Nitrogen Migration Research Articles

Effects of Cultivation Years on the Distribution of Nitrogen and Base Cations in 0–7 m Soil Profiles of Plastic-Greenhouse Pepper

To clarify the migration and accumulation of nitrogen (N), magnesium (Mg), calcium (Ca), and potassium (K) in soil profiles of plastic-greenhouse vegetable fields with cultivation years, soil samples from the 0–7 m soil profiles were collected from 10 pepper greenhouses with 10 and 20 years planting history, and parallel soil samples were taken from adjacent wheat-maize fields as controls. The results showed that: (1) Compared with wheat-maize fields, the total N amount in the 0–7 m soil layers from the greenhouses increased by 6.19 ± 1.16 and 9.11 ± 3.43 t ha−1 at 10 and 20 years, respectively, accounting for about 30.4% and 17.5% of the N input. (2) The N amount that entered the environment outside the 0–7 m soil layers were 6.95 t ± 2.76 and 29.10 ± 10.14 t ha−1 after 10 and 20 years of continuous planting, accounting for approximately 34.2% and 55.9% of the N input, respectively. (3) The concentration of water-soluble Ca and Mg in the 0–7 m soil layers increased significantly with cultivation years, and correlated positively with mineral N concentration. (4) Moreover, K mainly accumulates in the form of non-exchangeable K in the surface layers (0–50 cm). Our results demonstrated that huge amounts of N migrate to the deep soil with the extension of cultivation years in plastic-greenhouse pepper production systems, accompanied by significant leaching of Ca and Mg, while K mainly accumulates in the surface layers.

Study on the Influence of Canopy Density on Cycling of Soil Available N in Different Landform of Loess Plateau in China

In this paper, a total of 330 soil samples with 0-100cm soil depth of 66 planted square forest (10*10m) with different canopy density in the Loess Plateau were selected for the determination and analysis of soil N content in different soil layers, and the effects of different canopy density on soil N cycle under different topographic factors of planted forest were studied. The results showed as follows: (1) the migration mechanism of different N forms to the root surface was different, the migration of nitrate nitrogen to the root surface mainly depended on mass flow, there was enrichment phenomenon near the root, ammonium nitrogen mainly through diffusion, resulting in deficiency and loss in the near rhizosphere, and the leaching loss of nitrate nitrogen was affected by soil water and root growth. (2) The thickness, composition and decomposition rate of litter were different due to different canopy density, which affected the content of ammonium nitrogen and nitrate nitrogen in forest soil. (3) Although the change of different regions in this region was spatially different, keeping the stand cover in the middle and high range of 0.75-0.8 can be conducive to maintaining the balance between the consumption of soil nutrients by the stand and the supplement of nutrient consumption, which can also be conducive to the sustainable recovery and growth of the stand in this region.

Mechanism of catalytic subcritical water oxidation of m-nitroaniline and nitrogen conversion by CuCo2O4 catalyst

Catalytic subcritical water oxidation (CSWO) of M−nitroaniline (M−NA) was investigated in a fused quartz tube reactor (FQTR) to determine the effects of reaction parameters on M−NA mineralization rate, total nitrogen (TN) removal rate and nitrogen-containing products distribution. The results indicated that the TN removal rate was only 26.36 % at 200 °C, 200 % excess oxidant and 15 min residence time, while the TN removal rate reached 67.83 % at 360 °C in SWO of M−NA. Compared with SWO of M−NA, the mineralization rate and TN removal rate of CSWO of M−NA increased by about 10 % and 17.83 %, respectively. The main reason is mainly attributed to CuCo2O4 catalyzing the preferential reaction of ·NO2 with ·NH4, hastening N2 generation. Alternatively, the CuCo2O4 catalyst may induce a direct reaction between the nitro and amino groups on M−NA within the benzene ring to form N2. Moreover, degradation pathways and nitrogen migration mechanism of M−NA under SWO/CSWO conditions were proposed. These crucial findings are expected to guide the development of environmentally sustainable catalytic technologies towards addressing significant water pollution challenges.

