectively discriminated between the octamer analyte and another peptide of the same number of amino acids but with two of them mismatched (PQQQFPPQ). The chemosensor was validated with respect to both the PQQPFPQQ analyte and a real gluten extract from semolina flour. It was capable to determine PQQPFPQQ in the concentration range of 0.5-45 ppm with the limit of detection (LOD) = 0.11 ppm. Moreover, it was capable of determining gluten in real samples in the concentration range of 4-25 ppm with LOD = 4 ppm, which is a value sufficient for discriminating between gluten-free and non-gluten-free food products. The gluten content in semolina flour determined with the chemosensor well correlated with that determined with a commercial ELISA gluten kit. The Langmuir, Freundlich, and Langmuir-Freundlich isotherms were fitted to the epitope sorption data. The sorption parameters determined from these isotherms indicated that the imprinted cavities were quite homogeneous and that the epitope analyte was chemisorbed in them.

Novel molecularly imprinted polymer (MIP) multiple sensors for endogenous redox couples determination and their applications in lung cancer diagnosis

Multiplex electrochemical sensors for amperometric detection of glutathione disulfide (GSSG), glutathione (GSH), cysteine (Cys), cystine (Cyss), β-nicotinamide adenine dinucleotide phosphate (NADP+) and coenzyme II reduced tetrasodium salt (NADPH) were developed, in which analysis of Cyss, NADP+ and NADPH are the first report using this sensing system. Specificity of these sensors were ensured by a layer of molecularly imprinted polymer (MIP) which was electropolymerized in situ with the analytes as template. All the sensors were tested with standard buffers and mouse blood samples, showing satisfactory performance towards the corresponding analytes. Dynamic concentration for the six analytes was in the range of 10−11–10−8 mol/L with the detection limit down to 20 pmol/L. In addition, artificially synthesized MIP film on the electrodes allowed for good selectivity and stability. Real blood sample measurement proved that the sensors owned decent accuracy with recovery value ranging from 92%~112%. More importantly, blood samples from lung cancer patients and healthy donors were assayed by using the proposed sensors. Redox potentials (Ehc) were calculated based on the contents of these endogenic substances, which were utilized to reflect the health status of human body and help diagnose lung cancer. The levels of GSH, NADPH and the absolute value of Ehc(GSH/GSSG) in patients with lung cancer are significantly lower (P < 0.01) than those in healthy people, while the contents of GSSG (P < 0.01) are higher. The blood test results suggested that the content of GSH, NADPH, NADP+ and Ehc(GSH/GSSG) might serve as biomarkers for lung cancer prediagnosis. These novel sensors for liquid biospy of cancer have cost–benefit and scalability advantage over current techniques, potentially enabling broader clinical access and efficient population screening.

Promoting bioanalytical concepts in genetics: A TATA box molecularly imprinted polymer as a small isolated fragment of the DNA damage repairing system

We demonstrate that a new, stable, artificial TATA (T — thymine, A — adenine) box is recognized by amino acids recognizing the natural TATA box. Here, the former mimicked, as a minimal motif, oligodeoxyribonucleotide interactions with amino acids of proteins involved in repairing of damaged dsDNA. By electropolymerization, we molecularly imprinted non-labeled 5′-TATAAA-3′ via Watson-Crick nucleobase pairing, thus synthesizing, in a one-step procedure, the hexakis[bis(2,2′-bithien-5-yl)] TTTATA and simultaneously hybridizing it with the 5′-TATAAA-3′ template. That is, a stable dsDNA analog having a controlled sequence of nucleobases was formed in the molecularly imprinted polymer (MIP). The 5′-TATAAA-3′ was by the X-ray photoelectron spectroscopy (XPS) depth profiling found to be homogeneously distributed both in the bulk of the MIP film and on its surface. The 5′-TATAAA-3′ concentration in the 2.8(±0.2)-nm relative surface area, ~140-nm thick MIP film was 2.1 mM. The MIP served as a matrix of an artificial TATA box with the TATAAA-promoter sequence. We comprehensively characterized this artificial DNA hybrid by the polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS) and X-ray photoelectron spectroscopy (XPS). Further, we examined interactions of DNA repairing TATA binding protein (TBP) amino acids with the artificial TATA box prepared. That is, molecules of l-phenylalanine aromatic amino acid were presumably engaged in stacking interactions with nucleobase steps of this artificial TATA box. The nitrogen-to‑phosphorus atomic % ratio on the surface of the MIP-(5′-TATAAA-3′) film increased by ~1.6 times after film immersing in the l-glutamic acid solution, as determined using the XPS depth profiling. Furthermore, l-lysine and l-serine preferentially interacted with the phosphate moiety of 5′-TATAAA-3′. We monitored amino acids interactions with the artificial TATA box using real-time piezoelectric microgravimetry at a quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) spectroscopy under flow injection analysis (FIA) conditions.

