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Articles published on mimosa-pigra

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  • Open Access Icon
  • Research Article
  • Cite Count Icon 1
  • 10.21829/abm129.2022.1923
Hacia la restauración con Annona glabra (Annonaceae) de una selva inundable: establecimiento y crecimiento de plántulas, y cambio en la vegetación acompañante
  • Aug 26, 2022
  • Acta Botanica Mexicana
  • Edgar Abel Sánchez-García + 4 more

Antecedentes y Objetivos: Actualmente existe deterioro y disminución de selvas inundables debido a la deforestación para ganadería e introducción de pastos exóticos. Los objetivos del presente estudio fueron evaluar el porcentaje de supervivencia de plántulas de Annona glabra, el cambio en la vegetación acompañante, y estimar los costos de los tratamientos experimentales de restauración ecológica de una selva inundable de A. glabra transformada en pastizal e invadida por el pasto exótico Echinochloa pyramidalis. Métodos: El estudio se realizó en un pastizal inundable en la costa central de Veracruz, México. Consistió en sembrar cuatro plántulas de A. glabra en cuadros experimentales expuestos a distintas técnicas de restauración. Se probaron 15 tratamientos experimentales que consistieron en cinco técnicas de restauración (sin modificación, cubierta plástica, plantación de Pontederia sagittata, remoción de suelo, y elevación del suelo) y tres pretratamientos aplicados a las plántulas sembradas (plántulas de vivero con y sin fertilizante, plántulas de selva) en un diseño de bloques al azar.Resultados clave: Se registró baja supervivencia de plántulas (30.7%), siendo mayor en plántulas de selva (41.1%). El mayor porcentaje de supervivencia se registró con la técnica de elevación del suelo, seguido de la de remoción. Se registraron 40 especies en la vegetación; la mayor riqueza específica se observó en las técnicas de elevación del nivel del suelo y remoción. Las especies con mayor Valor de Importancia Relativa fueron Echinochloa pyramidalis, Mimosa pigra y Annona glabra. El pretratamiento en el cual se obtuvieron las plántulas directamente de la selva fue el más económico.Conclusiones: Se requieren acciones para incrementar el éxito de la reforestación. Para futuros proyectos de restauración, recomendamos la elevación del suelo en áreas de transplante y cubierta de plástico previo a la plantación, para incrementar la probabilidad de supervivencia, y reducir la cobertura del pasto; además de la reforestación con plántulas colectadas de un fragmento de selva.

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  • Research Article
  • Cite Count Icon 6
  • 10.1080/23802359.2022.2086076
The complete chloroplast genome of Mimosa pigra L. (Fabaceae), a notorious invasive plant
  • Jun 3, 2022
  • Mitochondrial DNA Part B
  • Nguyen Pham Anh Thi + 4 more

Mimosa pigra L., also called the giant sensitive tree, is native to tropical America and has invaded Africa, Asia, and Australia. Here, we report the complete chloroplast genome of M. pigra, which was 165,996 bp in length and composed of a large single-copy region (LSC; 93,299 bp), a small single-copy region (SSC; 17,989 bp) and two inverted repeat regions (IRs; 27,354 bp). The complete M. pigra chloroplast genome included 83 protein-coding genes, 37 tRNAs and 8 rRNAs. Phylogenetic analysis using the maximum likelihood method revealed the monophyly of M. pigra and related taxa of the subfamily Caesalpinioideae. In comparison, the members of Papilionoideae were paraphyletic.

