Microbially Induced Calcium Carbonate Precipitation Research Articles

Experimental Study on the Solidification of Uranium Tailings and Uranium Removal Based on MICP

The governance of uranium tailings aims to improve stability and reduce radionuclide uranium release. In order to achieve this goal, the uranium removal solution test and uranium tailings grouting test were successively carried out using microbially induced calcium carbonate precipitation (MICP) technology. The effect of MICP on the reinforcement of uranium tailings and the synchronous control of radionuclide uranium in the tailings were discussed. The solution test results show that Sporosarcina pasteurii could grow and reproduce rapidly in an acidic medium with an initial pH of 5. The uranium concentration decreased with the increase in MICP reaction time, and the removal efficiency reached 60.9% at 24 h. In the solidification test of tailings, the strength of tailings improved significantly after 12 days of reinforcement, with an increase in the cohesion of tailings by 2.937 times and an increased internal friction angle of 8.393°. The peak stress value of solidified tailings at the surrounding pressure of 50 kPa increased by 1.87 times, and the uranium concentration in the discharge fluid decreased by 76.91% compared to the blank group. This study provides valuable insights and references for safely disposing of uranium tailings.

Removal of cadmium and arsenic from water through biomineralization.

Due to anthropogenic activities, heavy metals such as cadmium (Cd) and arsenic (As) are one of the most toxic xenobiotics contaminating water, thus affecting human health and the environment. The objective of the present investigation was to study the effect of ureolytic bacteria Bacillus paramycoides-MSR1 for the bioremediation of Cd and As from contaminated water. The B. paramycoides showed high resistance to heavy metals, Cd and As, with minimum inhibitory concentration (MIC) of 12.84 μM and 48.54 μM, respectively. The urease activity and calcium carbonate (CaCO3) precipitation were evaluated in artificial wastewater with different concentrations of Cd (0, 10, 20, 30, 40, 50, and 60 μM) and As (0, 20, 40, 60, 80, and 100 μM). The maximum urease activity in Cd-contaminated artificial wastewater was observed after 96 hours, which showed a 76.1% decline in urease activity as the metal concentration increased from 0 to 60 μM. Similarly, 14.1% decline in urease activity was observed as the concentration of As was increased from 0 to 100 μM. The calcium carbonate precipitation at the minimum inhibitory concentration of Cd and As-contaminated artificial wastewater was 189 and 183 mg/100 ml, respectively. The percentage removal of metal from artificially contaminated wastewater with varied concentrations was analyzed using atomic absorption spectroscopy (AAS). After 168 hours of incubation, 93.13% removal of Cd and 94.25% removal of As were observed. Microstructural analysis proved the presence of calcium carbonate in the form of calcite, confirming removal of cadmium and arsenic by microbially induced calcium carbonate precipitation (MICCP) to be promising technique for water decontamination.

Performance and Mechanism of Zn-Contaminated Soil through Microbe-Induced Calcium Carbonate Precipitation

Zn is a toxic heavy metal that seriously endangers human health and ecological stability. For a long time, traditional remediation techniques have been used to remediate Zn-contaminated soil prone to other problems such as secondary contamination. In recent years, due to the great danger posed by Zn pollution, there has been an increasing interest in applying eco-friendly and sustainable methods to remediate Zn-contaminated soil. Therefore, in this study, microbially induced calcium carbonate precipitation (MICP) technology was used to bioremediate zinc ions by transforming ionic heavy metals into insoluble solid-phase minerals. Through the unconfined compressive strength (UCS) test, direct shear (DS) test, and penetration test (PT), the results showed that the unconfined compressive strength of the treated specimens increased by 187.2~550.5%, the cohesion increased significantly compared with the internal friction angle of specimens, and the permeability coefficient can be reduced by at least one order of magnitude. During the treatment of Zn pollutants, the mobility of heavy metal zinc ions was significantly reduced, the percentage of exchangeable state Zn content was significantly reduced, and the leaching concentration of zinc ions in Zn-contaminated soil was reduced to about 20 mg/L, which was significantly lower than the limit in the standard (100 mg/L). These results were further confirmed by scanning electron microscope (SEM) and X-ray diffraction (XRD) analyses, which indicated coprecipitation of calcium carbonate (CaCO3) and ZnCO3. The microbial solidification/stabilization of Zn-contaminated soil was most effective when the curing age of 28 d, the cementation solution concentration of 1 mol/L, and the cementation solution ratio of 1:2. Therefore, the bio-immobilization of zinc ions by MICP has the potential for application as a low-cost and eco-friendly method for heavy metal remediation.

