Middle Channel Research Articles

Riverbed Changes of the Uppermost Atchafalaya River, USA—A Case Study of Channel Dynamics in Large Man-Controlled Alluvial River Confluences

River confluences are important nodes for downstream sediment transport and geomorphological development. Previous studies have established the knowledge that under natural conditions, river confluence zones experience channel scour followed with middle channel bar development. Less care is however given to the intensity of a confluence scour zone under man-controlled conditions, such as discharge regulation and levee confinement. In general, our knowledge about long-term bed evolution downstream of large alluvial river confluences is limited. Here we conducted a study focused on the 69-km uppermost channel of the Atchafalaya River, the largest distributary of the Mississippi River, to test the hypothesis that the channel downstream of two large tributaries sustains longer-term, extensive bed scouring owing to increased discharge in the main channel and, therefore, mid-channel bars in such a confluence zone cannot be built under confined channel conditions. The Atchafalaya River carries the total flow from the Red River and approximately 25% of the Mississippi River flow, traveling southwards 230 km before emptying into the Gulf of Mexico. We utilized long-term records on water surface elevation and discharge during 1935–2016, as well as channel bathymetry survey data during 1998–2006 to determine changes in hydraulic head and morphologic deformation in the confluence zone. The results from this study show that the combined flow from the Red River and Mississippi River into the Atchafalaya River steadily increased to approximately 5848 cubic meters per second (m3 s−1) in the recent decades, and the channel bed of the uppermost Atchafalaya River experienced considerable erosion since the 1930s. At a specific discharge of 8000 ± 100 m3 s−1, the river stage decreased by 5.8, 5.6, and 4.9 m from 1935 to 2016 at (from upstream to downstream) Simmesport, Melville, and Krotz Springs gauging stations, respectively. The average bed elevation reduced by 1.9 m from 1998 to 2006, although its thalweg increased by 0.3 m. Based on the channel bed assessment, a total volume of 6.6 × 107 m3 sediment was eroded from the uppermost 69 km of the Atchafalaya over the 8 years. The findings suggest that confluence zones of large alluvial rivers under controlled flow and confined levee conditions can experience extensive, long-lasting channel erosion. This can be especially progressive if the channel below a confluence is confined by levees, which can increase drag forces and prevent middle channel deposition. Further studies are needed to determine if the eroded sediment from the uppermost Atchafalaya is carried out to the river mouth or is deposited in the lower Atchafalaya. Such knowledge will have both scientific and practical relevance in river engineering and management.

Microengineered hiPSC-Derived 3D Amnion Tissue Model to Probe Amniotic Inflammatory Responses under Bacterial Exposure.

Intra-amniotic infection is a common cause of preterm birth that can lead to adverse neonatal outcomes. Despite the basic and clinical significance, the study in normal and diseased human amnion is highly challenging due to the limited use of human primary tissues and the distinct divergence between animal models and human. Here, we established a microengineered hiPSC-derived amnion tissue model on a chip to investigate the inflammatory responses of amnion tissues to bacterial exposure. The microdevice consisted of two parallel channels with a middle matrix channel, creating a permissive microenvironment for amnion differentiation. Dissociated hiPSCs efficiently self-organized into cell cavity and finally differentiated into a polarized squamous amniotic epithelium on the chip under perfused 3D culture. When exposed to E. coli, amnion tissue exhibited significant functional impairments compared to the control, including induced cell apoptosis, disrupted cell junction integrity, and increased inflammatory factor secretion, recapitulating a series of characteristic clinical signs of intra-amniotic infection at an early stage. Together, this amnion-on-a-chip model provides a promising platform to investigate intrauterine inflammation in early gestation, indicating its potential applications in human embryology and reproductive medicine.