  • Research Article
  • Cite Count Icon 51
  • 10.1016/j.envres.2022.113073
Amino-functionalized biochars for the detoxification and removal of hexavalent chromium in aqueous media
  • Mar 11, 2022
  • Environmental Research
  • Anusha Ekanayake + 3 more

Amino-functionalized biochars for the detoxification and removal of hexavalent chromium in aqueous media

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  • Research Article
  • Cite Count Icon 3
  • 10.24925/turjaf.v10i2.203-210.4537
Melissopalynological Characterization of Honey Samples from Southeastern, Nigeria
  • Mar 2, 2022
  • Turkish Journal of Agriculture - Food Science and Technology
  • Nchedochukwu Clara Ikegbunam + 4 more

The identification of plants in which the bees forage is key in establishing bee farms and increasing honey production. In this study pollen analysis of honey samples from the southeastern part of Nigeria was carried out to ascertain their floral sources and ecological origin. The honey samples were acetolyzed and microscopically studied to determine the pollen types. A total of seventy-one pollen types belonging to forty-one families of plants were identified. The honey samples were dominated by pollen grains from the families of Arecaceae, Euphorbiaceae, Myrtaceae, Irvigiaceae, Fabaceae, Combretaceae/Melastomataceae, and phyllanthaceae. Some of the dominant pollen grain identified include Elaeis guineensis, Alchornea cordifolia, Hymenocardia acida, Ocimum gratissimum, Syzygium guineense, Nauclea latifolia and Afzelia africana. Out of the six samples studied Njikoka sample was monofloral having Mimosa pigra as predominant pollen while Ayamelum, Ekwusigo, Nsukka, Ezeagu, and Udenu samples are multifloral containing Elaeis guineensis, Phyllanthus sp., Piliostigma reticulatum, Irvingia sp., Alchornea cordifolia, and Lannea sp. as major secondary pollen. All the samples analyzed have Elaeis guineensis in common except Ezeagu, indicating that these plants are present in all five locations. These results can also be used as a tool in the geographical identification of Southeastern Nigeria honey from other geopolitical zones.

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  • Research Article
  • 10.1079/pwkb.species.34199
Mimosa pigra (giant sensitive plant)
  • Jan 7, 2022
  • PlantwisePlus Knowledge Bank

Mimosa pigra (giant sensitive plant)

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  • Research Article
  • 10.4038/tare.v24i4.5516
Farmers’ perspective and a geospatial approach on the distribution of <em>Mimosa pigra</em>: a study in Matara District, Sri Lanka
  • Dec 31, 2021
  • Tropical Agricultural Research and Extension
  • P C D Perera + 2 more

Tropical Agricultural Research and Extension is a peer-reviewed international scientific journal covering a wide range of subject areas in tropical and subtropical agriculture published quarterly by the Faculty of Agriculture, University of Ruhuna, Sri Lanka. The journal is also available on the Faculty of Agriculture, University of Ruhuna website https://www.agri.ruh.ac.lk/tare/index.htm .

  • Open Access Icon
  • Research Article
  • 10.13057/biodiv/d221221
Population and distribution of some herons in Babakan Rancabayawak, Bandung City, Indonesia
  • Nov 25, 2021
  • Biodiversitas Journal of Biological Diversity
  • Erri Noviar Megantara + 8 more

Abstract. Megantara EN, Husodo T, Iskandar J, Nurjaman D, Suroso, Aminuddin SF, Atsaury ZIA, Wulandari I, Shanida SS. 2021. Population and distribution of some herons in Babakan Rancabayawak, Bandung City, Indonesia. Biodiversitas 22: 5389-5396. The Cinambo River flood control channel in the Rancabayawak impacts the decline in the habitat quality of some herons, such as cattle egret [Bubulcus ibis (Linnaeus, 1758)], little egret [Egretta garzetta (Linnaeus, 1766)], and Javan pond-heron [Ardeola speciosa (Horsfield, 1821)]. Besides, many built-up areas due to the urbanization in peri-urban areas also impact habitat quality, such as the loss of plants commonly used as perching and nesting sites. Along with the development of Rancabayawak as a built-up area, it is essential to study the management of those birds. This study aims to reveal: 1) plants used as perching and nesting sites, 2) population size and distribution, and management efforts on B. ibis, E. garzetta, and A. speciosa in Babakan Rancabayawak Hamlet, Gedebage Subdistrict, Bandung, West Java, Indonesia. A mixed-methods approach was applied in this study by calculating the estimated population and bird nests, plant inventory survey, and the installation of the A8 mini GPS. Bambusa vulgaris, Leucaena leucocephala, and Mimosa pigra are plants that are used as perching and nesting sites by B. ibis, E. garzetta, and A. speciosa. The estimated population mean of those birds was 630 individuals, 92 individuals, and 43 individuals, respectively, with the population average of those birds, is 1680 individuals. Three hundred twenty-nine nests (329) were found during the study. Based on distribution mapping, birds moved east and south, covering paddy fields in Rancaekek, Solokanjeruk, and Majalaya within a radius of 12 km. Management efforts can be conducted by not cutting down the plants that become nesting and perching sites and maintaining the wetlands in their habitats.

