Moderate-intensity Training Research Articles

Intermittent low-intensity and moderate-intensity exercise effects on cognition in community-dwelling older adults: a pilot study exploring biological mechanisms.

To examine the cognitive benefits of 6 months of prescribed intermittent exercise (10-min bouts totaling 150 weekly minutes) in community-dwelling older adults, comparing effects of low-intensity movement (LIM) and moderate-intensity aerobic exercise (aerobic exercise; AE) training; and exploring biological mechanisms of exercise-related cognitive improvement. Twenty-five adults (>60 years old) participated in a 6-month controlled trial and were randomized into LIM or AE intermittent training. Cognition was assessed using a neuropsychological test battery including the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS), California Verbal Learning Test, 2nd Edition (CVLT-II), and Delis-Kaplan Executive Function System (D-KEFS). Neuroimaging measures were collected using a 7 T human MRI scanner. Serologic neurotrophic and inflammatory factors were analyzed using Luminex multiplex assays [brain derived neurotrophic factor (BDNF); vascular endothelial growth factor (VEGF)]; interleukin-6 (IL-6), C-reactive protein (CRP), plasminogen activator inhibitor (PAI-1). LIM and AE intermittent training had dissociable effects on cognition, with LIM resulting in improved learning and memory and AE resulting in improved executive functioning. Intervention groups differed on change in cognitive performance on CVLT-II learning and D-KEFS trail making test. Increase in right dorsolateral prefrontal cortex (DLPFC) surface area was linked to executive improvement (i.e., phonemic fluency) regardless of intervention group. A decline in circulating PAI-1 was linked to learning and memory improvement in response to LIM over 6 months. Moderate-intensity AE and LIM intermittent training likely have distinct cognitive benefits, though low-intensity activity is often included as a control group in exercise trials in aging.

Using training impulse and monotony methods to monitor aerobic training load in rats.

This study is the first to apply training impulse (TRIMP) and Training Monotony (TM) methodologies, within the realm of sport science, in animal model studies. Rats were divided into Sedentary (SED, n=10) and Training (TR, n=13). TR performed a four-week moderate-intensity interval training with load progression. Lactate kinetics, lactate training impulse (TRIMPLac), maximal speed training impulse (TRIMPSmax) and TM were utilized to develop and monitor training protocol. TR showed an 11.9% increase in time to exhaustion at the second maximum incremental test and a 17.5% increase at the third test. External work was increased by 17.8% at the second test and 30.3% at the third. There was a 10.6% increase in external work at the third test compared to the second for TR. No difference in TRIMPLac between the 1st week (94±9 A.U) and 3rdweek (83±10 A.U) were seen. TRIMPSmax was 2400 A.U. in the 1st week, 2760 A.U. in the 2nd and 3rd weeks, and 3120 A.U. in the 4th week. The TM remained at 1.24 A.U throughout the protocol and there was no dropouts. TRIMPLac and TRIMPSmax contributed to the development and monitoring loads, demonstrating their potential to improve the accuracy of training protocols in animal model research.

Dose-response effects of exercise on mental health in community-dwelling older adults: Exploration of genetic moderators

Background/Objective(1) Examine the role of exercise intensity on mental health symptoms in a community-based sample of older adults. (2) Explore the moderating role of genetic variation in brain-derived neurotrophic factor (BDNF) and apolipoprotein E (APOE) on the effects of exercise on mental health symptoms. MethodThis study is a secondary analysis of a three-arm randomized controlled trial, comparing the effects of 6 months of high-intensity aerobic training vs. moderate-intensity aerobic training vs. a no-contact control group on mental health symptoms assessed using the Depression, Anxiety, and Stress Scale (DASS). The BDNF Val66Met polymorphism and APOE ε4 carrier status were explored as genetic moderators of exercise effects on mental health symptoms. ResultsThe exercise intervention did not influence mental health symptoms. The BDNF Val66Met polymorphism did not moderate intervention effects on mental health symptoms. APOE ε4 carrier status moderated the effect of intervention group on perceived stress over 6 months, such that APOE ε4 carriers, but not non-carriers, in the high-intensity aerobic training group showed a decline in perceived stress over 6 months. ConclusionsAPOE ε4 carrier status may modify the benefits of high-intensity exercise on perceived stress such that APOE ε4 carriers show a greater decline in stress as a result of exercise relative to non-APOE ε4 carriers.

