Microtensile Bond Strength Test Research Articles

Extrafibrillar demineralization: Yes or no?

ObjectiveExtrafibrillar demineralization is considered to be an ideal solution for addressing the durability of resin-dentin bonding interfaces. However, its theoretical basis is contradictory to ionization equilibrium of hydroxyapatite dissolution. In this study, various calcium chelators were selected as dentin conditioners to explore the essence of dentin demineralization with chelators and its effect on resin-dentin adhesion. MethodsPolyethyleneimine grafted with EDTA and polyacrylic acid sodium (PAAN450k) larger than 40 kDa, as well as PAAN (PAAN3k) and EDTA smaller than 6 kDa, were prepared as dentin conditioners. The dentin powder was designed to characterize whether it would demineralize without contact with PAAN450k. Dentin demineralization effect with four conditioners was evaluated with field emission scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, atomic force microscopy and quantification of hydroxyproline concentration after enzymatic degradation. Micro-tensile bond strength (µTBS) test and failure mode analysis were employed to assess the bonding effect of the four chelators in both wet and dry bonding, with H3PO4 wet bonding serving as the control group. ResultsDemineralization occurs when PAAN450k was not in direct contact with the dentin powder. The extrafibrillar demineralization cannot be induced by any chelator regardless of its molecular weight. Complete demineralization including extrafibrillar and intrafibrillar demineralization would occur with sufficient interaction time. Moreover, chelators could not provide a reliable dentin bonding effect under a short interaction time. SignificanceFrom the perspective of theory and application, extrafibrillar demineralization is not a reliable strategy, which provides a reminder for exploring new strategies in the future.

Microtensile bond strength and nanoleakage of self-etching adhesives containing different concentrations of 45S5 bioactive glass in caries-affected dentin

ObjectiveTo evaluate microtensile bond strength (μTBS) and nanoleakage (NL%) of self-etching adhesive systems containing different concentrations of bioactive particles when applied to caries-affected dentin: immediately and after 1 year of storage. Materials and methodsThree experimental self-etching adhesive systems were developed: (1) Experimental control adhesive (without glass), (2) adhesive containing 5 % 45S5 glass (45S5 5 %), and (3) adhesive containing 20 % 45S5 glass (45S5 20 %). Sound human molars were used (n = 7). The dentin surface was exposed, and half of the specimens were subjected to artificial caries induction, followed by partial removal of the carious tissue. Adhesives were applied to the dentin surface, and teeth were restored with composite resin and sectioned. Specimens undertook microtensile bond strength tests in a universal testing machine (Instron 3342) at 24-h and 1-year. Interfaces of the sectioned specimens were observed under a stereomicroscope to obtain the fracture mode. For nanoleakage test, specimens were infiltrated with silver nitrate and the interface was analyzed using a Scanning Electron Microscope (SEM) at 24-h and 1-year. Data were subjected to ANOVA test (three-way) and Holm-Sidak post-hoc (α = 0.05). ResultsFor μTBS (p = 0.574) and NL (p = 0.334) tests, there was no interaction between the 3 main factors (Material vs. Substrate vs. Time). There was an interaction between dentin and material for μTBS (p < 0.001) and NL (p = 0.005) tests. Bioactive adhesives with 5 % and 20 % concentration were not influenced by the type of dentin (p < 0.05). There was a reduction in μTBS values and an increase in NL values for the adhesive without bioactive glass in dentin affected by caries (p > 0.05). ConclusionBioactive glasses had therapeutic action on dentin. The bond strength values of bioactive adhesives were maintained in caries-affected dentin and had low nanoleakage values. Clinical significanceSelf-etching adhesives containing 45S5 bioactive glass have the potential to preserve the dentin substrate and improve the longevity of restorations with composite resins.

Influence of residues from bioceramic sealer on the bond interface with universal adhesive in different application modes.

