As one of the major water supply systems for inland rivers, especially in arid and semi-arid regions, snow cover strongly affects hydrological cycles. In this study, remote sensing datasets combined with in-situ observation data from a route survey of snow cover were used to investigate the changes in snow cover parameters on the Chinese Altai Mountains from 2000 to 2022, and the responses of snow cover to climate and hydrology were also discussed. The annual snow cover frequency (SCF), snow cover area, snow depth (SD), and snow density were 45.03%, 2.27 × 104 km2, 23.4 cm, and ~0.21 g·cm−3, respectively. The snow water equivalent ranged from 0.58 km3 to 1.49 km3, with an average of 1.12 km3. Higher and lower SCF were mainly distributed at high elevations and on both sides of the Irtysh river. The maximum and minimum snow cover parameters occurred in the Burqin River Basin and the Lhaster River Basin. In years with high SCF, abnormal westerly airflow was favorable for water vapor transport to the Chinese Altai Mountains, resulting in strong snowfall, and vice versa in years with low SCF. There were significant seasonal differences in the impact of temperature and precipitation on regional SCF changes. The snowmelt runoff ratios were 11.2%, 25.30%, 8.04%, 30.22%, and 11.56% in the Irtysh, Kayit, Haba, Kelan, and Burqin River Basins. Snow meltwater has made a significant contribution to the hydrology of the Chinese Altai Mountains.