Molecular Depletion Research Articles

Relating dust, gas, and the rate of star formation in M 31

We derive distributions of dust temperature and dust opacity across M31 at 45" resolution using the Spitzer data. With the opacity map and a standard dust model we de-redden the Ha emission yielding the first de-reddened Ha map of M31. We compare the emissions from dust, Ha, HI and H2 by means of radial distributions, pixel-to-pixel correlations and wavelet cross-correlations. The dust temperature steeply decreases from 30K near the center to 15K at large radii. The mean dust optical depth at the Ha wavelength along the line of sight is about 0.7. The radial decrease of the dust-to-gas ratio is similar to that of the oxygen abundance. On scales<2kpc, cold dust emission is best correlated with that of neutral gas and warm dust emission with that of ionized gas. Ha emission is slightly better correlated with emission at 70um than at 24um. In the area 6kpc<R< 17kpc, the total SFR is ~0.3Msun/yr. The Kennicutt-Schmidt law between SFR and total gas has a power-law index of 1.30+-0.05 in the radial range of R=7-11kpc increasing by about 0.3 for R=11-13kpc. The lack of H2 in the central region could be related to the lack of HI and the low opacity/high temperature of the dust. Since neither SFR nor SFE is well correlated with the surface density of H2 or total gas, other factors than gas density must play an important role in the formation of massive stars in M31. The molecular depletion time scale of 1.1 Gyr indicates that M31 is about three times less efficient in forming young massive stars than M33.

Molecular depletion of descending serotonin unmasks its novel facilitatory role in the development of persistent pain.

Recent studies indicate that persistent pain after tissue or nerve injury is accompanied by an enhanced net descending facilitatory drive that contributes to an amplification and spread of pain. Although 5-HT-containing neurons in the rostral ventromedial medulla (RVM) provide the major descending serotonergic projection to the spinal cord, it is not clear whether the neurotransmitter 5-HT itself released from RVM-spinal neurons contributes to descending pain modulation. In the present study, we determined the role of the descending 5-HT in rat nocifensive behaviors after persistent pain by selectively depleting functional phenotypes of 5-HT in RVM neurons with regional shRNA interference (RNAi) of tryptophan hydroxylase-2 (Tph-2), the rate-limiting enzyme in the synthesis of neuronal 5-HT. Compared to negative control shRNA, Tph-2 shRNA induced significantly prolonged downregulation of Tph-2 in the RVM and 5-HT in spinal dorsal horn. The 5-HT-depleted rats showed normal pain sensitivity in responses to acute noxious stimulation. However, the same RNAi treatment attenuated formalin-induced spontaneous nocifensive responses and tissue or nerve injury-induced allodynia and hyperalgesia. Furthermore, in control shRNA-treated animals, intra-RVM microinjection of brain-derived neurotrophic factor produced a reversible hyperalgesia, which was completely prevented by Tph-2 RNAi pretreatment. Descending inhibition induced by intra-RVM electrical stimulation, but not microinjection of the mu- or kappa-opioid receptor agonists in control shRNA-treated animals was eliminated in 5-HT-depleted rats. These results indicate that the descending 5-HT from the RVM is an important contributor to pain facilitation during the development of persistent pain, and may not mediate opioid-induced descending inhibition in acute pain.

Measurement of Molecular Diffusion Based on Optoelectrofluidic Fluorescence Microscopy

This technical note reports a method for measuring the diffusion coefficient of molecules based on an optoelectrofluidic platform. Optoelectrofluidic fluorescence microscopy, which is constructed with an optoelectrofluidic device and a conventional fluorescence microscope, is a useful tool for controlling and detecting local molecular concentration in a fluid with a single light source. When we project a light for fluorescence excitation and apply an ac signal of a few hundred Hertz frequency around 100 Hz to the optoelectrofluidic device, a sudden decay of molecular concentration occurs within the illuminated area due to several mechanisms, including optically induced ac electroosmosis, electrostatic interaction forces among the polarized molecules, and interactions among the molecules and between the molecules and the electric double layer. After the applied voltage was turned off, the dispersed molecules diffuse into the molecular depletion area and the fluorescence signal is recovered. On the basis of these phenomena, we successfully measured the diffusion coefficient of various dextran molecules. A statistical analysis to determine the significance of our experimental values compared to the previously reported values measured using fluorescence recovery after photobleaching technology was also performed. This new technique demonstrates the first analytical measurement of diffusion based on the optoelectrofluidic platform and can be a useful tool for measuring the mobility of molecules in a simple and easy way.

