Mimosa Tenuiflora Research Articles

Evaluation of the Chemical Profile and Antioxidant Capacity of Green, Brown, and Dark Propolis.

The chemical composition of propolis varies between different types, due to the specific vegetation found near the hives and the climatic and soil conditions worldwide. Green propolis is exclusive to Brazil, produced by bees, with the resin of the plant Baccharis dracunculifolia. Brown propolis is a specific variety produced mainly in Northeast Brazil from the plant Hyptis divaricata, also known as "maria miraculosa". Dark propolis is a variety of propolis produced by bees from the resin of the plant known as Jurema Preta (Mimosa hostilis benth). In this study, the aqueous extracts of green, brown, and dark propolis were analyzed for their antioxidant capacity using ABTS, FRAP, and DPPH, and their chemical profiles were determined using paper spray mass spectrometry. Among the three extracts, green propolis had the highest content of total phenolic compounds (2741.71 ± 49.53 mg GAE. 100 g-1), followed by brown propolis (1191.55 ± 36.79 mg GAE. 100 g-1), and dark propolis had the lowest content (901.79 ± 27.80 mg GAE. 100 g-1). The three types of propolis showed high antioxidant capacity, with green showing the highest antioxidant capacity for the three methods used. Using paper spray mass spectrometry, it was possible to suggest the presence of 116 substances, including flavonoids (56), phenylpropanoids (30), terpenes (25), carboxylic acids (1), benzoic acid derivatives (1), fatty acids (1), amino acids (1) and alkaloids (1). The compounds in the green, brown, and dark propolis extracts reinforce the bioactive potential for application in these tree extracts' food and pharmaceutical products.

Effects of Increasing Levels of Total Tannins on Intake, Digestibility, and Balance of Nitrogen, Water, and Energy in Hair Lambs.

This study aims to evaluate the effects of increasing tannin levels from Mimosa tenuiflora hay on the intake, digestibility, and balance of nitrogen (N), water, and energy in hair lambs. Thirty-two Santa Ines lambs, at an average age of 150 days and body weight of 26.75 ± 2.29 kg, were randomly assigned to four treatments in a completely randomized design. The treatments consisted of four diets: a control diet, tannin-free, and three diets with increasing levels of total tannin, 26.2, 52.4, and 78.6 g tannin/kg dry matter (DM). Including the total tannins in the lambs' diet led to a quadratic increase in the intake of nutrients, N-retention (g/day), water intake, water absorption and retention, energy intake, and energy excretion in feces and gases. However, the digestibility of crude protein, neutral and acid detergent fibers, and total carbohydrates decreased. It was observed that there is a correlation between the variable nutrient digestibility and N-ingested and the N-absorbed, N-urinary, and N-retained. However, the N-excreted in feces did not correlate with any of the variables studied. It is recommended to include 33 g/kg DM of total natural tannins from Mimosa tenuiflora hay in the diet of hair lambs, as it improves intake, energy balance, dietary N, and body water composition while reducing the excretion of N-urinary and gas emissions to the environment.

Biochemical characterization and mycorrhizal fungal community of plant species in the Brazilian seasonal dry forest.

Invasive alien plant species (IAPS) have the ability to change the biochemical properties and the arbuscular mycorrhizal fungal (AMF) community structure in their rhizosphere. Organic acids, microbial activity, and AMF play a key role in the invader's spread and also has interactions with the soil chemical factors. Our aim here was to assess the rhizosphere's biochemical factors, AMF community composition, and soil chemical properties associated with Cryptostegia madagascariensis (IAPS) and Mimosa tenuiflora (endemic plant species) from the Brazilian Seasonal Dry Forest. The highest values of total glomalin (5.87 mg g-1 soil), root colonization (54.5%), oxalic and malic acids (84.21 and 3.01 μmol g-1 , respectively), microbial biomass C (mg kg-1 ), Na+ (0.080 cmolc kg-1 ), Ca2+ (7.04 cmolc kg-1 ), and soil organic carbon (4.59 g kg-1 ) were found in the rhizosphere of C. madagascariensis. We found dissimilarities on AMF community structure considering the studied plant species: (i) Racocetra coralloidea, Dentiscutata heterogama, Dentiscutata cerradensis, Gigaspora decipiens, and AMF's richness were highly correlated with the rhizosphere of M. tenuiflora; and (ii). The rhizosphere of C. madagascariensis was highly correlated with the abundance of Claroideoglomus etunicatum, Rhizoglomus aggregatum, Funneliformis mosseae, and Funneliformis geosporum. The results of our study highlight the importance of considering C. madagascariensis as potential hosts for AMF species from Glomerales, and a potential plant species that increase the bioavailability of exchangeable Na and Ca at semi-arid conditions.