Эффективность программы ранней реабилитации с применением дистанционных технологий на амбулаторном этапе у пациентов после хирургической коррекции приобретенных клапанных пороков сердца

Aim: to investigate the clinical safety and efficacy of a combined rehabilitation consisting of early rehabilitation with physical training in the hospital and remote rehabilitation using a mobile application in the outpatient setting after surgery for acquired valvular heart disease (VHD) using bypass Patients and Methods: the study involved 68 patients who underwent surgery for VHD, with 34 patients in the study group and 34 patients in the control group, based on their rehabilitation approach The study group consisted of two subgroups based on the type of rehabilitation. The subgroup that underwent complex rehabilitation (n=16) participated in treadmill training for 2 weeks in the inpatient setting at least 8 days after surgery, followed by remote rehabilitation in the outpatient setting using a mobile application. The subgroup that underwent remote rehabilitation (n=18) did not undergo early inpatient treadmill training. The control group only received standard cardiac rehabilitation methods Results: there were no significant differences in the rate or type of complications during the early postoperative period A more significant improvement in functional status was reported in the subgroup that underwent complex rehabilitation 24 days after surgery, compared to patients who did not receive early physical training as part of their rehabilitation program. The subgroup undergoing complex rehabilitation showed a significantly greater improvement in exercise tolerance (p=0.03) and peak oxygen uptake (p=0.04) 24 days after surgery. These indices continued to increase (p=0.04; p=0.03) by month 4 of the follow-up compared to postoperative day 7. These changes were more significant in the complex rehabilitation group than in the remote rehabilitation group. Patients who underwent complex rehabilitation showed better adherence to using the mobile application for remote rehabilitation than patients who underwent remote rehabilitation (50% vs. 38.8%; p=0.04). Conclusion: the rehabilitation program for patients after surgery for VHD, which includes early moderate-intensity physical treadmill training followed by outpatient distance rehabilitation using a mobile application, is safe and effective This is demonstrated by a significant improvement in functional status Additionally, early initiation of physical training is associated with better adherence to further use of distance rehabilitation technologies KEYWORDS: valvular heart disease, heart surgery, postoperative cardiac rehabilitation, early rehabilitation, remote rehabilitation FOR CITATION: Lyapina I.N., Argunova Yu.A., Dren E.V.', Chereneva L.A., Barbarash O.L. Effectiveness of early rehabilitation program with remote technologies in the outpatient setting after surgery for acquired valvular heart disease. Russian Medical Inquiry. 2024;8(1):16–25 (in Russ.). DOI: 10.32364/2587-6821-2024-8-1-3.

RESPOSTAS FISIOLÓGICAS E PSICOAFETIVAS DE ADULTOS TREINADOS EM PROTOCOLOS AGUDOS DE HIIT

ABSTRACT High Intensity Interval Training (HIIT) promotes similar adaptations and in some cases, superior to continuous aerobic training of moderate intensity, even when performed at all-out intensities. In this condition, it tends to have the greatest physiological disturbance and accumulation of metabolites compared to submaximal intensities, being one of the main factors associated with the negative response of pleasure in training. Objective: To verify the relationship between physiological and psychophysiological responses in order to repeat the training of two HIIT protocols of mesm volume load and different periods of work in trained adults of both sexes. Methods: In a randomized experiment, the sample consisted of 9 individuals aged 28±5 years, body mass 69.6±14.2 kg, height 169±0.1cm, BMI 24.1±2.3 kg/m2 and body fat percentage 20.2±7.9% practitioners of structured aerobic physical exercises for at least six months. Two different HIIT protocols were performed on an exercise bike, being (I) protocol 1:0.5, with prescribed intensity (80-90% of MAX HR), (II) protocol 1:2 with prescribed intensity (all-out) with total duration of effort of 20 minutes and interval of at least 48 hours between each session. Heart rate (HR), systemic blood pressure (SBP), subjective perception of exertion (SPE), visual analog scale (VAS), affective response (AR), fun (PACES) and intention to repeat the session were analyzed. The statistical analysis used was ANOVA with Tukey's post-hoc (p<0.05). Results: The analyses of HR, SBP, VAS, PACES and SPE showed no significant difference between the training protocols. The affective response (AR and intention to repeat) showed a decline in the protocol 1:0.5, but without significance (p>0.05). Conclusion: The data allow us to conclude that the 1:2 protocol caused greater physiological disturbances during exertion and presented greater positive affective response and intention to repeat the exercise session, variables that may contribute to adherence to regular physical exercise. Level of Evidence I; Therapeutic studies - Investigation of treatment results.

