MI Varnish Research Articles

Impact of Cinnamon and Cloves Extracts Incorporated in Fluoride Varnish Containing CPP-ACP on Microhardness and Color of Enamel

Objective: The current study aimed to evaluate the effects of methanolic extracts of Cinnamon and Cloves mixed with Fluoride Varnish Containing CPP-ACP (MI Varnish™) treatments on the enamel microhardness and color. Material and Methods: Forty enamel specimens obtained from twenty human impacted third molars were randomly divided into four groups (n = 10) according to surface treatment: (G1) MI Varnish™ mixed with methanolic extract of Cinnamon; (G2) MI Varnish™ mixed with methanolic extract of Cloves; (G3) MI Varnish™ mixed with methanolic extracts of Cinnamon and Cloves meanwhile (G4) MI Varnish™ alone. Specimens were subjected to pH cycling. Color (ΔE) and surface microhardness were measured for each specimen at base line, after demineralization and post treatment. Results: The highest microhardness values presented in MI Varnish alone (G4) and MI Varnish™ mixed with methanolic extracts of Cinnamon and Cloves (G3) without any significant difference between them, followed by MI Varnish™ mixed with methanolic extract of Cinnamon (G1), while the lowest hardness values were found in MI Varnish™ mixed with methanolic extract of Cloves (G2). There was an increase in ΔE3 values for all groups. Conclusions: Under the limitations of the current study, application of Cinnamon and Clove as herbal antibacterial medicaments does not affect enamel microhardness negatively when added with the MI paste at certain ratio. Meanwhile, unacceptable color changes of enamel surface are expected.

MI and PreviDent Varnish on Non Cavitated Interproximal Lesions. Randomised Clinical Trial

MI and PreviDent Varnish on Non Cavitated Interproximal Lesions. Randomised Clinical Trial

REMINERALIZATION EFFECT OF FLUORIDE VARNISH CONTAINING CASEIN PHOSPHOPEPTIDE AMORPHOUS CALCIUM PHOSPHATE ON CARIES-LIKE LESIONS IN PRIMARY TEETH (IN VITRO STUDY)

INTRODUCTION: Fluoride containing casein phosphopeptide amorphous calcium phosphate (CPP-ACP) varnishes are now available as (MI Varnish). It has an effect on the remineralization process of demineralized enamel, since it has the highest release of calcium and fluoride ions. OBJECTIVES: To study the remineralizing effect of fluoride varnish containing casein phosphopeptide amorphous calcium phosphate (MI varnish) on the remineralization of caries-like lesions in primary teeth compared to sodium fluoride varnish. MATERIALS AND METHODS: Twenty-four freshly extracted primary maxillary incisors with standardized 4x4 windows were immersed in demineralizing solution for four days. Teeth were divided into two groups, group I (n=12 teeth); MI varnish and group II (n=12 teeth); Prevident varnish. They were sectioned into two halves in a buccolingual direction (48 specimens). One half was treated with the remineralizing agent and the other half remained untreated and served as its control. Specimens were assigned into subgroup I A (MI varnish) n=12 specimens, subgroup I B (untreated) n=12 specimens, subgroup II A (Prevident varnish) n=12 specimens and subgroup II B (untreated) n=12 specimens. Specimens were subjected to pH cycling for ten days. The specimens were evaluated quantitatively using energy dispersive x-ray spectrometer (EDX). RESULTS: MI varnish treated specimens (subgroup I A) showed significant high mean Ca, Ca/P ratio than the untreated specimens. Also, Prevident varnish treated specimens showed significant high mean Ca, Ca/P ratio than the untreated specimens. Median percent change of Ca/P ratio of MI varnish treated specimens was significantly higher than Prevident varnish treated specimens. CONCLUSIONS: Fluoride varnish containing CPP-ACP has a higher remineralization potential of the caries-like lesion in primary teeth in comparison to sodium fluoride varnish.

In Vitro Influence of Prophylaxis Cleaning on Enamel Remineralization with Casein Phosphopeptide-Amorphous Calcium Phosphate.

