Blue Whiting Research Articles

Escape of hake (Merluccius merluccius), horse mackerel (Trachurus trachurus) and blue whiting (Micromesistius poutassou) in codends with shortened lastridge ropes

Diamond meshes in trawl codends have limited openness, which reduces escape opportunities for roundfish. Shortening the lastridge ropes (LR) attached to codend selvedges can increase the availability of open meshes resulting in higher chances of escape. However, this availability does not imply optimal mesh openness, nor does it guarantee use. We estimate the escape probability of hake, horse mackerel and blue whiting through a 20% shortened LR codend and a standard codend, and quantify the contribution of different mesh opening angles (OAs) to their size selectivity. The results confirm that high OAs increase escape opportunities for all species. However, shortened LR only improved size selectivity significantly for horse mackerel and blue whiting. This difference between species may be related to behavioural differences. The mesh openness achieved with 20% shortened LR was below that necessary to obtain optimal escape opportunities for these species. The study highlights the relevance of considering fish morphology and behaviour to optimally exploit size selectivity when designing shortened LR codends.

A catch limitation device to avoid excessive catches in the blue whiting (Micromesistius poutassou) Northeast Atlantic pelagic trawl fishery

Blue whiting (Micromesistius poutassou) is harvested in the Northeast Atlantic by a multinational fleet of pelagic trawlers. Occasionally, vessels take catches which exceed their remaining holding capacity and in extreme cases large catches cause codends to burst, resulting in spill of catch. To control catch quantity, a catch limitation system was developed and tested. The system consists of three components: 1) escape opening(s) in front of the codend to release excess fish, 2) a fish lock to prevent loss of fish through the escape opening(s) during haulback and at the surface, and 3) a choking unit to match codend capacity to the desired size of catch. Blue whiting escaped through both longitudinal slots and large (≥ 2 m) meshes in front of the codend. However, video observations showed that with large meshes in the upper panel, large amounts of blue whiting escaped long before the codend was full. Therefore, a design with large openings in the bottom was combined with longitudinal slots in the side and top panels. Two fish locks were tested: an oblique netting panel designed to seal off the codend when vessel speed reduce during haulback, and a cylinder of netting with a choking rope that closed it after the codend was filled. Both fish lock designs inhibited release of fish during haulback and at the surface, but the attachment of the constricting rope in the cylinder frequently broke. The choking unit consisted of a depth triggered releaser connected to a strap of rope wrapped around the codend. Mechanical releasers with factory-set depth trigger and electronic versions were tested. Both freed the choke point as intended, but occasionally at unpredictable depth. A reliable relationship (r2 = 0.94) was attained between codend choking position and catch amount, demonstrating that codend capacity could be adjusted to achieve target catch quantities. Our work shows that controlling catch quantity in the blue whiting pelagic trawl fishery can be achieved effectively through relatively simple modifications to the codend section Future work is needed to optimize the fish lock design and ensure the codend choking rope releasers trigger reliably and at the proper depth.

Wild fish and seafood species in the western Mediterranean Sea with low safe mercury concentrations

A total of 1345 specimens belonging to 58 different species of wild fish and seafood from the western Mediterranean Sea were analyzed to assess total mercury levels and to estimate which species meet the EU recommendations for human consumption (0.5 μg g−1 ww) in all cases. All fish species were caught off the Mediterranean coasts and intended for human consumption. All specimens were collected from local markets located in Spain, Italy and France that sell fish caught by local fishermen (Eivissa, Menorca, Mallorca, Alacant, L'Ampolla, Ametlla de Mar, Marseille, Genoa, Civitavecchia, Alghero) at different time periods. Mercury concentrations were measured by thermal decomposition-gold amalgamator-atomic absorption spectrometry.Only thirteen species were found that did not exceed 0.5 μg g−1 ww in any specimen analyzed. These safe species were sardines (Sardina pilchardus), anchovies (Engraulis encrasicolus), blue whiting (Micromesistius poutassou), picarel (Spicara smaris), blackspot seabream (Pagellus bogaraveo), gilthead seabream (Sparus aurata), pearly razorfish (Xyrichtys novacula), surmullet (Mullus surmuletus), painted comber (Serranus scriba), brown meagre (Sciaena umbra), salema (Sarpa salpa), common dolphinfish (Coryphaena hippurus) and squid (Loligo vulgaris). These species occupy different trophic levels, have different lengths and average weights, but show a low mercury concentration than others living in the same environments.Potential human consumption of these species as sole source of fish would imply estimated weekly intakes representing between 49% and 70% of the recommended provisional tolerable weekly intake of methylmercury in the worst case.Health authorities should pay specific attention to species that do not meet EU thresholds and make appropriate precautionary health recommendations, especially for pregnant women and children.