Effects of moderate intensity intermittent training versus continuous training on indices of cardio-metabolic health in women with hyperlipidemia

Background: Moderate intensity intermittent and continuous training are in practice for muscle strengthening and physical activity. These trainings can be used for cardiometabolic health of hyperlipidemic patients. Objective: to determine the effects of moderate-intensity intermittent versus continuous training on indices of cardio-metabolic health in women with hyperlipidemia. Methods: A randomized clinical trial was conducted at Milestone Fitness Gym in Sialkot from August to December 2021. Women between 30 to 40 years old with hyperlipidemia were included by using a non-probability convenience sampling technique. A total of Twenty (n=20) participants were randomly divided into two groups by lottery method. Moderate-intensity intermittent training was given to Group A (n=10), and moderate-intensity continuous training was given to Group B (n=10) by the gym trainer 3 days per week for 5 weeks. The lipid profile for hyperlipidemia, step test, modified Borg Scale and Timed up-and-go test were used for cardiometabolic health and assessed at the baseline and after 5 weeks of training. Results: The mean age was 34±3.43 years. There was no significant difference (p≥0.05) in lipid profile, lipid profile, Borg scale, and time up and go test when between both groups analysis was done. While within-group analysis shows that there was a significant difference (p<0.05) in all outcome measures of both groups. Conclusion: It is concluded that both techniques, moderate-intensity intermittent versus continuous training were effective equally on indices of cardio-metabolic health in women with hyperlipidemia. Keywords: Continuous Training; Moderate Intensity Intermittent Training; Hyperlipidemia

Therapeutic effects of the combination of moderate-intensity endurance training and MitoQ supplementation in rats with isoproterenol-induced myocardial injury: The role of mitochondrial fusion, fission, and mitophagy

IntroductionMitochondrial dysfunction causes myocardial disease. This study investigated the effects of MitoQ alone and in combination with moderate-intensity endurance training (EX) on cardiac function and content and mRNA expression of several proteins involved in mitochondrial quality control in isoproterenol (ISO)-induced heart injuries MethodsSeven groups of CTL, ISO, ISO-EX, ISO-MitoQ-125, ISO-MitoQ-250, ISO-EX+MitoQ-125, and ISO-EX+MitoQ-250 were assigned. Rats were trained on a treadmill, and the MitoQ groups received MitoQ in drinking water for 8 weeks, starting one week after the induction of heart injury. Arterial pressure and cardiac function indices, mRNA expression, protein content, oxidant and antioxidant markers, fibrosis, and histopathological changes were assessed by physiograph, Real-Time PCR, immunofluorescence, calorimetry, Masson’s trichrome, and H&E staining, respectively. ResultsThe impacts of MitoQ-125, EX+MitoQ-125, and EX+MitoQ-250 on arterial pressure and left ventricular systolic pressure were higher than MitoQ-250 or EX alone. ± dp/dt max were higher in ISO-EX+MitoQ-125 and ISO-EX+MitoQ-250 than ISO-MitoQ-125 and ISO-MitoQ-250 groups, respectively. Histopathological scores and fibrosis decreased in ISO-EX, ISO-MitoQ-125, ISO-EX+MitoQ-125, and ISO-EX+MitoQ-250 groups. The restoration of MFN2, PINK-1, and FIS-1 changes was higher in ISO-EX+MitoQ-125 and ISO-EX+MitoQ-250 than ISO-EX, ISO-MitoQ-125 and ISO-MitoQ-250 groups. The expression of MFN2 and PINK-1 was lower in ISO-MitoQ-125 and ISO-EX+MitoQ-125 than ISO and CTL groups. The expression of FIS-1 in ISO-EX and ISO-EX+MitoQ-250 increased compared to CTL and ISO groups. MDA decreased in ISO-MitoQ-125 and ISO-EX+MitoQ-125 groups. ConclusionExercise and MitoQ combination have additive effects on cardiac function by modulating cardiac mitochondria quality. This study provided a possible therapy to treat heart injuries.