Revealing the nitrogen migration mechanism during pyrolysis and steam gasification of biomass: A combined ReaxFF MD and DFT study

The biomass pyrolysis/gasification technology takes advantages of high efficiency and carbon neutrality, which is promising for high value-added products fabrication. However, biomass possesses relatively high amount of N especially for seaweeds and municipal sludge wastes, and the lack of N migration mechanism has resulted in emission of N-containing pollutants and limited its industrial application. In this work, a simulation investigation combining reactive force field molecular dynamic and density functional theory calculations was performed to study the conversion mechanisms of N-containing species in the pyrolysis/gasification of biomass processes. The glutamic acid and cellulose were selected as the model compounds of N-containing and typical compositions of biomass, respectively, and six models containing multiple mass ratios of glutamic acid to cellulose and steam to feedstock were constructed. The results show that the majority of nitrogen in the original model is converted towards NH3-N (62%) during glutamic acid pyrolysis by direct cleavage, hydrogen capture, deamination, and decarboxylation. The presence of cellulose effectively inhibits the decomposition of NH3 at high temperature range, thereby increasing the yield of NH3-N (up to 70%) and restraining the conversion of nitrogen into mesomolecules. The introduction of H2O provides a substantial amount of •H radicals, which facilitates the conversion of nitrogen into NH3-N. During DFT calculations, eight reaction pathways for the formation of N-containing products were proposed on the basis of distribution of pyrolysis products. The glutamic acid cleavage reaction achieves the lowest energy barrier, and the deamination of glutamic acid towards a ternary cyclic ether is found to be the most feasible pathway for the NH3 production. This work can provide theoretical support for nitrogen pollutants mitigation during pyrolysis and steam gasification of biomass processes.

Replacing chemical fertilizers with biogas slurry is an environment friendly strategy to reduce the risk of soil nitrogen leaching: evidence from the HYDRUS model simulation

Excessive chemical fertilizer use leads to soil nitrogen leaching. Biogas slurry, which is rich in nitrogen, has been proposed as an organic substitute for chemical fertilizer via drip irrigation. However, the dynamics of nitrogen migration and potential threats to biogas slurry return remain unclear. Using a two-year field experiment and HYDRUS software, this study modeled soil moisture and nitrogen migration under biogas slurry drip irrigation. The validated model accurately simulates water and nitrogen migration, predicting nitrogen leaching dynamics. Compared to chemical fertilizer treatment, differences in soil hydraulic parameters under biogas slurry drip irrigation impact moisture and nitrate nitrogen distribution. Increasing biogas slurry proportion enhances nitrate nitrogen content in the root layer, promoting plant nitrogen absorption while reducing leaching by 49.66%. Optimizing the irrigation system involves high concentration substitution in early growth and low concentration in late growth, mitigating nitrogen leaching, and enhancing plant root absorption.

Contaminant distribution and migration in roofing rainwater: Implications for sustainable utilization and pollution control

Understanding contaminant distribution and migration characteristics is crucial for the sustainable utilization of roofing rainwater resources, given the impacts of rapid urbanization and climate change. This study undertook the monitoring of 15 typical rainfall events in Xi'an, China, to analyze the distribution and migration of organic matter, nitrogen, and typical emerging contaminants (ECs) during wet deposition (WD), roofing erosion, and roof runoff (RR). The results indicate the concentration levels of organic matter, and nitrogen as follows: COD, 22.69 ± 1.52–132.83 ± 3.43 mg/L; TOC, 3.45 ± 0.22–27.64 ± 1.12 mg/L; TON, 4.99 ± 0.28–12.38 ± 1.55 mg/L; DTN, 4.54 ± 0.10–11.87 ± 0.83 mg/L; PTN, 1.32 ± 0.14–6.57 ± 0.40 mg/L. Additionally, specific emerging contaminants were found at the following concentrations: endocrine disruptors, 19.65–178.85 ng/L; pharmaceuticals, 17.78–67.45 ng/L; bactericides, 4.42–119.45 ng/L; personal care products, 7.79–44.10 ng/L; pesticides, 5.50–34.85 ng/L; flame retardants, 1.80–20.90 ng/L. The nitrogen pollution level surpasses that of road runoff and is predominantly attributed to WD. The detection rate of ECs is notably high, with Bisphenol A (BPA) being detected in up to 100 % of samples. Significant differences in pollutant distribution were identified between the initial and late stages of rainfall (P < 0.05). Weak first flush effects (1 < MFF20 < 2) were observed for pollutants present in the runoff of over 50 % of the rainfall events monitored. Furthermore, by controlling the initial 20 % of WD, an effective decrease in nitrogen flux below the threshold level was observed. Consequently, four modes of contaminant migration pathways and a novel strategy have been developed to guide the pollution control and sustainable utilization of roofing rainwater.

Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water.

Recently, probiotics have been widely applied for the in situ remediation of aquatic water. Numerous studies have proved that probiotics can regulate water quality by improving the microbial community. Nitrogen cycling, induced by microorganisms, is a crucial process for maintaining the balance of the aquatic ecosystem. Nevertheless, the underlying mechanisms by which probiotics enhance water quality in aquatic systems remain poorly understood. To explore the water quality indicators and their correlation with nitrogen cycling-related functional genes, metagenomic analysis of element cycling was performed to identify nitrogen cycling-related functional genes in Coilia nasus aquatic water between the control group (C) and the groups supplemented with probiotics in feed (PF) or water (PW). The results showed that adding probiotics to the aquatic water could reduce the concentrations of ammonia nitrogen (NH4+-N), nitrite (NO2--N), and total nitrogen (TN) in the water. Community structure analysis revealed that the relative abundance of Verrucomicrobiota was increased from 30 d to 120 d (2.61% to 6.35%) in the PW group, while the relative abundance of Cyanobacteria was decreased from 30 d to 120 d (5.66% to 1.77%). We constructed a nitrogen cycling pathway diagram for C. nasus aquaculture ponds. The nitrogen cycle functional analysis showed that adding probiotics to the water could increase the relative abundance of the amoC_B and hao (Nitrification pathways) and the nirS and nosZ (Denitrification pathways). Correlation analysis revealed that NH4+-N was significantly negatively correlated with Limnohabitans, Sediminibacterium, and Algoriphagus, while NO2--N was significantly negatively correlated with Roseomonas and Rubrivivax. Our study demonstrated that adding probiotics to the water can promote nitrogen element conversion and migration, facilitate nitrogen cycling, benefit ecological environment protection, and remove nitrogen-containing compounds in aquaculture systems by altering the relative abundance of nitrogen cycling-related functional genes and microorganisms.

Seasonal nitrate variations, risks, and sources in groundwater under different land use types in a thousand-year-cultivated region, northwestern China

The global public health concern of nitrate (NO3−) contamination in groundwater is particularly pronounced in irrigated agricultural regions. This paper aims to analyze the spatial distribution of groundwater NO3−, assess potential health risks for local residents, and quantitatively identify nitrate sources during different seasons and land use types in the Jinghuiqu Irrigation District, a region in northwestern China with a longstanding agricultural history. The investigation utilizes hydrochemical parameters, dual isotopic data, and the Bayesian stable isotope mixing model (MixSIAR). The findings underscore significant seasonal variations in the average concentrations of NO3−, with values of 87.72 mg/L and 101.87 mg/L during the wet and dry seasons, respectively. Furthermore, distinct fluctuations in nitrate concentration were observed across different land use types, whereby vegetable lands manifested the maximum concentration. Prolonged exposure to elevated nitrate concentrations may pose potential health risks to residents, especially in the dry season when the non-carcinogenic groundwater nitrate risk surges past its wet season counterpart. The MixSIAR analysis revealed that chemical fertilizers accounted for the majority of nitrate pollution in vegetable lands, both during the dry season (49.6%) and wet season (41.2%). In contrast, manure and sewage contributed significantly to NO3−concentrations in residential land during the wet (74.9%) and dry seasons (67.6%). For croplands, soil nitrogen emerged as a dominant source during the wet season (42.2%), while chemical fertilizers prevailed in the dry season (38.7%). In addition to source variations, the nitrate concentration of groundwater is further affected by hydrogeological conditions, with more permeable aquifers tending to display higher nitrate concentrations. Thus, targeted measures were proposed to modify or impede the nitrogen migration pathway, taking into consideration hydrogeological conditions and incorporating domestic sewage, organic fertilizer, and agricultural management practices.