The effect of grid size on catch efficiency and by-catch in the demersal trawl fishery for Norway pout (Trisopterus esmarkii)

Norway pout (Trisopterus esmarkii) is caught with large trawls with small meshed codends, inevitably causing the fishery to have large by-catch issues. To reduce the amount of by-catch, a rigid sorting grid was made compulsory in 2010. However, there is still a severe by-catch issue, as well as the loss of target species because of the grid. A possible cause might be clogging of the grid, which could be solved by increasing the grid area. Therefore, this study compared the size selectivity of by-catch species and target species in a double-trawl configuration in which one trawl was equipped with a standard grid (6.30 m2), and the other trawl was equipped with a grid that had a 50% larger surface area (9.45 m2). The results demonstrated that the size selectivity and catch efficiency of the target species were unaffected; neither was there any significant difference between the two grids in terms of wanted by-catch species [blue whiting (Micromesistius poutassou), horse mackerel (Trachurus trachurus), and greater argentine (Argentina silus)]. However, the larger grid caught significantly more unwanted by-catch species [haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus), and hake (Merluccius merluccius)]. Approximately one-third of the accumulated catches comprised the target species, one-third of wanted by-catch species, and one-third of unwanted by-catch species, demonstrating the by-catch challenges in this fishery. Simultaneously, none of the by-catch limits were exceeded.

Quantification of trophic interactions in the Norwegian Sea pelagic food-web over multiple decades

Abstract While ecosystem-based fisheries management calls for explicit accounting for interactions between exploited populations and their environment, moving from single species to ecosystem-level assessment is a significant challenge. For many ecologically significant groups, data may be lacking, collected at inappropriate scales or be highly uncertain. In this study, we aim to reconstruct trophic interactions in the Norwegian Sea pelagic food-web during the last three decades. For this purpose, we develop a food-web assessment model constrained by existing observations and knowledge. The model is based on inverse modelling and is designed to handle input observations and knowledge that are uncertain. We analyse if the reconstructed food-web dynamics are supportive of top-down or bottom-up controls on zooplankton and small pelagic fish and of competition for resources between the three small pelagic species. Despite high uncertainties in the reconstructed dynamics, the model results highlight that interannual variations in the biomass of copepods, krill, amphipods, herring, and blue whiting can primarily be explained by changes in their consumption rather than by predation and fishing. For mackerel, variations in biomass cannot be unambiguously attributed to either consumption or predation and fishing. The model results provide no support for top-down control on planktonic prey biomass and little support for the hypothesised competition for resources between the three small pelagic species, despite partially overlapping diets. This suggests that the lack of explicit accounting for trophic interactions between the three pelagic species likely have had little impact on the robustness of past stock assessments and management in the Norwegian Sea.

TrophicCS: Spatialized trophic data of the Celtic Sea continental shelf food web.