Aerobic training and vitamin E administration ameliorates cardiac apoptosis markers in rats exposed to methamphetamine.

Methamphetamine (MA) abuse is related to risks to the cardiovascular system. The present study aimed to compare the effects of moderate-intensity aerobic training (MIAT) and vitamin E (Vit.E) supplementation on markers of cardiac apoptosis following MA exposure. Fifty-four rats were randomly divided into six groups. CON group did not receive MA, while the others received MA alone or in combination with MIAT, Vit. E, MIAT+Vit E, or paraffin (PAR). These groups received MA incrementally for 23 consecutive days. Vit.E and MIAT+Vit.E groups received vitamin E three times a week for six weeks. MIAT and MIAT+Vit.E groups exercised for 25-40 min. Immunohistochemical and gene expression analyses were performed on the heart tissues. Bax and TGF-β expression was significantly higher, while Bcl-2 and VEGF expression was significantly lower in the MA and PAR groups than in the other groups (p < 0.05). Bcl-2 and VEGF expression was higher, and Bax and TGF-β expression was significantly lower in the MIAT and MIAT+Vit.E groups than in the other groups (p < 0.05). In Vit.E treated groups, Bax and TGF-β expression were lower, and VEGF was higher than that in the MA and PAR groups, but higher than those in the CON, MIAT and MIAT+Vit.E groups. MA increased the expression of Bax and TGF-β, and decreased the expression of Bcl-2 and VEGF, suggesting increased cardiac apoptosis. In contrast, MIAT and Vit.E decreased the expression of Bax and TGF-β, suggesting a reduction in cardiac apoptosis induced by MA.

Exercise-induced neuroplasticity in autonomic nuclei restores the cardiac vagal tone and baroreflex dysfunction in aged hypertensive rats.

Aging is accompanied by considerable deterioration of homeostatic systems, such as autonomic imbalance characterized by heightened sympathetic activity, lower parasympathetic tone, and depressed heart rate (HR) variability, which are aggravated by hypertension. Here, we hypothesized that these age-related deficits in aged hypertensive rats can be ameliorated by exercise training, with benefits to the cardiovascular system. Therefore, male 22-mo-old spontaneously hypertensive rats (SHRs) and age-matched Wistar Kyoto (WKY) submitted to moderate-intensity exercise training (T) or kept sedentary (S) for 8 wk were evaluated for hemodynamic/autonomic parameters, baroreflex sensitivity, cardiac sympathetic/parasympathetic tone and analysis of dopamine β-hydroxylase (DBH+) and oxytocin (OT+) pathways of autonomic brain nuclei. Aged SHR-S versus WKY-S exhibited elevated mean arterial pressure (MAP: +51%) and HR (+20%), augmented pressure/HR variability, no cardiac vagal tone, and depressed reflex control of the heart (HR range, -28%; gain, -49%). SHR-T exhibited a lower resting HR, a partial reduction in the MAP (-14%), in the pressure/HR variabilities, and restored parasympathetic modulation, with improvement of baroreceptor reflex control when compared with SHR-S. Exercise training increased the ascending DBH+ projections conveying peripheral information to the paraventricular nucleus of hypothalamus (PVN), augmented the expression of OT+ neurons, and reduced the density of DBH+ neurons in the rostral ventrolateral medulla (RVLM) of SHR-T. Data indicate that exercise training induces beneficial neuroplasticity in brain autonomic circuitry, and it is highly effective to restore the parasympathetic tone, and attenuation of age-related autonomic imbalance and baroreflex dysfunction, thus conferring long-term benefits for cardiovascular control in aged hypertensive individuals.NEW & NOTEWORTHY Exercise training reduces high blood pressure and cardiovascular autonomic modulation in aged hypertensive rats. The dysfunction in the baroreflex sensitivity and impaired parasympathetic tone to the heart of aged hypertensive rats are restored by exercise training. Exercise induces beneficial neuroplasticity in the brain nuclei involved with autonomic control of cardiovascular function of aged hypertensive rats.

Exercise training reduces systemic inflammation and improves general health status in female migraineurs: a randomised controlled trail.