THE MOLECULAR EMISSION OF THE IRRADIATED STAR-FORMING CORE AHEAD OF HH 80N

We present a Berkeley-Illinois-Maryland Association (BIMA) Array molecular survey of the star forming core ahead of HH 80N, the optically obscured northern counterpart of the Herbig-Haro objects HH 80/81. Continuum emission at 1.4 mm and 8 micron is detected at the center of the core, which confirms the presence of an embedded very young stellar object in the core. All detected molecular species arise in a ring-like structure, which is most clearly traced by CS(2-1) emission. This molecular ring suggests that strong molecular depletion occurs in the inner part of the core (at a radius of ~0.1 pc and densities higher than ~5 x 10^4 cm^{-3}). Despite of the overall morphology and kinematic similarity between the different species, there is significant molecular differentiation along the ring-like structure. The analysis of the chemistry along the core shows that part of this differentiation may be caused by the UV irradiation of the nearby HH 80N object, that illuminates the part of the core facing HH 80N, which results in an abundance enhancement of some of the detected species.

Langmuir−Blodgett Film of Hydrophobin Protein from Pleurotus ostreatus at the Air−Water Interface

We present results concerning the formation of Langmuir-Blodgett (LB) films of a class I hydrophobin from Pleurotus ostreatus at the air-water interface, and their structure as Langmuir-Blodgett (LB) films when deposited on silicon substrates. LB films of the hydrophobin were investigated by atomic force microscopy (AFM). We observed that the compressed film at the air-water interface exhibits a molecular depletion even at low surface pressure. In order to estimate the surface molecular concentration, we fit the experimental isotherm with Volmer's equation describing the equation of state for molecular monolayers. We found that about (1)/ 10 of the molecules contribute to the surface film formation. When transferred on silicon substrates, compact and uniform monomolecular layers about 2.5 nm thick, comparable to a typical molecular size, were observed. The monolayers coexist with protein aggregates, under the typical rodlet form with a uniform thickness of about 5.0 nm. The observed rodlets appear to be a hydrophilic bilayer and can then be responsible for the surface molecular depletion.

Molecular Line Observations of the Small Protostellar Group L1251B

We present molecular line observations of L1251B, a small group of pre- and protostellar objects, and its immediate environment in the dense C18O core L1251E. These data are complementary to near-infrared, submillimeter and millimeter continuum observations reported by Lee et al. (2006, ApJ, 648, 491; Paper I). The single-dish data of L1251B described here show very complex kinematics including infall, rotation and outflow motions, and the interferometer data reveal these in greater detail. Interferometer data of N2H+ 1-0 suggest a very rapidly rotating flattened envelope between two young stellar objects, IRS1 and IRS2. Also, interferometer data of CO 2-1 resolve the outflow associated with L1251B seen in single-dish maps into a few narrow and compact components. Furthermore, the high resolution data support recent theoretical studies of molecular depletions and enhancements that accompany the formation of protostars within dense cores. Beyond L1251B, single-dish data are also presented of a dense core located ~150 to the east that, in Paper I, was detected at 850 micron but has no associated point sources at near- and mid-infrared wavelengths. The relative brightness between molecules, which have different chemical timescales, suggests it is less chemically evolved than L1251B. This core may be a site for future star formation, however, since line profiles of HCO+, CS, and HCN show asymmetry with a stronger blue peak, which is interpreted as an infall signature.

Desorption from interstellar ices

The desorption of molecular species from ice mantles back into the gas phase in molecular clouds results from a variety of very poorly understood processes. We have investigated three mechanisms: desorption resulting from H2 formation on grains, direct cosmic ray heating and cosmic ray-induced photodesorption. Whilst qualitative differences exist between these processes (essentially deriving from the assumptions concerning the species selectivity of the desorption and the assumed threshold adsorption energies, Et), all the three processes are found to be potentially very significant in dark cloud conditions. It is therefore important that all three mechanisms should be considered in studies of molecular clouds in which freeze-out and desorption are believed to be important. Employing a chemical model of a typical static molecular core and using likely estimates for the quantum yields of the three processes, we find that desorption by H2 formation probably dominates over the other two mechanisms. However, the physics of the desorption processes and the nature of the dust grains and ice mantles are very poorly constrained. We therefore conclude that the best approach is to set empirical constraints on the desorption, based on observed molecular depletions – rather than try to establish the desorption efficiencies from purely theoretical considerations. Applying this method to one such object (L16 89B) yields upper limits to the desorption efficiencies that are consistent with our understanding of these mechanisms.