On the heat budget and water mass exchange in the Andaman Sea

The characteristics of the T/S structures, water mass exchange and deep circulation in the Andaman Sea are investigated based on the simulation from a high-resolution general circulation model (MITgcm). The results show that, below 1 000 m, the water mass is saltier, warmer and more homogeneous in the Andaman Sea than that in the Bay of Bengal, attributing to the strong vertical mixing at the depth of ∼1 800 m. The water mass exchange between the Andaman Sea and the Bay of Bengal goes through three major channels, which manifests itself as follows: the northern channel (Preparis Channel) is the main passage of water mass transport from the Bay of Bengal to the Andaman Sea, whereas the Middle Channel (the south of Andaman Islands and the north of Nicobar Islands) has an opposite transport; the southern channel (Great Channel) features with a four-layer water exchange which results in the least net transport among the three channels; all the transports through the three channels have an intra-annual variation with a period of half a year. At 1 000-m depth, the entire Andaman Sea is occupied by a cyclonic circulation in January and July while by an anticyclonic one in April and October. The semiannual cycle found in both the deep circulation and water mass exchange is likely associated with the downwelling eastward-propagating Kelvin waves induced by the semiannual westerly component in the equatorial Indian Ocean during intermonsoon seasons.

A Middle Crustal Channel of Radial Anisotropy Beneath the Northeastern Basin and Range

AbstractA challenge in interpreting the origins of seismic anisotropy in deformed continental crust is that composition and rheology vary with depth. We investigated anisotropy in the northeastern Basin and Range where prior studies found prevalent depth‐averaged positive radial anisotropy (VSH > VSV). This study focuses on depth‐dependence of anisotropy and potentially distinct structures beneath three metamorphic core complexes (MCCs). Rayleigh and Love wave dispersion were measured using ambient noise interferometry, and Bayesian Markov chain Monte Carlo inversions for VS structure were tested with several (an)isotropic parameterizations. Acceptable data fits with minimal introduction of anisotropy are achieved by models with anisotropy concentrated in the middle crust. The peak magnitude of anisotropy from the mean of the posterior distributions ranges from 3.5–5% and is concentrated at 8–20 km depth. Synthetic tests with one uniform layer of anisotropy best reproduce the regional mean results with 9% anisotropy at 6–22 km depth. Both magnitudes are plausible based on exhumed middle crustal rocks. The three MCCs exhibit ~5% higher isotropic upper crustal VS, likely due to their anomalous levels of exhumation, but no distinctive (an)isotropic structures at deeper depths. Regionally pervasive middle crustal positive radial anisotropy is interpreted as a result of subhorizontal foliation of mica‐bearing rocks deformed near the top of the ductile deformation regime. Decreasing mica content with depth and more broadly distributed deformation at lower stress levels may explain diminished lower crustal anisotropy. Absence of distinctive deep crustal VS beneath the MCCs suggests overprinting by ductile deformation since the middle Miocene.