Facile Fabrication of Surface-Imprinted Macroporous Films for Chemosensing of Human Chorionic Gonadotropin Hormone.

We present an improved approach for the preparation of highly selective and homogeneous molecular cavities in molecularly imprinted polymers (MIPs) via the combination of surface imprinting and semi-covalent imprinting. Toward that, first, a colloidal crystal mold was prepared via the Langmuir-Blodgett(LB) technique. Then, human chorionic gonadotropin (hCG) template protein was immobilized on the colloidal crystal mold. Later, hCG derivatization with electroactive functional monomers via amide chemistry was performed. In a final step, optimized potentiostatic polymerization of 2,3'-bithiophene enabled depositing an MIP film as the macroporous structure. This synergistic strategy resulted in the formation of molecularly imprinted cavities exclusively on the internal surface of the macropores, which were accessible after dissolution of silica molds. The recognition of hCG by the macroporous MIP film was transduced with the help of electric transducers, namely, extended-gate field-effect transistors (EG-FET) and capacitive impedimetry (CI). These readout strategies offered the ability to create chemosensors for the label-free determination of the hCG hormone. Other than the simple confirmation of pregnancy, hCG assay is a common tool for the diagnosis and follow-up of ectopic pregnancy or trophoblast tumors. Concentration measurements with these EG-FET and CI-based devices allowed real-time measurements of hCG in the range of 0.8-50 and 0.17-2.0 fM, respectively, in 10 mM carbonate buffer (pH = 10). Moreover, the selectivity of chemosensors with respect to protein interferences was very high.

Preparation of Molecularly Imprinted Composites Initiated by Hemin/Graphene Hybrid Nanosheets and Its Application in Detection of Sulfamethoxazole.

Molecularly imprinted polymers (MIPs) exhibit high selectivity resulting from imprinted cavities and superior performance from functional materials, which have attracted much attention in many fields. However, the combination of MIPs film and functional materials is a great challenge. In this study, hemin/graphene hybrid nanosheets (H-GNs) were used to initiate the imprinted polymerization by catalyzing the generation of free radicals. Thus, MIPs using sulfamethoxazole as the template was directly prepared on the surface of H-GNs without any film modification. Most importantly, the template could be absorbed on the H-GNs to enhance the number of imprinted sites per unit surface area, which could improve the selectivity of MIPs film. Thus, the composites could exhibit high adsorption capacity (29.4 mg/g), imprinting factor (4.2) and excellent conductivity, which were modified on the surface of electrode for rapid, selective and sensitive detection of sulfamethoxazole in food and serum samples. The linear range was changed from 5 μg/kg to 1 mg/g and the limit of detection was 1.2 μg/kg. This sensor was free from interference caused by analogues of sulfamethoxazole, which provides a novel insight for the preparation of MIPs-based sensor and its application in food safety monitoring and human exposure study.

Determination of Diuron by Using Electrochemical Sensor Based on Molecularly Imprinted Polymer Film

In this study, pencil graphite electrodes were modified by electropolymerization of p-phenylenediamine polymer imprinted with diuron to develop a sensor for diuron detection. Polymer growth and template leach conditions were investigated for determining optimal settings in developing the sensor. Under optimal working conditions, the differential pulse voltammetric current response of diuron oxidation was linear in the range of 10–500 μM (R2: 0.9875) with a detection limit of 43.43 μM (S/N: 10). The molecularly imprinted polymer modified electrode showed selectivity and sensitivity for diuron compared to its structural analogues. The developed sensor was applied to the detection of diuron in water samples.

Molecularly imprinted polymer-based electrochemical sensors for biopolymers

Abstract Electrochemical synthesis and signal generation dominate among the almost 1200 articles published annually on protein-imprinted polymers. Such polymers can be easily prepared directly on the electrode surface, and the polymer thickness can be precisely adjusted to the size of the target to enable its free exchange. In this architecture, the molecularly imprinted polymer (MIP) layer represents only one ‘separation plate’; thus, the selectivity does not reach the values of ‘bulk’ measurements. The binding of target proteins can be detected straightforwardly by their modulating effect on the diffusional permeability of a redox marker through the thin MIP films. However, this generates an ‘overall apparent’ signal, which may include nonspecific interactions in the polymer layer and at the electrode surface. Certain targets, such as enzymes or redox active proteins, enables a more specific direct quantification of their binding to MIPs by in situ determination of the enzyme activity or direct electron transfer, respectively.