This study evaluated the effect on adhesive layer thickness, bond strength, and adhesive failure pattern of the application of universal adhesive (Scotchbond Universal) using either manual or rotary brush in dentin previously impregnated with bioceramic sealer (Sealer Plus BC) using a manual brush, at 24h and 1 year. Eighty-eight bovine crowns were divided into four groups (n=22) according to the intervention: (i) use of bioceramic sealer and adhesive application using manual brush, (ii) use of bioceramic sealer and adhesive application using rotary brush, (iii) use of resin sealer and adhesive application using manual brush, and (iv) use of resin sealer and adhesive application using rotary brush. Subsequently, specimens were restored with a composite resin (Filtek Z-250). Adhesive layer thickness was evaluated using confocal microscopy. Bond strength was assessed using the microtensile bond strength test, and adhesive failure pattern was evaluated under a stereomicroscope. Data were analyzed using two-way ANOVA/Tukey tests. Specimens where a rotary brush had been used exhibited lower adhesive layer thickness. Specimens treated with resin sealer and using a manual brush showed lower bond strength values and a higher occurrence of adhesive failures at 24h and 1 year than specimens treated with bioceramic sealer and using rotary brush for adhesive application.

Enhancing bonding of zirconia lithium silicate ceramics to dentin: The effect of surface treatment and resin cement

This laboratory study evaluated the influence of surface treatments on surface topography, and the bonding of CAD/CAM (computer-aided design computer-aided manufacturing) zirconia-containing lithium silicate (ZLS) surfaces to human dentin using various contemporary resin cements. Specimens from ZLS CAD/CAM blocks (Celtra™ Duo and Vita™ Suprinity) underwent three surface pretreatments: grinding only; 5 % hydrofluoric acid etching followed by application of a silane coupling agent, and a self-etching ceramic primer. The specimens were bonded to a flat exposed dentin surface using two etch-and-rinse and two self-adhesive resin cements, and then sectioned into microspecimens for microtensile bond strength (μTBS) testing. Failure modes of bonded surfaces, and ceramic surface roughness and topography were assessed. Results indicated that surface treatment was essential for ZLS-dentin bonding. Hydrofluoric acid and silane, along with the self-etching primer resulted in comparable strengths and outperformed the control group. Etch-and-rinse cements yielded significantly higher μTBS than self-adhesive cements. Significant differences in surface roughness and topography were observed among the treatment groups. Hydrofluoric acid exhibited the highest roughness parameters and most prominent surface changes. These findings highlight the importance of surface treatment for enhancing zirconia-containing lithium silicate bonding to dentin, and emphasize the critical role of the resin cement choice in optimizing dental restorative outcomes.

Influence of titanium dioxide nanotubes with alkyl ammonium alkyl bromide on the physicochemical properties and adhesion of experimental adhesive resins to dentin

ObjectiveThis study evaluated the effect of the incorporation of titanium dioxide nanotubes with alkyl trimethyl ammonium bromide (ntTiO2/ATAB) into experimental adhesive resins on their physicochemical properties and adhesion to dentin. Materials & methodsThe experimental adhesive resins were formulated with 66.66 wt% Bis-GMA and 33.33 wt% HEMA, photoinitiator with addition of ntTiO2/ATAB (1:1) in different concentrations: G1%, G2.5%, and G5%. One groups with no ntTiO2/ATAB addition was used as control (G0%). An optical tensiometer was used to analyze the contact angle (COA) by the sessile drop method (distilled water and α-bromonaphthalene) (n = 5), while the surface free energy (SFE) was calculated using Owens-Wendt-Rabel-Kaelble method. Flexural strength (FS) test (three-point bending) of polymerized adhesive samples was performed according to ISO 4049:2019 (n = 10) after 24 h or one year of water storage at 37 °C, as well as for the microtensile bond strength (μTBS) test, in which a commercial adhesive (GSBMP) was also used as control (n = 10). The adhesive-dentin interfaces were evaluated by scanning electron microscopy and X-ray spectrometry (n = 5). Data were analyzed by one-way (COA and SFE), two-way (FLE and μTBS) ANOVA and Tukey's test (α = 0.05). ResultsG5% showed the highest COA with nonpolar liquid and higher SFE than G0%. The G5% showed decreased FS compared to G0%. The μTBS of both controls (G0% and GSBMP) decreased after one year. TiO2/ATAB-containing adhesives to dentin showed no significant decrease after one year. All adhesives formed hybrid layer and the resin tags inside the dentinal tubules. ConclusionsThe incorporation of 1 and 2.5% of ntTiO2/ATAB into experimental adhesive resins did not impar the SFE. After one year, any concentration of ntTiO2/ATAB reduced the FS compared with control, but did not impair the μTBS.