Seed recovery of native plant species of the Caatinga biome ingested by goats and its effect on seed germination, in Brazilian semiarid region

The plant species of the Caatinga biome are food sources of great importance for herds. Goat farming is one of the most explored agricultural activities in the Northeast region of Brazil, where goats are important seed dispersers in semiarid areas. In this study, we evaluate the passage of seeds of four Caatinga forest species through goats’ digestive system, their recovery, and the effect on seed germination (Ger). A completely randomized experimental design was used. The seeds of four species Piptadenia stipulacea, Mimosa tenuiflora, Desmanthus virgatus, and Mimosa caesalpiniifolia were provided to eight goats and evaluated after nine excretion periods (12, 24, 36, 48, 60, 72, 84, 96, and 108 h). Seed recovery was low, with values of 10.87% for M. tenuiflora, 3.41% for P. stipulacea, 2.04% for M. caesalpiniifolia, and 1.54% for D. virgatus. The seeds of D. virgatus and M. tenuiflora are small and pass faster through goats’ digestive system, with the largest amount recovered 48 h after ingestion. In contrast, P. stipulacea and M. caesalpiniifolia seeds are retained for a longer period in the intestine of the animals, with the highest number obtained 72 and 96 h after, respectively. The passage of seeds through the digestive system of D. virgatus for 24 h and P. stipulacea for 72 h, promoted the overcoming of dormancy, reaching Ger values of 66.7 and 35.7%, respectively. The Ger of M. tenuiflora seeds as well as the Ger and survival of M. caesalpiniifolia seeds decreased after passing through the digestive system. The recovery of seeds from the four species indicates that goats are efficient seed dispersers in Caatinga areas.

Can the traditional use of native plant species in rural communities in the Brazilian semi-arid region be affected by global warming?

Extreme climate change events are capable of modifying the physiognomy of landscapes, impacting millions of people around the world. Consequently, the traditional knowledge of people residing in these regions about local natural resources may also be affected. To identify how the traditional use of native plant species can be influenced by a change in the availability of these species in a rural community in a semi-arid region, in a scenario of climatic extremes, we developed a Pressure Indicator for Use Preference (PIUP), seeking to identify the species under the greatest pressure of use. The study was carried out in the São Francisco Rural Community, in the Cabaceiras Municipality, in the semi-arid region of the Paraíba State, with 42 local informants. The species with the highest PIUP had their potential distribution for the year 2050 modeled using the HadGEM2-ES climate model under the RCP4.5 scenario, as an optimistic forecast, and the RCP8.5 scenario, as a pessimistic forecast. The construction of the models identified a potential increase in the coverage area of ​​all analyzed species, with a greater territorial extension for the RCP8.5 scenario. Myracrodoun urundeuva, Mimosa tenuiflora and Croton blanchetianus were the species with the lowest potential area growth for the year 2050. The high use of species, especially M. urundeuva, associated with reduced growth in a more arid environment is a worrying factor for the population structure of the species, as well as for rural communities that make representative use of the species.