The objectives of this study were to assess the effect of 8 weeks of moderate-intensity aerobic training on permeability inflammatory indicators of matrix metalloproteinases (MMPs) and specific tissue inhibitors of MMPs in female migraineurs. Female migraineurs (n = 28, age 32 ± 6) were randomised into two groups: migraine with exercise training (EXE + Mig, n = 13) and migraine without exercise training (NON-EXE + Mig, n = 15). Matched healthy women were also recruited as a healthy control group (CON, n = 15). The EXE-Mig group performed 8weeks of aerobic training. Pre and post intervention, serum matrix metalloproteinases (MMP-2 and 9) and specific tissue inhibitors of MMPs (TIMP-1 and 2) were measured. In addition, body composition indices and VO2max were determined. Exercise training reduced serum MMP-9 in female migraineurs with between-group changes and a time x group interaction (p < 0.05). In addition, exercise training reduced the serum MMP-9/TIMP-1 ratio in female migraineurs with between-group changes and time x group interaction(p < 0.05). However, no training-induced effect was observed in serum TIMP-1, TIMP-2, MMP-2 contents (p > 0.05) and MMP-2/TIMP-2 ratio (p > 0.05). Finally, exercise training reduced body fat content, WHR and BMI, and improved VO2max (p < 0.01). Our results demonstrated beneficial effects of aerobic exercise training on some circulatory inflammation factors (MMP9, MMP-9/TIMP-1) and some health indicators in female migraineurs, suggesting that such training can be employed as a non-pharmacological therapeutic method.

Effects of Different Protocols of Moderate-Intensity Intermittent Hypoxic Training on Mental Health and Quality of Life in Brazilian Adults Recovered from COVID-19: The AEROBICOVID Double-Blind Randomized Controlled Study

The aim of this study was to investigate the effects of different protocols of moderate-intensity intermittent hypoxic training in patients who had recovered from COVID-19 on quality of life (QoL) and mental health. The sample of this clinical trial-controlled double-blind study consisted of 67 participants aged 30–69 years, who were organized randomly according to Normoxia, Hypoxia, Hypoxia Recovery or Control Group. Eight weeks of cycle ergometer training were performed with a frequency of three training sessions per week in normoxic or hypoxic conditions (with or without hypoxic recovery). Health-related QoL and Mental Health Status were evaluated by 12-Item Short Form Survey and Depression Anxiety and Stress Scale instruments, respectively. All training groups improved the QoL’s physical dimensions (Baseline–Post: Normoxia Group 42.1 (11.0)–48.7 (7.0), Hypoxia Group 46.9 (11.8)–53.5 (6.6) and Hypoxia Recovery Group 45.8 (9.2)–51.1 (5.3)) and mental dimensions (Baseline–Post: Normoxia Group 48.8 (7.9)–54.6 (4.6), Hypoxia Group 45.2 (7.7)–53.2 (3.8) and Hypoxia Recovery Group 46.5 (9.7)–52.0 (9.9)). Regarding mental health outcomes, all training groups decreased depressive symptoms (66.7% Normoxia, 31.2% Hypoxia Recovery and 31% Hypoxia groups), anxiety symptoms (46.5% Normoxia, 45.9% Hypoxia Recovery and 39.5% in the Hypoxia groups) and stress symptoms (40.6% Normoxia, 36.3% Hypoxia Recovery and 22.1% Hypoxia groups). Significant statistical difference was not found between groups. Normoxic and hypoxic training showed a similar effect on QoL and the mental health of Brazilian adults who had recovered from COVID-19.

Effect of Exercise Training on Lipoprotein Subclass Particle Concentrations and Sizes in Older Women: Results from a Randomized Controlled Trial.

Evidence suggests that lipoprotein subclass particles are critical markers of cardiovascular disease (CVD) risk. Older women have increased CVD risk related to age. The purpose of this study was to determine whether low and moderate doses of exercise influence lipoprotein subclasses. Women (60-75 years) were randomized into groups for 16 weeks of moderate-intensity exercise training at a low or moderate dose (33.6 and 58.8 kJ/kg body weight weekly, respectively). Lipoprotein subclasses were determined by nuclear magnetic resonance spectroscopy before and after the training. The average weekly exercise duration was 109 and 164 min, for low- and moderate-dose groups, respectively. In the low-dose group, high-density lipoprotein particle (HDL-P) concentration decreased (Δ = -1.9 ± 3.1 µmol/L, mean ± SD, p = 0.002) and mean HDL-P size increased (Δ = 0.1 ± 0.3 nm, p = 0.028). In the moderate-dose group, mean HDL-P size (Δ = 0.1 ± 0.2 nm; p = 0.024) and low-density lipoprotein particle size increased (Δ = 0.4 ± 3.9 nm; p = 0.007). Baseline body mass index, peak oxygen consumption and age were associated with changes in a few lipoprotein subclasses. In this sample of inactive older women, moderate-intensity exercise training at a dose equivalent to or even lower than the minimally recommended level by public health agencies induced changes in lipoprotein subclasses in line with reduced CVD risk. However, higher doses are encouraged for greater health benefits.

