Lignin was deposited at the surface of glassy carbon electrode potentiostatically. Cyclic voltammetric investigations revealed the surface modification of glassy carbon electrode by an electroactive polymer film. Lignin modified electrode showed catalytic activity towards the oxidation of histamine in phosphate buffer solution. A limit of detection of 0.28×10−6molL−1 (LoD=3s/slope) was achieved in the linear concentration range of 5×10−6 to 2×10−4molL−1. Along with the excellent recoveries in the range 92.76-100.20% of spiked histamine from human urine and red wine samples, the interference and stability results showed the potential applicability of the developed method for the determination of histamine in real samples.