Micro-teaching aims at making pre-service teachers adopt and acquire the roles and behaviours they will have in the classroom with success motivation. Without viable teaching aptitude, success will be merely a wishful thinking. Therefore, this study explores the influence of micro-teaching on the teaching aptitude of Business Education students in Colleges of Education in South West Nigeria. The study adopted survey research design of ex-post facto type and the population comprised of all NCE III students in Business Education Department during 2019/2020 academic session that were posted to the field for teaching practice in all state and federal owned Colleges of Education in South-West Nigeria. A total number of 1360 student teachers in business education participated in the study using multistage sampling technique. Two research questions and hypothesis were answered and tested respectively in the study. Two validated instruments were used for data collection; Students Micro-teaching Assessment Rating Scale (SMARS) and Micro-teaching on Teaching Aptitude Questionnaire (MTAQ) (α=0.96). Data were analyzed using descriptive statistics and Multiple Regression. Findings revealed that under the established norms and to a very great extent teaching aptitude was very high among Business Education students. The finding further showed that micro-teaching significantly contributes (F (1, 1358) = 4.484, p < .05, ß =.132 t (1358) = 3.676, p< 0.05) to teaching aptitude of Business Education students. The study concluded that micro-teaching is one of the most influential factors that enhanced Business Education students in Colleges of Education level of teaching aptitude and performance in teaching practice. It was therefore recommended, among others, that the College management should enhanced and sustain a healthy micro-teaching courses to achieve its mission objectives and boost aptitude to teaching of pre-service teachers nationwide.