In this study, the lindane degrading bacterial isolate Mz-13i was isolated using the enrichment culture technique. The isolate was found positive for HCH utilization when screened through colorimetric assay. It was able to withstand higher concentrations of lindane up to 100 ppm. Further, the bacterium was cultured on mineral salt medium (MSM) agar plates, and its physiological, morphological, and biochemical studies and molecular analysis through 16S rRNA sequencing were carried out. The 16S rRNA gene sequence of the isolate was deposited to NCBI GenBank with accession number MF599367 and classified as Bacillus subtilis strain Mz-13i through phylogenetic analysis. Gas-Chromatography Mass-Spectroscopy (GC-MS) analysis indicated the peaks for pathway-specific metabolites, viz., for γ-PCCH (7.852), benzoquinone (9.604), benzene carboxylic acid (12.608), oxalic acid (30.308) and benzene derivatives were found. During degradation, the chloride ions releases such as 0.22 mg/ml (α-HCH), 0.27 mg/ml (β-HCH), 0.48 mg/ml (γ-HCH), 0.30 mg/ml (δ-HCH). The half-life period calculated was 1.35 days for γ-HCH and 15.0 days for β-HCH. From the degradation kinetics data, the γ-isomer of HCH mediated for degradation, whereas the β-isomer was hardly degradable. The degradation for four isomers was 58.55% (α-HCH), 15% (β-HCH), 78.25 (γ-HCH), and 56% (δ-HCH).