Warm Air Delivery in Adhesive Application: Effect on Bonding Performance and Morphological Outcomes.

Solvent evaporation within an adhesive layer is a crucial step during a bonding process. The aim of this current research was to test whether the use of different air temperatures (20 °C, 40 °C, and 60 °C) for solvent evaporation improves the performance of four adhesive systems to dentin. Sixty non-carious human molar teeth were randomly prepared for micro-tensile bond strength (μTBS) tests. Four different adhesive systems, Prime&Bond Universal (PBU), OptiBond Universal (OBU), OptiBond FL (OBFL), and Clearfil SE (CSE), were applied following the manufacturer's instructions. Three groups based on the air-drying temperature were used: solvent evaporation was performed with either of warm (40 °C), (60 °C), and cold air as control group (20 °C) for 10 s at a distance of 5 cm. In all bonded surfaces, three resin composite (Reflectys, Itena Clinical, Paris, France) layers of 2 mm thickness were built up. The resin-dentin samples were kept in distilled water at 37 °C for 24 h and 6 months, respectively, before μTBS testing. Failure analysis, scanning electron microscopy of resin-dentin bonded interface, and solvent evaporation rate were tested as secondary variables. All analyses were conducted using a significance level of α = 0.05. Bond strength (BS) values were similar among all the adhesive systems used (p > 0.05). Also, the aging factor did not affect the BS (p > 0.05). Only the factor of temperature used for solvent evaporation resulted in a statistically significant effect (p < 0.05), with the temperature of 60 °C being the highest value (p < 0.05). A failure mode evaluation revealed mostly adhesive or mixed modes of failures in all the different temperatures of air used for the solvent evaporation of each adhesive system. The thickness of the adhesive layer and the creation of resin tags varied amongst the temperatures evaluated. For all adhesive systems tested, the use of 40 °C or 60 °C air for solvent evaporation led to an increased mass loss. Warmer temperatures for solvent evaporation contributed positively to bonding performance, enhancing both the quality of the adhesive layer and its interaction with the dentin tissue. Optimizing solvent evaporation with warmer air temperatures (40 °C and 60 °C) significantly improved µTBS, offering a practical means to enhance the quality and longevity of adhesive restorations in esthetic dentistry.

Adhesion and sealing of different universal adhesive systems associated with bulk-fill resins after using endodontic irrigation solutions: An invitro study.

This study aimed to evaluate the interface of universal adhesive (UA) with bulk-fill resin (BF) and dentin, after endodontic irrigation. The pulp chamber of 96 molars was irrigated with chlorhexidine 2% (CHX) or sodium hypochlorite 5.25% (NaOCl) and restored according to three restorative protocols. Microtensile bond strength (μTBS) and nanoleakage tests were performed after 24 h of thermocycling. Kruskal Wallis, Mann-Whitney and Wilcoxon were performed (p < 0.05). Comparing restorative systems, a difference was found only when irrigated with CHX after 24 h. CHX provided higher μTBS than NaOCl after 24 h for two restorative protocols; after thermocycling, NaOCl provided the greatest μTBS than CHX for one group. Decreased μTBS was observed for all restorative systems when CHX was used after thermocycling. NaOCl demonstrated the highest nanoleakage. The composition of UA influenced the immediate adhesion and sealing to BF when CHX was used; ageing has a deteriorating effect on adhesion and sealing for all restorative systems.