Understanding the dynamics of species interactions for food (prey-predator, competition for resources) and the functioning of trophic networks (dependence on trophic pathways, food chain flows, etc.) has become a thriving ecological research field in recent decades. This empirical knowledge is then used to develop population and ecosystem modeling approaches to support ecosystem-based management. The TrophicCS data set offers spatialized trophic information on a large spatial scale (the entire Celtic Sea continental shelf and upper slope) for a wide range of species. It combines ingested prey (gut content analysis) and a more integrated indicator of food sources (stable isotope analysis). A total of 1337 samples of large epifaunal invertebrates (bivalve mollusks and decapod crustaceans), zooplankton, fish, and cephalopods, corresponding to 111 taxa (94% determined at the species level), were collected and analyzed for stable isotope analysis of their carbon and nitrogen content. Samples were collected between 2014 and 2016, mostly during the month of November and between 57 and 516 m depth. Sample size varied between taxa (from 1 to 52), with 98 taxa having at least three samples. The gut contents of 1027 fish belonging to 10 commercially important species: black anglerfish (Lophius budegassa), white anglerfish (Lophius piscatorius), blue whiting (Micromesistius poutassou), cod (Gadus morhua), haddock (Melanogrammus aeglefinus), hake (Merluccius merluccius), megrim (Lepidorhombus whiffiagonis), plaice (Pleuronectes platessa), sole (Solea solea), and whiting (Merlangius merlangus) were analyzed. Sampling occurred in November 2014 and 2015. The gut content data set contains the occurrence of prey in gut, identified to the lowest taxonomic level possible. No prey were assigned for 274 empty gut contents. To consider potential ontogenetic diet changes, a large size range was sampled for each species. The TrophicCS data set was used to improve understanding of trophic relationships and ecosystem functioning in the Celtic Sea. Data are released under a CC-BY-NC-SA license, and please cite this paper when reusing the data.

Effect of Frying Conditions on Textural Parameters, Color and Proximate Composition of Fried Fish Nuggets from Blue Whiting (Micromesistius Poutassou)

ABSTRACT Fish is a good source of protein and plays an important role in fighting hunger and malnutrition. Effect of frying temperature (150°C, 160°C, 170°C), frying time (4, 5, 6 min) and thickness (1.5, 2.5, 3.5 cm) on proximate composition, texture and color parameters of fish nuggets from blue whiting was evaluated. Box-Behnken design was used to analyze and optimize frying conditions. Frying temperature significantly (p < .05) affected protein, ash and springiness positively but negatively affected crude fiber and hardness. Frying time had significant effect (p < .05) on protein, ash and springiness positively but negatively affected crude fiber. Thickness significantly (p < .05) affected crude fiber and stringiness positively while protein, ash, oil content, springiness, cohesiveness and gumminess was significantly (p < .05) affected negatively. Consumers desired taste of the fish nuggets more than other sensory attributes evaluated. Optimized frying temperature of 157°C, frying time 4 min and thickness of 2.3 cm were obtained with 61% desirability.

Severe winter conditions improve recruitment success of blue whiting (Micromesistius poutassou), a temperate water fish species, in the NW Mediterranean sea

Fish recruitment variability results from a complex mix of biological and physical processes, and their interactions, acting in the early life stages. In this study, we aim to investigate the environmental factors driving the recruitment success of blue whiting (Micromesistius poutassou) in the NW Mediterranean through reconstruction of early life history traits derived from otoliths microstructure analysis. To this purpose, the study characterizes the oceanographic conditions in winter-spring of two contrasted years, 2017 and 2018, and relates them to the onset and duration of the spawning, growth performance and condition of blue whiting recruits. Winter 2017 was mild, with a short period of cold temperatures and limited winter vertical mixing. In winter 2018,temperatures reached lower values and extended for a longer period with intense vertical mixing and dense water cascading down the continental slope at the end of February. These different conditions were mirrored in the subsequent phytoplanktonic bloom, which in 2018 occurred later and was more intense than in 2017, extending over a longer period and occupying a wider area. The reproductive period of blue whiting was linked to the duration of low winter temperatures (∼13 °C), shorter in 2017 than in 2018 (∼40 and ∼ 60 days respectively). While in 2017 all individuals were born in a relatively short period of time, and under similar environmental conditions, in 2018 the hatching period presented two differentiated peaks, separated by a period of relatively low hatchings at the end of February. This gap corresponded to dense water cascading and intense vertical mixing events, suggesting that these phenomena limited the survival of eggs and larvae. The few individuals hatched in this period showed the lowest growth rates (maximum values ∼ 1.5 mm TL day−1), which would be related with the poor trophic environment during these events of intense mixing. Conversely, in both years, higher growth rates corresponded to the individuals born just before the phytoplankton bloom (attaining ∼ 2 mm TL day−1). Recruits of 2018 showed better condition than those born the previous year which would be associated with the higher primary production detected in spring 2018. The recruitment strength in 2018, estimated from landings, was also much higher indicating that severe winter conditions translate into improved recruitment of a temperate water fish in the NW Mediterranean Sea.