MO068A KIDNEY-ON-THE-CHIP APPROACH USING PRIMARY HUMAN TUBULAR CELLS IN A 3D CO-CULTURE SYSTEM

Abstract Background and Aims Conventional 2D mono-culture in vitro models using immortalized cell lines are still widely used in experimental nephrology, although their value is limited by poor translatability and predictive value for the in vivo or even human situation. The implementation of more sophisticated in vitro assays as routine cell culture systems is often limited by complex protocols and long lasting procedures. We aimed to establish and validate a relatively easy-to-use but yet (patho-) physiologically relevant cell culture assay that mimics key aspects of the in vivo situation of renal tubules, including a leak-thight epithelium with a luminal and baso-lateral side, interstitial matrix, a peri-tubular capillary and circulating blood cells inside its lumen. Method We utilized the 3-lane OrganoPlate® system (Mimetas, Leiden, Netherlands) as a scaffold. After infusing a collagen I matrix in the middle channel (C2), primary human renal progenitor cells are seeded into the upper channel (C1), adhering to the C2-matrix. The plate is put on a perfusion rocker (Mimetas), that facilitates continuous gravity-triggered bi-directional perfusion of C1. Within 48h the cells form a leak-tight tubular structure with a continuous lumen. Next, human endothelial cells are seeded into the bottom channel (C3), which adhered to the opposite site of the C2-matrix and – upon continuous perfusion – formed a vessel-like structure with a continuous lumen, as well. Finally, primary human white blood cells were isolated and seeded into C3 (see figure). Results Establishing the whole tubule-on-the-chip as described above takes on average three days. We investigated its operational life span by monitoring the barrier integrity of the tubular structure in C1 using a fluorescence-labeled dextran (150 kDa). Over a course of 5 days the tubular integrity did not decline, suggesting that the co-culture system remains stable and functional for at least 5 days. In accordance with other studies, the primary human tubular cells constituting the 3D tubule-on-the-chip expressed higher levels of functionally relevant proteins, e..g the tight-junction protein ZO-1 and the sodium-potassium-pump Na-K-ATPase, compared to standard 2D settings without perfusion. This emphasizes, that even primary cells show a physiologically reduced phenotype in standard 2D settings, which possibly impedes the identification and representative quantification of physiologically and hence also patho-physiologically relevant mechanisms in vitro. To study the interaction of cells in the tubule-on-the-chip, we investigated the recruitment of immune cells from C3 (vessel) across C2 (interstitium) to C1 (renal tubule), which - in vivo - represents a detrimental mechanism of action in intrarenal forms of AKI. Under baseline conditions the immune cells inserted into C3 did not leave their compartment. Upon damaging the tubular cells in C1 with extracellular histones, neutrophils and monocytes left C3 (extravasation), migrated through C2 and could be found in close contact with epithelial cells of C1. This serves as a proof of principle, that the tubule-on-the-chip is applicable to study complex cell-cell and cell-substrate interactions, such as chemokine-mediate immune cell homing. Measuring lactate dehydrogenase release for a number of known nephrotoxic agents revealed, that tubular cells forming a 3D-structure while kept under perfusion show significantly different responses to the same dose compared to standard 2D conditions, suggesting that dose-response studies using target cells out of their tissues context can be misleading when extrapolating results from in vitro to in vivo. Conclusion The results of this study suggest, that sophisticated 3D co-culture models of a renal tubule including an interstitial compartment, a peri-tubluar capillary and circulating immune cells are feasible and potentially suited to allow for in depth mechanistic studies in vitro.

If you build it and they come, will they stay? Maturation of constructed fish spawning reefs in the St. Clair-Detroit River System

Constructed rock reefs have been used to remediate spawning habitat for Lake Sturgeon (Acipenser fulvescens) and other lithophilic spawning fishes in the St. Clair-Detroit River System, North America. Early projects used a cross-channel design and species-specific metrics (e.g., proximity to historical spawning locations) to guide reef placement. However, the Middle Channel Reefs and portions of other early projects were compromised by fine sediment accumulation. Therefore, geomorphological criteria were considered in siting reefs constructed after 2013 to avoid sediment sources and improve the likelihood of successful reef function. To evaluate the effectiveness of the revised placement process, we quantified physical maturation of constructed reefs using annual side-scan and down-looking sonar surveys beginning in 2014 and underwater video surveys beginning in 2015. Reef areas and hardness were measured from sonar surveys and underwater video was used to quantify surficial sediment composition. Size and hardness of reefs developed using geomorphological criteria decreased with time, but at rates slower than what was observed at the Middle Channel Reefs. Sediment composition of the reefs remained similar through 2017 and prevalence of reef rock was high, except at Hart's Light Reef, where dreissenid mussel shells composed 32% of the surficial substrate by age three. However, accumulation of fine sediments was documented at all reefs in 2018. Despite using geomorphic criteria to identify areas most suitable for reef construction, reef sediment composition has changed, and future reef restoration projects could benefit by incorporating methods for maintenance, in addition to using geomorphic criteria, to identify restoration sites.