Coupling reinforcement of uranium tailings via Klebsiella-induced calcium carbonate precipitation and waterborne polyurethane

The environmentally friendly and efficient realization of uranium tailings dam reinforcement is of great significance for the sustainable development of the uranium mining industry. Herein, the effects of microbially induced calcium carbonate precipitation (MICP), using Klebsiella, and waterborne polyurethane (WPU) coupled reinforcement on the mechanical behavior of uranium tailings were studied by triaxial testing. It was demonstrated that the mechanical behaviors of the specimens treated with MICP-WPU coupled reinforcement had a more significant improvement than those treated with MICP alone. When the test confining pressure was 400 kPa, it was found that the peak deviatoric stress of the specimens treated with the coupled reinforcement increased by 45.7 %, the elastic modulus increased by 231.3 %, and the cohesive force was enhanced to 217 kPa as compared to the specimen reinforcement of only MICP induced by Klebsiella. The coupled reinforcement mechanism study using XRD and SEM shows that the significant improvement in the mechanical behavior of the specimens is attributed to the spatial reticular structure formed by wrapping and binding vaterite crystals and tailings particles with WPU. This innovative green reinforcement method is expected to be a new alternative for stabilizing unstable soils in mine geotechnical engineering.

Experimental Study on the Effect of an Organic Matrix on Improving the Strength of Tailings Strengthened by MICP.

In order to improve the effect of microbial-induced calcium carbonate precipitation (MICP) in tailings reinforcement, sodium citrate, an organic matrix with good water solubility, was selected as the crystal form adjustment template for inducing calcium carbonate crystallization, and the reinforcements of tailings by MICP were conducted in several experiments. The effects of sodium citrate on the yield, crystal form, crystal appearance, and distribution of calcium carbonate were analyzed by MICP solution test; thus, the related results were obtained. These showed that the addition of a proper amount of organic matrix sodium citrate could result in an increment in the yield of calcium carbonate. The growth rate of calcium carbonate reached 22.6% under the optimum amount of sodium citrate, and the crystals of calcium carbonate were diverse and closely arranged. Based on this, the MICP reinforcement test of tailings was carried out under the action of the optimum amount of sodium citrate. The microscopic analysis using CT and other means showed that the calcium carbonate is distributed more uniformly in tailings, and the porosity of samples is significantly reduced by layered scanning analysis. The results of triaxial shear tests showed that adding organic matrix sodium citrate effectively increased the cohesion, internal friction angle, and peak stress of the reinforced tailings. It aims to provide a novel idea, a creative approach, and a method to enhance the reinforcement effect of tailings and green solidification technology in the mining environment.

Steerable artificial magnetic bacteria with target delivery ability of calcium carbonate for soil improvement.

The microbial-induced carbonate precipitation (MICP) has acquired significant attention due to its immense potential in sustainable engineering applications, particularly in soil improvement. However, the precise control of microbial-induced calcium carbonate precipitation remains a formidable challenge in engineering practices, owing to the uncertain movement paths of bacteria and the nonuniform distribution of soil pores. Taking inspiration from targeted therapy in medicine, this paper presents novel research on the development and validation of magnetically responsive bacteria. These bacteria demonstrate the ability to target calcium carbonate precipitation in a microfluidic chip, thereby promoting an environmentally friendly and ecologically sustainable biomineralization paradigm. The study focuses on investigating the migration of magnetite nanoparticles (MNPs) in aqueous solutions and enhancing the stability of MNP culture liquids. A specially designed microfluidic chip is utilized to simulate natural sand particles and their pores, while an external magnetic field is applied to precisely control the movement path of the artificial magnetic bacteria, enabling targeted precipitation of calcium carbonate at the micron-scale. Verification of the engineered artificial magnetic bacteria and their ability to induce calcium carbonate precipitation is conducted through SEM-EDS analysis, microfluidic chip observations, and the application of the K-means algorithm and ImageJ software to analyze calcium carbonate formation. The influence of the concentration of magnetic nanoparticles on the calcium carbonate production rate was also studied. The results confirm the potential of the artificial magnetic bacteria for future engineering applications. KEY POINTS: • Sporosarcina pasteurii is first time successfully engineered into artificial magnetic bacteria. • The artificial magnetic bacteria show excellent performance of targeted transportation and directional deposition of CaCO3 in microfluidic chip. • The emergence of artificial magnetic bacteria promotes paradigm shift of next generation environmentally friendly biomineralization.

