Mimosa Tenuiflora Research Articles

A Validated RP-HPLC–DAD Method for Analyzing Flavonoids in Caatinga Brazilian Green Propolis from Mimosa tenuiflora Produced by Apis mellifera

A Validated RP-HPLC–DAD Method for Analyzing Flavonoids in Caatinga Brazilian Green Propolis from Mimosa tenuiflora Produced by Apis mellifera

New Technology of Rumen-Protected Bypass Lysine Encapsulated in Lipid Matrix of Beeswax and Carnauba Wax and Natural Tannin Blended for Ruminant Diets

Tannins are compounds present in forage plants that, in small quantities in the diet of ruminants, produce protein complexes that promote passage through the rumen and use in the intestine. This study tested the hypothesis that beeswax (BW) and carnauba wax (CW) lipid matrices are effective encapsulants for creating bypass lysine (Lys) for ruminants, with tannin extracted from the Mimosa tenuiflora hay source enhancing material protection. Microencapsulated systems were made using the fusion–emulsification technique with a 2:1 shell-to-core ratio and four tannin levels (0%, 1%, 2%; 3%). The following eight treatments were tested: BWLys0%, BWLys1%, BWLys2%, BWLys3%, CWLys0%, CWLys1%, CWLys2%, and CWLys3%. Tannin inclusion improved microencapsulation yield and efficiency. CWLys3% had the highest microencapsulation efficiency and retained Lys. Lysine in BW and CW matrices showed higher thermal stability than in its free form. Material retention was greater in BW than CW. Rumen pH and temperature remained unaffected, indicating that BW and CW as the shell and tannin as the adjuvant are efficient encapsulants for Lys bypass production. The formulation CWLys3% is recommended as it is more efficient in protecting the lysin amino acid from rumen degradation.

Green synthesis and thermoluminescent response of ZnO particles using Mimosa tenuiflora bark extract

Green synthesis and thermoluminescent response of ZnO particles using Mimosa tenuiflora bark extract

The anxiolytic-like effect of the alkaloid fraction of the psychedelic plant Mimosa tenuiflora (Willd.) Poir

The present work investigated the anxiolytic effect of the alkaloid fraction (AF II) from the root bark of Mimosa tenuiflora. Female Swiss mice of 6 weeks old were used in this study. The animals were divided into three groups: 0.9% NaCl (vehicle group, s.c.), AF II (6 mg/kg, s.c.), and diazepam – DZP (5 mg/kg, s.c.). The light/dark box and elevated plus maze tests evaluated the animal anxiety. DMT was identified by the HPLC method. AF II showed significant anxiolytic effects in the two behavioural tests used.

Unraveling the metabolomic profile and acute toxicity of ethanolic extract from Mimosa tenuiflora (Willd.) Poir. root bark

Mimosa tenuiflora (Fabaceae) is popularly known in Brazil as “Jurema preta”. From the bark of its root, “jurema wine” is obtained, a psychedelic drink used in Indigenous religious rituals in Northeastern Brazil. This work aimed to investigate the chemical composition and acute oral toxicity of the ethanolic extract of the root bark from M. tenuiflora (EEMt). EEMt was analyzed by UPLC-QToF-MS/MS and DI-ESI-IT-MSn. Oral administration of EEMt was performed once at doses of 300 and 2000 mg/kg in female Swiss mice. Signs and symptoms of intoxication, as well as mortality were monitored for 14 days. Thirteen compounds were annotated in EEMt: eight type B proanthocyanidins, three alkaloids, a glycosylated flavonol, and a dihydrochalcone derivative. The acute administration of 300 and 2000 mg/kg of EEMt did not show mortality. It also did not change the food intake or body weight of the animals. However, the relative weights of the kidneys were significantly changed for both doses. Changes in hematological and biochemical parameters were found. In addition, histopathological changes were also observed in the heart, liver, and kidneys. Thus, based on our findings, EEMt presented an LD50 greater than 2000 mg/kg and was therefore classified in category 5 of the Globally Harmonized Classification System (GHS). EEMt showed acute oral toxicity by altering hematological, biochemical and histological parameters.