ABSTRACTObjectivesThis randomized controlled in vitro 4-arm trial study aimed to study the remineralization potential of Recaldent™ and assess the effects of prophylaxis cleaning and MI Varnish® on enhancing this remineralization potential.Material and MethodsSixty human teeth were randomly assigned into equal samples (A/B). Sample A was prophylactically cleaned, randomly divided into equal samples (1/2). Sample A1 received treatment with MI Varnish® and Recaldent™ for 30 days. Sample A2 was treated similarly but without MI Varnish®. Sample B did not receive prophylaxis cleaning and was divided into equal samples (1/2). Sample B1 was treated as A1 and sample B2 as A2. The teeth were examined for mineral composition at baseline, after the interventions (T1), and after prophylaxis cleaning (T2). Study outcomes were mineral content (% weight of carbon [C], phosphorus [P], calcium [Ca], oxygen [O], chlorine [Cl], sodium [Na] and silicon [Si]) and calcium-phosphorus ratio (Ca/P).ResultsAll groups had similar mineral composition at baseline. At T1, sample B2 exhibited least P, Ca and Ca/P content. Samples A1 and B1 showed higher content of P and Ca, compared to B2 (A1 only exhibited higher Ca/P). Sample A2 exhibited lowest Cl and Na content. At T2, sample A1 exhibited lowest C, P, O and Si content (highest Ca/P). Sample A2 showed least Ca/P, and highest Na content.ConclusionsTeeth treated by Recaldent™ proceeded by prophylaxis cleaning or MI Varnish® showed remineralization, especially when receiving both interventions. This superior effect persisted even after a second cleaning. Further trials are necessary to provide conclusive evidence in humans.

Analysis of efficacy of the self-assembling peptide-based remineralization agent on artificial enamel lesions.

The objective of this in vitro study was to analyze and compare the biomimetic remineralizing efficacy of the self-assembling peptide (P11-4) with agents containing casein phoshopeptide-amorhous calcium phosphate fluoride (CPP-ACFP) and sodium fluoride (NaF) on artificial caries lesions using DIAGNOdent and micro-computed tomography (μCT). Artificial enamel lesions were prepared on extracted impacted sound mandibular third molars. The samples were randomly allocated to four groups (n = 8): Group 1, P11-4 (Curodont Repair, Credentis AG, Switzerland); Group 2, CPP-ACFP (MI Varnish, GCCo., Japan); Group3, NaF (Duraphat Varnish, Colgate, Colgate-Palmolive, NY, USA); Group 4, artificial saliva (control). The agents were applied to demineralized surfaces according to manufacturers' instructions; all specimens were stored in artificial saliva for 1 month. Demineralization and remineralization on enamel surfaces were analyzed and quantified by DIAGNOdent (KaVo, Germany) and μCT (SkyScan1174, Belgium) for lesion depth/area/volume/mineral density (MD). The remineralization efficacy of the agents was evaluated by DIAGNOdent on 1st, 7th, 30th days and by μCT on 30th day. Data were statistically analyzed by ANOVA, Kruskal-Wallis, T test, and Wilxocon tests. The highest remineralization efficacy findings in all periods were determined in Group 1, followed by Groups 2, 3, and 4. The remineralization findings for fluorescence, MD, lesion depth in Group 1 were found significantly higher (p < 0.01) than Group 3; and no significant differences (p > 0.05) were found between Groups 1-2 and Groups 2-3. The area and volume change values in Groups 1, 2, and 3 have shown no significancy (p > 0.05). A significant correlation (p < 0.01) was found between μCT and DIAGNOdent methods. The data of this study have demonstrated that P11-4 has showed the best remineralization efficacy, followed by CPP-ACFP and NaF. It is concluded that self-assembling peptide-based remineralization agent can be used successfully for biomimetic remineralization of enamel subsurface lesions.

Effects of treatment with three types of varnish remineralizing agents on the microhardness of demineralized enamel surface.

BackgroundRemineralization of incipient caries is one of the goals in dental health care ,especially in pediatric dentistry. The present study aimed at comparing the effects of MI varnish (3M (United states)) , Nano paste( FGM(Brezil) ), 5% sodium fluoride varnish) DuraphatColgate (united states) ) on remineralization of enamel lesions.Material and MethodsIn this in-vitrostudy, 60 intact human pre-molars, were randomly allocated to four groups of 15. Baseline surface microhardness in three points in the center of the polished area was measured. After two days of immersion in demineralizing solution, microhardness of all samples was measured. Afterward, groups 1-3 under-went treatment with MI varnish, nano paste, 5% sodium fluoride varnish and then again microhardness was measured. The results were analyzed by one-way analysis of variance (ANOVA), repeated measures ANOVA, and Bonfreni table was used.ResultsDuraphat varnish in comparison with control group, significantly increased surface microhardness and in comparison with Nano and MI paste varnish groups significant differences was shown between groups. (P< 0.05). MI paste varnish and Nano paste similary showed more increases in surface microhardness in comparison with Duraphat varnish and control groups (P≈1).ConclusionsAccording to the results of this study, all three varnishes, Duraphat , MI paste and Nano paste increase the enamel surface microhardness and remineralization of incipient caries. MI paste and Nano paste compared to Duraphat Varnish, significantly showed more increases in enamel surface microhardness but Nano paste and MI paste were almost the same. Key words:CPP-ACP, Nano varnish, fluoride varnish, microhardness, demineralization, remineralization.