  • Research Article
  • Cite Count Icon 2
  • 10.1016/j.bse.2021.104354
Phytochemical investigation of Mimosa pigra leaves, a sensitive species
  • Oct 27, 2021
  • Biochemical Systematics and Ecology
  • Mohammed F Hawwal + 4 more

Phytochemical investigation of Mimosa pigra leaves, a sensitive species

  • Research Article
  • 10.52714/dthu.10.5.2021.899
Evaluating the invasive status of Mimosa pigra l., Eupatorium odoratum l. and Eichhornia crassipes (mart.) solms in Tram Chim national park in Dong Thap province
  • Oct 10, 2021
  • Dong Thap University Journal of Science
  • Nhung Nguyen Thi Cam + 1 more

The study accessed the threat level of Mimosa pigra, Eupatorium odoratum and Eichhornia crassipes to flora in Tram Chim National Park by using the tour survey and standard quadrat setting method. Regarding canal and swamp habitats, Eichhornia crassipes had a very high threat level (IVI = 155.80% and the level 4 of association), reaching highest point in the rainy season. As regards the inside-dike habitats, Mimosa pigra had a high threat level (IVI = 70.79% and the level 4 of association), reaching the highest point in the dry season. Eupatorium odoratum had a moderate threat level (IVI = 49.99% and the level 3 of association). National Park’ managers enacted some effective controlling and management for Mimosa pigra and Eichhornia crassipes, but not for Eupatorium odoratum due to its low level of encroachment in the wild.

  • Open Access Icon
  • Research Article
  • Cite Count Icon 5
  • 10.1016/j.sajb.2021.03.041
A shotgun proteomic approach reveals protein expression in morphological changes and programmed cell death in Mimosa pigra seedlings after treatment with coumarins
  • Jul 24, 2021
  • South African Journal of Botany
  • Rungcharn Suksungworn + 3 more

Mimosa pigra L. (Fabaceae), commonly known as giant mimosa, is one of the major invasive species, constituting a serious threat to agricultural crops and other plants in the biodiversity. With this study, we aimed to investigate the mechanism underlying the effects on plant growth and development upon exposure to two coumarins. Protein solutions were obtained from giant mimosa seedlings that had been exposed to the coumarins, and they were analyzed with the shotgun proteomic method by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The proteins were identified in seedlings treated with coumarins. The control plants expressed 621 proteins, the plants treated with isoscopoletin expressed 613 proteins, and the plants treated with umbelliferone expressed 640 proteins. The classification of proteins by PANTHER software found that the coumarins interfered with proteins involved in cellular component organization or biogenesis and metabolic processes. STITCH analysis of protein expression after treatment with coumarins confirmed that CUL4, ESF, LNG2 and SCD2 played a role in plant growth and development. WAK1 and WAT1 were involved in cell wall and cell surface responses. At3g61030 and CIP111 were related to Ca2+ accumulation and CAND1, CUL4 and ML5 were linked with programmed cell death. It is worthwhile to mention that coumarins increased morphological changes in the epidermis, Ca2+ accumulation in the roots and protein expression in plant growth and development, morphological changes, Ca2+ accumulation as well as in programmed cell death.