Molecularly-imprinted chloramphenicol sensor with laser-induced graphene electrodes

Graphene has emerged as a novel material with enhanced electrical and structural properties that can be used for a multitude of applications from super-capacitors to biosensors. In this context, an ultra-sensitive biosensor was developed using a low-cost, simple and mask-free method based on laser-induced graphene technique for electrodes patterning. The graphene was produced on a polyimide substrate, showing a porous multi-layer structure with a resistivity of 102.4 ± 7.3 Ω/square. The biosensor was designed as a 3-electrode system. Auxiliary and working electrodes were made of graphene by laser patterning and the reference electrode was handmade by casting a silver ink. A molecularly-imprinted polymer (MIP) was produced at the working electrode by direct electropolymerization of eriochrome black T (EBT). As proof-of-concept, the MIP film was tailored for chloramphenicol (CAP), a common contaminant in aquaculture.The resulting device was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy readings against a redox standard probe. The limit of detection (LOD) was 0.62 nM and the linear response ranged from 1 nM to 10 mM. These analytical features were better than those produced by assembling the same biorecognition element on commercial graphene- and carbon-based screen-printed electrodes.Overall, the simplicity and quickness of the laser-induced graphene technique, along with the better analytical features obtained with the graphene-based electrodes, shows the potential to become a commercial approach for on-site sensing.

Hybrid molecularly imprinted polymer for amoxicillin detection

The potential adverse effects of the environmental presence of antibiotics on the ecosystem demands the development of new methods suitable for accurate detection of these micropollutants in various aquatic media. An analytical method exploiting the synergistic effect of a label-free sensing platform combined with a molecularly imprinted polymer (MIP) as robust recognition element could represent an efficient tool for the real-time monitoring of antibiotics. In this work, a hybrid organic-inorganic MIP film (AMO-MIP) selective towards amoxicillin (AMO) was synthesized and integrated with a surface plasmon resonance (SPR) sensor. The film was prepared by sol-gel using methacrylamide (MAAM) as organic functional monomer, tetraethoxysilane (TEOS) as inorganic precursor, and vinyltrimethoxysilane (VTMOS) as coupling agent. The AMO-MIP film characterized with the SPR system demonstrated about 16 times higher binding capacity to AMO than corresponding reference non-imprinted polymer (NIP). AMO-MIP-modified SPR sensors could detect AMO with LoD down to 73 pM and discriminate AMO among structurally similar molecules both in buffer and in tap water. Good reproducibility was achieved for several rebinding-regeneration cycles. The sensor could be stored at room temperature for up to 6 months without losing stability.

Selective recognition of Histidine enantiomers using novel molecularly imprinted organic transistor sensor

Abstract l -Histidine (L-His) is an essential bioactive amino acid, playing a significant role in several biological processes. Therefore, the accurate determination of L-His is of great significance to human health. In this work, a strategy was developed for the chiral detection of L-His using an organic electrochemical transistor (OECT) sensor based on molecularly imprinted polymer (MIP). MIP films were electrodeposited on OECT gate platforms. MIP films designed for specifically recognizing His combined with the amplified function of an OECT yielded highly sensitive and selective OECT biosensor. The properties of the resulting biosensor were characterized by various analytical methods. The results indicated that changes in channel current was proportional to D-His and L-His concentrations from 100 nM to 10 μM, with the detection limits of 10 nM and 100 nM (S/N > 3), respectively. Furthermore, the highly selective MIP film allowed to differentiate L-His from D-His or D-His from L-His. In terms of selectivity, the tests suggested apparent differences in channel current changes between L-His and D-His and interferences. The applicability of the biosensor for L-His detection in human urine demonstrated this sensor will be ideal transducers for disposable applications in the future.

A screen-printed carbon electrode modified with gold nanoparticles, poly(3,4-ethylenedioxythiophene), poly(styrene sulfonate) and a molecular imprint for voltammetric determination of nitrofurantoin.