CPP-ACP and Fluoride: A Synergism to Combat Caries.

ABSTRACTAimsThe aim of this study is to evaluate and compare the demineralization inhibitory effect, fluoride release at varying time intervals, and antibacterial property of MI varnish and fluor protector varnish.Materials and methodsTwenty-four extracted human premolars were sectioned mesiodistally. The buccal and the lingual halves of the teeth were utilized to evaluate the demineralization inhibitory effect and fluoride release of MI varnish and fluor protector varnish, respectively. To evaluate the demineralization inhibitory effect, 150-μm axial longitudinal section was obtained that was photographed and evaluated under a polarized light microscope. To evaluate the fluoride release, a fluoride-specific ion electrode was used. To evaluate the antibacterial susceptibility, the disk diffusion test (Kirby–Bauer method) was done.ResultsStatistical analysis was done using the Students unpaired t test for intergroup comparison and the p value <0.01 was obtained for all the three parameters, i.e., demineralization inhibitory effect, antimicrobial property, and fluoride releasing property at different time intervals.ConclusionA combination of casein phosphopeptide (CPP)–amorphous calcium phosphate (ACP) in fluoride varnish seems to enhance the caries preventive potential of fluoride varnish and antibacterial activity against Streptococcus mutans.Clinical significanceMI varnish, a combination of CPP–ACP and fluoride varnish could be the future of minimally invasive dentistry as it is minimally invasive yet an effective modality for caries prevention.How to cite this articleAttiguppe P, Malik N, et al. CPP–ACP and Fluoride: A Synergism to Combat Caries. Int J Clin Pediatr Dent 2019;12(2):120–125.

Comparative Evaluation of Remineralization Potential of Two Varnishes Containing CPP-ACP and Tricalcium Phosphate: An In Vitro Study.

ABSTRACTAimThe aim of this study is to evaluate the remineralization potential of Clinpro XT varnish containing tricalcium phosphate (TCP) and MI varnish containing casein phosphopeptide (casein phosphopeptide–amorphous calcium phosphate, CPP–ACP).Materials and methodsThirty premolar teeth were taken and divided into three groups. Samples were sliced mesiodistally into buccal and lingual halves using a diamond disk bur. The buccal halves of the teeth were used for the study. Artificial caries like lesions were produced and evaluated with Diagnodent. The samples in each group were treated with the respective remineralizing agent (except for the control group) at every 24 hours for 7 days and the surfaces were assessed using Diagnodent to record the values after the remineralization procedure. The Diagnodent values obtained were tabulated and statistically analyzed using one-way ANOVA and Tukey's multiple comparison tests.ResultsThere was a significant difference between the values calculated before and after remineralization in all the three groups.ConclusionThe study findings showed that MI varnish containing CPP–ACP had the highest release of fluoride as compared to the Clinpro fluoride releasing varnish.Clinical significanceMI varnish is a 5% NaF varnish containing CPP–ACP to give an exceptional fluoride varnish that releases more bioavailable fluoride, calcium, and phosphate, hence, can be used successfully in remineralization of early carious lesions. CPP–ACP can be used in the clinical practice for reversing or arresting the early carious lesions.How to cite this articleVarma V, Hegde KS, et al. Comparative Evaluation of Remineralization Potential of Two Varnishes Containing CPP–ACP and Tricalcium Phosphate: An In Vitro Study. Int J Clin Pediatr Dent 2019;12(3):233–236.

The effect of different re-mineralizing agents and diode laser irradiation on the microhardness of primary molar enamel: An in vitro study.