Experimental and ReaxFF MD study on the release characteristics of gaseous nitrogen during pressurized bituminous coal gasification

Coal gasification is the leading technology of clean coal technology such as Integrated Gasification Combined Cycle (IGCC). The release pattern of gaseous nitrogen during coal gasification is crucial for clean coal technology. This paper investigated the gaseous nitrogen emission characteristics of pressurized gasification through experiments and molecular dynamics studies. The experimental results show that the elevated temperatures promoted the release of NO and accelerated the conversion of nitrogen-containing precursors (HCN, NH3). However, high pressure weakened the effect of temperature and resulted in the decreased emissions of HCN, NH3, and NO. Because high pressure facilitated the conversion of fuel nitrogen to nitrogen. The emissions of HCN and NH3 decreased as the CO2 blending ratio increased, while NO emissions increased. The observed difference was primarily attributed to the amounts of free radicals and was also related to gas-phase reactions involving H2O and CO2. Furthermore, as the primary nitrogen-containing structure in bituminous coal, the pyrrole was used in Reactive force field molecular dynamics (ReaxFF MD) to study the release of gas-phase nitrogen during gasification process. The simulation results show that both temperature and pressure promote the migration of pyrrole nitrogen and also provided the main nitrogen migration paths during gasification which can be used to (indirectly) confirm the experimental analysis. The O2/N2/CO2 gasification system supplied additional O and OH radicals to enhance NO emissions, while H2O directly participated in reaction paths such as CN, NCO → NHi or provided H radicals to increase NH3 emissions.

Rainfall Runoff and Nitrogen Loss Characteristics on the Miyun Reservoir Slope

Rainfall intensity and slope gradient are the main drivers of slope surface runoff and nitrogen loss. To explore the distribution of rainfall runoff and nitrogen loss on the Miyun Reservoir slopes, we used artificial indoor simulated rainfall experiments to determine the distribution characteristics and nitrogen migration paths of surface and subsurface runoff under different rainfall intensities and slope gradients. The initial runoff generation time of subsurface runoff lagged that of surface runoff, and the lag time under different rainfall intensity and slope conditions ranges from 3.97 to 12.62 min. Surface runoff rate increased with increasing rainfall intensity and slope gradient; compared with a rainfall intensity of 40 mm/h, at a slope of 15°, average surface runoff rate at 60 and 80 mm/h increased by 2.38 and 3.60 times, respectively. Meanwhile, the subsurface runoff rate trended upwards with increasing rainfall intensity, in the order 5 &gt; 15 &gt; 10°. It initially increased and then decreased with increasing slope gradient, in the order 5 &gt; 10 &gt; 15°. Total nitrogen (TN) loss concentration of surface runoff shows a decrease followed by a stabilization trend; the concentration of TN loss decreases with decreasing rainfall intensity, and the stabilization time becomes earlier and is most obvious in 5° slope conditions. TN loss concentration in subsurface runoff decreased with increasing rainfall intensity, i.e., 40 &gt; 60 &gt; 80 mm/h. The surface runoff rainfall coefficient was mainly affected by rainfall intensity, a correlation between αs and slope gradients S was not obvious, and the fitting effect was poor. The subsurface runoff rainfall coefficient was mainly affected by slope gradient, the R2 of all rainfall intensities was &lt;0.60, and the fitting effect was poor. The main runoff loss pathway from the Miyun Reservoir slopes was surface runoff, which was more than 62.57%. At the same time, nitrogen loss was subsurface runoff, more than 51.14%. The proportion of surface runoff to total runoff increases with the increase of rainfall intensity and slope, with a minimum of 62.57%, and the proportion of nitrogen loss from subsurface runoff also decreases with increasing rainfall intensity but does not change with slope gradient. The order of different runoff modulus types was mixed runoff (surface and subsurface runoff occur simultaneously) &gt; surface runoff &gt; subsurface runoff. The surface and mixed runoff modulus increased significantly with increasing rain intensity under different rain intensities and slope gradients. Overall, rainfall intensity significantly affected slope surface runoff, and slope gradient significantly affected nitrogen loss.