A molecularly imprinted polymer (MIP) and a nanocomposite prepared from gold nanoparticles (AuNP) and poly(3,4-ethylenedioxythiophene)/poly(styrene sulfonate) (PEDOT:PSS) were deposited on a screen-printed carbon electrode (SPCE). The nanocomposite was prepared by one-pot simultaneous in-situ formation of AuNPs and PEDOT:PSS and was then inkjet-coated onto the SPCE. The MIP film was subsequently placed on the modified SPCE by co-electrodeposition of o-phenylenediamine and resorcinol in the presence of the antibiotic nitrofurantoin (NFT). Using differential pulse voltammetry (DPV), response at the potential of ~ 0.1V (vs. Ag/AgCl) is linear in 1nM to 1000nM NFT concentration range, with a remarkably low detection limit (at S/N = 3) of 0.1nM. This is two orders of magnitude lower than that of the control MIP sensor without the nanocomposite interlayer, thus showing the beneficial effect of AuNP-PEDOT:PSS. The electrode is highly reproducible (relative standard deviation 3.1% for n = 6) and selective over structurally related molecules. It can be re-used for at least ten times and was found to be stable for at least 45days. It was successfully applied to the determination of NFT in (spiked) feed matrices and gave good recoveries. Graphical abstract Schematic representation of a voltammetric sensor for the determination of nitrofurantoin. The sensor is based on a screen-printed carbon electrode (SPCE) modified with an inkjet-printed gold nanoparticles-poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) nanocomposite and a molecularly imprinted polymer.

Utilization of an environmentally-friendly monomer for an efficient and sustainable adrenaline imprinted electrochemical sensor using graphene

Generally, the toxic and mutagenic functional monomers are employed to synthesize the MIP films. This work demonstrates the fabrication of molecular imprinted polymer (MIP) based electrochemical sensor for adrenaline using a green functional monomer, nicotinamide. The adrenaline imprinted film was electropolymerized over reduced graphene oxide (rGO) modified glassy carbon electrode (GCE) by cyclic voltammetry (CV). The as-synthesized sensors were characterized by SEM. The electrochemical analysis showed that the graphene-modified electrode exhibited excellent electrocatalytic activity toward adrenaline and the over-potential of adrenaline decreased significantly as compared to bare GCE which can be attributed to graphene's unique physical and chemical properties such as subtle electronic characteristics, attractive π–π interaction, and strong adsorptive capability of MIP film. The thickness of MIP was estimated to be 1.97 nm by coulometric analysis. This electrochemical sensor displays high selectivity owing to specific imprinted cavities for adrenaline and worked well over a wide linear concertation range of adrenaline between 0.015 μM and 40 μM with a detection limit (LOD) of 3 nM. The sensor offered good reproducibility (Relative standard deviation of 3.44%) and retained 94.6% of its initial response for over 40 days. Finally, excellent recoveries from 96.0% to 100.7% were obtained for two different samples of urine and adrenaline ampoules. Thus, this work holds great promise with numerous advantages including easy fabrication, high sensitivity and selectivity, high throughput and satisfactory reproducibility for adrenaline.

Photonic Molecularly Imprinted Polymer Film for the Detection of Testosterone in Aqueous Samples.

The detection of testosterone in aqueous solutions is a difficult task due to the low concentration levels that are relevant in environmental and physiological samples. Current analytical methods are expensive and/or complex. To address this issue, we fabricated a molecularly imprinted polymer (MIP) photonic film for the detection of testosterone in water. The films were obtained using colloidal crystals as templates for the pore morphology. Monodispersed silica particles with an average diameter 330 nm were used to obtain the colloidal crystal by vertical deposition. A solution of acrylic acid with testosterone as the imprinted template was infiltrated in the colloidal crystal and polymerized via bulk polymerization; the particles were then removed by acid etching and the testosterone eluted by a suitable solvent. The material was characterized by FTIR, swelling experiments and microscopy; MIPs were investigated by equilibrium rebinding, kinetics and reuse experiments. The results showed that the MIPs exhibited selectivity to the template, a 30-min equilibration time and stability after at least six cycles of use and regeneration. After incubation, the reflectance spectra of the films showed a shift of the Bragg diffraction peak that correlated with testosterone concentration in the 5–100 ppb range.

A Surface Plasmon Resonance-Based Optical Fiber Probe Fabricated with Electropolymerized Molecular Imprinting Film for Melamine Detection.

Molecularly imprinted polymer (MIP) films prepared by bulk polymerization suffer from numerous deficiencies, including poor mass transfer ability and difficulty in controlling reaction rate and film thickness, which usually result in poor repeatability. However, polymer film synthesized by electropolymerization methods benefit from high reproducibility, simplicity and rapidity of preparation. In the present study, an Au film served as the refractive index-sensitive metal film to couple with the light leaked out from optical fiber core and the electrode for electropolymerizing MIP film simultaneously. The manufactured probe exhibited satisfactory sensitivity and specificity. Furthermore, the surface morphology and functional groups of the synthesized MIP film were characterized by Atomic Force Microscopy (AFM) and Fourier transform infrared microspectroscopy (FTIR) for further insights into the adsorption and desorption processes. Given the low cost, label-free test, simple preparation process and fast response, this method has a potential application to monitor substances in complicated real samples for out-of-lab test in the future.