An autonomous system to bio-bricks production by biomineralization activity

Urbanization causes a significant increase in the consumption of building materials, with negative environmental effects. Thus, it is a priority to develop alternative applications that promote infrastructure progress and reduction in the environmental impacts derived from civil construction activities. One example is microbial-induced calcium carbonate precipitation (MICP), a process by which ureolytic bacteria, such as Sporosarcina pasteurii, induce calcium carbonate (CaCO3) precipitation by urea hydrolysis. The shells are a by-product that has great potential for various uses and applications in industry. In the search for a more sustainable process, this study seeks to evaluate CaCO3 - shell powder as an aggregate to replace Portland cement with bio cement synthesized by S. pasteurii. Based on the autonomous mini-controlled irrigation system, was possible to maintain S. pasteurii activity that aggregates the particles via a biomineralization process.

Microbially Induced Calcium Carbonate Precipitation by Sporosarcina pasteurii: a Case Study in Optimizing Biological CaCO3 Precipitation.

Current production of traditional concrete requires enormous energy investment that accounts for approximately 5 to 8% of the world's annual CO2 production. Biocement is a building material that is already in industrial use and has the potential to rival traditional concrete as a more convenient and more environmentally friendly alternative. Biocement relies on biological structures (enzymes, cells, and/or cellular superstructures) to mineralize and bind particles in aggregate materials (e.g., sand and soil particles). Sporosarcina pasteurii is a workhorse organism for biocementation, but most research to date has focused on S. pasteurii as a building material rather than a biological system. In this review, we synthesize available materials science, microbiology, biochemistry, and cell biology evidence regarding biological CaCO3 precipitation and the role of microbes in microbially induced calcium carbonate precipitation (MICP) with a focus on S. pasteurii. Based on the available information, we provide a model that describes the molecular and cellular processes involved in converting feedstock material (urea and Ca2+) into cement. The model provides a foundational framework that we use to highlight particular targets for researchers as they proceed into optimizing the biology of MICP for biocement production.

Cementation anisotropy associated with microbially induced calcium-carbonate precipitation and its treatment effect on calcareous and quartz sands

Microbially induced calcium-carbonate precipitation (MICP) can help cement sand particles together and has emerged as a promising alternative to traditional ground-improvement treatments for granular soils. Owing to precipitation and absorption in different directions, MICP leads to anisotropic cementation. This study was aimed at examining the mechanism of cementation anisotropy and its treatment effect on two commonly used granular soils in geotechnical engineering: quartz sand and calcareous sand. A novel MICP technique was used to prepare bio-cemented sand specimens with different anisotropic patterns of cementation. Microscopic detection techniques were applied to examine the microstructure of the produced MICP cementation. The cementation in the vertical and horizontal directions was different, owing to gravity. The granular surface properties affected the content, morphology, and cement force of the bio-cements produced by MICP treatment. The results of unconfined compression strength experiments confirmed the occurrence of cementation anisotropy and its substantial effects on the stiffness, strength, and fracture patterns of MICP-treated quartz sand and calcareous sand. These findings can help to clarify the formation mechanism of bio-cements and promote the optimised application of MICP for the treatment of granular soils.