The structure of the cometary globule CG 12: a high-latitude star-forming region

The structure of the high galactic latitude Cometary Globule 12 (CG 12) has been investigated by means of radio molecular line observations. Detailed, high signal to noise ratio maps in C18O (1-0), C18O (2-1) and molecules tracing high density gas, CS (3-2), DCO+ (2-1) and H13CO+ (1-0), are presented. The C18O line emission is distributed in a 10' long North-South elongated lane with two strong maxima, CG12 N(orth) and CG12 S(outh). In CG12 S the high density tracers delineate a compact core, DCO+ core, which is offset by 15 from the C18O maximum. The observed strong C18O emission traces the surface of the DCO+ core or a separate, adjacent cloud component. The emission in high density tracers is weak in CG12 N and especially the H13CO+, DCO+ and N2H+ lines are +0.5 km/s offset in velocity with respect to the C18O lines. Evidence is presented that the molecular gas is highly depleted. The observed strong C18O emission towards CG12 N originates in the envelope of this depleted cloud component or in a separate entity seen in the same line of sight. The C18O lines in CG 12 were analyzed using Positive Matrix Factorization, PMF. The shape and the spatial distribution of the individual PMF factors fitted separately to the C18O (1-0) and (2-1) transitions were consistent with each other. The results indicate a complex velocity and line excitation structure in the cloud. Besides separate cloud velocity components the C18O line shapes and intensities are influenced by excitation temperature variations caused by e.g, the molecular outflow or by molecular depletion. Assuming a distance of 630 pc the size of the CG 12 compact head, 1.1 pc by 1.8 pc, and the C18O mass larger than 100 Msun are comparable to those of other nearby low/intermediate mass star formation regions.

Ground State Rotational Lines of Doubly Deuterated Ammonia as Tracers of the Physical Conditions and Chemistry of Cold Interstellar Medium

We report the first detection of the N = 111 → 000 and 110 → 000 ground state rotational lines of o-ND2H at 335.5 and 388.7 GHz, obtained in the Lynds 1689N, Barnard 1, and Lynds 1544 molecular clouds using the Caltech Submillimeter Observatory (CSO). The submillimeter ND2H lines have moderate opacities and simple hyperfine patterns, which allow accurate determination of the excitation temperature, H2 volume density, and molecular column density. Both transitions have high critical densities. The 389 GHz line, in particular, traces molecular material with densities above a few × 106 cm-3. The strong 389 GHz ND2H emission in LDN 1689N implies a high fraction of dense gas in this source, ~30%, as compared to ~15% in B1 and LDN 1544. All these regions are sites of strong molecular depletion and heavy deuteration. Nonaccreting molecules, H and its isotopologues, are difficult to study, but in the sources studied here it appears that ammonia and its isotopologues are not completely frozen out, even in the high density gas. In the well-studied case of LDN 1544, the volume probed by the ND2H emission has densities of ~106-107 cm-3, within the range where the complete freezeout has been predicted to occur. The critical density of the 389 GHz ND2H line is close to that of the 309 GHz ND3 line. Observations of these two transitions thus provide an accurate measure of the [ND3]/[ND2H] fractionation ratio in the very dense gas. The [ND3]/[ND2H] ratio in LDN 1689N (~3%) appears lower than the values measured in B1 and LDN 1544 (~7%-10%), indicating that different chemical processes may be at work in these environments. The submillimeter lines of deuteroammonia are relatively strong and detectable from good sites, such as Mauna Kea or Chajnantor. Interferometric observations of these lines with the Submillimeter Array (SMA), and subsequently the Atacama Large Millimeter Array (ALMA), will provide new opportunities to study the physics and chemistry of cold, dense ISM, where most molecules are depleted onto dust grains.