Dental caries is a global concern and different materials and methods were proposed for its prevention.The aim of this study was evaluation of the effect of different demineralizing agents with and without diode laser radiation on the microhardness of primary molars enamel. 48 primary molar teeth were used in this study. The enamel blocks were prepared. Primary microhardness values were also measured. All samples were demineralized, then demineralization was approved by a DIAGNOdent pen. The remineralization was done into the buccal and lingual surfaces of the samples as follows:Group 1: CPP-ACP, Group 2: CPP-ACPF, Group 3: MI varnish, Group 4: NaF varnish. The lingual surfaces received diode laser irradiation. Finally, a secondary microhardness test was performed on all samples. Statistical analyses were done and a P-value less than 0.05 was considered to be statistically significant. A comparison between the primary and the secondary microhardness values showed significant differences (P = 0.003). The highest secondary microhardness values belonged to the MI varnish +laser group. Application of the agents, containing the combination of Ca, P, and F, was significantly better than others in increasing the microhardness of the enamel. Although the application of diode laser increased the hardness of the enamel.

Effect of Er:YAG and Fluoride Varnishes for Preventing Primary Enamel Demineralisation.

To measure the demineralisation changes on human deciduous enamel produced by pH cycling after preventive treatment with Er:YAG laser irradiation, fluoride varnish application and a combination of the two. Sixty extracted human anterior primary teeth were assigned to six groups (n = 10). Group C: untreated; group L: Er:YAG laser; group TCP-NaF: Clinpro White (5% sodium fluoride and modified tricalcium phosphate); group CPP-ACP-NaF: MI varnish (5% sodium fluoride with casein phosphopeptide-amorphous and calcium phosphate); group L+TCP-NaF: Er:YAG + 5% sodium fluoride + modified tricalcium phosphate; group L+ CPP-ACP-NaF: Er:YAG + 5% sodium fluoride with casein phosphopeptide-amorphous and calcium phosphate. The samples were subjected to a 10-day pH-cycling regimen to create caries-like lesions, with 8 h in demineralising solution and 16 h in remineralising solution at 37°C. Enamel demineralisation was evaluated by laser fluorescence (DIAGNOdent) before and after pH cycling. The Mann-Whitney U-test and Wilcoxon tests were performed with statistical significance set at p ≤ 0.05. The Wilcoxon test revealed statistically significant differences at baseline and after pH cycling in groups C (p = 0.02), L (p = 0.034) and L+TCP-NaF (p = 0.025) and the lowest percentage of healthy tooth substance compared to the other groups. The results suggest that the treatment protocols employed in groups TCP-NaF, CPP-ACP-NaF, and L+ CPP-ACP-NaF had similar effects in terms of preventing demineralisation, as reflected in a higher percentage of healthy dental structure maintained. Hence, these treatments are recommended for clinical use as an effective preventive measure.

Clinical Comparative Evaluation of Nd:YAG Laser and a New Varnish Containing Casein Phosphopeptides‐Amorphous Calcium Phosphate for the Treatment of Dentin Hypersensitivity: A Prospective Study

The aim of this study was to compare the effect of Nd:YAG laser to that of a new varnish: MI Varnish with RECALDENT (GC), for the treatment of dentin hypersensitivity, with a follow-up of 6months. Dentinal hypersensitivity was evaluated using a new thermal test never before used on dental tissue. A split-mouth design was used where teeth on one side received the same type of treatment. Twelve patients suffering from dentin hypersensitivity were chosen. Fifty-four teeth were divided into 27 pairs. Twenty-seven teeth received the Nd:YAG laser treatment, and the 27 contralateral teeth received MI Varnish application. Five parameters were measured, and the measurements were taken before treatment at baseline (T0), after 1week (T1), 1month (T2), 3months (T3), and 6months (T4) of the application. Air stimulation to obtain the Schiff air score, the score of discomfort according to the visual analog scale (VAS), the tactile score, and the thermal test that determines the minimum cold-stimulating temperature and the maximum heat-stimulating temperature were all used to assess sensitivity. Statistical analyses of the data were performed, and the significance level was set at p ≤ 0.05. Exact Fisher tests and Chi-Square tests were conducted to compare percentages. There was a clear decrease in dentin hypersensitivity for both treatments (Nd:YAG laser and MI Varnish) especially between baseline and 1week, with maintenance of this state through the 6-month follow-up period. The difference between the two treatments was not significant, comparing, over time, the Schiff air score, the thermal test, and the VAS; however, the tactile score was significantly improved 6months after the application of MI Varnish compared to the laser (p=0.05). There was no significant difference between the two treatments, Nd:YAG laser and MI Varnish. Both treatments were effective and reduced dentin hypersensitivity immediately after treatment up to 6months.