Mechanical Properties of Eolian Sand Solidified by Microbially Induced Calcium Carbonate Precipitation (MICP)

Eolian sand is widely distributed in desert areas, and can easily trigger sandstorms under the combined effects of wind and thermal conditions. In this paper, an eolian sand solidification test via microbially induced calcium carbonate (CaCO3) precipitation (MICP) was conducted to explore prospective applications of MICP in the field of dust prevention and sand fixation. A bacterial growth test and a MICP mineralization reaction test were performed based on the response surface method and an orthogonal experiment. Based on eolian sand solidification test, the effects of cementation number and dry density on the permeability and strength characteristics of solidified eolian sand, as well as the content of CaCO3 generated were analyzed. The results showed that the bacteria grew best at a temperature of 35 °C, a pH of 9, and a shaking frequency of 170 rpm. Both urease activity and bacterial concentration increased as the temperature and pH increased but showed a trend of rising before falling as the shaking grew faster. The optimal conditions for MICP mineralization were a bacterial concentration of OD600=1.5, a consolidating fluid concentration of 1 mol/L, a reaction time of 16 h, a pH of 9, and a temperature of 25 °C. The permeability coefficient of solidified eolian sand decreased as the dry density and the number of cementations increased, whereas the CaCO3 content increased with the number of cementations. As the number of cementations increased, the unconfined compressive strength (UCS) of eolian sand initially rose before stabilizing and then increasing again. The CaCO3 content had a positive relationship with UCS, which with the correlation coefficient reached 0.80.

Effect of incorporating discarded facial mask fiber on mechanical properties of MICP–treated sand

Responding to environmental challenges associated with the waste generated from discarded facial mask fiber (DFMF), this study introduces an innovative approach to integrate DFMF as a beneficial additive to Microbial-induced calcium carbonate precipitation (MICP) technology - an eco-friendly soil stabilization method. Despite its merit of enhancing soil strength and permeability, MICP often incurs substantial soil brittleness. The incorporation of DFMF, a material conventionally destined for landfill, demonstrates an enhancement in the ductility of MICP–treated sand. Various proportions of DFMF were incorporated (0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5% by weight), leading to an observable improvement in unconfined compressive strength (UCS), splitting tensile strength (STS) and in the microstructural configuration of MICP-treated sand. The presence of DFMF fostered bacterial immobilization, catalyzed CaCO3 production, and culminated in an optimum DFMF content at 0.2%. This not only mitigated soil brittleness by introducing ductility via calcite precipitation but also culminated in a “sand particles-fibers-sand particles” spatial configuration, reducing the weakening effect of water on MICP-treated sand samples. This study, therefore, highlights the potential of DFMF as a promising additive in MICP-treated sand, thereby offering an effective method for recycling and reusing discarded facial mask fibers.

A potential sustainable technique to entrap contaminants against rill erosion based on MICP

Health risks due to toxic metals (TM) contamination is a global concern. Toxic metals could be release to the environment due to soil erosion. The present study was aimed to prove the synergistic mechanisms of microbially induced calcium carbonate precipitation (MICP) on improving the strength properties of soil particles toward rill erosion, as well as immobilizing toxic metals into stable crystals. Hence, laboratory batch studies were conducted to evaluate the rill erosion stability as well as Cd, Pb and Zn removal ability based on bioprecipitation through MICP, using urease producing bacteria. Results revealed that the application of the strain Bacillus pasteurii PTCC1645 in MICP process of test model treated with 500 mM cementation solution showed a great potential to decrease sand lost to below 20% with the flow rate of 15 ml/min with highest strength (131 KPa) and the highest calcium carbonate content (0.84%). Moreover, the immobilization of the toxic metals in biotreated test models above 50 mM under the flow rate of 3 ml/min showed the efficiency of Pb, Zn and Cd ions by 100%. SEM proved the presence of calcite among the sand particles which could be attributed to the effectiveness of the strain. Energy-dispersive X-ray spectroscopy(EDS) analysis revealed the presence of toxic metals, while were transformed into carbonates by MICP of PTCC1645. Hence, toxic metals have stronger stability encountered with rill erosion and low toxicity compared with metal ions. The present study illustrated that the carbonate-biomineralization can offer an effective and eco-friendly approach to immobilize soluble toxic metals and that MICP may play an important role in metal bioconservation during rill erosion.