  • Open Access Icon
  • Research Article
  • Cite Count Icon 2
  • 10.31130/jst-ud2021-437
Assessment of the current distribution, dispersal trends and impacts of invasive species in Bana - Nui Chua Nature Reserve, Vietnam
  • Jun 30, 2021
  • Journal of Science and Technology Issue on Information and Communications Technology
  • Pham Thi Kim Thoa, Vu Thi Bich Hau, Nguyen Van Hieu Pham

Invasive species threaten the biodiversity and the function of ecosystems. Drone image, satellite images, and image analysis software were used to create the map of invasive distribution and the potential spreading of invasive plants. 13 most invasive plants were identified with 11 species listed as invasive species in Southeast Asia and 5 of them in the 100 world’s invasive species by IUCN. Three species Merremia boisiana (Gagn.) van Ooststr., Ipomoea eberhardtii Gagn, and Mimosa pigra were identified as the species with high-ranking impacts on biodiversity and ecosystem biodiversity in Ba Na - Nui Chua Nature Reserve (BNNR). Ipomoea eberhardtii Gagn shows the highest spreading rate at 0.65 ± 0.06 ha/month, followed by Merremia boisiana (Gagn.) van Ooststr) and Mimosa pigra at 0.12 ± 0.01 ha/month and 0.01 ± 0.001 ha/month respectively. Fresh biomass of Ipomoea eberhardtii Gagn; Merremia boisiana (Gagn.); Mimosa pigra and Sphagnetola trilobata (L.) Pruski in BNNR are 15.67; 14.9; 8.1 and 6.8 ton/ha. The database of invasive plant distribution and potential spreading will be used to monitor strategies and invasive weeds management in BNNR.

  • Open Access Icon
  • Research Article
  • Cite Count Icon 4
  • 10.25073/2588-1094/vnuees.4582
Study on Adsorption of Methylene Blue from Aqueous Solution by Biochar Derived from Mimosa Pigra Plant
  • Jun 26, 2021
  • VNU Journal of Science: Earth and Environmental Sciences
  • Nguyen Xuan Cuong

Biochar from mimosa pigra was studied to remove methylene blue (MB) from aqueous solution. The properties of biochars were determined using Fourier Transform Infrared, scanning electron microscope, and Brunauer–Emmett–Teller. The biochar achieved the yield of 24.62 % at 500 oC pyrolysis. The specific surface area of ​​the biochar is 285.53 m2/g, the total pore size is 0.153 cm3/g and the ash content is 2.79%. The optimal dose of removing MB of the biochar is 5 g/L and the optimal pH is 2 - 10. MB removal reached over 80% in the first 30 min, followed by a stable period of 120 to 360 min reaching over 90% of removal. Maximum adsorption capacity reached 20.18 mg/g at 25 oC. MB adsorption data is suitable for kinetic models in order: Avrami > Elovich > PSO > PFO. The adsorption process may comprise physical and chemical adsorption andmultiple stages.

  • Open Access Icon
  • Research Article
  • Cite Count Icon 2
  • 10.2478/jas-2021-0001
Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities
  • Mar 3, 2021
  • Journal of Apicultural Science
  • Phanthiwa Khongkarat + 3 more

Abstract Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from Apis mellifera, harvested from the flowers of mimosa (Mimosa pigra L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC50) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC50 of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC50 of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC50 of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).

  • Research Article
  • 10.30848/pjb2021-5(32)
Removal of some plant toxic heavy metals from soil using Mimosa pigra L. plant and effect of methanolic extract of Acacia nilotica L. on removing efficacy
  • Mar 1, 2021
  • Pakistan Journal of Botany
  • Usama Mahalel + 3 more

Removal of some plant toxic heavy metals from soil using Mimosa pigra L. plant and effect of methanolic extract of Acacia nilotica L. on removing efficacy

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  • Cite Count Icon 3
  • 10.5296/jas.v9i1.17938
Direct Shear Strength on the São Francisco River Bank, Northeastern Brazil, With or Without Roots of Different Native Species
  • Jan 20, 2021
  • Journal of Agricultural Studies
  • Igor Pinheiro Da Rocha + 5 more