Volumetric and energy production assessment of wood in managed forest in the Brazilian arid biome

There is a growing demand for wood products from forests located in dry regions, which includes the Caatinga, a biome in Northeast Brazil. This study evaluates the relationship between volumetric production, energy potential, and the rotation cycle. Information was collected from forest stands in different stages of regeneration located in an arid region of Brazil. Based on the forest management plan, four fields were selected with post-logging ages of 9, 11, 13, and 16 years. This inventory recorded circumference at chest height, circumference at base height, total height, volume, stored energy, technical cutting age, and rainfall index. The results showed that the species that presented the most significant quantity of stems did not always correspond to those that obtained the most significant amount of biomass. The technical cutting age was determined at 16 years, aiming to maximize wood utilization. Regarding energy density, the 9-year-old field reached 7,281 kcal ha-1, the 11-year-old field obtained 14,448 kcal ha-1, the 13-year-old field recorded 41,526 kcal ha-1, and the 16-year-old field reached 98,190 kcal ha-1. The species that contributed most to energy accumulation included Mimosa tenuiflora with 3,740 kcal m–³, Piptadenia stipulacea with 3,271 kcal m-3, and Cenostigma pyramidale with 3,101 kcal m-3.

The role of ayahuasca in cell viability and oxidative stress in gastric adenocarcinoma cell line

Ayahuasca, a psychoactive beverage native to the Amazon, originally derived from Banisteriopsis caapi stem scrapings and Psychotria viridis leaves, exhibits hallucinogenic properties due to N,N-dimethyltryptamine. When combined with β-carbolines, it enters the bloodstream and central nervous system, inhibiting monoamine oxidase-A. Over time, therapeutic effects have been associated to ayahuasca consumption. This study assessed the impact of extracts from three plant decoctions used in ayahuasca preparation on the gastric adenocarcinoma cell line (AGS). MTT reduction assays selected B. caapi, Mimosa hostilis, and Peganum harmala samples as most effective. Lactate dehydrogenase activity evaluated membrane integrity loss, while oxidative stress induction was measured using dihydroethidium and 2′,7′-dichlorodihydrofluorescein diacetate probes. Results revealed apoptosis induction in AGS cells, with all three samples significantly reducing oxidative stress.

Antimicrobial Potential of Chitosan Films Incorporated with Alcoholic Extract from Mimosa tenuiflora Leaves.

Antimicrobial films were prepared with chitosan containing the methanolic extract of M. tenuiflora leaves (FECT20%, FECT30%, and FECT40%), and their antimicrobial activities were evaluated by agar diffusion. The films were characterized by IR spectroscopy, scanning electron microscopy (SEM) and TG/DTG curves. TG/DTG curves showed thermal stability of chitosan-extract films up to 166 ºC. Micrographs of chitosan-extract films revealed an increase in porosity with the addition of extract. The FECT40% film showed inhibition zone diameters (IZ) againstMicrococcus luteus,Staphylococcus aureus,Bacillus subtilis, andB. cereus, ranging from1.0 ± 0.02 to 0.72 ± 0.09 cm. Only FECT30% and FECT40% inhibitedtheP. aeruginosawith IZs of 0.68 ± 0.02 and 0.77 ± 0.06 cm, respectively. In turn, the extract showed inhibition againstB. subtilisandB. cereus, with IZs values of 0.92 ± 0.2 cm and 0.72 ± 0.05 cm, respectively. Additionally, the crude extract presented antioxidant potential with inhibition percentages of 32.74% ± 0.90 for ABTS and 27.04% ± 1.36 for DPPH.The antimicrobial and antioxidant activities of the crude extract, as well as the antimicrobial property of chitosan-extract films, suggests the potential of these biopolymers for the development of wound healing bandages and new food packaging alternatives.

