A mixture of date palm pollen (dried or ethanol- extracted) and vitamin D3 IN 4000 IU and(dried or ethanol- extracted ) Eruca sativa were used to treat 20 albino mice to determine the activity of the testosterone hormones. The mice divided into five groups each group contains 5 of mice male groups( treated with ethanol extract of the mixture at 400, treated with ethanol extract of the mix at 500, treated with dried extract of the mixture at 400, and treated with dried extract of the mixture at 500) mg \kg respectively, significant differences in the results in the highest effect of 500mg/kg dried powder of the mixture of date palm pollen and vitamin d3 and Eruca sativa on group 4 at day week 6 compared to the control in group 5. The lower effect, according to the significant differences in group 1 at week three yet it’s still promising due to a higher level than control which is the control. Other were with treatment with gradual change for the extreme results in week six the testosterone level was measured gradually