Growing bio-tiles using microbially induced calcium carbonate precipitation

Using the biomimetic process known as microbially induced calcium carbonate precipitation (MICP), the growth of bio-tiles was investigated as an alternative to conventionally fired ceramic tiles which require operating temperatures above 1000 °C, therefore adding to global carbon emissions. The ureolytic activity of Sporosarcina pasteurii was controlled by centrifuging and dilution with fresh yeast extract media. The bio-tiles were grown using a novel submersion method in which custom moulds were placed in exact positions within the bio-reactor and each was mixed individually from beneath. Five parameters were optimised to achieve bio-tiles (dimensions of 100 × 100 × 10 mm) of breaking strength comparable to conventional tiles of equivalent thickness. By optimising ureolytic activity (4.0 mmol/L·min), the cementation solution concentration (0.3 M), the particle size distribution (D10 = 312 μm; D50 = 469 μm), the volume of cementation solution, as well as the addition of supplemental magnesium (0.3 M), bio-tiles with a breaking strength 637 N ± 60 N and a modulus of rupture of 13.0 N/mm2 ± 2.3 N were produced. These parameters exceed the conventional standards of breaking strength and modulus of rupture of 600 N and 8 N/mm2, respectively, the standards set for tiles with a water absorption above 10 %. This is also the first time that an optimum CaCO3 precipitation rate constant has been identified (0.11–0.18 day−1) for producing bio-tiles that meet the strength properties of conventional extruded ceramic tiles. The tile manufacturing technique described in this study is easy to operate and scale since multiple bio-tiles can be produced in larger cementation tanks. This natural tile making process also benefits the environment by operating at room temperature.

Microbially induced calcium carbonate precipitation in fossil consolidation treatments: Preliminary results inducing exogenous Myxococcus xanthus bacteria in a miocene Cheirogaster richardi specimen

This research paper proposes Microbially Induced Calcium Carbonate Precipitation (MICP) as an innovative approach for palaeontological heritage conservation, specifically on deteriorated carbonate fossils. Due to its efficiency in bioconsolidation of carbonate ornamental rocks, Myxococcus xanthus inoculation on carbonate fossils was studied in this research.Treatment was tested on nine fossil samples from decontextualized fragments of Cheirogaster richardi specimens (Can Mata site, Hostalets de Pierola, Catalonia, Spain). The main objective was to evaluate whether treatment with Myxococcus xanthus improved fossil surface cohesion and hardness and mechanical strength without significant physicochemical and aesthetic changes to the surface. Chemical compatibility of the treatment, penetration capacity and absence of noticeable changes in substrate porosity were considered as important issues to be evaluated.Samples were analysed, before and after treatment, by scanning electron microscopy, weight control, spectrophotometry, X-ray diffraction analysis, water absorption analysis, pH and conductivity control, Vickers microindentation and tape test. Results show that hardness increases by a factor of almost two. Cohesion also increases and surface disaggregated particles are bonded together by a calcium carbonate micrometric layer with no noticeable changes in surface roughness. Colour and gloss variations are negligible, and pH, conductivity and weight hardly change. Slight changes in porosity were observed but without total pore clogging.To sum up, results indicate that Myxococcus xanthus biomineralisation is an effective consolidation treatment for carbonate fossils and highly compatible with carbonate substrates. Furthermore, bacterial precipitation of calcium carbonate is a safe and eco-friendly consolidation treatment.

Optimization of growth medium for microbially induced calcium carbonate precipitation (MICP) treatment of desert sand

Optimization of growth medium for microbially induced calcium carbonate precipitation (MICP) treatment of desert sand

Multi-scale analysis of the mechanism of microbially induced calcium carbonate precipitation consolidation loess.