Flavonoids from Mimosa tenuiflora Green Propolis: Chemotaxonomy, Antioxidant, Anti‐Inflammation, Anti‐Bacteria, Mosquito Larvicidal Activity, and In Silico Approach

AbstractPhytochemical study of green propolis alcohol extract of Brazilian Caatinga Mimosa tenuiflora (Willd.) Poir resulted in the separation of six flavonoids, including quercetin 3‐methyl ether (1) and 3,3′‐O‐dimethylquercetin (2), and four flavanones eriodictyol 5‐O‐methyl ether (3), 5,4′‐dihydroxy‐6,7‐dimethoxyflavanone (4), licoflavanone (5), and macarangaflavanone B (6). All these metabolites were first isolated from green propolis and the genus Mimosa. Metabolites 3–4 also established chemotaxonomic significance since they had never been observed in the bean family Fabaceae. Flavones 1–2 showed strong antioxidative activity against DPPH (1,1‐diphenyl‐2‐picryl hydrazyl) radicals with IC50 values of 12.40 and 16.23 μg/mL, respectively. All six isolates showed anti‐inflammatory effects against NO (nitric oxide) production in LPS (lipopolysaccharide)‐stimulated Raw 264.7 cells, in which the activity of flavone 1 was better than the standard dexamethasone (IC50 13.35 μg/mL). Flavonoids 1–3 also strongly controlled the growth of the Gram‐positive bacteria Bacillus subtilis ATCC 6051, and Staphylococcus aureus ATCC 29213 with MIC values of 32–64 μg/mL. Six isolated flavonoids exhibited strong or moderate mosquito larvicidal activity against Aedes aegypti and Ae. albopictus fourth instar larvae with the 24 h LC50 values of 29.62–55.89 μg/mL. By in silico approach, compound 1 was a non‐toxic agent with an LD50 value of 5000 mg/kg.

The Role of Ayahuasca in Colorectal Adenocarcinoma Cell Survival, Proliferation and Oxidative Stress.

The psychedelic beverage ayahuasca is originally obtained by Banisteriopsis caapi (B. caapi) (BC) and Psychotria viridis (P. viridis) (PV). However, sometimes these plant species are replaced by others that mimic the original effects, such as Mimosa hostilis (M. hostilis) (MH) and Peganum harmala (P. harmala) (PH). Its worldwide consumption and the number of studies on its potential therapeutic effects has increased. This study aimed to evaluate the anticancer properties of ayahuasca in human colorectal adenocarcinoma cells. Thus, the maximum inhibitory concentration (IC50) of decoctions of MH, PH, and a mixture of these (MHPH) was determined. The activities of caspases 3 and 9 were evaluated, and the cell proliferation index was determined through immunocytochemical analysis (Ki-67). Two fluorescent probes were used to evaluate the production of oxidative stress and the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) was also evaluated. It was demonstrated that exposure to the extracts significantly induced apoptosis in Caco-2 cells, while decreasing cell proliferation. MH and MHPH samples significantly reduced oxidative stress and significantly increased glutathione peroxidase activity. No significant differences were found in SOD activity. Overall, it was demonstrated that the decoctions have a potential anticancer activity in Caco-2 cells.

Kaempferide triggers apoptosis and paraptosis in pancreatic tumor cells by modulating the ROS production, SHP-1 expression, and the STAT3 pathway.