Influence of low-density polyethylene addition on nitrogen transformation during sludge protein pyrolysis

Co-pyrolysis of sludge and waste plastics is a long-term strategic approach for producing valuable fuels. However, sludge is characterized by a high protein content, which serves as a significant source of nitrogenous components in bio-oil. To improve the quality of pyrolysis products, the present study investigated the effect of low-density polyethylene (LDPE) on nitrogen transformation during the pyrolysis of sludge protein (SP). The study findings indicated that LDPE addition reduced the amount of solid residue owing to the interaction between SP and H radicals generated by LDPE pyrolysis. The thermal weight loss difference consistently remained below 0 when the SP-to-LDPE mixing ratio was 7:3, indicating that LDPE promotes the pyrolysis of SP. For the analysis of nitrogen-containing products during pyrolysis, LDPE addition significantly reduced the levels of nitrogen-containing compounds in the bio-oils of glutamate (Glu), histidine (His), and tyrosine (Tyr). Specifically, at 600°C, Glu, His, and Tyr in the bio-oils decreased by 31.4%, 33.3%, and 34.38%, respectively. No significant changes were observed in the nitrogen content within the pyrolysis char of Glu and His. However, all three amino acids exhibited a significant increase in the formation of NOx precursors due to the attack on the nitrogen positions of the heterocyclic nitrogen species by the H radicals generated by LDPE. This study contributes to research on the impact of LDPE on nitrogen migration and conversion during the pyrolysis of SP, laying the foundation for subsequent studies on pollutant removal reactions.

Study on the Contribution of Groundwater Nitrogen Pollution Sources in a Typical Section of the Wei River in China

Aiming to assess the groundwater nitrogen pollution problem in a typical section of the Wei River in China, the contribution of groundwater nitrogen pollution sources in the region was studied. Using Hydrus-1D to implement the simulation process of substituting points for surfaces, we calculated the volume and nitrogen concentration of the water leaching out from the bottom of the encompassing aeration zone. The results of the Hydrus-1D simulation were input as initial values into the nitrogen migration and transformation numerical model constructed using Visual MODFLOW to integrate the simulation calculations between the surface, the aeration zone, and the aquifer system. In addition, the contribution rates of different groundwater nitrogen pollution sources were calculated using the equilibrium formula combined with the groundwater nitrogen test results. The simulation results showed that the groundwater nitrogen in the southern part of the Wei River comes from two main sources: vertical infiltration and river recharge. Specifically, ammonia nitrogen vertical infiltration and river recharge contribute 95.82% and 4.18%, respectively; nitrite nitrogen vertical infiltration and river recharge contribute 92.41% and 7.59%; and nitrate nitrogen vertical infiltration and river recharge contribute 94.26% and 5.74%. According to the simulation results, an increase in the intensity of surface water pollution control is required in the study area. It is also necessary to control the use of nitrogen fertiliser on farmland in the study area and improve the utilisation rate of nitrogen fertiliser to reduce the nitrogen pollution loads from these sources.

Nitrogen Loss and Migration in Rice Fields under Different Water and Fertilizer Modes.

Irrigating aquaculture wastewater in appropriate irrigation and drainage modes in paddy fields could reduce water and fertilizer loss. However, the precise mechanisms involved in the degradation and movement of nitrogen in various water and fertilizer modes are still not fully understood. This study involves conducting a controlled experiment using barrels to examine the effects of various water quality, irrigation and drainage methods, and fertilization levels. The aim is to analyze the patterns of nitrogen degradation, loss, migration, and absorption in surface water, underground drainage, and soil leakage at different depths. The results showed the following: (1) The paddy field has a significant purification effect on aquaculture wastewater after one day of irrigation, reached at 78.55-96.06%. (2) Aquaculture wastewater irrigation increased nitrogen concentration in the plough layer, which helps rice roots absorb nitrogen and boosts plant TN. (3) In special dry years, underground seepage is the predominant method of nitrogen loss, and underground drainage nitrogen concentration peaks 2-6 days after fertilization. (4) Under aquaculture wastewater irrigation, the TN loss load of II decreased by 27.65-42.45% than FSI. Compared with IA-80, the TN degradation rate of IA in surface water increased by 18.51%, TN loss load decreased by 5.48%, TN absorption rate significantly increased by 14.61%, and yield increased by 31.14% significantly. IA is recommended in special dry years, which can improve the TN absorption rate and ensure high yield while significantly reducing the loss load of nitrogen. The findings can provide a basis for the purification of aquaculture wastewater through paddy field ecosystems in response to fertilizer supply levels.

