Soil Microcosms Research Articles

Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms.

Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of this contaminant in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms (Eisenia fetida) in natural soil matrix. PHE exhibited a relatively high toxicity to E. fetida in natural soil, with the LC50 determined to be 56.68mgkg-1 after a 14-day exposure. Excessive ROS induced by PHE, leading to oxidative damage to biomacromolecules in E. fetida, including lipid peroxidation, protein carbonylation, and DNA damage. The antioxidant defense system (total antioxidant capacity, glutathione S-transferase, peroxidase, catalase, carboxylesterase, and superoxide dismutase) in E. fetida responded quickly to scavenge excess ROS and free radicals. Exposure to PHE resulted in earthworm avoidance responses (2.5mgkg-1) and habitat function loss (10mgkg-1). Histological observations indicated that the intestine, body wall, and seminal vesicle in E. fetida were severely damaged after exposure to high-dose PHE. Moreover, earthworm growth (weight change) and reproduction (cocoon production and the number of juvenile) were also inhibited after exposure to this pollutant. Furthermore, the integrated toxicity of PHE toward E. fetida at different doses and exposure times was assessed by the integrated biomarker response (IBR), which confirmed that PHE is more toxic to earthworms in the high-dose and long-term exposure groups. Our results showed that PHE exposure induced oxidative stress, disturbed antioxidant defense system, and caused oxidative damage in E. fetida. These effects can trigger behavior changes and damage histological structure, finally cause growth inhibition, genotoxicity, and reproductive toxicity in earthworms. The strength of this study is the comprehensive toxicity evaluation of PHE to earthworms and highlights the need to investigate the eco-toxicity potential of exogenous environmental pollutants in a real soil environment.

Effect of CeO2 nanoparticles on plant growth and soil microcosm in a soil-plant interactive system

The impact of CeO2 nanoparticles (NPs) on plant physiology and soil microcosm and the underlying mechanism remains unclear to date. This study investigates the effect of CeO2 NPs on plant growth and soil microbial communities in both the rhizosphere of cucumber seedlings and the surrounding bulk soil, with CeCl3 as a comparison to identify the contribution of the particulate and ionic form to the phytotoxicity of CeO2 NPs. The results show that Ce was significantly accumulated in the cucumber tissue after CeO2 NPs exposure. In the roots, 5.3% of the accumulated Ce has transformed to Ce3+. This transformation might take place prior to uptake by the roots since 2.5% of CeO2 NPs was found transformed in the rhizosphere soil. However, the transformation of CeO2 NPs in the bulk soil was negligible, indicating the critical role of rhizosphere chemistry in the transformation. CeO2 NPs treatment induced oxidative stress in the roots, but the biomass of the roots was significantly increased, although the Vitamin C (Vc) content and soluble sugar content were decreased and mineral nutrient contents were altered. The soil enzymatic activity and the microbial community in both rhizosphere and bulk soil samples were altered, with rhizosphere soil showing more prominent changes. CeCl3 treatment induced similar effects although less than CeO2 NPs, suggesting that Ce3+ released from CeO2 NPs contributed to the CeO2 NPs induced impacts on soil health and plant physiology.

Performance analysis of Pseudomonas sp. strain SA3 in naphthalene degradation using phytotoxicity and microcosm studies.

The present study is aimed to develop a microbial system for efficient naphthalene bioremediation. A phytotoxicity study was carried out to check the naphthalene detoxification efficiency of Pseudomonas sp. strain SA3 in mung bean (Vigna radiata). For this, administration of the degraded product (supernatant) of 500 mg L-1 naphthalene by Pseudomonas sp. strain SA3 was studied on V. radiata till 168 h. The growth parameters of mung bean seedlings exposed to treated naphthalene solution were statistically similar to distilled water but a twofold decrease when exposed to untreated naphthalene solution. Further, through the soil microcosm study, the naphthalene degradation by pure colonies of Pseudomonas sp. strain SA3 was 6.8% higher as compared to when the natural microflora was mixed with Pseudomonas sp. strain SA3. Further naphthalene degradation by a microcosm model revealed that with an increased concentration of glucose, the carbon dioxide trap rate decreases.

