Microwave-assisted Green Synthesis Research Articles

Exploring the therapeutic potential of microwave-assisted biosynthesized silver nanoparticles using Erica manipuliflora Salisb.: A comprehensive study on anticancer and antibacterial potentials

Silver nanoparticles (AgNPs) continue to attract interest due to their potential applications in biomedicine, especially in relation to their antibacterial and anticancer properties. In this respect, it is important to develop biosynthesis techniques that are environmentally friendly and include new sources. This is the first report on microwave-assisted green synthesis of silver nanoparticles using Erica manipuliflora Salisb. (E.m AgNPs). In this study, the anti-cancer and antibacterial activity of E.m AgNPs and E. manipuliflora extracts were evaluated. Characterization of E.m AgNPs were performed using UV–Vis spectroscopy FT-IR, XRD, SEM and TEM analyses. The UV–Vis absorption spectrum showed the characteristic peak of E.m AgNPs at λmax = 425 nm. The SEM and TEM results indicated that the nanoparticles were spherical and ranged in size from 2.45 to 9.95 nm. The antibacterial results, it was determined that E.m AgNPs (50 mg mL−1) (8.4–21.1 mm ZOI) caused more effective inhibition on both gram positive (Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis) and gram negative (Escherichia coli) bacteria compared to all plant extracts (50 mg mL−1) (8.13–17.16 mm ZOI). Among the cancer (MCF-7 and HT-29) and healthy (HaCaT and HEK-293) cell lines, E.m AgNPs exhibited the highest cytotoxicity against MCF-7 cells (IC50: 87.22 μg mL−1). The hexane extract was the most effective inhibitory extract on cell proliferation of all cell lines and showed the highest cell inhibition in MCF-7 cells (IC50: 43.06 μg mL−1). The results revealed that both E.m AgNPs and E. manipuliflora extracts showed promising antibacterial activity against pathogenic bacterial strains and had potential anticancer activity.

Microwave-assisted green synthesis of silver nanoparticles using Cynoglossum furcatum extract: biomedical evaluation against A431 skin cancer cell line

ABSTRACT The present study was carried out to synthesize silver nanoparticles (AgNPs) from an aqueous leaf extract of Cynoglossum furcatum, which functioned as a capping agent and reducing agent. The synthesized AgNPs have been revealed using a variety of microscopy techniques. The spherical dimension is exploited by electron microscopies, which have an average nanoparticle size of 33 nm. A topographical photograph of Cynoglossum furcatum AgNPs was obtained using atomic force microscopy, demonstrating the reduction of surface irregularity. The MTT assay was employed to evaluate the effect of the plant extract and the AgNPs on the Vero, 3T3 L1, and A431 cell lines. The agar disk diffusion was employed to evaluate the antibacterial efficacy with E.coli, S.typhi, S.aureus, and E.faecalis, as well as the antifungal efficacy against A.niger, C.albicans, A.flavus, and A.fumigates, which exhibited substantial antimicrobial activity.

Microwave-assisted green synthesis and molecular docking study of some new 4-thiazolidinone derivatives as possible bioactive agents

We have developed an economical and environmentally friendly microwave-assisted efficient method for the synthesis of a series of new 4-oxo-thiazolidine derivatives (4a-d). The method involves microwave irradiated cyclocondensation reaction of Schiff bases (3a-d) and thioglycolic acid in drug master file (DMF). 4-oxo-thiazolidine derivatives (4a-d) was also synthe 4-Oxo-Thiazolidine derivatives were also synthesized by conventional heating procedures and consequence were Compared with Microwave assisted green method. The results suggest that microwave-assisted syntheses lead to higher yields within very short reaction times. Antimicrobial screening of all the synthesized compounds was carried out using Gram-positive strains Staphylococcus aureus and Gram-negative strain encompassing Escherichia coli, Proteus vulgaris, and Salmonella typhi. The results were compared with the standard drug chloramphenicol. All the synthesized compounds were subsequently examined using molecular docking experiments against peptide deformylase (PDB ID: 1G2A), which evaluates the binding interaction of ligands with enzyme active sites.. KEYWORDS :Microwave irradiation, Thiazolidinone, Molecular docking, Peptide deformylase, Antimicrobial screening.