Comparison of remineralization by fluoride varnishes with and without casein phosphopeptide amorphous calcium phosphate in primary teeth

Objective: To compare MI (5% NaF with 2% CPP-ACP) and Prevident (5% NaF) varnishes in remineralizing caries-like lesions in primary teeth regarding calcium and phosphate enamel content and lesion depth.Material and methods: Caries-like lesions were created in 48 primary teeth which were divided into 2 halves; one left untreated (control) and the other half treated with MI or Prevident varnishes. Calcium and phosphate content was assessed using energy dispersive X-ray spectrometer and reduction in lesion depth was assessed using polarized light microscopy. Demineralization and remineralization values in each group were compared using paired t test and percentage change between groups was compared using t test and Mann Whitney U test.Results: A greater percentage increase of calcium was observed in MI than Prevident specimens (median = 8.97 and 2.67, p < .0001), with greater calcium phosphate ratio percentage increase (median = 28.96 and 7.40) and phosphate percentage reduction (median = 15.5 and 4.51). The mean (SD) percentages reduction in lesion depth in the MI varnish was significantly greater than in Prevident varnish (44.41 (7.12) and 22.73 (9.35), p < .0001).Conclusions: MI varnish had better remineralization effect in primary teeth than Prevident varnish in terms of higher mineral content and shallower lesion depth.

Increasing the efficiency of CPP-ACP to remineralize enamel white spot lesions

ObjectivesTo compare the remineralization efficacy of using the MI paste plus according to manufacturer's instructions to MI varnish and to using a modified method of MI-paste plus application. Materials and Methods100 enamel specimens were obtained from the buccal and lingual surfaces of 50 extracted human non-caries third molars. All specimens were challenged by a buffered demineralization solution for 4 days, and were divided in 4 groups with 25 specimens in each group. 25 demineralized specimens had MI paste plus applied for 4 min and then wiped out (MI), 25 specimens had MI paste applied followed by application of SE-bonding agent (MI + Bond), 25 specimens had MI Varnish applied according to manufacturer instructions (MI Varnish) the rest of specimens served as controls (C). All specimens were stored for 7 days in artificial saliva. All specimens had their surface hardness (SH) measured by micro-hardness tester before/after the acidic challenge and after the treatment procedures. After the SH test all specimens were crosssectioned to obtain 100–150 micron thickness specimens to observe the lesion depth before/after treatment by the TMR (Transverse Micro Radiography) technique. ResultsTMR experiment showed that (MI + Bond) and (MI varnish) groups recorded significant decrease in lesion depth and mineral loss of the tested subsurface lesion p < 0.05. (MI + Bond) group scored the highest significant regain of surface micro hardness results p < 0.05. Conclusion: (MI varnish) and the modified application of MI paste are methods that can increase the efficacy of CPP-ACP in remineralizing the enamel surface lesions.

In vitro Comparison of Antimicrobial Activity of Conventional Fluoride Varnishes Containing Xylitol and Casein Phosphopeptide-Amorphous Calcium Phosphate.

Objective:Different fluoride varnishes are used for the prevention of dental caries. The aim of this study was to compare the antimicrobial effect of different fluoride varnishes, containing xylitol and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), on Streptococcus mutans.Materials and Methods:In this in vitro study, the antibacterial effects of four varnishes, namely Polimo and V-varnish (containing xylitol), MI varnish (containing CPP-ACP), and Preventa, were evaluated against S. mutans. The disc diffusion method was used for testing the bacterial sensitivity. The data were analyzed using the Kruskal–Wallis and Mann–Whitney U-tests.Results:According to the results, Polimo showed the highest antibacterial effects, compared to the other three varnishes (P ≤ 0.05). Growth inhibition zones were not observed in V-varnish and Preventa. The mean diameter of inhibition zone around the MI varnish was significantly higher, compared to those of the V varnish and Preventa (P ≤ 0.05).Conclusion:As the findings indicated, the fluoride varnish containing xylitol and CPP-ACP could be more effective iAQ1n the prevention of dental caries.