Microbiological Assessment of Smoked Fishes from Various Processing Points in Ado Ekiti Metropolis

The purpose of the research is to isolate and characterize the bacteria present in smoked fish sold in various processing site in ado Ekiti Metropolis. Fifty (50) samples of five (5) species of fish (Mackerel:Somber scomber, Blue whiting: Micromesistius poutassou, Catfish: Clarias gariepinus Herrings: Clupea harengus, Horse mackerel: Trachurus trachurus ) were obtained from fifteen (15) different processing site in different location in Ado-Ekiti. The study results showed the total heterotrophic bacteria count which ranges from 0.30 x104 – 3.68 x 104 cfu/g, the highest colony count was examined from the panla (Blue whiting {Micromesistius poutassou}.The bacteria organisms isolated and identified in the smoked fish were Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus sp, Bacillus subtilis, Escherichia coli, Proteus mirabilis and Staphylococcus aureus. The study showed that though smoking helps in inhibiting activities of bacteria, however, when not properly carried out, bacterial growth and activities still lead to the deterioration of the fish. Due to public health implication, the state of smoked fish should be paid proper attention by the processors and consumers for their safety through proper processing, storage and handling procedures.

Microplastics Risk into a Three-Link Food Chain Inside European Hake

Microplastics (MPs) are increasing in the marine environment as well as inside marine organisms, having an important effect on biological diversity. The trophic transfer of MPs was demonstrated under laboratory conditions, but this study is based on the analysis of preys found in stomach contents. MPs from Merluccius merluccius individuals caught in the Cantabrian Sea and preys inside their guts (blue whiting, and northern krill inside blue whiting) were analyzed. MPs with different chemical composition occurred inside every hake and their preys, with different damages, from aquatic life hazards with long lasting effects, to allergic skin reactions and respiratory irritation, not only for aquatic species and fishing resources, but also for humans through hake consumption. The similarity of MPs profiles from gills and seawater samples would support seawater as the main source of gill microplastics. The MPs profile of hake GIT was similar to that of hake preys inside. Despite the small sample size, the presence of MPs in all the tissues analyzed of hakes and their preys, together with the evidence of hazard compositions of some of them, highlights the need for policies and actions to reduce plastic and microplastic production and consumption.

Fishery dynamics of blue whiting, Micromesistius poutassou, a highly discarded bycatch species in the NW Mediterranean Sea

This study characterized the fishery dynamics of blue whiting, a bycatch species of the bottom trawl Norway lobster fishery, in the NW Mediterranean. Landings and effort data over the last two decades were combined with current information on the spatial distribution of effort and catch, and with the size and age structure of blue whiting over the year. Recruitment to the demersal habitat occurred from 5 cm total length (TL) over the shelf break in spring (April-May), when discards showed the highest values (&gt;95% of the catch). Adults were located over the upper slope, and large individuals (≥26 cm TL) were abundant during the reproductive period (January-March). A decline in landings has been observed in the last decade, probably related to the absence of intense deep convection processes in winter since 2013, which favour recruitment of the species. The effect of exploitation on the decrease in landings cannot be disregarded considering that the bulk of the catch is made up of immature (age 0) individuals, and the presence of old individuals is very low.