Optimization of Microwave-Assisted Green Synthesis of Zinc oxide nanoparticles Using Ocimum americanum and Euphorbia hirta Extracts: In Vitro Evaluation of Antioxidant, Anti-inflammatory, Antibacterial, Cytotoxicity, and Wound Healing Properties

Optimization of Microwave-Assisted Green Synthesis of Zinc oxide nanoparticles Using Ocimum americanum and Euphorbia hirta Extracts: In Vitro Evaluation of Antioxidant, Anti-inflammatory, Antibacterial, Cytotoxicity, and Wound Healing Properties

Microwave-assisted green synthesis of silver nanoparticles using Mitragyna parvifolia bark extract and their biological activities: an economical and environment-friendly approach

ABSTRACT Nowadays, nanotechnology is extensively employed in the medical profession. In this study, we used the aqueous extract of the bark of Mitragyna parvifolia to synthesize silver nanoparticles (AgNPs) by microwave-assisted green synthesis. The synthesis of AgNPs was confirmed by visual color change to brown color and characteristic surface plasmon resonance peak at 432 nm. The hydrodynamic diameter of the AgNPs was 171.81 nm having a zeta potential of −24.14 mV; Fourier Transmission Infrared Spectroscopy confirmed the presence of different functional groups on the NP surface; Scanning Electron Microscope and High-Resolution Transmission Electron Microscopy indicated predominantly circular shape of nanoparticle; Selected Area Electron Diffraction and X-ray Diffraction analyses determined the crystalline structure of AgNPs. Energy-Dispersive X-ray indicated the elemental composition and formation of AgNPs. The AgNPs were screened at different concentrations for antioxidant activity, antimicrobial, and anticancer potential in breast cancer cells (MCF-7 and MDA-MB-231). The AgNPs exhibited remarkable antioxidant, antimicrobial, and anticancer activities. The sedative and antinociceptive activities were also tested on Swiss albino mice, which showed mild sedative and very potent antinociceptive activity. However, detailed mechanistic studies are warranted in the future for clinical application of the AgNPs as a biologically active agent as well as a carrier for drug delivery.

Efficient Green Synthesis and Characterization of Benzil and its Derivatives Using Microwave Irradiation

Abstract: The main aim of the present work was to conduct the one-pot microwave-assisted green synthesis of benzil and its derivatives. Benzil is acknowledged as a pivotal scaffold in the realm of medicinal and organic chemistry, owing to its extensive utilities. Due to the various merits of the green technology approach compared to classical methodology and the provision of sustainable chemistry, this reaction has received renewed interest for preparing benzil derivatives in an environmentally friendly manner with improved yields. We have, herein, presented a highly efficient route for the synthesis of benzil derivatives utilizing acetophenone and benzene derivatives as primary substrates. Notably, this synthesis obviates the necessity for any potentially hazardous catalyst and employs microwave irradiation and iodine green oxidant to facilitate the reaction. All synthesized compounds were characterized by spectroscopic techniques, such as IR, 1H NMR, and mass spectrometry. A green and efficient microwave-assisted synthesis methodology for benzil and its derivatives has been developed using iodine green oxidant. This approach has yielded the desired benzil derivatives with remarkable efficiency, achieving yields ranging from 91% to 97% within a short time of 10-15 minutes; the derivatives have been characterized using spectral techniques, viz., IR, 1H NMR, and mass spectrometry. It is noteworthy that the entire reaction optimization process has been conducted in an environmentally friendly manner, thereby exemplifying a synthetic methodology being both environmentally sustainable and economically viable, compared to conventional techniques.

Microwave-assisted green synthesis of zinc oxide nanoparticles using pistia stratiotes for anticancer and antibacterial applications

Green synthesis techniques have recently become more popular due to the expanding interest in nanotechnology and the need for ecologically friendly synthesis processes. This work examines the environment friendly production of Zinc Oxide Nanoparticles (ZnO NPs) by a microwave-assisted technique, utilizing Pistia Stratiotes leaf extract as a reducing agent. The optical and structural properties of the produced ZnO NPs were analyzed using UV–vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), and x-ray Diffraction (XRD). The findings indicated that the ZnO NPs displayed shapes consisting spherical, flower and sheet, with an average size of 35 nm which was verified by SEM and TEM. The XRD examination confirmed the presence of a hexagonal wurtzite crystalline structure, while the FTIR analysis identified a distinct peak at 578 cm−1, which indicates the stretching mode of Zn-O bonds. The antibacterial and antifungal properties of the substance were evaluated against Staphylococcus Aureus, Escherichia Coli, and Candida Albicans. The results demonstrated notable effectiveness, as indicated by inhibition zones measuring 16–20.4 mm, 17–21.3 mm, and 13–17.2 mm correspondingly. In addition, the ZnO NPs exhibited specific anti-cancer effects on SK-MEL-28 melanoma cell lines, with an IC50 value of 51.05 μg ml−1, suggesting potential uses in therapy. This study demonstrates the feasibility of using microwave-assisted green synthesis to create ZnO NPs with favorable characteristics for biomedical purposes, with a particular focus on sustainable manufacturing of nanomaterials. The results indicate that these ZnO NPs show great potential for application in antibacterial, antifungal, and anticancer therapies, leading to developments in the field of medical nanotechnology.

