ABSTRACT Human activities and climatic changes have altered the hydrological ecosystem of the Min River Basin and affected in-river biodiversity. In this paper, the year of abrupt hydrological change was identified using multiple tests, and the drivers of ecohydrological change were quantified in conjunction with the Budyko coupled hydrothermal equilibrium theory. Combined with ecological flow indicators (ecological surplus (ES) and ecological deficit (ED)) calculated based on discharge hydrographs and multiple hydrological indicators (ES/ED), the degree of river hydrological alteration in the Min River Basin was comprehensively evaluated, and its impacts on in-river biodiversity analyzed. The results of the study showed that: (1) according to the Budyko theory, the influence of human activities on the runoff changes in the Min River Basin reached 56.80%, which was the main influence factor, followed by climatic factors (41.56% for precipitation and 1.64% for evapotranspiration); (2) dam construction has generally resulted in an increase in seasonal ES and a decrease in seasonal ED; (3) the combination of the ecological flow indexes with the ecologically relevant hydrological indicators not only reduces the redundancy between the parameters, but also reflects the essential hydrological information and ecological connotations, and it is an effective method for evaluating ecohydrological mechanisms.
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