Modeling the Physical Structure of the Low‐Density Pre‐Protostellar Core Lynds 1498

Lynds 1498 is a pre-protostellar core (PPC) and was one of the initial objects toward which molecular depletion and differentiation was detected. Despite the considerable scrutiny of L1498, there has not been a extensive study of the density and temperature structure as derived from radiative transfer modeling of dust continuum observations. We present deep SCUBA observations of L1498 at 850 and 450 micron, high resolution BEARS maps of the N2H+ 1-0 transition, CSO observations of the N2H+ 3-2 transition, and GBT observations of the C3S 4-3 transition. We also present a comparison of derived properties between L1498 and nearby PPCs that have been observed at far-infrared and submillimeter wavelengths. We present a more realistic treatment of PPC heating which varies the strength of the ISRF, Sisrf, and includes attenuation of the ISRF due to dust grains at the outer radius of the core, Av. The best-fitted model consists of a Bonner-Ebert sphere with a central density of 1 - 3 x 10^4 cm-3, R_o ~ 0.29 pc, 0.5 <= Sisrf <= 1, Av ~ 1 mag, and a nearly isothermal temperature profile of ~ 10.5 K for OH8 opacities. C3S emission shows a central depletion hole while N2H+ emission is centrally peaked. The observed depletions of C3S and H2CO, the modest N2H+ abundance, and a central density that is an order of magnitude lower than other modeled PPCs suggests that L1498 may be a forming PPC. Our derived temperature and density profile will improve modeling of molecular line observations that will explicate the core's kinematical and chemical state. (abridged)

Velocity field and star formation in the Horsehead nebula

Using large scale maps in C18O(2-1) and in the continuum at 1.2mm obtained at the IRAM-30m antenna with the Heterodyne Receiver Array (HERA) and MAMBO2, we investigated the morphology and the velocity field probed in the inner layers of the Horsehead nebula. The data reveal a non--self-gravitating (m/mvir = 0.3) filament of dust and gas (the "neck", diameter = 0.15-0.30 pc) connecting the Horsehead western ridge, a Photon-Dominated Region illuminated by sigmaOri, to its parental cloud L1630. Several dense cores are embedded in the ridge and the neck. One of these cores appears particularly peaked in the 1.2 mm continuum map and corresponds to a feature seen in absorption on ISO maps around 7 micr. Its \cdo emission drops at the continuum peak, suggestive of molecular depletion onto cold grains. The channel maps of the Horsehead exhibit an overall north-east velocity gradient whose orientation swivels east-west, showing a somewhat more complex structure than was recently reported by \cite{pound03} using BIMA CO(1-0) mapping. In both the neck and the western ridge, the material is rotating around an axis extending from the PDR to L1630 (angular velocity=1.5-4.0 km/s). Moreover, velocity gradients along the filament appear to change sign regularly (3 km/s/pc, period=0.30 pc) at the locations of embedded integrated intensity peaks. The nodes of this oscillation are at the same velocity. Similar transverse cuts across the filament show a sharp variation of the angular velocity in the area of the main dense core. The data also suggest that differential rotation is occurring in parts of the filament. We present a new scenario for the formation and evolution of the nebula and discuss dense core formation inside the filament.

N2H+] OBSERVATIONS OF MOLECULAR CLOUD CORES IN TAURUS

We report the millimeter-wave radio observations of molecular cloud cores in Taurus. The observed line is the <TEX>$N_2H^+$</TEX> emission at 93 GHz, which is known to be less affected by molecular depletion. We have compared starless (IRAS-less) cores with star-forming cores. We found that there is no large difference between starless and star-forming cores, in core radius, linewidth, core mass, and radial intensity profile. Our result is in contrast with the result obtained by using a popular molecular line, in which starless cores are larger and less condensed. We suggest that different results mainly come from whether the employed molecular line is affected by depletion or not. We made a virial analysis, and found that both starless and star-forming cores are not far from the critical equilibrium state, in Taurus. Together with the fact that Taurus cores are almost thermally supported, we conclude that starless Taurus cores evolve to star formation without dissipating turbulence. The critical equilibrium state in the virial analysis corresponds to the critical Bonnor-Ebert sphere in the Bonnor-Ebert analysis (Nakano 1998). It is suggested that the initial condition of the molecular cloud cores/globules for star formation is close to the critical equilibrium state/critical Bonnor-Ebert sphere, in the low-mass star forming region.