Microwave-Assisted Green Synthesis of CQDs from Mesosphaerum suaveolens Extract: Photocatalytic Degradation and Anticancer Activity

Microwave-Assisted Green Synthesis of CQDs from Mesosphaerum suaveolens Extract: Photocatalytic Degradation and Anticancer Activity

Microwave-assisted green synthesis of Cassia alata-mediated gold nanoparticles and evaluation of its antioxidant, anti-inflammatory, and antibacterial activities

Microwave-assisted green synthesis of Cassia alata-mediated gold nanoparticles and evaluation of its antioxidant, anti-inflammatory, and antibacterial activities

Green synthesis of nanostructured 1T/2H-MoS2 hybrid phase with polyol solvents and microwave heating

Green synthesis approaches have attracted greatly of attention in recent years since they address the issues associated with sustainability than conventional synthesis methods. New research fields in green nanoscience are being developed as a result of the incorporation of green chemistry principles into nanoscience. In this paper, the successful microwave-assisted green synthesis of MoS2 nanoparticles in a single pot using polyol solvents such as ethylene glycol and glycerol is demonstrated. The coexistence of 1T and 2H phases in MoS2 nanomaterials was determined using advanced techniques such as XRD, Raman, XPS, and TEM images. The highest 1T proportion obtained was 84.5% when compared to the 2H phase. The reaction mechanism and the phase transition between 1T and 2H were described and illustrated. The role of polyol solvents in the practical synthesis of nano MoS2 under microwave heating is also evaluated and explained. Due to the ability of the metallic 1T phase to enhance electrical conductivity, it is believed that hybrid nanostructures exhibit superior electrochemical performance for energy storage and conversion applications.

Microwave-assisted bio-derived bismuth oxybromide (BiOBr) decorated polyaniline nanocomposite for highly sensitive electrochemical determination of endocrine disruptor bisphenol-A

In the presence of polyaniline (PANI), the novel nanocomposites of PANI-BiOBr bismuth oxybromide (BiOBr) were synthesized using a microwave-assisted green synthesis method and used to detect bisphenol A (BPA). By using fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and electrochemical methods, the hybrid PANI-BiOBr nanocomposite was confirmed. The higher conductivity, improve active surface-to-volume ratio, and enhanced electrochemical behaviour of the PANI-BiOBr nanoplatform can be attributed to the synergistic actions of PANI and BiOBr. With a high sensitivity of 11.7 μA (log nM)−1 cm2 and a low limit of detection of 0.19 × 10−9 μM, the constructed PANI-BiOBr sensor exhibits a broad linear range from 0.19 × 10−9 μM to 3.04 × 10−3 μM. In addition, this sensor produced adequate findings with outstanding selectivity, stability, repeatability, and reproducibility. Furthermore, the developed electrochemical PANI-BiOBr nanoplatform is essential in analysing real-world materials to detect BPA. The results obtained from these real sample analyses not only confirmed the effectiveness of the PANI-BiOBr nanocomposite sensor for BPA detection but also underscored its capability to detect other compounds with estrogenic properties, beyond just BPA.

Enhancing the antimicrobial activity of silver nanoparticles against pathogenic bacteria by using Pelargonium sidoides DC extract in microwave assisted green synthesis.

This study presents an optimized microwave-assisted method for the green synthesis of silver nanoparticles (AgNPs) using a root extract obtained from Pelargonium sidoides DC. The influence of temperature, reagent concentration, and irradiation time was systematically investigated to enhance synthesis yield. Characterization techniques including XRD, UV-vis, FTIR, XPS, and zetametry were employed to confirm the successful formation of nanoparticles with a metallic silver core (∼17 nm) functionalized with organic molecules derived from the plant extract. The cytotoxicity of AgNPs was assessed using a cell viability assay, while the Minimum Inhibitory Concentration (MIC) of nanoformulation against pathogenic bacteria, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and the carbapenem-resistant Klebsiella pneumoniae (KPC), was determined using the Broth microdilution method. The nanoformulation synthesized with P. sidoides extract exhibited a dose-dependent response, demonstrating superior antimicrobial efficacy compared to the pure plant extract in most cases. The MIC values ranged from 0.85 to 17.1 μg mL-1, with particularly strong performance against the drug resistant KPC strain. The enhanced antimicrobial effect is attributed to the synergistic action of the metallic silver core and phytochemicals from P. sidoides on the surface of nanoparticles, which also contribute to notable colloidal stability of AgNPs at physiological pH levels.