Structural Selectivity of PAH Removal Processes in Soil, and the Effect of Metal Co-Contaminants

Polycyclic aromatic hydrocarbons (PAHs) form a convenient structural series of molecules with which to examine the selectivity exerted on their removal by soil microbiota. It is known that there is an inverse relationship between PAH molecular size and degradation rates in soil. In this paper, we look at how the magnitude of the slope for this relationship, m, can be used as an indicator of the effect of metal co-contaminants on degradation rates across a range of PAH molecular weights. The analysis utilises data collected from our previous microcosm study (Deary, M.E.; Ekumankama, C.C.; Cummings, S.P. Development of a novel kinetic model for the analysis of PAH biodegradation in the presence of lead and cadmium co-contaminants. Journal of Hazard Materials 2016, 307, 240–252) in which we followed the degradation of the 16 US EPA PAHs over 40 weeks in soil microcosms taken from a high organic matter content woodland soil. The soil was amended with a PAH mixture (total concentration of 2166 mg kg−1) and with a range of metal co-contaminant concentrations (lead, up to 782 mg kg−1; cadmium up to 620 mg kg−1; and mercury up to 1150 mg kg−1). It was found that the magnitude of m increases in relation to the applied concentration of metal co-contaminant, indicating a more adverse effect on microbial communities that participate in the removal of higher molecular weight PAHs. We conclude that m is a useful parameter by which we might measure the differential effects of environmental contaminants on the PAH removal. Such information will be useful in planning and implementing remediation strategies.

Short-term exposure to soils and sludge induce changes to plastic morphology and 13C stable isotopic composition

There is concern about the buildup of plastic waste in soil, their degradation into microplastics, and their potential to interfere with the natural processing of soil organic carbon and other nutrient cycling processes. Here we used scanning electron microscopy (SEM) and 13C isotope ratio mass spectrometry to determine if precut consumer plastics comprised of either high density polyethylene (HDPE), a blend of linear low density polyethylene and low density polyethylene (L/LDPE), or polyethylene terephthalate (PETE) would degrade or transform during a short-term, 32 day, exposure to soil or sludge in laboratory microcosms. SEM confirmed morphological changes occurred to all plastics, but the attachment of biofilm and presence of microorganisms mostly favored PETE and HDPE surfaces. These observations support the idea that abiotic and/or biotic processes may degrade plastics in soil; however distinguishable and significant changes in mean stable isotopic values (Δδ13C) of ~0.2–0.7‰ were only observed for exposed PETE and HDPE. This indicates that each plastic's degradation in soil may be dependent on their physical and chemical properties, with L/LDPE being more resistant and less prone to degradation compared to the others, and less dependent on the environmental conditions or properties of the soil or sludge. Our experiments were short-term and while the mechanisms of degradation are not clear, the results provide strong motivation for further studies of plastic fate and processing in soil systems. Direct mechanistic studies using stable isotopic approaches in combination with other characterizations and techniques are clearly warranted and may lead to a significant enhancement in our present understanding of the interactions and dynamics of plastics in the soil environment.

Protistan consumers and phototrophs are more sensitive than bacteria and fungi to pyrene exposure in soil

The levels of organic pollutants, in particular polycyclic aromatic hydrocarbons (PAHs), are increasing worldwide, yet we lack clarity on how these pollutants affect microbial communities of different trophic levels, including protists, fungi, and bacteria. Herein, we conducted soil microcosm incubation experiments to investigate the effects of pyrene, a typical PAH, on microbial communities along concentration gradients from 0 to 500 mg kg−1 soil. Protistan communities were more sensitive to pollutants than fungal and bacterial communities, and protistan consumers and phototrophs were the dominant trophic functional groups. In addition, by assessing changes in the diversity and structure of the soil microbiome and ecological networks, we found that the microbial communities, including the protistan community and the two trophic communities composed of protists and their prey, were destabilized with increasing stress and pyrene concentrations. We identified links and complicated relationships between phototrophs, bacteria, and consumers in food webs, which explain the importance of protists in stabilizing the microbial community. Collectively, our work provides novel evidence that protists are considerably sensitive to pollution stress, and caution should be exercised in future evaluations of the protistan and multitrophic communities in polluted soil ecosystems.