On the internal structure of starless cores

We have characterized the physical structure and chemical composition of two close-to-round starless cores in Taurus-Auriga, L1498 and L1517B. Our analysis is based on high angular resolution observations in at least two transitions of NH3 ,N 2H + ,C S, C 34 S, C 18 O, and C 17 O, together with maps of the 1.2 mm continuum. For both cores, we derive radial profiles of constant temperature and constant turbulence, together with density distributions close to those of non-singular isothermal spheres. Using these physical conditions and a Monte Carlo radiative transfer model, we derive abundance profiles for all species and model the strong chemical differentiation of the core interiors. According to our models, the NH3 abundance increases toward the core centers by a factor of several (≈5) while N2H + has a constant abundance over most of the cores. In contrast, both C 18 O and CS (and isotopomers) are strongly depleted in the core interiors, most likely due to their freeze out onto grains at densities of a few 10 4 cm −3 . Concerning the kinematics of the dense gas, we find (in addition to constant turbulence) a pattern of internal motions at the level of 0.1 km s −1 . These motions seem correlated with asymmetries in the pattern of molecular depletion, and we interpret them as residuals of core contraction. Their distribution and size suggest that core formation occurs in a rather irregular manner and with a time scale of a Myr. A comparison of our derived core properties with those predicted by supersonic turbulence models of core formation shows that our Taurus cores are much more quiescent than representative predictions from these models. In two appendices at the end of the paper we present a simple and accurate approximation to the density profile of an isothermal (Bonnor-Ebert) sphere, and a Monte Carlo-calibrated method to derive gas kinetic temperatures using NH3 data.

The NH3 / N2H+ abundance ratio in dense cores

We have observed the dense cores Barnard 217 (B217) and LDN 1262 (L1262) in the $(J,K)=(1,1)$ and $(2,2)$ inversion lines of ammonia and in the $J=1\mbox{--}0$ rotational line of diazenylium. The two cores are morphologically similar, in the sense that both contain a Class-I young stellar object (YSO) 1–2´ away from the core centre. The NH 3 and N 2 H + column densities show the same pattern in both cores: the average NH 3 /N 2 H + abundance ratios are 140–190 in the starless main bodies of the cores, while they drop to about 60–90 in the regions around the YSOs. Comparison with the dust continuum emission of B217 suggests that this pattern is due to an enhanced fractional ammonia abundance in the quiescent part of the core, where we find 3 . On the outskirts of the core the fractional ammonia abundance is about $\ensuremath{{3}\times10^{-8}}$, in accordance with our previous results. We discuss these findings in the light of recent chemical models including molecular depletion.

L1521E: The first starless core with no molecular depletion

L1521E seems unique among starless cores. It stands out in a distribution of a ratio (R) that we define to asses core evolution, and which compares the emission of the easily-depleted C18O molecule with that of the hard to deplete, late-time species N2H+. While all cores we have studied so far have R ratio lower than 1, L1521E has an R value of 3.4, which is 8 times the mean of the other cores. To understand this difference, we have modeled the C18O and N2H+ abundance profiles in L1521E using a density distribution derived from 1.2mm continuum data. Our model shows that the C18O emission in this core is consistent with constant abundance, and this makes L1521E the first core with no C18O depletion. Our model also derives an unusually low N2H+ abundance. These two chemical peculiarities suggest that L1521E has contracted to its present density very recently, and it is therefore an extremely young starless core. Comparing our derived abundances with a chemical model, we estimate a tentative age of 1.5 x 10^5 yr, which is too short for ambipolar diffusion models.

On The Internal Structure Of Starless Cores. Physical and Chemical Properties of L1498 and L1517B

We have characterized the internal structure of two close-to-round starless cores in Taurus, L1498 and L1517B, setting constraints on the initial conditions of star formation and on models of core condensation. Our analysis is based on high angular resolution observations in at least two transitions of NH3, N2H+, CS, C34S, C18O, and C17O, together with maps of the 1.2 mm continuum. For both cores, we derive radial profiles of constant temperature and constant turbulence, and density distributions close to those of non-singular isothermal spheres. Using a Monte Carlo radiative transfer model, we derive abundance profiles for all species and find a pattern of strong chemical differentiation. NH3 has a higher abundance toward the core centers while N2H+ has a constant abundance over most of the cores. Both C18O and CS (and isotopomers) are strongly depleted in the core interiors, most likely due to their freeze out onto cold dust grains. Concerning the kinematics of the dense gas, we find (in addition to constant turbulence) a pattern of internal motions at the level of 0.05 km s−1. These motions seem correlated with asymmetries in the pattern of molecular depletion, and we interpret them as residuals of core contraction. Their distribution and size suggest that core formation happens in rather irregular manner. A comparison with supersonic turbulence models of core formation shows that our observed cores are much more quiescent than allowed by these models.