From juniors to seniors: changes in training characteristics and aerobic power in 17 world-class cross-country skiers.

Purpose: To compare training characteristics and aerobic power (VO2max) between the most successful junior and senior seasons of world-class cross-country (XC) skiers and to identify differences between sexes and among sprint and distance skiers. Methods: Retrospective analysis was conducted on self-reported training and VO2max tests of ten male and seven female world-class XC-skiers, collectively holding 38 Olympic medals. Training was categorized by form (endurance, strength, speed, other) and mode (specific, unspecific) and was divided into low- (LIT), moderate- (MIT), and high-intensity training (HIT). Results: Total training increased by 203 ± 130h (35% ± 31%, p < .001, large effect) and 78 ± 69 sessions (21% ± 24%, p < .001, very large effect). Junior training volume (658 ± 107h) did not correlate with senior volume (861 ± 74h) but correlated negatively with changes in volume (r = -.822, p < .001). No sex differences were observed related to total volume, but distance skiers increased their total volume more than sprint skiers (p = .037, large effect). Endurance training increased by 197 ± 117h (p < .001; large effect) tied to increased low-intensity training (186 ± 115h, p < .001; large effect) and moderate-intensity training (13 ± 7h, p < .001; large effect). Training intensity distribution (% LIT/MIT/HIT) was 91/3/6 in junior and 92/4/4 in senior season. Women demonstrated greater increase of unspecific modes (100 ± 58 vs. 37 ± 44h, p = .022; large effect) and strength training (25 ± 23 vs. -3 ± 17h, p = .010, large effect). Men improved absolute (8% ± 5%; p = .009; large effect) and relative VO2max (6% ± 4%; p = .016; large effect) from junior to senior, while women only increased relative VO2max (7% ± 5%, p = .012; large effect). Conclusion: This study provides novel information regarding changes in training characteristics and aerobic power from junior to senior age in world-class XC-skiers. Overall, the enhanced training volume during this transition was primarily driven by increased LIT and MIT and the exceptionally high relative VO2max at junior age further increased in both sexes.

Effects of high intensity training on glycated hemoglobin type A1C reduction and insulin resistance changes compared to moderate intensity training in patients with type 2 diabetes : a systematic review and meta analysis

Background: In most of the type 2 Diabetes management recommends lifestyle including physical activity. Several studies have examined the effects of HIT and MIT on the glycated Hemoglobin type A1C (HbA1c) and Insulin resistance reduction in type 2 Diabetes. Objective: The objective of this meta-analysis was to investigate the impacts of high and moderate intensity training to reducing HbA1C and changes insulin resistance in type 2 Diabetes Melitus patient.State the objectives of the research. Material and Method: We searched the online databases of PubMed, ScienceDirect, and Cochrane Library for relevant studies evaluating the effects of High Intensity Training on T2DM patients. Using random effects model, comparing Mean Difference and standard deviation with 95% confidence internal (CI) were used to investigate the studies.Conclusion: In this study we found that HIT showed significantly better result to reduced HbA1C. HIT improves glucose control center’s ability to recruit more muscle fibers and depleting muscle glycogen levels compared to MIT, HIT does not significantly reduce insulin resistance compared to MIT.