Several plant species have been studied as reinforcement elements against landslides at slopes, either to protect against the splash effect offered by shoots or anchoring the soil by the root system. The objective of this work was to investigate the influence of roots from shrub species over the soil mechanical attributes such as cohesion and angle of internal friction at the São Francisco riverbank, northeastern Brazil. A trench was excavated under the treetop of every shrubby individual, sampling blocks at 0-0.25, 0.25-0.50, 0.50-0.75, and 0.75-1.00 m depths. The moisture contents, particle size, liquidity limits, plasticity and actual specific mass of the samples were determined. The soil physical and mechanical attributes such as initial and final specific weight, initial and final void content, initial and final degree of saturation, shear resistance peaks, cohesion, and internal friction angle were identified through direct, elementary, consolidated, and undrained tests. In the samples with Solanum paniculatum, Mimosa pigra species, the highest values for cohesion were registered for the samples with roots. Sesbania virgata presented the greatest variation in cohesion and internal friction angle among samples with and without roots. The position and root status influenced the results of the direct shear tests.

  • Open Access Icon
  • Research Article
  • Cite Count Icon 7
  • 10.51791/njap.v2i2.2163
The Natural Forages of Northern Nigeria I. Nitrogen and Mineral composition of Grasses and Browse from the Northern Guinea Savanna and Standing Hays from the different Savanna Zones
  • Jan 15, 2021
  • Nigerian Journal of Animal Production
  • M M Kapu

THE mineral status of nine native grass species and ten browse species from the Northern Guinea Savanna Vegetation was investigated together with the mineral Composition of standing hays from Daura and Kano areas of the Sudan Savanna, Bauchi ranch of the sub-Sudan, Samaru (Shika Kadama) of the Northern Guinea Savanna and Jos area of the Plateau vegetation, in Northern Nigeria. All forage types (grasses, browse and standing hays) which were sampled in April of 1975 were analysed for nitrogen (N), Phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sodium (Na), zinc (Zn) and copper (Cu). The grass species included: Andropogon gayanus, A. ascinodis, Brachiaria jubata, Cynodon dactylon, Hyparthelia dissoluta, Pancium phragmitoides, Setaria anceps, Sporobolus pyramidalis and Urelytram muricatum, while the browse consisted of Adenodolichos panicu latus, Annogeissus leocarpus, Briddelia ferruginea, Crossopteryx febrifug, Dichrostachys cenerea (glometata), Khaya senegalensis, Mimosa pigra, Parinari curatellifolis, Psorospermum guineense, and Pterocarpus erinaceus. Both grass and browse species were leafy materials from among those Species considered to be highly consumed by cattle. in general, the average values for Na, P, and Zn in the grasses, browse and standing hays were inadequate for the nutritional needs of cattle one standing hays from all locations contained suboptimum amounts of N, Ca, P, K, Mg, Na and The Cu content of all three forage types was adequate for cattle nutrition. The grass and browse species contained adequate amounts of N, K, Mg and Cu. The trace elements and Ca and Mg tended to be more concentrated in the browse species than in the grass species. The opposite was true of K. Grasses, browse and standing hays contained equal amounts of Na. Similarly, the grass and browse species contained identical amounts of P. Supplementation of mineral concentrates to include Na, P, K, Ca and Zn was suggested.

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  • Research Article
  • 10.17582/journal.jahp/2021/9.4.463.470
Effect of Tannin-rich Mimosa pigra Leaf Meal in Molasses-based Blocks on Feed Intake, Rumen Parameters, and Blood Metabolites of Dairy Heifers
  • Jan 1, 2021
  • Journal of Animal Health and Production
  • Suntorn Wittayakun + 3 more

Effect of Tannin-rich Mimosa pigra Leaf Meal in Molasses-based Blocks on Feed Intake, Rumen Parameters, and Blood Metabolites of Dairy Heifers

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  • Cite Count Icon 4
  • 10.28940/terra.v38i4.603
The potential of Mimosa pigra to restore contaminated soil with anthracene and phenanthrene
  • Oct 11, 2020
  • REVISTA TERRA LATINOAMERICANA
  • Valentín Pérez-Hernández + 5 more