Distribution of thornless Mimosa tenuiflora, Piptadenia stipulacea and Cnidoscolus quercifolius in a seasonally dry tropical forest remnant

ABSTRACT The density and distribution of three tree species of local economic importance with, but sometimes without, thorns (Mimosa tenuiflora, Piptadenia stipulacea) or urticating hairs (Cnidoscolus quercifolius) thriving in native seasonally dry tropical forests, were assessed in a 43.5-hectare cattle-grazed caatinga remnant, located in Patos/Paraíba, Brazil. We recorded and georeferenced all the thorny and thornless regenerant plants (≥50 cm high and circumference at breast height (CBH) < 6 cm). We also recorded and georeferenced all the mature thorny and thornless trees (CBH ≥ 6 cm); in addition all the thornless mature trees and their respective nearest thorny mature plant were measured. Thornless M. tenuiflora, P. stipulacea and C. quercifolius totaled 560, 9 and 13 plants, respectively. The thornless plants formed groups and the mature ones showed height and CBH similar to their respective nearest mature thorny neighbor. Based on our data and on published data, we suggest management practices aiming at replacing thorny trees by thornless trees, including among others: the planting of 15 groups/ha of, respectively, 7, 2 and 2 thornless M. tenuiflora, P. stipulacea and C. quercifolius seedlings. Such a progressive replacement of the thorny trees by thornless trees would facilitate grazing and the management of the forest.

Evaluation of pH-sensitive nanocontainers composed of hierarchical ZnO mesoporous structures loaded with the Mimosa tenuiflora extract for applications in a saline solution

Evaluation of pH-sensitive nanocontainers composed of hierarchical ZnO mesoporous structures loaded with the Mimosa tenuiflora extract for applications in a saline solution

In Vitro Biological Activity and Lymphoma Cell Growth Inhibition by Selected Mexican Medicinal Plants.

Cancer is a major health problem with significant morbidity and mortality. In addition, plants are a source of metabolites with diverse biological properties, including antitumor potential. In this study, we investigated the in vitro murine lymphoma L5178Y-R cell growth inhibition, human peripheral blood mononuclear cells (PBMC) toxicity and proliferation, and antioxidant, hemolytic, and anti-hemolytic activities of methanol extracts from 15 plants of traditional use in Mexico. Justicia spicigera caused the highest tumor cell growth inhibition with a half maximal inhibitory concentration (IC50) of 29.10 µg/mL and a selectivity index >34.36 compared with those of PBMC, whereas Mimosa tenuiflora showed the highest lymphoproliferative activity from 200 µg/mL compared with that induced by concanavalin A. In addition, M. tenuiflora showed an antioxidant effect (IC50 = 2.86 µg/mL) higher than that of ascorbic acid. Regarding the hemolytic and anti-hemolytic activity, all extracts presented significant anti-hemolytic activity. The extract of J. spicigera is emerging as a possible source of effective antineoplastic compounds.

Can phytogenic additives replace monensin sodium in beef cattle feeding?

Monensin sodium is one of the most common ionophores used in livestock feeding; however, ionophores are condemned by organized consumer groups. Bioactive compounds from plants found in the seasonally dry tropical forest have similar mechanisms of action as ionophores. The aim was to investigate the effects of replacing monensin sodium with phytogenic additives on the nutritional efficiency of beef cattle. Five 14-month-old Nellore bulls (average body weight 452.68 ± 42.60kg) were used in the study. The experiment was designed as a 5 × 5 Latin Square (five treatments and five 22-day experimental periods). Within each period, 15days were used for adaptation of animals to experimental conditions and 7days for data collection. Bulls were fed a control diet (without additives), monensin (a diet containing monensin sodium 40% as a synthetic additive), and three diets containing phytogenic additives prepared from Anadenanthera macrocarpa (Benth) Brenan, Mimosa tenuiflora (Willd) Poiret, or Prosopis juliflora (Sw.) DC. Nutritional efficiency was assessed through feed intake, nutrient digestibility, feeding behavior, and hematological parameters. Monensin and phytogenic additives did not influence (P > 0.05) feeding behavior or hematological parameters, but the nutrient intake was highest for bulls supplemented phytogenic additives (P < 0.05). Monensin supplementation did not influence (P > 0.05) feed intake. The phytogenic additives and monensin sodium increased (P < 0.05) the nutrient digestibility. Therefore, the phytogenic additives from P. juliflora, A. macrocarpa, and M. tenuiflora can be recommended to enhance the nutritional efficiency of confined Nellore cattle.