Unveiling the dynamic of nitrogen through migration and transformation patterns in the groundwater level fluctuation zone of a different hyporheic zone sediment

This study investigates the impact of water levels and soil texture on the migration and transformation of nitrate (NO3−-N) and ammonium (NH4+-N) within a soil column. The concentrations of NO3−-N gradually decreased from an initial concentration of 34.19 ± 0.86 mg/L to 14.33 ± 0.77 mg/L on day 70, exhibiting fluctuations and migration influenced by water levels and soil texture. Higher water levels were associated with decreased NO3−-N concentrations, while lower water levels resulted in increased concentrations. The retention and absorption capacity for NO3−-N were highest in fine sand soil, followed by medium sand and coarse sand, highlighting the significance of soil texture in nitrate movement and retention. The analysis of variance (ANOVA) confirmed statistically significant variations in pH, dissolve oxygen and oxidation–reduction potential across the soil columns (p < 0.05). Fluctuating water levels influenced the migration and transformation of NO3−-N, with distinct patterns observed in different soil textures. Water level fluctuations also impacted the migration and transformation of NH4+-N, with higher water levels associated with increased concentrations and lower water levels resulting in decreased concentrations. Among the soil types considered, medium sand exhibited the highest absorption capacity for NH4+-N. These findings underscore the significant roles of water levels, soil texture, and soil type in the migration, transformation, and absorption of nitrogen compounds within soil columns. The results contribute to a better understanding of nitrogen dynamics under varying water levels and environmental conditions, providing valuable insights into the patterns of nitrogen migration and transformation in small-scale soil column experiments.

Study on Nitrogen Migration during Co-Pyrolysis of Melamine-Impregnated Paper Waste and Camellia Oleifera Shell

Melamine-impregnated paper waste (MIPW) is an unavoidable byproduct of the melamine-impregnated paper production process, and it must be urgently disposed. In this paper, due to its high N content, MIPW was used as a N source to co-pyrolyze with camellia oleifera shell (COS). By changing the proportion of MIPW in the raw materials, the migration path of the N was studied during the co-pyrolysis process. X-ray photoelectron spectroscopy (XPS), elemental analysis (EA), total nitrogen analysis (TN) and ultraviolet spectrophotometry were used to determine the content and the types of N-containing components in raw materials and products. The results showed that during the co-pyrolysis process, the N in MIPW and COS would be converted into different types of N-containing components, and the proportion of MIPW in raw materials directly impacts the product distribution and the N migration path. With the increase in the proportion of MIPW from 10 to 50 wt.% (daf, mass ratio), the proportion of N in raw materials that migrated into the solid phase decreased from 27.58 to 16.31 wt.%, while that which migrated to the gas and liquid phases increased from 24.92 and 47.50 wt.% to 25.97 and 57.72 wt.%, respectively. The proportions of N in the raw materials that changed into pyridinic-N, pyrrolic-N and graphitic-N in the solid phase and N2-N in the gas phase were decreased. The proportions of NH3-N and HCN-N in the gas phase and NH3-N and organic-N in the liquid phase were increased. Moreover, synergies were observed during the co-pyrolysis process, which affected the N migration path in the raw material. This study will provide policy directions and theoretical support for the comprehensive utilization of MIPW and COS.

Relationship between Nitrogen Dynamics and Key Microbial Nitrogen-Cycling Genes in an Intensive Freshwater Aquaculture Pond.