Effect of thiol compound on the dentin bond strength when using experimental one-bottle self-etch adhesive system

This study evaluated improvements in the micro-tensile bond strength of a one-bottle adhesive to dentin upon incorporating a thiol compound. Formulations containing 0, 0.1, 0.3 or 0.5% of the thiol were prepared (TH78-0, TH78-0.1, TH78-0.3 and TH78-0.5). Flat dentin surfaces were produced on non-carious extracted human molars using a diamond saw and 600 grit SiC paper. Each surface was subsequently treated with one of the four experimental adhesives or a commercially available adhesive (Scotchbond Universal Adhesive), following the manufacturers’ instructions. A resin composite was then incrementally built on the adhesive-treated surfaces and light-cured, after which the bonded specimens were sectioned and subjected to micro-tensile bond strength tests. Bond strengths ranging from 28.7 MPa (for the TH78-0.5) to 45.9 MPa (for the TH78-0.1) were obtained from the experimental specimens, whereas the commercial adhesive showed a strength of 36.9 MPa. A 0.1% thiol concentration therefore increased the baseline bonding of the adhesive and gave the highest strength, with significantly decreased values in the cases of 0.3% and 0.5% concentrations.

Effect of silver diamine fluoride on the longevity of the bonding properties to caries-affected dentine

ObjectivesThis study aimed to evaluate the adhesive properties in dentine after the application of silver diamine fluoride (SDF) on carious dentine lesions immediately and after 2 years of water storage. Methods96 human molars used were subjected to artificial dentine caries production, and then randomly divided into 12 experimental groups according to 1. application of an SDF solution (carious dentine lesion without SDF treatment [control], with 12 % silver diamine fluoride [SDF 12 %] or 38 % silver diamine fluoride [SDF 38 %]); 2. Universal adhesives (Clearfil Universal Bond Quick [CUQ] and Single Bond Universal [SBU]); 3. adhesive strategy (etch-and-rinse [ER] and self-etch [SE]). After restoration, the specimens were sectioned and submitted to the microtensile bond strength test (μTBS) and energy-dispersive X-ray spectrometry analysis (SEM/EDX). All tests were performed immediately and after 2 years of water storage. Data from the μTBS were analyzed using four-way ANOVA and Tukey's test (α = 0.05). ResultsOnly the interaction of factors 'SDF' vs 'time' was significant (p = 0.03). After 2 years of storage, the groups where SDF was applied showed higher μTBS values compared to the control group. No significant decrease in μTBS values was observed for SBU when comparing immediate and 2-year results, but a significant reduction in μTBS values was observed after 2 years for CUQ. ConclusionIndependent of the adhesive strategy, the use of SDF may be a promising alternative to maintain the bonding of universal adhesives to carious dentinal lesions. Clinical significanceThis study may clarify and support clinicians regarding the longevity of resin-based restoration in caries-affected dentine treated with silver diamine fluoride.

Saliva with different concentrations of enzyme with esterolytic activity, and its effect on the immediate and long-term bond strength of adhesive systems to dentin

The present study evaluated whether different concentrations of albumin would influence the immediate or long-term bond strength of adhesive systems to dentin. Human third molars (N = 90) were sectioned at the dentin-enamel junction to obtain middle dentin occlusal surfaces. After sectioning into discs, the molars were restored with resin composite (Filtek Z250, 3 M/ESPE) by using an etch-and-rinse (Adper Scotchbond Multi-Purpose, 3 M/ESPE) or a self-etch (Clearfil SE Bond, Kuraray) adhesive system. The specimens were then sectioned into beams and stored in artificial saliva containing albumin in regular (100 μg/mL; SA100) or elevated (700 μg/mL; SA700) concentration—both concentrations simulated the range in which this enzyme may be present clinically, depending on the patient's periodontal condition. Beams from the control group (CO) were kept in artificial saliva with no addition of albumin. Half of the beams from each group were stored for 24 h, and the remainder, for 12 months. Then, the microtensile bond strength test was performed, and the failure mode was analyzed (adhesive, cohesive, or mixed). Bond strength data were submitted to the generalized linear model, followed by the Tukey tests, and failure modes were compared using chi-square tests (α = 5%). There were no significant interactions, but there was a significant effect between the albumin concentration (p = 0.015) and the time factor (p = 0.016). The beams stored in SA700 had significantly lower bond strength in comparison with the CO beams, regardless of the adhesive system. At 12 months, the bond strength decreased significantly for all groups. SA700 caused a significant increase in the adhesive failures after 12 months (p = 0.005). It was concluded that the bond strength of both adhesive systems was impaired by the albumin concentration aging.