Prenatal caffeine exposure induces down-regulation of the protein kinase A/ryanodine receptor/large-conductance Ca2+-activated K+ pathway in the cerebral arteries of old offspring rats

The current study investigated the long-term effects of prenatal caffeine (Caf) exposure on cerebral vessels of old offspring rats. Pregnant rats were treated with Caf (20 mg/kg, twice daily) or 0.9% normal saline during gestational days 3.5-19.5, and offspring were tested at 24 months old. Vascular functions of middle cerebral arteries and ion channel activities in smooth muscle cells were examined using myograph system and patch-clamp. Prenatal Caf exposure decreased isoprenaline (β-adrenergic agonist)-induced dilatation of the middle cerebral artery in the offspring. Treatment with protein kinase A (PKA) inhibitor reduced isoprenaline-mediated vasodilatation to a greater extent in the control. Forskolin-mediated vasodilatation and membrane hyperpolarization were reduced in the Caf group. Large-conductance Ca-activated K (BKCa) channel inhibitor iberiotoxin significantly attenuated forskolin-induced vasodilatation and reduced depolarization in the control, not in the Caf group. The PKA agonist-activated cell-attached single BKCa currents to a greater extent in the control. The mRNA and protein expression levels of PKA-Cα were decreased. The sensitivity of ryanodine receptors to the PKA agonist was blunted in the Caf group, whereas the mRNA expression of ryanodine receptor 2 subunit was reduced. Voltage/Ca sensitivity of BKCa was decreased accompanied by reduced mRNA and protein expression of BKCa-β1 subunits in the Caf group. PKA agonist-stimulated inside-out BKCa currents were weaker in the Caf group. Prenatal exposure to Caf-affected isoprenaline/forskolin-mediated vascular functions in aged cerebral arteries, related to dysfunction of the PKA/ryanodine receptors/BKCa signaling pathway.

Application of seismic attribute analysis in fluvial seismic geomorphology

Seismic attributes can be important predictors, either qualitative or quantitative, of reservoir geometries when they are correctly used in reservoir characterization studies. This paper discusses seismic attribute analyses and their usefulness in seismic geomorphology study of Moragot field of Pattani Basin, Gulf of Thailand. Early to Middle Miocene fluvial channel and overbank sands are the reservoirs in Pattani Basin. Due to their limited horizontal and vertical distribution, it is not always possible to predict the geometry and distribution of these sands based on the conventional seismic interpretation. This study utilized various seismic attributes, e.g., RMS amplitude analysis, spectral decomposition, semblance and dip-steered similarity, RGB blending to image the geometry and the spatial distribution of sand bodies in horizon and stratal slices at different stratigraphic intervals. Attribute analyses reveal, at shallow stratigraphic levels, RMS and semblance can successfully identify channel-shaped sand bodies and mud-filled channels associated with channel belts. On the other hand in deeper stratigraphic intervals, sand distribution can be imaged more effectively by using spectral decomposition and dip-steered similarity volumes. High-frequency spectral decomposition slices can image thin sands, and low-frequency slices can image thick sands quite effectively in deeper intervals. RGB blending of different frequency slices is particularly useful in delineating channel systems of various dimensions at deeper intervals. These images show the distribution of sands and mud-filled channels at various stratigraphic levels. The width of channel belts varies from 200 m to 3 km. These channel belts are N–S or NW–SE oriented. From the channel pattern and their dimensions, depositional environments can be predicted. Mud-filled channels identified in the horizon slices will act as a connectivity barrier between sand bodies at either side of the channel. They can also act as lateral and up-dip seal to form stratigraphic traps. The seismic attribute analyses clearly show the geometry and spatial distribution of sand bodies. Hence, this method for predicting sand body geometry might help in field development planning as well as in reducing exploration risk.

Model-based analysis of geometrical effects in microfluidic fuel cell with flow-through porous electrodes

Porous electrodes in microfluidic fuel cell (MFC) operate with nonuniform reaction rate. It is intriguing to improve the utilization degree of the porous electrodes. In this work, a three-dimensional computational model is developed for MFC with flow-through porous electrodes. Characteristics of the reaction rate distributions under different electrode geometries are examined. The results show that reaction rate varies noticeably along the electrode width direction, but minimally along the electrode length direction. High reaction rate region locates in the vicinity of the interface between the porous electrode and the middle channel. A relatively high aspect ratio, defined as the ratio of the electrode length to width, is beneficial to improve the utilization degree of the porous electrodes. Yet, concentration losses increase due to the decreased fluid velocity. Considering the cell performance, optimal electrode aspect ratios are derived for the anode and cathode, respectively.