Pancreatic cancer is one of the deadliest diseases with a poor prognosis and a five-survival rate. The STAT3 pathway is hyperactivated which contributes to the sustained proliferative signals in pancreatic cancer cells. We have isolated kaempferide (KF), an O-methylated flavonol, from the green propolis of Mimosa tenuiflora and examined its effect on two forms of cell death namely, apoptosis and paraptosis. KF significantly increased the cleavage of caspase-3 and PARP. It also downmodulated the expression of Alix (an intracellular inhibitor of paraptosis) and increased the expression of CHOP and ATF4 (transcription factors that promote paraptosis) indicating that KF promotes apoptosis as well as paraptosis. KF also increased intracellular reactive oxygen species (ROS) suggesting the perturbance of the redox state. N-acetylcysteine reverted the apoptosis- and paraptosis-inducing effects of KF. Some ROS inducers are known to suppress the STAT3 pathway and investigation revealed that KF downmodulates STAT3 and its upstream kinases (JAK1, JAK2, and Src). Additionally, KF also elevated the expression of SHP-1, a tyrosine phosphatase which is involved in the negative modulation of the STAT3 pathway. Knockdown of SHP-1 prevented KF-driven STAT3 inhibition. Altogether, KF has been identified as a promoter of apoptosis and paraptosis in pancreatic cancer cells through the elevation of ROS generation and SHP-1 expression.

Sensitivity and antioxidant response of forest species seedlings to the atrazine under simulated conditions of subsurface water contamination

Atrazine is an herbicide with a high soil leaching capacity, contaminating subsurface water sources. Once the water table is contaminated, riparian species can be exposed to atrazine. In this way, understanding the impacts of this exposure must be evaluated for planning strategies that minimize the effects of this herbicide on native forest species. We aimed to evaluate forest species' sensitivity and antioxidant response to exposure to subsurface waters contaminated with atrazine, as well the dissipation this herbicide. The experiment was conducted in a greenhouse in a completely randomized design, with three replications and one plant per experimental unit. The treatments were arranged in a 2 × 10 factorial. The first factor corresponded to the presence or absence (control) of the atrazine in the subsurface water. The second factor comprised 10 forest species: Amburana cearensis, Anadenanthera macrocarpa, Bauhinia cheilantha, Enterolobium contortisiliquum, Hymenaea courbaril, Libidibia ferrea, Mimosa caesalpiniifolia, Mimosa tenuiflora, Myracrodruon urundeuva, and Tabebuia aurea. The forest species studied showed different sensitivity levels to atrazine in subsurface water. A. cearensis and B. cheilantha species do not have efficient antioxidant systems to prevent severe oxidative damage. The species A. macrocarpa, E. contortisiliquum, L. ferrea, and M. caesalpiniifolia are moderately affected by atrazine. H. courbaril, M. urundeuva, and T. aurea showed greater tolerance to atrazine due to the action of the antioxidant system of these species, avoiding membrane degradation events linked to the production of reactive oxygen species (ROS). Among the forest species, H. courbaril has the most significant remedial potential due to its greater tolerance and reduced atrazine concentrations in the soil.

Vasorelaxant Activity of (2S)-Sakuranetin and Other Flavonoids Isolated from the Green Propolis of the Caatinga Mimosa tenuiflora.

Some in vitro and in vivo evidence is consistent with the cardiovascular beneficial activity of propolis. As the single actors responsible for this effect have never been identified, an in-depth investigation of flavonoids isolated from the green propolis of the Caatinga Mimosa tenuiflora was performed and their mechanism of action was described. A comprehensive electrophysiology, functional, and molecular docking approach was applied. Most flavanones and flavones were effective CaV1.2 channel blockers with a potency order of (2S)-sakuranetin > eriodictyol-7,3'-methyl ether > quercetin 3-methyl ether > 5,4'-dihydroxy-6,7-dimethoxyflavanone > santin > axillarin > penduletin > kumatakenin, ermanin and viscosine being weak or modest stimulators. Except for eriodictyol 5-O-methyl ether, all the flavonoids were also effective spasmolytic agents of vascular rings, kumatakenin and viscosine also showing an endothelium-dependent activity. (2S)-Sakuranetin also stimulated KCa1.1 channels both in single myocytes and vascular rings. In silico analysis provided interesting insights into the mode of action of (2S)-sakuranetin within both CaV1.2 and KCa1.1 channels. The green propolis of the Caatinga Mimosa tenuiflora is a valuable source of multi-target vasoactive flavonoids: this evidence reinforces its nutraceutical value in the cardiovascular disease prevention arena.