Intensive aquaculture in high-density hybrid snakehead [Channa maculata (♀) × Channa argus (♂)] fishponds can lead to toxic conditions for fish. This study investigated nitrogen migration and transformation in these fishponds during different cultivation periods. Using qPCR technology, we analyzed the abundance variation of nitrogen-cycling microorganisms in water and sediment to reveal the nitrogen metabolism characteristics of hybrid snakehead fishponds. The results showed that fish biomass significantly impacts suspended particulate matter (SPM) flux. At the sediment-water interface, inorganic nitrogen fluxes showed predominant NO3--N absorption by sediments and NH4+-N and NO2--N release, especially in later cultivation stages. Sediments were rich in nirS and AMX 16S rRNA genes (ranging from 4.04 × 109 to 1.01 × 1010 and 1.19 × 108 to 2.62 × 108 copies/g, respectively) with nirS-type denitrifiers potentially dominating the denitrification process. Ammonia-oxidizing bacteria (AOB) were found to dominate the ammonia oxidation process over ammonia-oxidizing archaea (AOA) in both water and sediment. Redundancy analysis revealed a positive correlation between SPM flux, Chlorophyll a (Chl-a), and denitrification genes in the water, and between nitrogen-cycling genes and NH4+/NO2- fluxes at the interface. These findings provide a scientific basis for nitrogen control in hybrid snakehead fishponds.

Plant-microorganism-soil interaction under long-term low-dose ionizing radiation.

As the environmental nuclear radiation pollution caused by nuclear-contaminated water discharge and other factors intensifies, more plant-microorganism-soil systems will be under long-term low-dose ionizing radiation (LLR). However, the regulatory mechanisms of the plant-microorganism-soil system under LLR are still unclear. In this study, we study a system that has been stably exposed to low-dose ionizing radiation for 10 years and investigate the response of the plant-microorganism-soil system to LLR based on the decay of the absorbed dose rate with distance. The results show that LLR affects the carbon and nitrogen migration process between plant-microorganism-soil through the "symbiotic microbial effect." The increase in the intensity of ionizing radiation led to a significant increase in the relative abundance of symbiotic fungi, such as Ectomycorrhizal fungi and Rhizobiales, which is accompanied by a significant increase in soil lignin peroxidase (LiP) activity, the C/N ratio, and C%. Meanwhile, enhanced radiation intensity causes adaptive changes in the plant functional traits. This study demonstrates that the "symbiotic microbial effect" of plant-microorganism-soil systems is an important process in terrestrial ecosystems in response to LLR.

Experimental Study on Compaction Deformation and Gas Permeability Properties for Crushed Limestone.

The mining goaf is enriched in coalbed methane (CBM) resources, and it is imperative to realize its efficient extraction. The gas permeability properties of the crushed coal and rock in the caved zone of mining goaf are the basis for the study of its internal CBM migration and enrichment law. In this study, the compaction deformation and gas permeability properties of crushed limestone with different particle sizes were revealed. The results show that (1) the deformation resistance capacity of the crushed limestone increased with increasing stress. The decreasing trend of porosity of samples with different particle sizes in the early and later compression periods is significantly different. Particle RR of the lower layer is smaller than that of the other layers. (2) The permeability of the sample decreases with decreasing porosity and nitrogen pressure, and it is between 10-12 and 10-10 m2. Nitrogen migration within the crushed limestone requires the pseudo-threshold pressure gradient, which ranges from 64.86 to 311.42 Pa/m. (3) The average permeability growth amplitude of the sample shows a logarithmic decreasing trend with the decrease of porosity. The average permeability growth amplitude of the 5-10 mm sample at the same porosity was 15.9-22.3 times that of the 0.315-0.63 mm sample. (4) The permeability of crushed limestone on both sides of the lower layer in the caved zone is much larger than that of other locations. The results are of great practical significance for accurately predicting the CBM enrichment area of mining goaf and then selecting the final position of the extraction drilling hole.

Metals and biogenic substances’ migration ability in the «bottom sediments – water» system under natural and experimental conditions