Effect of zinc nanoparticles on the mechanical properties and stability of the resin-dentin bonded interface

To evaluate the effect of zinc oxide and zinc fluoride nanoparticle solution (ZNS) as pretreatment on the mechanical properties and stability of resin-dentin bonded interface. Pretreatment solutions were applied after phosphoric acid: 5 and 10% Zinc Oxide (ZO5; ZO10); 1.5 and 3% Zinc Fluoride (ZF1.5 and ZF3); 0.2% chlorhexidine (CHX-positive control) and no pretreatment (NC-negative control). The treated teeth were restored and stored for 24 h and 12 m prior to microtensile bond strength (µTBS) testing (n = 8). Failure mode, nanohardness (NH) and young’s modulus (YM) (n = 3) were also evaluated. In situ zymography (n = 3) and interface morphology (n = 3) were evaluated after 24 h. The antibacterial activity was determined by minimum inhibitory (MIC) and bacterial (MBC) concentration. The µTBS was evaluated by two-way mixed ANOVA and NH and YM data were analyzed by two-way mixed repeated-measures ANOVA (α = 0.05). In situ zymography, interface morphology, MIC and MBC were descriptively analyzed. The µTBS of ZF3, ZO10 and CHX was maintained over time, while for ZF1.5 and Z05 it was increased. Mixed failures were the most prevalent for all groups. ZF3 presented higher values of NH and YM with incorporation of nanoparticles being observed within the hybrid layer on the interface morphology image. CHX presented the lowest enzymatic activity followed by ZO5. MIC and MBC for ZO were 62.5 µg/mL and ZF 3.25 µg/mL, respectively. Pretreatment with ZNS, specially zinc fluoride, seems to be a promising method to increase the longevity of restorations.

Dentin adhesion of bulk-fill composites and universal adhesives in class I-cavities with high C-factor

ObjectivesThe aim of this study was to compare the dentin adhesion of bulk-fill composites in high C-factor class I-cavities before and after thermocycling to a control group using incremental layering technique. MethodsA standardized class I-cavity was prepared into 195 human molars, then different universal adhesives were applied either in self-etch or etch & rinse mode, and the cavity was filled according to each materials application protocol. The material combinations used were a conventional layered composite as control, the respective bulk-fill product, two other bulk-fill composites made by different manufacturers, with one of them being tested using two different polymerization times. Furthermore, one thermoviscous bulk-fill composite and one self-adhesive restorative were examined of which the latter can only be applied in self-etch mode. In each group the dentin adhesion to the cavity bottom was measured using microtensile bond strength test initially (24 h water storage) and after thermocycling. All results were statistically analyzed using STATA 17.0. ResultsThe statistical analysis showed significant differences between the control and the experimental groups (p < 0.001). The highest mean bond strength before (14.8 ± 10.7 MPa) and after aging (14.2 ± 11.5 MPa) was measured for the etch & rinse-control group. Among the bulk-fill groups, the etch & rinse technique consistently showed higher bond strengths. Bond strength of groups with shortened polymerization did not exceed 2.1 MPa. The bond strength of the self-adhesive restoration material was low before and after thermocycling (2.7 MPa/ 0.0 MPa). Groups with low bond strength values showed a high number of pre-testing-failures. ConclusionsBulk-fill materials used in high C-factor class I-cavities showed lower bond strength during self-etch application. The same applies for a shortened polymerization regime, which cannot be recommended for high C-factor cavities. Clinical significanceToday, a large variety of materials and application techniques can be used when placing an adhesive restoration. Whether new instead of established procedures should be applied in high C-factor cavities has to be critically assessed, as they are a demanding scenario for adhesive restorations.

Bond Strength, Microleakage, Microgaps, and Marginal Adaptation of Self-adhesive Resin Composites to Tooth Substrates with and without Preconditioning with Universal Adhesives.