Performance and Surface Modification of Cast Iron Corrosion Products by a Green Rust Converter (Mimosa tenuiflora Extract)

One of the alternative materials used for conducting conservation treatment of iron artifacts is the rust converter, since it generates barrier properties and more stable oxides. The protective properties and surface modifications from using Mimosa tenuiflora extract as a green rust converter on a gray iron oxide layer were studied. The surface characterization was carried out using a Scanning Electron Microscope coupled to an energy-dispersive X-ray spectrometer (SEM-EDS), along with infrared spectroscopy (IR), Raman spectroscopy, X-ray Diffraction (XRD), and Water Contact Angle (WCA). Electrochemical characterization was performed with an Electrochemical Impedance Spectroscope (EIS) using 3.5 wt.% NaCl as the electrolyte. According to the results of the Raman spectroscopy and XRD, the layer of corrosion products formed after 90 days in the atmosphere was composed of goethite, lepidocrocite, maghemite, hematite, and magnetite. The surface of the corrosion products was transformed with the rust converter into an amorphous and microcracked layer. By IR, the Fe-O and C-O-Fe bonds associated with the iron chelate were found with absorption bands at 1540 and 1567 cm−1, respectively. By XRD, a modification of the magnetite crystallinity was observed. Finally, the Water Contact Angle and the protective capacity of the corrosion products were improved by the presence of the rust converter.

EIS study of Mimosa tenuiflora nanocontainers in a defective epoxy resin for the protection of low-carbon steel in a saline media

EIS study of Mimosa tenuiflora nanocontainers in a defective epoxy resin for the protection of low-carbon steel in a saline media

Natural Durability of Mimosa tenuiflora and Mimosa caesalpiniaefolia Woods: Two Species from the Brazilian Dry Forest

Natural Durability of Mimosa tenuiflora and Mimosa caesalpiniaefolia Woods: Two Species from the Brazilian Dry Forest

Gas exchange and phenotypic plasticity in three tree species in preserved and anthropized areas in a Tropical Dry Forest

Caatinga, the Brazilian tropical dry forest and source of endemic species, suffers anthropic disturbances that are responsible for affecting plants and their functional traits. Therefore, we aimed to evaluate the plasticity woody species’ leaf traits due to abiotic conditions of preserved and anthropized areas in Caatinga. The study took place in Vale do Riacho São José, in the municipality of Caetés, Pernambuco, Brazil. The selected species were Mimosa tenuiflora, Mimosa arenosa and Senna sp., all belonging to the Fabaceae family. We measured gas exchange, water content, specific leaf area and phenotypic plasticity index of all selected species in both areas. For all species, stomatal conductance was higher in preserved areas, but photosynthesis followed this pattern only for Senna sp. which also showed lower water use efficiency among all species. Furthermore, Senna sp. presented the highest relative water content in preserved areas, and showed no differences in specific leaf area between preserved and anthropized areas. The plasticity index showed that Senna sp. were the most plastic species, mainly adjusting water content and gas exchange parameters according to the area. The less plastic species was Mimosa arenosa. A principal component analysis showed a group formation due to anthropization, for all species. However, Senna sp. showed the largest distance among species. We conclude that all analyzed species respond to environmental conditions of preserved and anthropized areas, but this pattern cannot be related to the characteristics of the botanical family due to species-specific leaf traits’ variations.

Potentiality of goats and sheep on the survival and germination of Mimosa tenuiflora (Willd.) Poir. seeds from the Caatinga