Acoustic focusing of microplastics in microchannels: A promising continuous collection approach

Microplastic (MP) pollution is a research topic of the highest priority. Currently, MPs are collected predominantly by sieving and filtration, which is the rate-determining process in contemporary MP research. This study experimentally and theoretically verified the applicability of acoustic focusing for the collection of MPs. As model MPs, polystyrene microparticles and small fibers of Nylon 6 and polyethylene terephthalate were examined. To generate a standing acoustic wave, piezo elements were attached to a microfluidic device, in which the microchannel is trifurcated. A suspension of the microparticles was introduced into the microchannel and focused on its center using an acoustophoretic force, which allowed collecting almost all microparticles from the middle channel of the trifurcated branches. A theoretical examination based on the focusing time of the microparticles indicated that the device should be able to collect MPs of approximately 5 μm in diameter. Similar to the microparticles, the majority of both types of fibers were collected from the middle channel. However, it should be noted that some fibers were attached to the wall of the microchannel and remained therein due to gravitational sinking. Both the experimental results and the theoretical estimations suggest promising potential for acoustic focusing as a means to remove MPs from aqueous suspensions such as the effluent from laundry machines.

Evaluation of Red-Peak Algorithms for Chlorophyll Measurement in the Pearl River Estuary

An algorithm of the red-peak envelop area (PEA) near 700 nm was evaluated using in situ data during nine cruises in the Pearl River estuary and compared with other algorithms using the reflectance peak (RP) near 700 nm, including the fluorescence line height (FLH), maximum chlorophyll index (MCI), and MCI2 algorithms. Of all algorithms, the PEA algorithm presented the most accurate performance [ $R^{2}= 0.74$ , root-mean-square error RMSE = 0.12] and provided a more rational spatial distribution of phytoplankton blooms when both Sentinel 3 Ocean and Land Color Instrument (OLCI) and Hyperion data were used because the PEA integrates information from both the moving peak and the asymmetric curve on each side of the peak due to the high correlation relationship ( $R^{2}= 0.7$ ) of chlorophyll and the ratio of the peak area between the left and right halves. Moreover, compared with other algorithms, the PEA algorithm developed using the Hyperion (higher spectral resolution) and OLCI band settings presented similar retrieval accuracies. These results demonstrated that the PEA algorithm is less dependent on the band settings, and the spectral band settings of OLCI from 650 to 750 nm are reasonable and can be used to detect phytoplankton blooms if the PEA algorithm is applied. The OLCI PEA algorithm was applied to determine the variations in phytoplankton blooms under the influences of strong precipitation events. The most obvious increases in chlorophyll concentration (from 20 to 30 mg $\text{m}^{-3}$ ) were observed in the middle river channel upstream of the Pearl River estuary after strong precipitation events.

Enhanced total nitrogen removal performance in a full scale Orbal oxidation ditch by a novel step aeration mode

Two aeration modes, namely point aeration and step aeration, were proposed and implemented in a full scale Orbal oxidation ditch, and nitrogen removal performance was studied. The results showed that nitrogen removal performance under point aeration mode depended on oxygen supply control. Highest total nitrogen (TN) removal efficiency of 73.2% was achieved when oxygen input in the outer, middle and inner channel accounted for 50, 25 and 25% of total oxygen supply, respectively. With the same oxygen supply, both aeration modes demonstrated complete nitrification with over 97% ammonia nitrogen removal efficiencies. However, TN removal efficiency was 78.8% under step aeration mode, which was higher than that under point aeration. The pyrosequencing results indicated that microbial community composition was affected by aeration modes and step aeration mode was beneficial to the enrichment of denitrifiers. The greater diversity and relative abundance of denitrifiers enhanced TN removal under step aeration mode.

