ABSTRACT Objective Our study objectives were to characterize steady-state plasma chlortetracycline (CTC) concentrations achieved by adult beef cows treated with a commercial, CTC-containing mineral supplement under 2 conditions, where intake was controlled and under conditions when intake was uncontrolled, as in a pasture setting typical of production practices for anaplasmosis control. Materials and Methods Thirty cows were allocated to 3 individually administered treatment groups: nonmedicated control (NM-F), medicated mineral administered via gelatin capsule (M-C), or medicated mineral fed in a small amount of grain (M-F). After a washout period, 15 of the original cows received an intended CTC dose of 1.1 mg·kg of BW−1·d−1 administered via a mineral feeder in a pasture setting (M-P). Blood samples were collected at multiple time points, and plasma chlortetracycline concentrations were quantified. Results and Discussion Mean plasma chlortetracycline concentrations for M-C and M-F treatments were 20.2 and 19.3 ng/mL, respectively, with no differences in mean, minimum, or maximum plasma concentrations. Conversely, differences in both median and minimum plasma concentrations were detected when the dose was controlled (M-C or M-F) compared with when the dose was administered via mineral feeder in a pasture setting (M-P). Implications and Applications Future CTC studies can be designed using individual intake in grain because administration by gelatin capsule offers no advantage in dosage consistency. The assumption that mean consumption from a mineral feeder will achieve the same plasma CTC concentrations as individual or bunk-fed experiments should be further analyzed. The pharmacodynamic parameters associated with efficacy were not evaluated in this study and remain unknown.