The individual and combined effects of polystyrene and silver nanoparticles on nitrogen transformation and bacterial communities in an agricultural soil

The effects of emerging contaminants micro/nanoplastics (MPs/NPs) and silver nanoparticles (Ag NPs) on health have attracted universal concern throughout the world. However, it is unclear on the combined effects of MPs/NPs and Ag NPs on the biogeochemistry cycle such as nitrogen transformation and functional microorganism in the soil. In the present study, we conducted a 45-day soil microcosm experiment with polystyrene (PS) MPs/NPs and Ag NPs to investigate their combined impact on nitrogen cycling and the bacterial community. The results showed that MPs or NPs exerted limited effects on nitrogen transformation in the soil. The combined effects of PS MPs/NPs and Ag NPs were mainly caused by the presence of Ag NPs. However, PS NPs alleviated the inhibition of anammox and denitrification induced by Ag NPs via upregulating anammox-related genes and elevating nitrate and nitrite reductase activities. PS MPs + Ag NPs treatment significantly reduced bacterial diversity. PS MPs/NPs + Ag NPs increased the relative abundances of denitrifying Cupriavidus by 0.32% and 0.06% but decreased nitrogen-fixing functional microorganisms of Microvirga (by 2.05% and 2.24%), Bacillus (by 0.16% and 0.22%), and Herbaspirillum (by 0.14% and 0.07%) at the genus level compared with Ag NPs alone. The significant downregulation of nitrogen-fixing genes (K02586, K02588, and K02591) was observed in PS MPs/NPs + Ag NPs treatment compared to Ag NPs in the nitrogen metabolism pathway. Moreover, g-Lysobacter and g-Aquimonas were identified as biomarkers in PS MPs + Ag NPs and PS NPs + Ag NPs by LEfSe analysis. Our study sheds the light that changes of functional microorganism abundances contributed to the alteration of nitrogen transformation. Taking the particle size of plastics into account will be helpful to accurately assess the combined ecological risks of plastics and nanomaterial contaminants.

Label-Free Multiphoton Imaging of Microbes in Root, Mineral, and Soil Matrices with Time-Gated Coherent Raman and Fluorescence Lifetime Imaging.

Imaging biogeochemical interactions in complex microbial systems─such as those at the soil-root interface─is crucial to studies of climate, agriculture, and environmental health but complicated by the three-dimensional (3D) juxtaposition of materials with a wide range of optical properties. We developed a label-free multiphoton nonlinear imaging approach to provide contrast and chemical information for soil microorganisms in roots and minerals with epi-illumination by simultaneously imaging two-photon excitation fluorescence (TPEF), coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and sum-frequency mixing (SFM). We used fluorescence lifetime imaging (FLIM) and time gating to correct CARS for the autofluorescence background native to soil particles and fungal hyphae (TG-CARS) using time-correlated single-photon counting (TCSPC). We combined TPEF, TG-CARS, and FLIM to maximize image contrast for live fungi and bacteria in roots and soil matrices without fluorescence labeling. Using this instrument, we imaged symbiotic arbuscular mycorrhizal fungi (AMF) structures within unstained plant roots in 3D to 60 μm depth. High-quality imaging was possible at up to 30 μm depth in a clay particle matrix and at 15 μm in complex soil preparation. TG-CARS allowed us to identify previously unknown lipid droplets in the symbiotic fungus, Serendipita bescii. We also visualized unstained putative bacteria associated with the roots of Brachypodium distachyon in a soil microcosm. Our results show that this multimodal approach holds significant promise for rhizosphere and soil science research.