Mimosa tenuiflora is a species widely distributed in the Caatinga biome as forage for herds. Goats and sheep are efficient seed dispersers and can improve seed germination. This study aimed to evaluate the passage through the digestive system of goats and sheep at different ingestion periods, and its effect on recovery, quality and anatomical aspects of M. tenuiflora seeds. For this experiment, 24 animals (12 goats and 12 sheep) were confined, and 400 seeds were provided for each animal. After the seeds supply the seeds present in the feces of the animal were periodically collected. The study was performed in a completely randomized design, in a factorial scheme 2 × 5 + 1, with two animal species (goat and sheep), five periods of seed excretion (24, 48, 72, 96, and 120 h) plus a control treatment composed of intact seeds. Recovery, seed quality, anatomy, and seedling vigor were evaluated. M. tenuiflora seeds were physically characterized as small and tended to pass quickly through the animal's digestive system. The seed recovery occurs up to 120 h for goats and 96 h for sheep after being provided, and the highest number of seeds were recovered after 48 h. The seeds collected after 120 h from goats and after 24 and 96 h from sheep showed improved germination and overcoming of seed dormancy. The anatomy analysis demonstrates that the passage through the animal digestive system promotes scarification of the seed tegument.

Effects of caatinga propolis from Mimosa tenuiflora and its constituents (santin, sakuranetin and kaempferide) on human immune cells

Ethnopharmacological relevancePropolis is a bee product used in folk medicine to treat inflammatory diseases. Diverse types of propolis are produced worldwide depending on the local flora. Recently, research has been focused on a propolis sample produced in the northeast Brazilian “caatinga” from Mimosa tenuiflora, popularly known as “jurema-preta”. Aim of the studyA possible immunomodulatory/anti-inflammatory action was analyzed to verify the potential of M. tenuiflora propolis (MP) and its constituents (santin, sakuranetin and kaempferide) in human immune cells under baseline conditions or in LPS-stimulated cells. MethodsCell viability, cytokine (TNF-α, IL-1β, IL-6, IL-8, IL-10) production and intracellular pathways (NF-kB and p38 MAPK) were evaluated. ResultsUnder basal conditions, MP and sakuranetin did not affect cytokine production; santin enhanced TNF-α, IL-1β, IL-6, while kaempferide inhibited IL-8 and IL-10. In LPS-stimulated cells, MP and its compounds exerted an inhibitory activity on TNF-α and IL-1β, while no effects were seen on IL-6 and IL-8. Santin and kaempferide inhibited IL-10 production. No significant differences were seen on NF-kB and p38 MAPK intracellular pathways. ConclusionData indicated the immunomodulatory action of caatinga propolis and its constituents at noncytotoxic concentrations, specifically an anti-inflammatory activity in LPS-treated cells by inhibiting cytokine production. Santin, sakuranetin and kaempferide appeared to be involved in MP activities.

Combination of organic amendments as a soil recovering strategy at a desertification hotspot in the Brazilian semiarid

AbstractThe use of manure, organic mulch, and legume‐based fertilizers can increase soil fertility in semiarid regions. However, the effects of such practices on soils prone to desertification are unknown. This study aimed to evaluate soil fertility and herbaceous stratum yield on soils amended with different organic inputs in the desertification hotspot of Irauçuba, Ceará state, Brazil. The experiments were performed in two areas with different soils (Planosol and Cambisol), in randomized blocks with a 2 × 2 × 3 + 1 factorial design. The factors consisted of (i) mulch (presence or absence of Copernicia prunifera [carnauba palm] straw), (ii) manure (sheep or cattle), and (iii) legume‐based fertilizer (Mimosa tenuiflora [jurema], Mimosa caesalpiniifolia [sabiá], and Leucaena leucocephala [leucena]), in addition to an untreated control, with three replicates. Soil chemical attributes and herbaceous stratum yield were assessed in the two areas. Positive effects of the combined use of carnauba palm straw, manures, and legume‐based fertilizers on soil fertility were more evident for Planosol than for Cambisol. The combination of sheep manure with sabiá‐based fertilizer in Planosol, and cattle manure and leucena‐based fertilizer in Cambisol increased herbaceous stratum yield. Herbaceous stratum yield in Planosol is more responsive to the input of great amounts of soil nutrients (especially P) and it increases soil organic matter levels, while in Cambisol, herbaceous stratum yield is more associated with the ability of inputs to make nutrients readily available. The use of organic amendments can reverse, in the short‐term, degradation and desertification processes of soils in the Brazilian semiarid region.