This study investigated and compared the bond strengths, microleakage, microgaps, and marginal adaptation of self-adhesive resin composites (SAC) to dentin with or without universal adhesives. Dentin surfaces of 75 molars were prepared for shear and microtensile bond strength testing (SBS and µTBS). Silicon molds were used to build up direct restorations using the following materials to form 5 groups: 1. Surefil One; 2. Prime&Bond active Universal Adhesive + Surefil One; 3. Vertise Flow; 4. OptiBond Universal + Vertise Flow; 5. Scotchbond Universal + Filtek Z500 (control group). Bonded specimens were thermocycled 10,000x before being tested either for SBS or µTBS using a universal testing machine at a crosshead speed of 0.5 mm/min. Direct mesial and distal class-II cavities were created on 100 sound premolars, with the gingival margin of distal cavities placed below CEJ and restored according to the five groups. After thermocycling, microleakage scores were assessed following immersion of restored premolars in 2% methylene blue dye for 24 h, while marginal gaps and adaptation percentages were investigated on epoxy resin replicas under SEM at magnifications of 2000X and 200X, respectively. Results were statistically analyzed with parametric and non-parametric tests as applicable, with a level of significance set at α = 0.05. Bond strengths, microleakage scores, microgaps, and percent marginal adaptation of Surefil One and Vertise Flow were significantly (p < 0.001) inferior to the control group. Dentin preconditioning with universal adhesives significantly increased the study parameter outcomes of Surefil One and Vertise Flow, yet they were still significantly below the performance of the control group. Conventional resin composite outperformed the SAC whether applied solely or in conjunction with their corresponding universal adhesives.

Comparison of Adhesion Performance of a Self-curing and a Light-curing Universal Adhesive to Various Dental Substrates and CAD/CAM Materials.

To compare the adhesion of a self-curing (Tokuyama Universal Bond, TUB) and a light-curing (Scotchbond Universal, SBU) universal adhesive to CAD/CAM materials, enamel, and dentin. This study also assessed differences in enamel adhesion between self-etch vs selective etching modes, as well as immediate and long-term adhesion to dentin for both adhesives. Shear bond strength (SBS) testing was used to assess adhesion to enamel, dentin, Lava Ultimate (LU), Vita Enamic (VE), IPS e.max CAD (LD), IPS e.max ZirCAD (3Y-Zir), and Lava Esthetic (5Y-Zir) (n = 10). Moreover, bonding to enamel in self-etch and selective etching modes (n = 10) as well as immediate and aged resin-dentin bond strength (24 h after bonding, after 100,000 thermal cycles [TC] and long-term storage) was evaluated using the microtensile bond-strength test (n = 30). Failure mode was also determined for the bonding to dentin. Statistical analyses consisted of one-way and two-way ANOVA with appropriate post-hoc Tukey-Kramer or two-sample t-tests, as well as the chi-squared or Fisher's exact test (α = 0.05). TUB and SBU universal adhesives presented similar bonding to LU, VE, 3Y-Zir, and 5Y-Zir. However, SBS for TUB was superior to SBU when bonding to lithium-disilicate glass-ceramic (IPS e.max CAD). SBU showed better adhesion to dentin and enamel when used in the self-etch mode, while TUB promoted strong bond strength to enamel in the selective etching mode. TUB after TC was the only aging condition that yielded a significant reduction in resin-dentin bond strength. In-vitro adhesion performance of the self-curing and light-curing universal adhesives varies depending on the dental substrate or CAD/CAM restorative material used for bonding.

Advanced statistical analyses to reduce inconsistencies in bond strength data focused on donor factors: A six-factor analysis using linear mixed and nonlinear regression models.

This study aimed to investigate the effects of the age and sex of tooth donors on dentin bond strength. A total of 38 extracted teeth (12 male and 26 female donors; age range: 17-82 years) were used in this study. In addition to donor age and sex, four other microtensile bond strength (μTBS) test specimen factors were evaluated: dentin position, bonding area, presence of voids at the interface, and computed tomography (CT) values of dentin. The μTBS was measured immediately (24 h) and 6 months after storage in water. After the μTBS testing, linear mixed and nonlinear regression models were used to analyze the effects of these factors on the μTBS data. The results from the linear mixed model revealed that the bonding area (P = 0.02), presence of voids at the interface (P = 0.04), and storage time (P < 0.001) significantly affected bond strength. In contrast, no correlation was observed between the μ TBS and dentin position (P = 0.08) or sex (P = 0.07). The results of the nonlinear regression model with robust variance-covariance estimators revealed that age significantly affected bond strength (P < 0.001). In addition, a significant positive correlation was found between μTBS and age (P < 0.001), with nonlinearity (P = 0.002). However, no correlation was observed between the μTBS and CT values (P = 0.69) without nonlinearity (P = 0.39). These findings suggest that bond strength increases with age until 60 years but not afterward.