Enhancement in Desalination Performance of Battery Electrodes via Improved Mass Transport Using a Multichannel Flow System.

Desalination technologies have heavily been investigated to utilize the abundant salt water on Earth due to the global freshwater shortage. During recent years, the desalination battery (DB) has attracted attention for its low-cost, eco-friendly, and energy-efficient characteristics. However, the current DB system is subject to inevitable performance degradation because of the mass-transfer limitation at the electrode-electrolyte interface, particularly when the system is used to treat brackish water. Here, we present a novel strategy to overcome the intrinsic mass-transfer limitation of DB in brackish water using an effective cell design based on a multichannel flow system. Compared to the conventional DB that consists of one feed channel, the multichannel desalination battery (MC-DB) is configured using two side channels introducing a highly concentrated solution to the electrodes and one middle feed channel for water desalination. The MC-DB showed a desalination capacity of 52.9 mg g-1 and a maximum salt removal rate of 0.0576 mg g-1 s-1 (production rate of 42.3 g m-2 h-1) when a salinity gradient between the feed streams in the middle (10 mM NaCl) and side (1000 mM NaCl) channels was present, which were 3-fold higher than those in the case with no salinity gradient. In addition, the high concentration solution in the side channel significantly enhanced the rate capability of MC-DB, allowing the system to operate under a high current density of 40 A m-2 with a desalination capacity of 34.1 mg g-1. Considering the effect of electrolyte concentration on the battery electrode performance through electrochemical characterization, the highly saline medium at the side channel in the MC-DB creates an optimal environment for the battery electrode to fully capitalize the high desalination capacity, salt removal rate, and capacity retention of the battery electrodes.

Investigation of heat transfer and fluid flow behaviors of CuO/(60:40)% ethylene glycol and water nanofluid through a serpentine milichannel heat exchanger

PurposeThis paper aims to investigate the three-dimensional (3D) numerical simulations, based on the Navier–Stokes equations and the energy equation. Forced convection of a mixture of (60:40) percent ethylene glycol and water, was used as the base fluid and CuO nanoparticles, through a serpentine minichannel.Design/methodology/approachIn this simulation, a serpentine mini-channel heat exchanger was simulated. The fluid studied in this simulation was composed of a mixture of (60:40) per cent ethylene glycol and water, was used as the base fluid and CuO nanoparticles. Four slabs and three serpentines were used in this study. The serpentine section is connected to the slab. Three equidistant circular channels (1 mm in diameter) were implemented inside the slab.FindingsResults show that nanoparticles increase the fluid pressure drop and by changing volume fraction of nanoparticles from 0 to 1 per cent, the pressure drop of nanofluids increases between 42and 47 per cent, for Reynolds numbers from 100 to 500. The existence of serpentine bend in the minichannel heat exchanger causes the heat transfer rate to increase. Increase the volume fraction of nanoparticles reduces the fluid temperature at the outlet of the heat exchanger. The numerical results show that in Re = 500, at the beginning of the last slab in middle channel by changing volume fraction of nanoparticles from 0 to 2 per cent, local Nusselt number 57.40 per cent increase. The existence of the serpentine bend causes the heat transfer rate to increase.Originality/valueForced convection of a mixture of (60:40) per cent ethylene glycol and water by using of 3D numerical simulations, based on the Navier–Stokes equations.

Multi-physics Simulation of Heat Transfer in Pulse Electrochemical Machining (PECM) Process

A multi-physics model is presented for numerical simulation of temperature evolution during quasi steady state (QSS) in pulse electrochemical machining (PECM) process. A fluid model consisting of wall-model and Reynolds-averaged Navier-Stokes equations is used for solving low-Re electrolyte flow near the wall and turbulent flow of electrolyte in the middle channel. A method that the boundary conditions and bulk sources are averaged in time is introduced to make the time-steps not dictated by the time scale of the pulses and SS calculations cheap. The influence of reaction heat sources, joule heat source and inlet flow rate on temperature evolution has been investigated. Simulation results indicate that the influence of reaction heat source on electrode temperature is greater than the eletrolyte and most of the joule heat is carried away by the electrolyte flow.