Changes in Protein and Non-Protein Nitrogen Compounds during Fishmeal Processing—Identification of Unoptimized Processing Steps

Quality changes of protein and non-protein nitrogen compounds during industrial fishmeal processing of fatty pelagic species (mackerel/herring rest material blend, MHB) and lean fish (whole blue whiting, BW) were studied to identify processing steps that require optimization to allow production of products for human consumption. Samples from protein-rich processing streams throughout the fishmeal production were analyzed for proximate composition, salt soluble protein content (SSP), biogenic amines (BA), total volatile basic nitrogen (TVB-N), trimethylamine (TMA), and dimethylamine (DMA). Mass flows throughout processing were balanced based on the total mass and proximate composition data. The quality of the final fishmeal products was highly dependent on the fish species being processed, indicating that the processes require optimization towards each raw material. The chemical composition changed in each processing step, resulting in different properties in each stream. Most of the non-protein nitrogen compounds (including BA, TVB-N, TMA, and DMA) followed the liquid streams. However, the concentrate contributed less than 20% to the produced fishmeal quantity. Mixing of this stream into the fishmeal processing again, as currently carried out, should thus be avoided. Furthermore, the cooking, separating, and drying steps should be optimized to improve the water and lipid separation and avoid the formation of undesired nitrogen compounds to produce higher-value products intended for human consumption.

The origins of the killer whale ecomorph

The killer whale (Orcinus orca) and false killer whale (Pseudorca crassidens) are the only extant cetaceans that hunt other marine mammals, with pods of the former routinely preying on baleen whales >10 m in length and the latter being known to take other delphinids.1-3 Fossil evidence for the origins of this feeding behavior is wanting, although molecular phylogenies indicate that it evolved independently in the two lineages.4 We describe a new extinct representative of the killer whale ecomorph, Rododelphis stamatiadisi, based on a partial skeleton from the Pleistocene of Rhodes (Greece). Five otoliths of the bathypelagic blue whiting Micromesistius poutassou are associated with the holotype, providing unexpected evidence of its last meal. The evolutionary relationships of R. stamatiadisi and the convergent evolution of killer whale-like features were explored through a broad-ranging phylogenetic analysis that recovered R. stamatiadisi as the closest relative of P. crassidens and O. orca as the only living representative of a once diverse clade. Within the clade of Orca and kin, key features implicated in extant killer whale feeding, such as body size, tooth size, and tooth count, evolved in a stepwise manner. The tooth wear in Rododelphis and an extinct species of Orcinus (O. citoniensis) are consistent with a fish-based diet, supporting an exaptative Pleistocene origin for marine mammal hunting in both lineages. If correct, predation by the ancestors of Pseudorca and Orca did not play a significant role in the evolution of baleen whale gigantism. VIDEO ABSTRACT.

Natural variability and potential use of melanomacrophage centres as indicators of pollution in fish species from the NW Mediterranean Sea

The present study provides a baseline assessment of the prevalence and densities of splenic melanomacrophage centres (MMCs) in 18 fish species from the NW Mediterranean Sea related to spatiotemporal and environmental factors and fish traits. Their correlation with other established health indicators, such as body condition indices (condition factor, hepatosomatic and gonadosomatic indices), parasite community descriptors and histological assessment of target organs (gills, liver and spleen) is also assessed. Despite MMCs variability is mainly attributed to the species identity and fish size, their potential use as generic biomarkers of health condition is pointed out for certain species (e.g. Spicara maena and Micromesistius poutassou) in which an increased response was identified, and whose potential drivers are discussed. Most importantly, present results provide a comprehensive assessment of MMCs in the fish community for future studies in the area.

Blue Whiting (Micromesistius poutassou) Protein Hydrolysates Increase GLP-1 Secretion and Proglucagon Production in STC-1 Cells Whilst Maintaining Caco-2/HT29-MTX Co-Culture Integrity.