Effect of Phytic Acid Etching and Airborne-Particle Abrasion Treatment on the Resin Bond Strength.

This study aimed to evaluate the bond strength of a universal adhesive to dentin (μTBS) using different time periods of airborne particle abrasion (APA) and two types of acid etching. Seventy-two human third molars were divided into 9 groups (n=8) according to dentin pretreatment: APA duration (0, 5, or 10s) and acid etching (no acid - NA, 37% phosphoric acid - PhoA, or 1% phytic acid - PhyA). APA was performed at a 0.5 cm distance and air pressure of 60 psi using 50 μm aluminum oxide particles. Afterwards, two coats of Single Bond Universal adhesive (3M) were applied to the dentin surface. Composite blocks were built using the incremental technique, sectioned into 1×1 mm slices and subjected to microtensile bond strength (μTBS) testing. Fracture patterns and surface topography of each dentinal pretreatment were evaluated using a Scanning electron microscope (SEM). Bond strength data were analyzed using two-way ANOVA and Bonferroni post-hoc tests. The group that received pretreatment with 5s APA and PhoA presented higher μTBS values among all groups, which was statistically different when compared with the PhoA, 10APA+PhoA, and 5APA+PhyA groups. PhyA did not significantly influence the bond strength of the air-abraded groups. Finally, adhesive failure was considered the predominant failure in all groups. Dentin pretreated by airborne particle abrasion using aluminum oxide demonstrated an increase in bond strength when abraded for 5 seconds and conditioned with phosphoric acid in a universal adhesive system.

Effects of immersion in 4-methacryloyloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane resin-activated liquid on microtensile bond strength of root canal dentin

Background/purpose4-methacryloyloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane (4-META/MMA-TBB) resin is used for indirect restorations. We aimed to evaluate effects of immersion in 4-META/MMA-TBB-activated liquid on the bond strength of root canal dentin. Materials and methodsWe used freshly extracted single-rooted human teeth. After decoronation, each root was vertically sectioned into halves; their dentin walls were polished and flattened. The control group underwent dentin treatment with Green Activator. The immersion group was treated with Green Activator and Teeth Primer and immersed in 4-META/MMA-TBB-activated liquid. After bonding the resin blocks with Super-Bond, microtensile bond strength (μTBS) tests were performed (n = 6), and fracture surfaces were analyzed. Before surface treatment, dentin was immersed in a sodium fluorescein solution for 3 h, and resin blocks were bonded with Super-Bond with rhodamine B as in the bond strength test. The bonded cross section was observed using confocal laser scanning microscopy (CLSM). ResultsμTBS was significantly higher in the immersion group than in the control group (61.5 [51.3–66.7] vs. 33.0 [20.4–57.8] MPa; P < 0.05). Fracture mode analysis showed that, compared with the control group, the immersion group had a significantly lower rate of adhesive failure at the dentin interface and a significantly higher rate of cohesive failure in Super-Bond (P < 0.01). CLSM showed a water droplet-like accumulation of fluorescein dye above the hybrid layer in the control group, not in the immersion group. ConclusionImmersion in a 4-META/MMA-TBB-activated liquid inhibited water exudation from the root canal dentin and improved the bond strength.

Effects of alpha-tocopherol antioxidant on fracture strength and adhesion of endodontically treated teeth restored after dental bleaching.