Microbial-induced calcium carbonate precipitation (MICP) treatment of consolidated loess has the advantages of high efficiency and environmental protection. In this study, changes in the microscopic pore structure of loess before and after MICP treatment were compared and quantified, combined with test results at different scales, to better understand the mechanisms of MICP-consolidated loess. The unconfined compressive strength (UCS) of MICP-consolidated loess is significantly increased, and the stress-strain curve indicates improved strength and stability of the loess. X-ray diffraction (XRD) test results show that the signal strength of calcium carbonate crystals is significantly enhanced after loess consolidation. The microstructure of the loess was determined by scanning electron microscopy (SEM). The loess SEM microstructure images are quantitatively analyzed using comprehensive image processing methods (including gamma adjustment, grayscale threshold selection, median processing). The changes in microscopic pore area and average pore sizes (Feret diameter) of the loess before and after consolidation are described. More than 95% of the pores consist of pores with a pore area of less than 100 μm2 and an average pore size of less than 20μm. The total percentage of pore numbers with pore areas of 100-200 and 200-1000 μm2 decreased by 1.15% after MICP consolidation, while those with 0-1 and 1-100 μm2 increased. The percentage of pore numbers with an average pore size greater than 20μm decreased by 0.93%, while the 0-1, 1-10, and 10-20μm increased. Particle size distributions revealed a significant increase in particle size after MICP consolidation, with an increase of 89μm in D50.

Reduction of rainfall infiltration in soil slope using a controllable biocementation method

Reasonable control of rainwater infiltration rate can ensure that soil slope will not fail due to rapid infiltration of rainwater in heavy rainfall, and at the same time, more rainwater can be infiltrated in light rainfall to meet the water demand of animals and plants. In this study, based on microbial-induced calcium carbonate precipitation (MICP) technique, a controllable bio-method for rainfall infiltration of soil slope was proposed. To have a comprehensive understanding the relationship among the rainwater infiltration control capacity, biocement treated soil permeability, slope angle and rainfall intensity, a series of physical modelling experiments of rainfall diversion on slopes with three types of soils and three slope angles were carried out in the presence of various rainfall intensities. Experimental results indicated that the proposed bio-method had the ability of controlling rainwater infiltration in term of varying rounds of biocement spraying treatment. In general, it was found that the rainwater infiltration decreases with the increase in slope angle and rainfall intensity. In the worst case of smallest slope angle (15°) and lightest rainfall intensity (n = 50 mm/h), more than 82.6%, 92.2% and 84.4% of rainwater were prevented from infiltration into the MICP treated natural sand, fine sand and medium sand, respectively, while the untreated soils were not able to prevent the rainwater infiltration at all. The corresponding maximum local uniaxial compressive strength for the MICP treated natural sand, fine sand and medium sand, respectively, were found to be 2.3 MPa, 2.0 MPa, 2.6 MPa, whereas the flexural stresses were 0.46 MPa, 0.33 MPa, 0.67 MPa, which could resist rainfall droplet impact force. Overall, the proposed bio-method showed good rainwater infiltration control capacity and high bearing strength against the impact of heavy rainfalls, suggesting a good potential to mitigate the rainfall-induced landslides.

Elucidating factors governing MICP biogeochemical processes at macro-scale: A reactive transport model development

Microbial Induced Calcium Carbonate Precipitation (MICP) influenced by biofilm metabolism in the subsurface can be exploited for a variety of engineered applications encompassing geotechnical ground improvement, environmental bioremediation, and hydraulic barriers. A reactive transport model was developed to determine the effects of controlling factors in terms of treatment protocols and experimental methods. Six column tests were calibrated and a range for the key parameters was determined. Fifteen key parameters of MICP reactive transport model were assessed in four categories (microbial activity and attachment, sample preparation, treatment protocol, and experiment dimensions). The results emphasized the effects of three main factors of microbial activity, microbial attachment process, and number of treatment (among all 15 assessed parameters) on the calcium carbonate (CaCO3) precipitation content and distribution. An increase in specific ureolysis rate (Ku) and attachment rate coefficient (Kat) by two orders of magnitude improves average CaCO3 by up to 13% and 6%, respectively with non-uniformity (COV) increase of 16%. Higher flow rates and solution concentrations contribute to more uniform CaCO3 distribution. The constant attachment rate model is useful to yield the CaCO3 precipitation profiles but more accurate models are needed to capture exact distribution. Post-treatment hydraulic conductivity, porosity and attached biomass were assessed.