Inducing the feeling of fullness via the regulation of satiety hormones presents an effective method for reducing excess energy intake and, in turn, preventing the development of obesity. In this study, the ability of blue whiting soluble protein hydrolysates (BWSPHs) and simulated gastrointestinal digested (SGID) BWSPHs, to modulate the secretion and/or production of satiety hormones, such as glucagon-like peptide-1 (GLP-1), cholecystokinin (CCK) and peptide YY (PYY), was assessed in murine enteroendocrine STC-1 cells. All BWSPHs (BW-SPH-A to BW-SPH-F) (1.0% w/v dw) increased active GLP-1 secretion and proglucagon production in STC-1 cells compared to the basal control (Krebs–Ringer buffer) (p < 0.05). The signaling pathway activated for GLP-1 secretion was also assessed. A significant increase in intracellular calcium levels was observed after incubation with all BWSPHs (p < 0.05) compared with the control, although none of the BWSPHs altered intracellular cyclic adenosine monophosphate (cAMP) concentrations. The secretagogue effect of the leading hydrolysate was diminished after SGID. Neither pre- nor post-SGID hydrolysates affected epithelial barrier integrity or stimulated interleukin (IL)-6 secretion in differentiated Caco-2/HT-29MTX co-cultured cells. These results suggest a role for BWSPH-derived peptides in satiety activity; however, these peptides may need to be protected by some means to avoid loss of activity during gastrointestinal transit.

Exploring the Potential of Forecasting Fish Distributions in the North East Atlantic With a Dynamic Earth System Model, Exemplified by the Suitable Spawning Habitat of Blue Whiting

Local oceanographic variability strongly influences the spawning distribution of blue whiting (Micromesistius poutassou). Here, we explore the potential of using a dynamic Earth System Model (ESM) to forecast the suitable spawning habitat of blue whiting to assist management. Retrospective forecasts of temperature and salinity with the Max Planck Institute ESM (MPI-ESM) show significant skill within blue whiting’s spawning region and spawning depth (250–600 m) during the peak months of spawning. While persistence forecasts perform well at shorter lead times (≤2 years), retrospective forecasts with MPI-ESM are clearly more skilful than persistence in predicting salinity at longer lead times. Our results indicate that retrospective forecasts of the suitable spawning habitat of blue whiting based on predicted salinity outperform those based on calibrated species distribution models. In particular, we find high predictive skill for the suitable spawning habitat based on salinity predictions around one year ahead in the area of Rockall-Hatton Plateau. Our approach shows that retrospective forecasts with MPI-ESM show a better ability to differentiate between the presence and absence of suitable habitat over Rockall Plateau compared to persistence. Our study highlights that physical-biological forecasts based on ESMs could be crucial for developing distributional forecasts of marine organisms in the North East Atlantic.

DNA metabarcoding suggests dietary niche partitioning in the Adriatic European hake

The Northern Adriatic Sea (FAO Geographical Sub-Area 17) is one of the most productive fishing areas of the Mediterranean Sea and it includes a broad diversity of habitats. In the Northern Adriatic basin, the Pomo Pit (200–273 m of depth) is one of the most important areas of aggregation for some demersal stocks shared in the Adriatic Sea and it is an important spawning/nursery area of the European hake (Merluccius merluccius). Through a metabarcoding approach we investigated the feeding habits of European hake, both inside and outside the Pomo Pit, and their temporal variability comparing samples collected in 2016 and 2014. Our analyses proved the presence of an ontogenetic shift from a diet based mainly on crustaceans in juveniles to a more piscivorous feeding behaviour in adult hakes and suggested the presence of a specific niche partitioning and food preferences between hakes living inside and outside the Pomo Pit. The main differences among adult hakes refer to the presence of molluscs in the stomachs of hakes collected within the Pomo Pit and the presence of high depth prey species (i.e., Micromesistius poutassou). Metabarcoding revealed the relevant ecological role played by the Pomo Pit in M. merluccius feeding behaviour and ontogenetic development, promoting a careful ecosystem-based management of fisheries in this area through focused conservation measures.