Exercise therapy against platelet levels in obesity

The purpose of this study was to determine the effect of Moderate Intensity Training (MIT) and High Intensity Interval Training (HIIT) on the number of platelets used as a treatment option for obesity. Materials and methods. This research is a true experimental laboratory study with a post test only control group design. Using 21 obese male Wistar rats with a distribution of 7 Wistar HIIT groups, 7 Wistar MIT, and 7 control Wistar groups. The exercise intervention consisted of HIIT intensity (90-100% of baseline ability), MIT intensity (60-80% of baseline ability), and control without training for six weeks. A special mouse treadmill is used for exercise. Platelet measurement by enzyme-linked immunosorbent assay (ELISA). Hematology tests are used to determine platelets. Data analysis used the Anova test. Results. There was a significant difference in platelets between the control, MIT and HIIT groups (p = 0.015). The mean value of platelets in MIT and HIIT was higher than the control group. HIIT vs Control there was no significant difference (p = 0.816) and MIT vs Control there was a significant difference (p = 0.017). Likewise HIIT vs MIT there is no significant difference (P = 0.058). Conclusion. Exercise with HIIT and MIT for 6 weeks can be used as an alternative to overcome obesity because it can reduce weight in obesity and is accompanied by an increase in platelets.

Impact of an Eight-Week Moderate-Intensity Aerobic Training Program, Combined With Supplementation of the Aqueous Extract of Cynodon dactylon, on Hemorheological Indices in Young Non-athletic Men

Background: Cardiovascular diseases are the leading cause of death in developing countries and hemorheological indices are the main factors in developing these diseases. Objectives: The main objective of this study was to investigate the impact of eight weeks of moderate intensity aerobic training combined with supplementation of aqueous extract of Cynodon dactylon L. Pers on hemorheological parameters in non-athletic young men. Methods: A total of 40 young non-athletes (with a mean age of 35.63±6.43 years and a body mass index (BMI) of 23.55±2.13 kg/m2 ) were randomly assigned to one of four groups, including control (C), aerobic exercise (E), the C. dactylon extract (CD), and aerobic exercise+C. dactylon extract (E+CD). Changes in fibrinogen, blood and plasma viscosity, and hematocrit were measured at two stages (baseline and 24 hours after the intervention ended). Results: The blood fibrinogen level in three groups of E, CD, and E+CD showed a significant decline compared to the baseline status (P&lt;0.05). Additionally, there were significant decreases in blood viscosity levels in groups E (P=0.006), CD (P=0.048), and E+CD (P=0.001) after eight weeks of intervention. The plasma viscosity also showed a significant reduction only in group E after eight weeks of intervention (P=0.004). Moreover, hematocrit level significantly decreased in group E+CD (P=0.018). Conclusion: The findings of this study suggest that eight weeks of moderate intensity aerobic exercises alone and in combination with the consumption of the aqueous extract of C. dactylon L. Pers could improve hemorheological indices and reduce the risk of cardiovascular diseases.

The effect of low- and moderate-intensity interval training on cognitive behaviors of male and female rats with VPA-induced autism

IntroductionThis study was performed to evaluate the effects of low and moderate treadmill exercise for one month on social interaction, anxiety-like behaviors, and spatial learning and memory in male and female autistic rats. MethodsPregnant rats received valproic acid (VPA) (600 mg/kg/i.p) once on gestational day 12.5 to induce autism-like symptoms in the offspring. After delivery, the offspring were divided into six main groups, each with male and female subgroups: Control (CTL, prenatal normal saline), autism (prenatal VPA), low-intensity training (LIT,normal saline + low treadmill exercise), moderate -intensity training (MIT, normal saline + moderate treadmill exercise), VPA + LIT, and VPA + MIT. On the 60th day, the offspring were tested by the elevated plus maze (EPM), open field test (OFT), social interaction test (SIT), and Morris water maze (MWM). ResultsThe results showed that both LIT and MIT could partly alleviate anxiety-like behaviors induced by prenatal VPA exposure in two sexes. Social impairment was observed in the autistic rats and was improved by LIT in both sexes and MIT in females. No significant change was seen in the spatial learning and memory of autistic rats by exercise. ConclusionThe findings suggest that treadmill exercise can be helpful for improving some autism-like behaviors. Further studies are needed to investigate the involved mechanisms.