Hydrocarbon contamination cause serious environmental damage and human health problems, therefore, to resolve this problem has been proposed diversely remediation techniques friendly with the environment i.e. bioaugmentation, biostimulation, natural attenuation and phytoremediation. Among options, the phytoremediation causes minimum alterations to soil, low cost, it has proved good eff iciency and gained public acceptation. In the present study, the capacity of Mimosa pigra to restore a contaminated soil with both anthracene and phenanthrene was evaluated, along with the soil physicochemical changes due to both plant and contaminant presence in the soil. A silt-loam contaminated soil with 100 mg kg-1 (dry weight) anthracene and 200 mg kg-1 (dry weight) phenanthrene was used in all three treatments: Contaminated soil with M. pigra (C1), contaminated soil without M. pigra (C2), and not contaminated soil with M. pigra (C0) as control. After 70 days of experimentation both biomass and height of M. pigra was not affected due to soil contaminants. There was no difference among treatments for some physicochemical characteristics e.g. soil pH, electrolytic conductivity, soil-texture, and total nitrogen content throughout the experiment. M. pigra increased the soil water holding capacity after 50 and 70 days of the experiment, however, total organic carbon content was lower after 70 days compared to the control treatment. The higher contaminant removal rate was before 7 days of experimentation for all treatments. Anthracene and phenanthrene content were lower in treatments with the presence of M. pigra: 92% of phenanthrene and 80% of anthracene were removed in C1 while nearly 77% of phenanthrene and 60% of anthracene were removed in C2. This suggest a greater removal of contaminants using M. pigra. Total removal of contaminants was not achieved. However, M. pigra was able to accumulate both compounds, showing the M. pigra capability for bioremediation use in contaminated soils.

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  • Cite Count Icon 9
  • 10.1111/aec.12895
Mimosa pigra in eastern and southern Africa: Distribution and socio‐ecological impacts
  • May 10, 2020
  • Austral Ecology
  • Arne B.R Witt + 5 more

Abstract The semiaquatic weed Mimosa pigra has negative impacts on biodiversity, fishing, crop and livestock production, and tourism in most places where it has been introduced, established and proliferated. Many of the ecological impacts are well known, but its impacts on rural livelihoods are less well documented, especially in Africa. We mapped the distribution of M. pigra in eastern and southern Africa, and then compared that with its potential distribution based on an ecoclimatic niche model. Household interviews were conducted to assess the impacts of this weed on local livelihoods. Mimosa pigra was found to be invasive in western Ethiopia, around the shores of Lake Victoria and Lake Tanganyika, and along the Tanzanian coastline, northern Malawi, parts of Mozambique and along the Kafue River and in the Barotse floodplain on the Zambezi River in Zambia. According to respondents living along the Kafue River floodplains in Zambia, it has a negative impact on biodiversity, wildlife, livestock, crop production, fishing and mobility. Dense stands prevented the movement of people and livestock, limiting access to croplands, grazing lands and fishing areas. Fish catches have been reduced and fishing equipment damaged. All respondents agreed that their livelihood options would be considerably enhanced if M. pigra was removed from the landscape. Based on its current and potential impact, we therefore recommend that an integrated management plan be developed and implemented, including the appropriate use of biological control agents to reduce the negative impacts of the weed.

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  • Cite Count Icon 4
  • 10.1080/14786419.2020.1752209
A new furanochromone from the leaves of Mimosa pigra
  • Apr 19, 2020
  • Natural Product Research
  • Loan Ngoc Phuong Nguyen + 4 more

Phytochemical study on the EtOAc-soluble extract of the leaves of Mimosa pigra led to the isolation of a new furanochromone, 6,8-dihydroxy-2-methyl-9H-furo[3,2-b]chromen-9-one (1), along with four known compounds (2–5). Their structures were elucidated based on the basis of the spectral interpretation. The plausible biosynthesis pathway for the formation of the new furanochromone was proposed. At a concentration of 100 µM, compound 1 showed no cytotoxicity against human MCF-7 breast cancer cell with a cell viability >50%.

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