Draft Genome Sequence of Leifsonia poae Strain BS71, Isolated from a Drought Microcosm.

ABSTRACTWe report the draft genome sequence of Leifsonia poae strain BS71. This bacterium was isolated from a low soil moisture content model soil microcosm inoculated with forest soil that had been subject to chronic warming.

Changes in Bacterial and Fungal Community of Soil under Treatment of Pesticides

Experiments were carried out in soil microcosms with the treatment of pesticide formulations—imidacloprid, benomyl, and metribuzin in single and tenfold application rates. For additional stimulation of microorganisms, a starch–mineral mixture was added to some variants. For all samples, high-throughput sequencing on the Illumina MiSeq platform of the V4 (16S rRNA) and ITS1 (18S rRNA) fragments was carried out. As a result, it was possible to establish the characteristic changes in the structure of the soil fungal and bacterial communities under pesticides application. The application of pesticides was accompanied by dramatic shifts in alfa-diversity of the fungal community. The phylum Basidiomycota was likely to be involved in the degradation of pesticides. The changes in the relative abundance of the genera Terrabacter, Kitasatospora, Streptomyces, Sphingomonas, Apiotrichum, Solicoccozyma, Gamsia, and Humicola can be proposed as an indicator of pesticide contamination. It is suggested to use these markers for large-scale assessment of the effect of pesticides on soil microbial communities instead of classical integral methods, including within the framework of state registration of pesticides. It is also recommended to research the effect of pesticides on the soil microbiome during artificially initiated successions using the additional source of carbon.

Microbial Depolymerization of Epoxy Resins: A Novel Approach to a Complex Challenge

The objective of this project is evaluating the potential of microbes (fungi and bacteria) for the depolymerization of epoxy, aiming at the development of a circular management of natural resources for epoxy in a long-term prospective. For depolymerization, epoxy samples were incubated for 1, 3, 6 and 9 months in soil microcosms inoculated with Ganoderma adspersum. Contact angle data revealed a reduction in the hydrophobicity induced by the fungus. Environmental scanning electron microscopy on epoxy samples incubated for more than 3 years in microbiological water revealed abundant microbiota. This comprised microbes of different sizes and shapes. The fungi Trichoderma harzianum and Aspergillus calidoustus, as well as the bacteria Variovorax sp. and Methyloversatilis discipulorum, were isolated from this environment. Altogether, these results suggest that microbes are able to colonize epoxy surfaces and, most probably, also partially depolymerize them. This could open promising opportunities for the study of new metabolisms potentially able depolymerize epoxy materials.

Polyamide Microplastic Alters Microbial Community and Carbon and Nitrogen Cycles in a Simulated Agricultural Soil Microcosm

Polyamide Microplastic Alters Microbial Community and Carbon and Nitrogen Cycles in a Simulated Agricultural Soil Microcosm

Copper speciation and mobility in glyphosate co-contaminated soils: a microcosm and X-ray absorption spectroscopy study

Context The effect of glyphosate–copper complex on copper mobility had been studied in aqueous conditions. The effect on copper distribution through the soil profile is little understood and controversial. Aims This study aims to investigate the effect of glyphosate on copper mobility and distribution through the soil profile and to identify the specific copper binding change at the molecular level. Methods The experiments were performed in microcosm soil spiked with copper and glyphosate. Sequential extraction and X-ray absorption spectroscopy techniques were applied for copper speciation. Key results The organic bound fraction was the highest fraction (50%) in the top soil layer, while Cu(CH3COO)2 (43%) and Cu(NO3)2 (49%) were the highest fraction in linear combination fit (LCF) analysis. In the presence of glyphosate, the readily mobile fraction of copper (water soluble and exchangeable fractions) increased, while the mobilised fractions decreased within 1 day of glyphosate application. The LCF analysis revealed that CuSO4 and Cu3(PO4)2 increased in the presence of glyphosate. The effects of glyphosate were no longer detected by 14 days after application. During the 40 days of the experiment, the released copper did not leach beyond 4 cm deep in soil. Conclusions The existence of glyphosate in soil induced the release of copper into the readily mobilised fraction within 1 day of glyphosate contamination. The effect was found within 4 cm depth and declined in correspondence with the degradation half-life of glyphosate. Implications The finding is important in evaluating the risk of metal diffusion and nutrient leaching from co-contaminated soil through the soil profile over time and at a molecular scale.