Resistance exercise training benefits pulmonary, cardiac, and muscular structure and function in rats with stable pulmonary artery hypertension

AimWe tested the effects of low- to moderate-intensity resistance exercise training (RT) on the structure and function of pulmonary, right ventricle (RV), and skeletal muscle tissues in rats with stable pulmonary artery hypertension (PAH). Main methodsAfter the first monocrotaline (MCT; 20 mg/kg) injection, male rats were submitted to a RT program (Ladder climbing; 55–65 % intensity), 5 times/week. Seven days later rats received the second MCT dose. Physical effort tolerance test and echocardiographic examination were performed. After euthanasia, lung, heart, and biceps brachii were processed for histological, single myocyte, and biochemical analysis. Key findingsRT improved survival and physical effort tolerance (i.e., maximum carrying load), mitigated the pulmonary artery resistance increase (i.e., TA/TE), and preserved cardiac function (i.e., fractional shortening, ejection fraction, stroke volume and TAPSE). RT counteracted oxidative stress (i.e., CAT, SOD, GST, MDA and NO) and adverse remodeling in lung (i.e., collapsed alveoli) and in biceps brachii (i.e., atrophy and total collagen) tissues. RT delayed RV adverse remodeling (i.e., hypertrophy, extracellular matrix, collagen types I and III, and fibrosis) and impairments in single RV myocyte contractility (i.e., amplitude and velocity to peak and relaxation). RT improved the expression of gene (i.e., miRNA 214) and intracellular Ca2+ cycling regulatory proteins (i.e., PLBser16); and of pathological (i.e., α/β-MHC and Foxo3) and physiological (i.e., Akt, p-Akt, mTOR, p-mTOR, and Bcl-xL) hypertrophy pathways markers in RV tissue. SignificanceLow- to moderate-intensity RT benefits the structure and function of pulmonary, RV, and skeletal muscle tissues in rats with stable pulmonary artery hypertension.

Moderate- and High-Intensity Endurance Training Alleviate Diabetes-Induced Cardiac Dysfunction in Rats.

Exercise training is an encouraging approach to treat cardiac dysfunction in type 2 diabetes (T2DM), but the impact of its intensity is not understood. We aim to investigate whether and, if so, how moderate-intensity training (MIT) and high-intensity interval training (HIIT) alleviate adverse cardiac remodeling and dysfunction in rats with T2DM. Male rats received standard chow (n = 10) or Western diet (WD) to induce T2DM. Hereafter, WD rats were subjected to a 12-week sedentary lifestyle (n = 8), running MIT (n = 7) or HIIT (n = 7). Insulin resistance and glucose tolerance were assessed during the oral glucose tolerance test. Plasma advanced glycation end-products (AGEs) were evaluated. Echocardiography and hemodynamic measurements evaluated cardiac function. Underlying cardiac mechanisms were investigated by histology, western blot and colorimetry. We found that MIT and HIIT lowered insulin resistance and blood glucose levels compared to sedentary WD rats. MIT decreased harmful plasma AGE levels. In the heart, MIT and HIIT lowered end-diastolic pressure, left ventricular wall thickness and interstitial collagen deposition. Cardiac citrate synthase activity, mitochondrial oxidative capacity marker, raised after both exercise training modalities. We conclude that MIT and HIIT are effective in alleviating diastolic dysfunction and pathological cardiac remodeling in T2DM, by lowering fibrosis and optimizing mitochondrial capacity.

Treadmill running intensity and post-exercise increase in plasma cardiac troponin I and T-A pilot study in healthy volunteers.

Plasma concentrations of cardiac troponins increase in healthy individuals after strenuous training, but the response to lower exercise intensities has not been characterized. To determine whether exercise at moderate intensity significantly increases plasma cardiac troponins measured with different assays in healthy recreational athletes. Twenty-four self-reported healthy volunteers were instructed to complete three 60-min bouts of treadmill running at variable intensities: High-intensity training (HIT) including a maximal exercise test and an anaerobic threshold test followed by training at 80%-95% of maximum heart rate (HRmax ), Moderate-intensity training (MIT) at 60%-75% of HRmax , and Low-intensity training (LIT) at 45%-55% of HRmax . Blood samples were collected before and at 2, 4, and 6 h after HIT and 4 h after MIT and LIT. Troponin I and T were measured in plasma samples with assays from Abbot, Siemens, and Roche. Plasma troponins measured with all assays were significantly increased compared to baseline after HIT but not after LIT. After HIT, the fraction of all participants with one or more values above the assay-specific 99th percentiles ranged from 13% to 61%. The biomarker criteria for acute myocardial injury were met after HIT for troponin T in 75% of female participants having no clinical evidence of coronary artery disease. High-intensity, but not moderate- or low-intensity, training for 60 min induced a potentially clinically significant increase in plasma cardiac troponins in healthy volunteers. Results exceeding the population 99th percentiles were most frequent with the troponin T assay.