Continuum and CO/HCO+Emission from the Disk Around the T Tauri Star LkCa 15

We present Owens Valley Radio Observatory Millimeter Array λ = 3.4-1.2 mm dust continuum and spectral line observations of the accretion disk encircling the T Tauri star LkCa 15. The 1.2 mm dust continuum emission is resolved and gives a minimum diameter of 190 AU and an inclination angle of 57° ± 5°. There is a noticeable but, at present, poorly constrained decrease in the continuum spectral slope with frequency that may result from the coupled processes of grain growth and dust settling. Imaging of the fairly intense emission from the lowest rotational transitions of CO, ^(13)CO, and HCO^+ reveals a rotating disk substantially larger than that observed in the dust continuum. Emission extends to ~750 AU and the characteristic radius of the disk is determined to be ~425 AU (HWHM), based on model fits to the CO velocity field. The measured line ratios demonstrate that the emission from these species is optically thick, while that from C^(18)O and H^(13)CO^+ is optically thin, or nearly so. The disk mass derived from the CO isotopologues with typical dense cloud abundances is still nearly 2 orders of magnitude less than that inferred from the dust emission, the most probable explanation being extensive molecular depletion in the cold, dense disk midplane. Thus, while CO, HCO^+, and their isotopologues are excellent tracers of the disk velocity field, they are not reliable tracers of the disk mass. N_2H^+ 1 → 0 emission has also been detected which, along with HCO^+, sets a lower limit to the fractional ionization of 10^(-8) in the near-surface regions of protoplanetary disks. This first detection of N_2H^+ in circumstellar disks has also made possible a determination of the N_2/CO ratio (~2) that is at least an order of magnitude larger than those in the envelopes of young stellar objects and dense clouds. The large N_2/CO ratio indicates that our observations probe disk layers in which CO is depleted but some N_2 remains in the gas phase. Such differential depletion can lead to large variations in the fractional ionization with height in the outer reaches of circumstellar disks and may help to explain the relative nitrogen deficiency observed in comets.

C$\mathsf{^{18}}$O abundance in the nearby globule Barnard 68

We have studied the radial variation of the CO abundance in the nearby isolated globule Barnard 68 (B68). For this purpose, B68 was mapped in the three rotational lines , and . Using the recent discovery of Alves et al. ([CITE]) that the density structure of B68 agrees with the prediction for a pressure bound distribution of isothermal gas in hydrostatic equilibrium (Bonnor-Ebert sphere), we show that the flat CO column density distribution can be explained by molecular depletion. By combining the physical model with the observed CO column density profile, it was found that the density dependence of the CO depletion factor can be well fitted with the law , which is consistent with an equilibrium between the accretion and the desorption processes. In the cloud centre, between 0.5% and 5% of all CO molecules are in the gas phase. Our observations suggest a kinetic temperature of ≈8 K. In combination with the assumption that B68 is a Bonnor-Ebert sphere, this leads to a distance of 80 pc. The cloud mass consistent with these values is 0.7 , considerably less than previously estimated. We find in B68 no clear deviance of the near-infrared reddening efficiency of dust grains per unit column density with respect to values derived in diffuse clouds.

Radio-millimetre investigation of galactic infrared dark clouds

This paper presents follow-up observations of the mid-Infrared dark clouds selected from the ISOGAL inner Galaxy sample. On-the-fly maps of 13CO, C18O and the 1.2 mm continuum emission have been obtained a the IRAM 30-m telescope. A preliminary analysis of the physico-chemical conditions at works in the clouds is given and molecular depletion onto cold grains is discussed.

Molecular Depletion and Thermal Balance in Dark Cloud Cores

We analyze the effects of molecular depletion on the thermal balance of well-shielded, quiescent dark cloud cores. Recent observations of the significant depletion of molecules from the gas phase onto grain surfaces in dark clouds suggest the possibility that the gas-phase cooling in these regions is greatly reduced and consequently that gas kinetic temperatures might be increased. We reexamine cooling and heating processes in light of possible molecular depletion, including the effect of coupling between the gas and the grains. At densities ≤103.5 cm-3, the gas temperature can be significantly increased by the depletion of coolant species without significantly affecting the dust temperature because of the relatively weak gas-dust coupling. At higher densities, this coupling becomes sufficiently rapid to overwhelm the effect of the reduced gas-phase cooling, and depletion has little effect on the gas temperature while raising the dust temperature 1 K. The result is that depletion at densities ≥104.5 cm-3 can proceed without being evident as an enhanced gas temperature or without self-limiting due to an increase in the dust temperature increasing the desorption rate. This is consistent with observations of depletion in cold, dense regions of quiescent molecular clouds. It also suggests that depletion in moderate density regions can increase the thermal gas pressure, effectively enhancing the confinement of denser portions of molecular clouds and possibly accelerating the collapse of cloud cores.