This paper explores integrating artificial intelligence (AI) segmentation models, particularly the Segment Anything Model (SAM), into fluid mechanics experiments. SAM’s architecture, comprising an image encoder, prompt encoder, and mask decoder, is investigated for its application in detecting and segmenting objects and flow structures. Additionally, we explore the integration of natural language prompts, such as BERT, to enhance SAM’s performance in segmenting specific objects. Through case studies, we found that SAM is robust in object detection in fluid experiments. However, segmentations related to flow properties, such as scalar turbulence and bubbly flows, require fine-tuning. To facilitate the application, we have established a repository (https://github.com/AliRKhojasteh/Flow_segmentation) where models and usage examples can be accessed.