Experimental Study of MICP-Solidified Calcareous Sand Based on Ambient Temperature Variation in the South China Sea

With the continuous advancement of the construction of the Hainan Free Trade Port and Island Reef Project, deploying Microbial Induced Calcium Carbonate Precipitation (MICP technology) for related research on the temperature range in this area would be of great significance. MICP technology is an innovative and sustainable new soil reinforcement technology that uses the metabolic activity of specific bacteria to produce calcium carbonate precipitation (CaCO3) to connect loose soil. A few previous studies reporting on the applications of MICP technology in different temperature environments drew different conclusions. Therefore, this study involved MICP sand column reinforcement tests at ambient temperatures of 20 °C, room temperature, 30 °C, and 40 °C. The reinforcement effect was evaluated using indicators such as CaCO3 generation rate, Ca2+ conversion rate, bacterial adhesion rate, water absorption rate, and unconfined compressive strength, providing a reference basis for the future applications of MICP technology to island and reef engineering construction. The results showed that, with an increase of temperature from 20 °C to 40 °C, the CaCO3 production rate, Ca2+ conversion rate, and unconfined compressive strength showed a trend of first increasing and then decreasing; the UCS was 548 KPa at 20 °C and 2276.67 KPa at 30 °C; the water absorption rate at 20 °C was 25.32, which decreased continuously with increasing temperature, and reached 21.49 at 40 °C; and the bacterial adhesion rate also continued to rise in the range of 20 °C to 40 °C, from 10.91 to 28.44. The increase in temperature had an impact on the physiological state of bacterial cells. A scanning electron microscope test shows that CaCO3 crystal forms generated under different temperature environments were different, and the CaCO3 mineral deposits generated during MICP reinforcement at 30 °C were denser. Fewer gaps were present between adjacent sand particles, and the bond was tight, which served better as a bridge. The strength of the solidified sample was also higher. The annual average temperature of the South China Sea is about 30 °C. The findings of this experiment provide feasibility and sustainable development for MICP project reinforcement in the South China Sea.

Effects of hydrogel-encapsulated bacteria on the healing efficiency and compressive strength of concrete

Microbial-induced calcium carbonate precipitation is a promising technology for self-healing concrete due to its capability to seal microcracks. The main goal of this study was to evaluate the effects of adding hydrogel-encapsulated bacteria on the compressive strength and the self-healing efficiency of concrete. To achieve this objective, 12 sets of mortar samples were prepared, including three different mineral precursors (magnesium acetate, calcium lactate, and sodium lactate), at two concentrations (67.76 and 75.00 ​mM/L), and under two different biological conditions (with and without bacteria). In addition, a set of plain mortar samples was prepared to serve as a control. For each sample set, three mortar cubes and three beams were prepared and subjected to compression and flexural strength tests. From the compression tests, it was found that the sample containing calcium lactate along with yeast extract and bacteria displayed the best results. As for the flexural tests, once cracked, the beams were subjected to 28 ​d of wet/dry cycles (16 ​h of water immersion and 8 ​h of drying), where the bottom crack width was monitored (at 0, 3, 7, 14, 28 ​d of wet/dry cycles). Once the sample with the highest healing efficiency was identified (the one containing calcium lactate and hydrogel-encapsulated bacteria), the study was scaled up to concrete specimens. Two sets of concrete cylinders (consisting of three control samples and three samples with bacteria along with calcium lactate) were tested under compression in order to evaluate the effect of the bacteria-precursor combination on the concrete mechanical properties. The samples that yielded the greatest compressive strength were the ones containing calcium lactate and bacteria, displaying an improvement of 17% as compared to the control specimen. Furthermore, a flexural strength recovery analysis was performed on the concrete specimens revealing that the control showed better flexural strength recovery than the bacteria-containing variant (41.5% vs. 26.1%) after 28 ​d of wet/dry cycles. A healing efficiency analysis was also performed on the cracked samples, revealing that the control displayed the best results. These results are due to the fact that the control specimen showed a narrower crack width in comparison to the bacteria-containing samples.