Preliminary evaluation of haemostatic and wound healing potential of Heliotropium europaeum.

Heliotropium europaeum has been traditionally used to stop bleeding and accelerate scarring. This study provides a scientific evaluation of H. europaeum haemostatic and healing potential. To evaluate the haemostatic effect of H. europaeum, the time of bleeding of fresh wounds induced experimentally in rats was studied. Excision wounds were induced upon four groups; each one contains sixrats to estimate the healing properties of wounds. Group 1 was assigned as control (not treated), group 2 was daily treated with H. europaeum leaf powder, group 3 was treated with H. europaeumevery 6days and group 4 was treated with a reference drug, an emulsion containing 10% of Mimosa tenuiflora extract. All the parameters were significantly tested (p < 0.05) with comparison to a group control. The use of H. europaeum significantly shortened the bleeding time. The rats which were daily treated with H. europaeum healed in 12days. This time period was significantly shorter than the control groups. Wound excision was uniformly induced randomly on the dorsum of rats in 4 groups (tested support and control). The post-healing biopsies were histologically assessed, revealed a better healing quality, and continued complete tissue regeneration, abundant and well-organized network of collagen fibres, and low numbers of inflammatory cells. The experimental data revealed that H. europaeum displayed remarkable haemostatic and wound healing activities.

Gas exchange and initial growth of Mimosa tenuiflora (Willd.) Poir. plants under salinity conditions

This work aimed to evaluate the physiological behavior and growth of jurema-preta seedlings under salinity conditions. The experiment was conducted at the Forestry Nursery of the Academic Unit of Forestry Engineering/UFCG, with the plants kept in 'Leonard' pots. The salinity treatments were distributed in a completely randomized design, with five levels of electrical conductivity (EC) of the nutrient solution (0.5; 2.0; 3.5; 5.0 and 6.5 dS m-1) and four repetitions. The salinization of the solution was achieved by adding a mixture of NaCl, CaCl2.2H2O and MgCl2.6H2O salts. There was a progressive decrease in plant height, stem diameter, relative growth rate, relative water content, stomatal conductance, transpiration rate, photosynthesis rate in the dry mass production of the plants as the EC of the solution increased. Transpiration was less sensitive to salinity than stomatal conductance and seedling photosynthesis. Among the growth parameters, the roots were the most affected by salinity.

Chemical composition and energy potential of woody species in dry forest: subsidies for sustainable forest management

Energy demand, especially in developing countries, is partly supplied by firewood and charcoal from natural forests. However, there are not always previous studies of energy quality, with implications for forest management. This study aimed to characterize the energy potential of the wood of six shrub-tree species from the Caatinga and the influence of the circumference class on their chemical properties and energy potential to subsidize forest management. Thus, Anadenanthera colubrina var. cebil (Angico), Cenostigma bracteosum (Catingueira), Cnidoscolus quercifolius (Faveleira), Mimosa ophthalmocentra (Jurema branca), Mimosa tenuiflora (Jurema preta) and Aspidosperma pyrifolium (Pereiro) from an area of ??Dry Forest (Caatinga) in Floresta, Pernambuco state, Brazil, were analyzed. Each species had three individuals sampled in five circumference classes (I a V) at 1.30 m from the ground. Carbon, hydrogen, nitrogen, insoluble lignin, and ash contents, as well as higher and lower calorific values, apparent and energetic densities and energy production were evaluated. Then, a completely randomized design in a factorial arrangement (species x circumference classes) with three replications, and comparison of means (Tukey’s test, p &lt; 0.05) were considered in the statistical analysis. A Cluster analysis was also performed aiming at joint analysis of variables. The results showed that the biomass of M. ophthalmocentra and M. tenuiflora have the highest energy density and amount of energy per unit of mass. C. quercifolius and A. pyrifolium were lower when compared to the other species. In addition, M. ophthalmocentra, M. tenuiflora, A. colubrina and C. bracteosum stood out for energy generation. Classes IV and V can be indicated as biomass for combustion, while class I has disadvantages due to higher nitrogen content and lower upper and lower calorific values.