&#x0D; &#x0D; &#x0D; The paper considers the results of long-term studies on some chemical elements’ migration (Al, Fe, Ti, Mn, Cu, Zn, Pb, N, P, Si) in the «bottom sediments – water» system of surface water bodies under the effect of different aquatic environment factors. The greatest effect is made by water bodies’ oxygen regime, pH, and presence of dissolved organic substances, in particular humic substances. The migration of manganese, iron, inorganic nitrogen and phosphorus from bottom sediments is controlled mostly by oxygen regime. Migration of these chemical elements significantly increases, when there is oxygen deficiency and anaerobic conditions are formed in the bottom water layer. It has been observed in both natural and experimental conditions. Man- ganese concentration increases in bottom water in 25–50 times, iron – in 1.3–13, inorganic nitrogen – in 5.3–19.3, and inorganic phosphorus – in 2.8–23 times. The dissolved oxygen concentration hardly has any effect on the migration of aluminium, titanium, copper, zinc, lead, and silicon from bottom sediments into the water. The chemical elements’ migration is significantly affected by a decrease in pH of water contacting with bottom sediments and the presence of humic substances in it. High humic substances concentrations promote a reduction in water pH and oxygen content, which is consumed for their oxidation. A case study of several water bodies illustrates the cumulative effect of water pH lowering, anaerobic conditions at the solid and liquid phases’ interface, as well as complexation with humic substances on chemical elements’ migration from bottom sediments. Experimental modeling has shown that the metal migration from bottom sediments occurs both due to their labile fractions and complex compounds with dissolved organic matter, especially with humic substances of low molecular weight (≤2.0 kDa). The share of the metal labile fraction gets higher, when water pH decreases. Under recent climate change, the probability of water’s secondary pollution with different chemicals increases significantly in summer. This is mainly caused by oxygen deficiency, water pH lowering, and reducing conditions at the «bottom sediments – water» interface with hydrogen sulphide being formed. This is especially true for highly eutrophic water bodies subject to human impact. The aquatic environment toxicity can get considerably higher due to the migration of chemicals with strong toxic properties from bottom sediments, as well as labile metal fractions, marked by higher bioavailability for hydrobionts.&#x0D; &#x0D; &#x0D;

Research on Underground Drip Irrigation and Soil Redistribution - Take Heshan District of Yiyang City as an Example

Abstract: The drip flow and the buried depth of drip irrigation zone are not only important parameters of subsurface irrigation design and operation management, but also important factors affecting the migration and redistribution of soil water and fertilizer. Crop growth is closely related to soil water and nitrogen leaching, water and fertilizer transport patterns and redistribution characteristics. It is of great significance to study the migration and redistribution of soil water and fertilizer under subsurface drip irrigation for improving the utilization efficiency of water and fertilizer and improving the system of subsurface drip irrigation. In this paper, the migration mode and redistribution characteristics of water and nitrogen in subsurface drip irrigation were studied and analyzed by means of laboratory soil box experiment, considering the two factors of drip flow and the buried depth of drip irrigation belt. The main research conclusions are as follows: Ⅰ. Different drip flows had effects on soil moisture front, soil moisture content and soil water redistribution. If other conditions remain unchanged, increasing the drip flow can increase the moist body area of soil and increase the moisture content of soil. The distance between vertical and horizontal directions increased with the large drip flow, and the soil water content decreased with the increase of distance. Large drip flow can promote the redistribution of soil water after drip irrigation. Ⅱ. Different drip depth had effects on soil moisture content and soil water redistribution. When the drip depth was 0cm, the surface shape of the wet body was a "horizontal" semi-ellipse after drip irrigation. When the drip depth was 10cm and 20cm, the surface of the wet body was an "upright" semi-ellipse after drip irrigation. The larger the buried depth of the drip can make the distribution of soil moisture more uniform, and the larger the buried depth of the drip can promote the migration of water in the soil. Ⅲ. Different drip discharge had an effect on soil nitrogen redistribution, and large drip discharge promoted soil nitrogen redistribution after drip irrigation. The Nitrate Nitrogen (NO3--N) content in the moist body is lower than that near the edge of the wetting front. The content of Ammonium Nitrogen (NH4+-N) near the edge of the wetting peak is higher than that inside the wetting body. Ⅳ. The redistribution of soil nitrogen was affected by different buried depth of drip head, and the greater buried depth of drip head could promote the redistribution of soil nitrogen. In terms of vertical and horizontal migration of ammonium nitrogen and nitrate nitrogen, on the day after drip irrigation, the cumulative effect of Nitrate Nitrogen (NO3--N) buried at a depth of 20cm was more significant near the wetting front edge at a larger vertical downward distance, and the cumulative effect of Nitrate Nitrogen (NO3--N) buried at a depth of 20cm was more significant inside the wetting body at a larger vertical downward distance. At the same time, the drip buried depth of 20cm can spread the cumulative effect inside the wet body and near the edge of the wet front to a longer horizontal distance.