Optimizing size selectivity and catch patterns for hake (Merluccius merluccius) and blue whiting (Micromesistius poutassou) by combining square mesh panel and codend designs

Gear modifications in fisheries are usually implemented to obtain catch patterns that meet management objectives. In the Basque bottom trawl fishery, gear regulations include the use of a square mesh panel (SMP) placed at the top panel of the extension piece of the trawl to supplement diamond mesh codend selectivity. However, the catch patterns obtained with this combination have raised concern among scientists and authorities. This study combines new data on different SMP and codend designs with existing data from the literature to produce new results that are applied to predict the size selectivity and catch patterns of different gear combinations for a variety of fishing scenarios. A systematic approach based on the concept of treatment trees was outlined and applied to depict the effect of individual and combined gear design changes on size selectivity and catch patterns for hake (Merluccius merluccius) and blue whiting (Micromesistius poutassou). This approach led to identification of the gear combination with the most appropriate exploitation pattern for these two species and improved the readability and interpretation of selectivity results. The results demonstrated that changes both in SMP and, especially, codend designs have a significant effect on hake and blue whiting size selectivity and catch patterns. Therefore, we believe that further research should prioritize codend size selectivity, and additional selection devices may be added once codend designs with good selective properties are achieved.

Molecular Characterization of Fungi Associated with Smoked Micromesistius poutassou (Blue whiting) from Different Markets in Lagos, Nigeria.

Micromesistius poutassou (Blue whiting) popularly called ‘’Panla’’ in Nigeria. It is one of the affordable fishes imported to Nigeria. With the rising cost of meat, milk and protein foods, consumers have become increasingly interested in fish as a source of protein for its availability and affordability rate. Smoked samples of Micromesistius poutassou were randomly purchased from ten different markets in ten local governments in Lagos state. This collection (purchase) were carried out between June and November 2019 (6 months). The associated fungi were isolated using the pour-plate method and extracted its DNA with the aid of Zymo DNA extraction kit. The extracted fungal DNA were amplified by PCR (Polymerase chain reaction) using specific internal transcribed spacer primer (ITS1/ITS4) and sequenced. Of the total 480 smoked Micromesistius poutassou sampled, ten fungi species were identified and characterised. These fungi include: Kodamaea ohmeri, Daldinia eschscholtzii, Aspergillus longivesica, Aspergillus flavus, Phoma sp, Aspergillus sp, Mucor circinelloides, Aspergillus tamarii, Aspergillus niger, and Geotrichum candidum. Results from this study revealed that smoked Micromesistius poutassou, which are being consumed by Lagosians on the daily basis were heavily contaminated with pathogenic fungi.

Intestinal parasites associated with common marine fishes in Nigeria

Objective: Fish is a valuable food source and employment in developing countries. However, par¬asitic infections pose a threat to fish cultivation and fish health. This study is aimed at assessing intestinal parasites associated with marine fishes. Materials and Methods: A total of 30 frozen marine fishes, comprising 10 Clupea harengus (her¬rings), 10 Scomber scombrus (mackerel), and 10 Micromesistius poutassou (blue whiting) of dif¬ferent sizes and weights, were examined for intestinal parasites. Results: The results revealed Eimeria spp. and Goussia spp. as parasitic protozoa in M. poutassou and S. scombrus, respectively. Grillotia smarisgora was the only parasitic cestode in M. poutas¬sou. At the same time, Hemiurus spp. and Anisakis spp. were parasitic trematode and nematode observed in both C. harengus and S. scombrus fishes, respectively. Anisakis spp., a parasitic nema¬tode, was the most common intestinal parasite among the marine fishes with 70%, 80%, and 90% occurrence in S. scombrus, C. harengus, and M. poutassou, respectively. Conclusion: Parasitic infection is one of the factors undermining fish production. Therefore, fish sellers should be enlightened to improve sanitation in handling and processing fish.