In vitro evaluation of biological fungicides against Curvularia eragrostidis, a phytopathogenic fungus of pineapple crop

In vitro effectiveness of two plant extract-based products, System Max® (Mimosa tenuiflora + Quercus robur) and Sedric 4X® (Yucca schidigera); and three biological control agents-based products, BliteFree® (Streptomyces spp.), BioFungus Clean® (Bacillus subtilis + B. thuringiensis + Trichoderma harzianum + T. viride), and Tonka® (Bacillus subtilis + Trichoderma harzianum + Streptomyces lydicus) were evaluated against Curvularia eragrostidis, an associated fungus to leaf spot disease in pineapple (Ananas comosus) plants. The strain was identified by phylogenetic reconstruction using the rDNA ITS1-5.8S-ITS2 sequences as Curvularia eragrostidis. Through a doses response-bioassays f ive concentrations: 0 (control), 0.125, 0.25, 0.5 and 1.0% of each commercial product were tested. Daily mycelial growth (DMG), mycelial growth inhibition (% MGI) and the lethal mean concentration (LC50 ) were calculated. Sedric 4X® showed the highest% MGI (75%) at highest concentration, while Tonka® showed the highest% MGI (65%) at lower concentration, being constant (65.3-66.9%) at all the concentrations tested. The lowers values of EC50 were achieved by Tonka® (0.008%), BliteFree® (0.015%) and BioFungus Clean® (0.017%). In contrast, the highest CE50 were reached by Sedric 4X® (0.260%) and System Max® (3.5%). SystemMax® and Sedric 4X®, showed a positive relation between concentration and% MGI, despite its highest CE50 values. The biofungicides seem to be more effective against C. eragrostidis than plant extracts fungicides.

Genetic diversity of Mimosa tenuiflora (Willd.) Poir.: an intensively exploited wood tree in the Brazilian tropical semi-arid vegetation

Genetic diversity of Mimosa tenuiflora (Willd.) Poir.: an intensively exploited wood tree in the Brazilian tropical semi-arid vegetation

Antioxidant, anti-inflammatory and analgesic activity of Mimosa acutistipula (Mart.) Benth

Ethnopharmacological relevanceMedicinal plants belonging to the genus Mimosa, such as Mimosa tenuiflora, M. caesalpinifolia, and M. verrucosa are known for their popular use for asthma, bronchitis and fever. Ethnopharmacological studies report that Mimosa acutistipula is used to treat alopecia and pharyngitis, conditions that can be related to oxidative stress, inflammatory processes and painful limitations. However, there is no studies on its efficacy and mechanism of action. Aim of the studyTo elucidate the antioxidant, anti-inflammatory, analgesic and antipyretic activity of M. acutistipula leaves. Materials and methodsPhytochemical profile of M. acutistipula extracts was evaluated by several reaction-specific methods. Secondary metabolites such as tannins, phenols and flavonoids were quantified with colorimetric assays. In vitro antioxidant potential was evaluated using DPPH and ABTS + as free radical scavenging tests, FRAP and phosphomolybdenum as oxide-reduction assays, and anti-hemolytic for lipid peroxidation evaluation. In vivo anti-inflammatory evaluation was performed by paw edema, and peritonitis induced by carrageenan. Analgesic effect and its possible mechanisms were determined by acetic acid–induced abdominal writhing and the formalin test. Antipyretic activity was evaluated by yeast-induced fever. ResultsCyclohexane, chloroform, ethyl acetate and methanol extracts of leaves had presence of tannins, flavonoids, phenol, alkaloids, terpenes (except methanolic extract), and saponins (only for methanolic and chloroformic extracts). In phenols, flavonoids and tannins quantification, methanolic and ethyl acetate extract had higher amounts of this phytocompounds. Ethyl acetate extract, due to its more expressive quantity of phenols and flavonoids, was chosen for carrying out the in vivo tests. Due to the relationship between oxidative stress and inflammation, antioxidant tests were performed, showing that ethyl acetate extract had a high total antioxidant activity (70.18%), moderate activity in DPPH radical scavenging, and a moderate ABTS + radical inhibition (33.61%), and FRAP assay (112.32 μg Fe2+/g). M. acutistipula showed anti-inflammatory activity, with 54.43% of reduction in paw edema (50 mg/kg) when compared to the vehicle. In peritonitis test, a reduction in the concentration of NO could be seen, which is highly involved in the anti-inflammatory activity and is responsible for the increase in permeability. In the analgesic evaluation, most significant results in writhing test were seen at 100 mg/kg, with a 34.7% reduction of writhing. A dual mechanism of action was confirmed with the formalin test, both neurogenic and inflammatory pain were reduced, with a mechanism via opioid route. In the antipyretic test, results were significantly decreased at all concentrations tested. ConclusionM. acutistipula leaves ethyl acetate extract showed expressive concentrations of phenolic compounds and antioxidant activity. It also exhibited anti-inflammatory and analgesic activity, besides its antipyretic effect. Thus, these results provide information regarding its popular use and might help future therapeutics involving this specimen.