Early and late Holocene paleoenvironmental reconstruction of the Pearl River estuary, South China Sea using foraminiferal assemblages and stable carbon isotopes

Proxy reconstructions of estuarine evolution provide perspectives on regional to global environmental changes, including relative sea-level changes, climatic changes, and agricultural developments. Here, we present a new benthic foraminiferal record along with δ13C and C/N, and lithological data from a sediment core in the Pearl River estuary (Lingding Bay) adjacent to the South China Sea. The core has relatively thick Holocene sediments (>40 m) due to its location in the paleo-valley of the Pearl River. The lithologic and foraminiferal record reveal an evolution in paleoenvironment from fluvial, inner estuary to middle estuary between 11300 and 8100 cal a BP in response to rapid sea-level rise. δ13C and C/N data indicate high freshwater discharge from 10500 to 8100 cal a BP driven by a strong Asian monsoon. The middle Holocene (8100 - 3300 cal a BP) sediment is absent in the core due to subaqueous erosion resulting from the unique geomorphology of the Pearl River Delta. In the late Holocene from 3300 cal a BP to the present, the lithology and foraminiferal assemblages suggest a further evolution from outer estuary, middle estuary channel, to middle estuary shoal, resulting from deltaic progradation under stable relative sea levels. In the last 2000 years, δ13C and C/N values reveal the intensive development of agriculture coupled with the reduction of freshwater input derived from a weakening Asian monsoon.

Single- and Dual-Stream Foam Fractionation of Protein – Exploring a Simple and Effective System to Improve Fundamental Understanding

Abstract In this paper, a single-/dual-stream foam separation device was constructed to simplify the conventional foam fractionation process (CFFP), by minimizing interactions between bubbles. This was expected to help reveal mechanisms in a ‘neat’ way. Results have shown negligible productivity of single-stream foam fractionation (SSFF) for the protein enrichment ratio (E) under conditions tested, whereas dual-stream foam fractionation (DSFF) was established as a reasonable basic unit of CFFP. The influence of DSFF operating conditions, such as the inlet protein concentration and gas velocity, were examined. Calculations of protein concentration and liquid volume were performed via foam thickness measurement, which is very difficult with CFFP. It was evident that the middle drainage channel played a key role in enrichment phenomena. The current DSFF system provides a control case for evaluating principles of foam fractionation. Furthermore, a simple mass-balance model has been proposed to represent the column-wide behavior of DSFF.

Spatio-temporal dynamics of mesozooplankton in the subantarctic Beagle Channel: The case of Ushuaia Bay (Argentina)

The Beagle Channel is the southernmost South American fjord. Although it has been the focus of many biological studies, more in-depth knowledge of plankton and their linkage with environmental variables is lacking. The main objective of this study was to analyse the spatio-temporal variations in mesozooplankton assemblages of Ushuaia Bay, an ecologically and economically relevant area in the middle Beagle Channel, on the basis of samples collected during summer (March), autumn (June), winter (September) and spring (December) 2012 from environmentally different sites. Mesozooplankton composition and abundance exhibited strong seasonal variations, closely associated with the late winter–springphytoplankton bloom. The appendicularian Fritillaria borealis and the cyclopoid copepod Oithona similis were the dominant species during summer and autumn, whereas meroplanktonic larvae and the cladocerans Evadne nordmanni and Podon leuckarti dominated during late winter and spring, respectively. Mesozooplankton abundance was lower at coastal sites receiving freshwater and sewage discharges. Contrary to data reported a decade earlier, small-sized omnivorous species were dominant in Ushuaia Bay in 2012. The increased abundance of these species and decrease in the abundance of calanoids (e.g. Drepanopus forcipatus and Eurytemora americana) may reflect the natural year-to-year variability in phytoplankton structure and water physico-chemical properties. However, since this bay suffers from chronic pollution, other explanations, such as the influence of eutrophication on plankton structure, should also be considered. This study provides a baseline for future research on the effects of natural and anthropogenic environmental change on the Beagle Channel, thereby expanding our knowledge of plankton in subantarctic fjords.