Predicting the occurrence of monooxygenases and their associated phylotypes in soil microcosms

Cometabolic oxidation involves the oxidation of chemicals often by monooxygenases or dioxygenases and can be a removal process for environmental contaminants such as trichloroethene (TCE) or 1,4-dioxane. Information on the occurrence of these genes and their associated microorganisms in environmental samples has the potential to enhance our understanding of contaminant removal. The overall aims were to 1) ascertain which genes encoding for monooxygenases (from methanotrophs, ammonia oxidizing bacteria and toluene/phenol oxidizers) and other key enzymes are present in soil microcosms and 2) determine which phylotypes are associated with those genes.The approach involved a predictive tool called PICRUSt2 and 16S rRNA gene amplicon datasets from two previous soil microcosm studies. The following targets from the KEGG database were examined: pmo/amo, mmo, dmp/pox/tomA, tmo/tbu/tou, bssABC (and downstream genes), tod, xylM, xylA, gst, dhaA, catE, dbfA1, dbfA2 and phenol 2-monooxygenase.A large number of phylotypes were associated with pmo/amo, while mmo was linked to only five. Several phylotypes were associated with both pmo/amo and mmo. The most dominant microorganism predicted for mmoX was Mycobacterium (also predicted for pmo/amo). A large number of phylotypes were associated with all six genes from the dmp/pox/tomA KEGG group. The taxonomic associations predicted for the tmo/tbu/tou KEGG group were more limited. In both datasets, Geobacter was a key phylotype for benzylsuccinate synthase. The dioxygenase-mediated toluene degradation pathway encoded by todC1C2BA was largely absent, as were the genes (xylM, xylA) encoding for xylene monooxygenase. All other genes investigated were predicted to be present and were associated with a number of microorganisms. Overall, the analysis predicted the genes encoding for sMMO (mmo), T3MO/T3MO/ToMO (tmo/tbu/tou) and benzylsuccinate synthase (bssABC) are present for a limited number of phylotypes compared to those encoding for pMMO/AMO (pmo/amo) and phenol monooxygenase/T2MO (dmp/poxA/tomA). These findings suggest in soils contaminant removal via pMMO/AMO or phenol monooxygenase/T2MO may be common because of the occurrence of these enzymes with a large number of phylotypes.

Bioaugmentation of Enterobacter cloacae AKS7 causes an enhanced degradation of low-density polyethylene (LDPE) in soil: a promising approach for the sustainable management of LDPE waste.

