With the large-scale integration of renewable energy and changes in load characteristics, the power system is facing challenges of volatility and instability. Therefore, enhancing the safe and stable operation capability of the power system is an urgent problem that needs to be solved. Mobile energy storage can improve system flexibility, stability, and regional connectivity, and has the potential to serve as a supplement or even substitute for fixed energy storage in the future. However, there are few studies that comprehensively evaluate the operational performance and economy of fixed and mobile energy storage systems. To comprehensively evaluate the economic benefits of large-scale mobile energy storage systems, this paper constructs an overall horizontal cost model for energy storage systems that considers the power system and train transportation system. This model not only introduces the traditional concept of lifecycle cost of energy storage systems, but also considers the costs of traditional transmission lines and battery transportation. Secondly, to achieve simulation of large-scale mobile energy storage system planning and operation, this paper establishes a multi-region power planning and operation simulation (MPO) model and a battery transportation and logistics (BTL) model to accurately reflect the operation mode of fixed energy storage and mobile energy storage in the power system. Finally, taking the actual power grids and railway networks in Northeast and North China as case studies, this article provides an in-depth analysis of the technical, economic, and environmental performance of large fixed and mobile energy storage systems, and conducts a detailed comparison. The research results indicate that under high grid connection ratios (using 75% and 66% as examples), the overall cost of mobile energy storage systems continues to decrease to 1.42 CNY/kWh and 0.98 CNY/kWh. Compared to fixed energy storage at 5.45 CNY/kWh and 4.79 CNY/kWh, it has an absolute economic advantage and shows significant carbon reduction capabilities, reaching 241,800 to 1,394,600 tons. This discovery fully confirms the enormous potential and application value of mobile energy storage in high proportion renewable energy scenarios, providing strong technical support and economic analysis basis for the sustainable development of the power system.