Statistics-Based Optimization of the Extraction Process of N,N-dimethyltryptamine from Mimosa tenuiflora Stem Bark

Statistics-Based Optimization of the Extraction Process of N,N-dimethyltryptamine from Mimosa tenuiflora Stem Bark

Briquette production as a sustainable alternative for waste management in the tannin extraction industry.

Tannins are polyphenols that can be extracted from different parts of the plant and have different known commercial applications. The extraction of tannins generates a significant amount of low-density wastes. An alternative that can be applied to ensure energy reuse of this low-density waste is a densification process, such as briquetting. Thus, this research work aimed to assess the energy potential of the residue from the extraction of tannins of different forest species, aiming at its energy reuse in the form of briquettes. Stem barks of 6 forest species (Acacia mangium, AM; Anacardium occidentale, AO; Anadenanthera colubrina, AC; Azadirachta indica, AI; Mimosa caesalpiniaefolia, MC; Mimosa tenuiflora, MT) were used after tannin extraction. Bulk density, energy (higher, lower, and net heating value) and thermal (TG/DTG) properties, and chemical constitution (extractives, lignin, and holocellulose) of the materials were determined. In addition, briquette compaction ratio, apparent density, volumetric expansion, and water adsorption over the days were evaluated. The highest bulk densities were found in the barks of Anadenanthera colubrina (0.529g.cm-3) and Mimosa tenuiflora (0.407g.cm-3), whereas the species that showed the best result of higher heating value was Acacia mangium (20.44MJ.kg-1), followed by Azadirachta indica (19.39MJ.kg-1) and Mimosa caesalpiniifolia (18.85MJ.kg-1). Briquetting increased the density of the evaluated material by 2.3 to 4.9 times. All briquettes produced with wastes from tannin extraction evaluated in this work showed potential for energy production. With more information on the quantification of waste generated and data on the economic viability of production, these tannin-producing industries can benefit both environmentally and economically, by reusing these wastes for energy production.

Tannin-rich tree bark extracts inhibit the development of bacteria associated with bovine mastitis

Condensed tannins were quantified in the barks of selected tree species, and the antibacterial activity of these substances against clinical and subclinical isolates of bovine mastitis were evaluated. Tree barks from Mimosa tenuiflora, Mimosa caesalpiniifolia, Anacardium occidentale, and Stryphnodendron adstringens were used, as well as commercial tannin from Acacia mearnsii. The tannins were extracted using a mixture of ethyl alcohol and acetone. The moisture content (MC), Stiasny index (SI), total solids content (TSC), and condensed tannins (CT) were measured. The evaluation of antimicrobial activity was determined by applying the disk diffusion test. The species that showed the highest CT were M. tenuiflora and A. occidentale, with 37.3% and 37.3%, respectively. The highest SI were obtained by the species A. occidentale and M. caesalpiniifolia, with values above 90%. The bacterium with the lowest resistance to the use of tannins was Streptococcus uberis. A. mearnsii was the only one capable of inhibiting the growth of Escherichia coli, with a mean inhibition halo of 9 mm. All species under study showed high values of condensed tannins in their barks. In addition to showing good performance as antimicrobial agents, these tannins indicated potential applications in the development of natural medicines for the treatment of bovine mastitis.