Enterobacter cloacae AKS7 was previously reported to degrade UV-treated low-density polyethylene (LDPE) more efficiently than UV-untreated LDPE. However, the degradation of LDPE by Enterobacter cloacae AKS7 at the LDPE-contaminated soil remained unaddressed. To address this issue, soil microcosms were prepared in which an equal amount of either UV-treated or UV-untreated LDPE was added. Then, the microcosms were either augmented with AKS7 or left non-augmented. We observed that the bioaugmented microcosms exhibited approximately twofold greater polymer degradation than non-bioaugmented microcosms. To investigate the underlying cause, we found that the abundance of LDPE-degrading organisms got increased by approximately fivefold in bioaugmented microcosms than non-bioaugmented microcosms. The microbial biomass carbon and nitrogen content got enhanced by approximately twofold in bioaugmented microcosms as contrasted to non-bioaugmented microcosms. Furthermore, the bioaugmented microcosms showed almost twofold increase in the level of dehydrogenase and fluorescein diacetate (FDA) hydrolyzing activity than the non-bioaugmented microcosms. To add on, Shannon-diversity index and Gini coefficient were determined in each microcosm to measure the microbial richness and evenness, respectively, using the results of carbon source utilization pattern of BiOLOG ECO plate. The bioaugmented microcosms exhibited ~ 30% higher functional richness and ~ 30% enhanced functional evenness than the non-bioaugmented microcosms indicating the formation of an enriched ecosystem that could offer various functions including polymer degradation. Taken together, the results suggested that Enterobacter cloacae AKS7 could be used as a promising bioaugmenting agent for the sustainable degradation of LDPE waste at a contaminated site.

Biotransformation of dimethoate into novel metabolites by bacterial isolate Pseudomonas kilonensis MB490

Bacterial strain (Pseudomonas kilonensis MB490) isolated from agricultural fields of Mianwali, was selected to check its potential to degrade Organophosphate insecticide dimethoate (DM). Strain MB490 was able to degrade dimethoate equally well at given pH range (6.0, 7.0 and 8.0), thus showing its pH independence for dimethoate degradation. Optimum temperature for dimethoate degradation varied from 25–30 °C. There was more dimethoate degradation under shaking conditions with optimum growth. Strain MB490 showed 90% dimethoate degradation in M-9 broth and 90.6% in soil slurry, while exhibited 81.5% dimethoate degradation in soil microcosm within 9 days, based on HPLC analysis of bacterial samples supplemented with 200 mg/L dimethoate. The average half-life (t 1/2) of dimethoate after bacterial degradation ranged from 1.95 days in 1st phase to 5 days in 2nd phase in M-9 broth, soil slurry and soil microcosm, while in control media without bacteria, it ranged from 30 to 64.3 days. GCMS investigation revealed the transformation of dimethoate into 5 metabolic products namely Methyl diethanol amine, Aspartylglycine ethyl ester, Phosphonothioic acid propyl-O, S-dimethyl ester, O, O, O-Trimethyl thiophosphate and omethoate which were ultimately mineralized by the strain MB490, providing energy for its growth.

Anaerobic methane oxidation linked to Fe(III) reduction in a Candidatus Methanoperedens-enriched consortium from the cold Zoige wetland at Tibetan Plateau.

Anaerobic oxidation of methane (AOM) is a microbial process degrading ample methane in anoxic environments, and Ca. Methanoperedens mediated nitrate- or metal-reduction linked AOM is believed important in freshwater systems. This work, via 16S rRNA gene diversity survey and 16S rRNA quantification, found abundant Ca. Methanoperedens along with iron in the cold Zoige wetland at Tibetan Plateau. The wetland soil microcosm performed Fe(III) reduction, rather than nitrate- nor sulphate-reduction, coupled methane oxidation (3.87 μmol d-1 ) with 32.33 μmol Fe(II) accumulation per day at 18°C, but not at 30°C. A metagenome-assembled genome (MAG) recovered from the microcosm exhibits ~74% average nucleotide identity with the reported Ca. Methanoperedens spp. that perform Fe(III) reduction linked AOM, thus a novel species Ca. Methanoperedens psychrophilus was proposed. Ca. M. psychrophilus contains the whole suite of CO2 reductive methanogenic genes presumably involving in AOM via a reverse direction, and comparative genome analysis revealed its unique gene categories: the multi-heme clusters (MHCs) cytochromes, the S-layer proteins highly homologous to those recovered from lower temperature environments and type IV pili, those could confer Ca. M. psychrophilus of cold adaptability. Therefore, this work reports the first methanotroph implementing AOM in an alpine wetland.