Novel tetraaza macrocyclic Schiff base complexes of bivalent zinc: microwave-assisted green synthesis, spectroscopic characterization, density functional theory calculations, molecular docking studies, in vitro antimicrobial and anticancer activities.

In the present manuscript, novel macrocyclic Schiff base complexes [Zn(N4MacL1)Cl2-Zn(N4MacL3)Cl2] were synthesized by the reaction of ZnCl2 and macrocyclic ligands (N4MacL1-N4MacL3) derived from diketone and diamines under microwave irradiation method and conventional method. The structures of the obtained complexes were identified by various spectrometric methods such as Fourier transformation infra-red (FT-IR), nuclear magnetic resonance (NMR), high-resolution mass spectrometry (HR-MS), powder X-ray diffraction, molar conductivity, and UV-vis. The structures of the synthesized compounds were optimized by using the def2-TZV/J and def2-SVP/J Coulomb fitting basis sets at B3LYP level in density functional theory (DFT) calculations. The macrocyclic Schiff base complexes exhibited higher activities against Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus), Gram-negative bacteria (Escherichia coli and Xanthomonas campestris), and fungal strains (Fusarium oxysporum and Candida albicans) in comparison to macrocyclic Schiff base ligands. Furthermore, the newly synthesized macrocyclic compounds were assessed for their anticancer activity against three cell lines: A549 (human alveolar adenocarcinoma epithelial cell line), HT-29 (human colorectal adenocarcinoma cell line), and MCF-7 (human breast adenocarcinoma cell line) using the MTT assay. The obtained results showed that the macrocyclic complex [Zn(N4MacL3)Cl2] displayed the highest cytotoxic activity (2.23 ± 0.25µM, 6.53 ± 0.28µM, and 7.40 ± 0.45µM for A549, HT-29, and MCF-7 cancer cell lines, respectively). Additionally, molecular docking investigations were conducted to elucidate potential molecular interactions between the synthesized macrocyclic compounds and target proteins. The results revealed a consistent agreement between the docking calculations and the experimental data.

Ilmenite-Grafted Graphene Oxide as an Antimicrobial Coating for Fruit Peels.

Postharvest loss is a significant global challenge that needs to be urgently addressed to sustain food systems. This study describes a simple microwave-assisted green synthesis method in developing a nanohybrid material combining natural ilmenite (FeTiO3) and graphene oxide (GO) as a promising antimicrobial fruit peel coating to reduce postharvest loss. The natural ilmenite was calcined in an inert environment and was mixed with GO in a microwave reactor to obtain the nanohybrid. The nanohybrid was then incorporated into an alginate biopolymer to form the fruit coating. Microscopic images revealed successful grafting of FeTiO3 nanoparticles onto the GO sheets. Spectroscopic measurements of Raman, X-ray photoemission, and infrared provided insights into the interactions between the two matrices. The optical band gap calculated from Tauc's relation using UV-vis data showed a significant reduction in the band gap of the hybrid compared to that of natural ilmenite. The antimicrobial activity was assessed using Escherichia coli, which showed a substantial decrease in colony counts. Bananas coated with the nanohybrid showed a doubling in the shelf life compared with uncoated fruits. Consistent with this, the electronic nose (E-nose) measurements and freshness indicator tests revealed less deterioration of the physicochemical properties of the coated bananas. Overall, the results show promising applications for the ilmenite-grafted GO nanohybrid as a food coating capable of minimizing food spoilage due to microbial activity.