Rhizobacteria from Root Nodules of Brazilian Native Legumes Promote-Growth Sorghum bicolor L. Under Drought Conditions

ABSTRACT Plant growth-promoting bacteria (PGPB) are bacteria that can enhance protect native plants from abiotic stresses including drought. The inoculation of these PGPB into crop forage plants may increase productivity helping to suppress its growing demand in semiarid regions. The present paper aims to evaluate the Sorghum bicolor L. morphogenic characteristics and growth inoculated with native bacterial isolates from the Brazilian semiarid region under drought conditions. The endophytic bacteria isolated from root nodules of native leguminous plants named Mimosa tenuiflora (isolates – MTBV77, MTP78, MTBV12), Desmanthus pernambucanus (isolates – DPP1) and Mimosa caesalpiniifolia (isolate – MCLR34) were all presumably identified as rhizobia by performing the Polymerase Chain Reaction duplex amplification technique using primers that amplify the nifH and nodC genes. The seeds and roots of sorghum seedlings were inoculated with bacterial isolates. The plants were cultivated in pots under favorable water conditions (80% of the available water) and dry conditions (40% of the available water). The inoculation with bacterial isolates increased emission, elongation and length of the S. bicolor leaves, as well as increased its stem elongation, height, and shoot dry-matter content. However, the magnitude of the effects varied according to the imposed water regime. Thus, rhizobacteria from root nodules of Brazilian native legumes have the potential to promote plant growth. The bacterial isolates DPP1 and MTBV77 attenuate the effects of water stress and promote S. bicolor growth under drought conditions and are therefore promising for the use as an inoculant for S. bicolor sustainable cultivation to forage purposes.

Efficiency of Pyroligneous Extract from Jurema Preta (Mimosa tenuiflora [Willd.] Poiret) as an Antiseptic in Cats (Felis catus) Subjected to Ovariosalpingohysterectomy.

Simple SummaryThe pyroligneous extract from Mimosa tenuiflora was used as an antiseptic in cats. The objective of this study was to analyze the action of 20% pyroligneous extract from M. tenuiflora as an antiseptic for surgical wounds of cats subjected to ovariohysterectomy. In addition to the absence of cytotoxicity, the number of bacteria was reduced after treatment. Tissue dehiscence, hyperemia, edema, crust, and secretions were not observed. Thus, the pyroligneous extract of M. tenuiflora has potential as a natural antiseptic alternative.Pyroligneous extract of Jurema preta (Mimosa tenuiflora [Willd.] Poiret) was evaluated for its efficacy as a cutaneous antiseptic in cats (Felis catus) that were subjected to ovariosalpingohysterectomy. For this purpose, 30 cats without a defined breed were sterilized and divided into two groups. The first group was the positive control, treated with 0.5% chlorhexidine-alcohol solution, and the second group was treated with 20% pyroligneous extract of M. tenuiflora. Regardless of age and sex, all animals had visible healing at similar times. A significant reduction in bacterial growth was observed in animals treated with the extract, and no cytotoxicity was observed in the feline epithelial cells. In addition, surgical wounds of cats treated with M. tenuiflora extract exhibited improved healing. On agar plates, treatment with both chlorhexidine and M. tenuiflora extract resulted in the inhibition zones for all bacterial strains isolated from surgical wounds. Therefore, M. tenuiflora extract is demonstrated to have antiseptic effects on the surgical wounds of cats undergoing ovariosalpingohysterectomy.