Towards Sustainable Dairy Production in Argentina: Evaluating Nutrient and CO2 Release from Raw and Processed Farm Waste

Mineralization studies are the first step in determining the usefulness of an amendment such as fertilizer, and are essential to creating guidelines for dairy waste management to help producers make informed decisions. Our goal was to assess the effects of dairy raw, composted, and digested manure amendments on C, N, and P mineralization to evaluate the feasibility of their in-farm production and use as organic fertilizers. The liquid and solid fractions of dairy effluent (LDE, SDE), dairy effluent digestate (DED), onion–cattle manure digestate and compost (OCMD, OCMC) were characterized by chemical and spectroscopic methods. Soil microcosms with LDE, SDE, DED, OCMD and OCMC and the C, N and P mineralization were determined periodically. Elemental and structural differences among amendments led to contrasting profiles of C, N, and P mineralization, and thus to differences in nutrient availability, immobilization, and CO2 emission. All processed materials were more stable than untreated waste, reducing C emissions. Digestates showed net C immobilization, and supplied the highest levels of available N, creating a relative P deficit. Instead, the compost supplied N and P via mineralization, producing a relative P excess. Future studies should aim at evaluating fertilization strategies that combine both kinds of amendments, to exploit their complimentary agronomic characteristics.

Microbial communities on biodegradable plastics under different fertilization practices in farmland soil microcosms

Plastic mulching is a common practice in agricultural systems and is often combined with fertilization. Biodegradable plastics (BPs) are becoming an alternative to non-biodegradable plastics (non-BPs) for soil mulching. However, the effects of fertilization on the microbial communities on BPs remain unclear. Here, we explored the responses of the plastisphere to different fertilization practices in soil-based microcosms containing three BPs: polylactic acid (PLA), poly (butylene succinate) (PBS), and poly (butylene-adipate-co-terephthalate) (PBAT), and one non-BP (low-density polyethylene, LDPE). The 16S and ITS rRNA gene-based Illumina sequencing method were used to identify the bacterial and fungal communities on the plastics and in the soils. Microbial community structure on BPs was significantly different from that in soils and on LDPE. The predicted functional profiles of bacteria on BPs, especially PBAT, were distinct from those in soils. The plastisphere communities on BPs were dominated by microbes adapted to access and utilize carbon sources compared with of the communities on LDPE. Application of manure increased the alpha diversity of bacterial communities on BPs but decreased it on LDPE. The structure of bacterial communities on BPs changed with the application of manure. Our research establishes the baseline dynamics of plastisphere communities on BPs in soils.

Synergy between fungi and bacteria promotes polycyclic aromatic hydrocarbon cometabolism in lignin-amended soil

Lignin enhanced biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soil, but collaboration among soil microorganisms during this process remains poorly understood. Here we explored the relations between microbial communities and PAH transformation in soil microcosms amended with lignin. Mineralization of the four-ring benzo(a)anthracene (BaA), which was selected as a model, was determined by using an isotope-labeled tracer. The eukaryotic inhibitor cycloheximide and redox mediator ABTS were used to validate the fungal role, while microbial communities were monitored by amplicon sequencing. The results demonstrated that lignin significantly promoted BaA mineralization to CO2, which was inhibited and enhanced by cycloheximide and ABTS, respectively. Together with the increased abundance of Basidiomycota, these observations suggested an essential contribution of fungi to BaA biodegradation, which possibly through a ligninolytic enzyme-mediated pathway. The enrichment of Methylophilaceae and Sphingomonadaceae supported bacterial utilization of methyl and aryl groups derived from lignin, implicating cometabolic BaA degradation. Co-occurrence network analysis revealed increased interactions between fungi and bacteria, suggesting they played synergistic roles in the transformation of lignin and BaA. Collectively, these findings demonstrate the importance of synergy between fungi and bacteria in PAH transformation, and further suggest that the modulation of microbial interplay may ameliorate soil bioremediation with natural materials such as lignin.