This study evaluated the effect of different concentrations of alpha-tocopherol in gel form on fracture strength, hybrid layer formation, and microtensile bond strength of endodontically treated teeth bleached with 40% hydrogen peroxide (H2 O2 ). Sixty bovine incisors were randomized into one of six groups (n=10 incisors per group) defined by the interventions carried out after endodontic treatment. In the control group, no additional intervention was carried out, while all teeth in the five intervention groups were bleached with 40% H2 O2 and subsequently treated with alpha-tocopherol at concentrations of 15% (15AT), 20% (20AT), or 25% (25AT), with 10% sodium ascorbate (10SA), or with nothing (40HP). Fracture strength was evaluated in a mechanical testing machine, hybrid layer formation was assessed using scanning electron microscopy, and bond strength was determined using microtensile bond-strength testing. Data were analyzed using Kruskal-Wallis and Dunn's tests. No statistically significant difference regarding fracture strength was observed among groups. Hybrid layer formation was greater in the 15AT group than in groups 40HP and 10SA. Teeth in groups 15AT, 20AT, and 25AT demonstrated higher bond strength than teeth in groups 40HP and 10SA. Alpha-tocopherol, preferably at 15%, effectively reverses the deleterious effects, of bleaching, on hybrid layer formation and bond strength to dentin.

A glycol chitosan derivative with extrafibrillar demineralization potential for self-etch dentin bonding

ObjectivesExtrafibrillar demineralization is an etching technique that removes only minerals from around the collagen fibrils for resin infiltration. The intrafibrillar minerals are left intact to avoid their replacement by water that is hard for adhesive resin monomers to displace. The present work reported the synthesis of a water-soluble methacryloyloxy glycol chitosan-EDTA conjugate (GCE-MA) and evaluated its potential as an extrafibrillar demineralization agent for self-etch dentin bonding. MethodsGlycol chitosan-EDTA was functionalized with a methacryloyloxy functionality. Conjugation was confirmed using Fourier transform-infrared spectroscopy. The GCE-MA was used to prepare experimental self-etch primers. Extrafibrillar demineralization of the primers was evaluated with scaning electron microscopy and transmission electron microscopy. The feasibility of this new self-etch bonding approach was evaluated using microtensile bond strength testing and inhibition of dentin gelatinolytic activity. The antibacterial activity and cytotoxicity of GCE-MA were also analyzed. ResultsConjugation of EDTA and the methacryloyloxy functionality to glycol chitosan was successful. The functionalized conjugate was capable of extrafibrillar demineralization of mineralized collagen fibrils. Tensile bond strength of the experimental self-etch primer to dentin was comparable to that of phosphoric acid-etched dentin and the commercial self-etch primer Clearfil SE Bond 2. The GCE-MA also inhibited soluble rhMMP-9. In-situ zymography detected minimal fluorescence in hybrid layers conditioned with the experimental primer. The GCE-MA was noncytotoxic and possessed antibacterial activities against planktonic bacteria. SignificanceSynthesis of GCE-MA brought into fruition a self-etch conditioner that selectively demineralizes the extrafibrillar mineral component of dentin. A self-etch primer prepared with GCE-MA achieved bond strengths comparable to commercial reference adhesive systems.

Effect of the prism-interprisms three-dimension spatial microstructure on the enamel bond strength.

The prism-interprisms level of the enamel hierarchical microstructure is the largest degree of structural variation and most sophisticated structural adaptation. We studied the effect of the prism-interprisms three-dimension spatial microstructure on the enamel bond strength. We prepared 11 groups of enamel segments: longitudinally sectioned segments with or without a 45-degree bevel (group = 2), horizontally sectioned segments with or without a 45-degree bevel of three regions (the incisal, middle, and cervical) (group = 6), and tangential (labial) sectioned segments of three regions (the incisal, middle, and cervical) (group = 3). The finished surface of each segment was observed by scanning electric microscopy (SEM) before treatment with four self-etch adhesive systems and applied with four corresponding composite resins. Resin-bonded enamel samples were prepared in beams for microtensile bond strength (MTBS) tests. The results were analyzed with a three-way ANOVA followed by Tukey's post-hoc HSD multiple comparisons procedure. SEM observations revealed complex arrangements of prisms and interprisms. MTBS measurement showed that the longitudinally sectioned group had the lowest value, without significant differences between the groups with or without 45-degree bevel. Combining SEM observations and MTBS measurements, the prism-interprisms microstructure varied with the incisor regions, and different prism-interprisms microstructures allowed diverse sectioned surfaces, which could affect the enamel bonding.