The effect of fish collagen on the silver nanoparticles sizes and shapes using modified microwave-assisted green synthesis method and their antibacterial activities

This work aimed to produce silver nanoparticles (AgNPs) by efficient green synthesis techniques, namely rapid green synthesis and modified microwave-assisted green synthesis methods. The study used fish scale collagen (FsCol) as a stabilizer to assess its impact on the dimensions and configurations of AgNPs. Four samples were prepared with varying concentrations of FsCol. The synthesized AgNPs were characterized using Ultraviolet–visible (UV–vis) spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray analysis (EDX), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray diffraction analysis (XRD), Dynamic Light Scattering (DLS), and Transmission electron microscopy (TEM) techniques. The obtained sizes are as follows: 85 ± 15 nm, 70 ± 10 nm, 50 ± 10 nm, and 28–40 nm. The UV–vis spectroscopy revealed a shift in the absorbance peaks from 400 to 446 nm. The SEM method showed a spherical form in all of the samples. The element silver was detected in the EDX examination, along with the presence of oxygen (O) and carbon (C). The FTIR analysis revealed that the peaks seen at 3307 cm−1 were attributed to the stretching of O–H bonds, while the mountain at 1638 cm−1 belonged to the extension of N–H bonds (amide A). Additionally, the band observed at 1638 cm−1 indicated the presence of CO bonds (amide I).The 2140 cm−1 and 1302 cm−1 peaks may be attributed to the C2H2 group present in the plant components and the N–H bending (Amide III), respectively. The XRD pattern indicates that the synthesis process resulted in the formation of crystalline AgNPs. The particle sizes measured using DLS were 121 nm, 96.36 nm, 82.3 nm, and 48.50 nm. The TEM approach revealed that all samples had a spherical morphology with varying sizes: 80–100 nm, 50–80 nm, 40–60 nm, and 28–42 nm. The synthesized AgNPs were tested for their antibacterial properties against the pathogenic pathogens Escherichia coli (E.coli) and Staphylococcus aureus (S. aureus). The influence of AgNPs on bacteria was amplified as the particle size decreased, resulting in a larger inhibitory zone for the smaller particles.

Microwave-assisted green synthesis of silver nanoparticles and chitosan nanocomposites for the removal of reactive blue-19

Microwave-assisted green synthesis of silver nanoparticles and chitosan nanocomposites for the removal of reactive blue-19

Green synthesis, structure-activity relationships, in silico molecular docking, and antifungal activities of novel prenylated chalcones.

A series of 16 novel prenylated chalcones (5A-5P) was synthesized by microwave-assisted green synthesis using 5-prenyloxy-2-hydroxyacetophenone and different benzaldehydes. Comparisons were also performed between the microwave and conventional methods in terms of the reaction times and yields of all compounds, where the reaction times in the microwave and conventional methods were 1-4min and 12-48h, respectively. The synthesized compounds were characterized using different spectroscopic techniques, including IR, 1H-NMR, 13C-NMR, and LC-HRMS. The antifungal activities of all compounds were evaluated against Sclerotium rolfsii and Fusarium oxysporum under in vitro conditions and were additionally supported by structure-activity relationship (SAR) and molecular docking studies. Out of the 16 compounds screened, 2'-hydroxy-4-benzyloxy-5'-O-prenylchalcone (5P) showed the highest activity against both S. rolfsii and F. oxysporum, with ED50 of 25.02 and 31.87mg/L, respectively. The molecular docking studies of the prenylated chalcones within the active sites of the EF1α and RPB2 gene sequences and FoCut5a sequence as the respective receptors for S. rolfsii and F. oxysporum revealed the importance of the compounds, where the binding energies of the docked molecules ranged from -38.3538 to -26.6837 kcal/mol for S. rolfsii and -43.400 to -23.839kcal/mol for F. oxysporum. Additional docking parameters showed that these compounds formed stable complexes with the protein molecules.

Novel Schiff base derivatives containing 4,5-disubstituted thiazole as potential antibiofilm, anti-inflammatory and antioxidant agents: Green synthesis, molecular docking and ADME analysis