Experimental and numerical research on heat transfer and flow characteristics in two-turn ribbed serpentine channel with lateral outflow

This paper experimentally and numerically investigates the heat transfer and flow characteristics of a two-turn ribbed serpentine channel with lateral outflow. The heat transfer coefficient was measured by a transient liquid crystal technique. Experiments were carried out at Reynolds numbers between 5,000 and 20,000 and rotation numbers of 0 and 0.03. The results indicate that with increasing inlet Reynolds number, the high-Nusselt number regions of the middle and lateral outflow channels gradually move to upstream ribs. The inlet channel shows the highest increase rate of the Nusselt number; whereas, the rate is lowest at downstream turning area. The rotation increases the trailing surface Nusselt number of the inlet and lateral outflow channels, and the increase rate is more prominent for higher values of the Reynolds number. However, the rotation has negative effects on the averaged area Nusselt number of the middle channel in cases of Re ≤ 17,000. The pressure coefficients decrease the in inlet and middle channels along the flow direction; whereas, they slightly increase in the lateral outflow channel. There is a long low-velocity vortex formed in the lateral outflow channel, and the vortex size is considerably reduced by the rotation. Accordingly, the rotation has the most positive effects on the pressure coefficient of the lateral outflow channel.

Size effect on boundary condition at solid-liquid interface in microchannel

The heat transfer in microchannel has attracted considerable attention due to many important applications in biology, chemistry, physics and engineering. When the fluid size shrinks to nanoscale, the energy transport of micro-system is significantly different from the conventional case. It is of great significance to study the size effect on heat transfer in a micro-system. However, there is a large size gap between existing molecular dynamics simulation and experimental measurement, in which the size effect on solid-liquid interfacial thermal resistance is rarely involved. Non-equilibrium molecular dynamics simulation is performed to investigate the heat transfer through the solid-liquid interface. Simple Lennard-Jones (LJ) fluid is simulated as the ultra-thin liquid film in a non-equilibrium simulation system. The liquid film is confined in a nanochannel composed of two solid surfaces. The potential function between solid and liquid atom is represented by a modified LJ function to control the solid-liquid interfaces of different surface wettabilities. We examine the size effect on temperature jump and thermal resistance at the solid-liquid interface. The fluid number density and temperature distribution in the perpendicular direction of solid wall are evaluated. It is found that the liquid atoms near wall are arranged as a solid-like structure. Particularly in the small channel, liquid atoms confined in the channel are affected by two solid walls. However, with the increase of channel height, the liquid atoms in the middle channel move freely, leading to the decrease of the size effect. The simulation results show that the dependence of thermal resistance on microchannel height exhibits two regimes: (i) monotonically increasing dependence for the small channel and (ii) keeping constant thermal resistance for the large channel. These two distinct trends can be explained by phonon vibrational density of states (VDOS) of solid wall and liquid. For the small channel, a stronger confinement of liquid leads to a weaker mismatch in VDOS of solid wall and liquid, thus resulting in a smaller thermal resistance. Whereas, for the large channel, the vibrational coupling between the solid and the liquid atom remains unchanged and the size effect is negligible. The size thresholds of the two regimes of the thermal resistance are both sensitive to the liquid-solid interaction strength, which decreases with solid-liquid interaction increasing. Furthermore, with the increase of the microchannel height, the temperature jump at the solid-liquid interface monotonically decreases and eventually approaches to the non-jump temperature boundary on a macroscopic scale. These findings may help to understand the mechanism of temperature boundary conditions on a microscopic scale and a macroscopic scale and provide a theoretical support for manufacturing new nano-devices.