We report the microwave-assisted (MW) green synthesis of some novel 4,5 disubstituted thiazole-containing Schiff base derivatives (SBs) as antibiofilm, anti-inflammatory, and antioxidant agents. Spectroscopic techniques were used to confirm the formation of synthesized compounds. In-vitro antibiofilm and antifungal activity of synthesized molecules were evaluated against the Candida albicans strain (MTCC 227) by broth dilution method. Among the tested molecules, 3a showed excellent inhibition of both biofilm formation and planktonic growth at much lower MIC50 values (5.56 μg/mL and 1.20 μg/mL respectively) than other derivatives. Moreover, the spot assay of compound 3a showed fungicidal activity (100% cell death) for biofilm formation and planktonic growth at the lowest MFC values (10 μg/mL and 3.12 μg/mL respectively) compared to the standard drug fluconazole. In addition, the biofilm inhibition was further confirmed by morphological study through FESEM analysis. The anti-inflammatory study of SBs by protein denaturation method indicates that compound 3a (63.19%) was the most active anti-inflammatory agent at a lower concentration of 100 μg/mL in the series than the standard ibuprofen (42.36%). Whereas, the antioxidant study of SBs by the DPPH scavenging method shows compound 3j (IC50 = 436.10 μg/mL) was the more potent antioxidant agent as compared to other tested compounds and standard drug ascorbic acid (IC50 = 626.99 μg/mL). Furthermore, the molecular docking study of SBs with the active sites of sterol 14-α demethylase CYP51 enzyme of (PDB:5TZ1) C. albicans shows strong binding properties, supporting the experimental results of inhibition of biofilm formation. Additionally, the ADME characteristic of SBs demonstrates their potential for development as oral medication candidates.

Microwave-assisted green synthesis of silver nanoparticles using turnip root (Brassica rapa subsp. Rapa) extract and their antibacterial efficacy

Biosynthesised silver nanoparticles (AgNPs), especially those synthesised by plant extracts, are extensively utilised in the fields of pharmacy and medicine due to their ability to exhibit a wide range of biological functions. The current study is focused on the green synthesis of silver nanoparticles from various concentrated silver nitrate solutions using turnip root extract as a reducing and stabilising agent and to assess the in vitro antibacterial efficacy of these AgNPs. For the study, we employed microwave-assisted reduction to examine the impact of microwave energy on the resulting AgNPs in comparison to the conventional heating method. X-ray diffraction analysis revealed the crystalline nature of the as-prepared AgNPs, while field emission-scanning electron microscopy (FE-SEM) showed the majority of spherical-shaped AgNPs had an average grain size of 47.5 nm. Energy-dispersive x-ray spectroscopy (EDX) indicates the high weight percentage of the produced AgNPs than for extract residue, especially for the AgNPs that are prepared with microwave assistance. Fourier transform infrared spectroscopy (FTIR) was used to reveal the functional groups that are related to many phytochemical compounds, such as alkaloids, flavonoids, and terpenoids, which act as reducing and stabilizing agents. High zeta potential measures (−22.77 and −38.83 mv) approved the high stability of the synthesised AgNPs as higher zeta potentials typically correlate with greater stability. The antibacterial activity behaviour of the produced NPs against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria was significantly acceptable for both heating techniques, as observed from the measured inhibition zone.

Phyto-fabrication of CuO–Co3O4 nanocomposites for antibacterial, photocatalytic and antioxidant evaluations: An in-vitro investigation

In this current investigation, we elucidated the microwave-assisted green synthesis of 90:10, 80:20, and 70:30 CuO–Co3O4 nanocomposites (NCs) from Ipomoea carnea leaf extract, manifesting their remarkable antibacterial, photocatalytic, and antioxidant capabilities. The spectroscopic techniques of XRD, SEM, EDX, TEM, FTIR, and UV–visible spectroscopy were employed to validate the formation of the synthesized NCs. SEM and TEM images revealed distinctive snowball-rod and distorted spherical structures in the synthesized nanocomposites, with average particle sizes of 33.36, 26.17, and 29.10 nm. The estimated energy band gap values were 1.20, 1.40, and 1.50 eV for the respective nanocomposites. The well diffusion method was utilized to explore the zone of inhibition (ZOI) against bacteria, namely Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. The nanocomposites, specifically the 90:10 and 80:20 CuO–Co3O4 blends, exhibited a noteworthy Zone of Inhibition (ZOI) during testing against B. subtilis, with approximate values of 34.76 ± 0.23 and 33.33 ± 0.32 mm, respectively. Furthermore, the photocatalytic potential has been investigated against the dyes Malachite green (MG) and Congo red (CR). Notably, the 90:10 NCs displayed an exceptional photodegradation rate of 97.69 % for MG and 95 % for CR within a mere 45 min. Moreover, the 70:30 NCs exhibited a commendable radical scavenging activity of approximately 97.53 % at a concentration of 800 μg/mL, while ascorbic acid, used as a standard, displayed a scavenging activity of 98.63 %. These investigations unveil the environmentally friendly, innovative, and cost-effective attributes of CuO–Co3O4 NCs derived from Ipomoea carnea leaf extract, which demonstrated excellent antibacterial, photocatalytic, and antioxidant properties.