Remineralization potential of varnish containing casein phosphopeptides-amorphous calcium phosphate with fluoride and varnish containing only fluoride: A comparative study

Background: Fluoride varnishes have been the standard of practice for the professional application of fluoride. The primary reason for wide acceptance of fluoride varnish is that the procedure is easy, safe, convenient, and well-accepted by patients. Aim: The aim of this study is to evaluate and compare the remineralizing potential of varnish containing casein phosphopeptides-amorphous calcium phosphate (CPP-ACP) and fluoride (MI Varnish®), with that of varnish containing only fluoride (Fluor Protector®). Materials and Methods: Fifty premolar teeth samples were used for this part of the study. They were divided into two Groups (1 and 2), consisting of 25 samples each. Two windows on the buccal surface of these samples were made and assigned randomly as control and experimental window. Initial depth of carious lesions of both windows was measured in all the samples. No varnish was applied on control windows. On the experimental window of Subgroup 1, varnish containing CPP-ACP with fluoride was applied, and on samples of Group 2, varnish containing only fluoride was applied. After varnish application, all the samples were subjected to pH-cycling. Following, the final depth of carious lesion was calculated using polarized light microscope. The data obtained were tabulated and subjected to statistical analysis. Results: The final depths of artificial carious lesion of experimental window of Group 1 and 2 were 131.26 ± 7.01 μm and 134.11 ± 5.95 μm, respectively. Conclusion: There was no significant difference in the remineralizing potential of varnish containing CPP-ACP and fluoride and varnish containing only fluoride.

Comparative evaluation of calcium phosphate-based varnish and resin-modified glass ionomer-based varnish in reducing dentinal hypersensitivity: A randomized controlled clinical trial.

Objective:The aim of this study was to evaluate the efficacy of MI varnish and Clinpro XT varnish in reducing dentinal hypersensitivity.Materials and Methods:Patients with cervical dentinal hypersensitivity were selected for the study. The teeth to be tested were isolated. Then, a blast of air and ice cold water was applied on the tooth surface, and the score was measured by visual analog scale. The patients were randomly assigned to one of the treatment groups (Group 1: MI varnish; Group 2; Clinpro XT varnish). The manufacturer's instructions were followed. The sensitivity scores were recorded immediately and after 1 week of therapy.Statistical Analysis:Mann–Whitney U-test and Wilcoxon-matched pairs test were used for the analysis.Results and Conclusion:Although both varnishes were shown to reduce the dentinal hypersensitivity in patients, according to statistics, MI Varnish was a better agent to reduce dentinal hypersensitivity than Clinpro XT varnish.

Comparison of the Effect of Fluoride Varnish, Chlorhexidine Varnish and Casein Phosphopeptide- Amorphous Calcium Phosphate (CPP-ACP) Varnish on Salivary Streptococcus mutans Level: A Six Month Clinical Study.

Dental caries is the most widespread disease which has a multi factorial origin. Streptococcus mutans is considered to be the chief pathogen in its development. Different varnishes are available which can be used for the prevention of the carious process. To compare the effect of fluoride varnish, chlorhexidine varnish and fluoride varnish containing Casein Phosphopeptide - Amorphous Calcium Phosphate (CPP-ACP) on salivary Streptococcusmutans count in children with mixed dentition over a period of six months. Seventy two children of age 6-12 years with mixed dentition were selected fulfilling inclusion and exclusion criteria and divided into three groups: Group I- Fluoride varnish group; Group II- Chlorhexidine varnish group; Group III- MI varnish (fluoride varnish containing CPP-ACP) group. After thorough oral prophylaxis and restorative treatment the varnishes were applied to the teeth once a week for four consecutive weeks in the respective groups. Salivary Streptococcus mutans count (CFU/ml of saliva) was estimated at baseline, 1 month, 3 months and 6 months after varnish application. Bonferroni post-hoc test and paired t-test for inter group and intra group comparison was used in the study. A statistically significant reduction in salivary S. mutans colony count was seen with all three groups at all the different time intervals. Maximum reduction was noted in chlorhexidine varnish group at all the intervals after varnish application. Prevention of dental caries plays a vital role in paediatric dental practice. The use of dental varnish has proved to be effective in reducing the dental caries. Chlorhexidine varnish was found to show maximum reduction in salivary S. mutans count for six month period, when compared to MI varnish and fluoride varnish. Thus, chlorhexidine varnish can be used as potent caries inhibiting agent and promote good oral health.

Effect of Fluoride Varnish Containing CPP-ACP on Preventing Enamel Erosion.

This study aimed to investigate the effect of a fluoride varnish with added casein phosphopeptide-amorphous calcium phosphate treatment on the prevention of enamel erosion, and it compared the results with those of other fluoride varnishes. Fifty enamel specimens obtained from bovine incisors were randomly divided into five groups (n = 10) based on the type of surface pretreatment used: intact enamel (Group 1); intact enamel+erosive cycles (Group 2); intact enamel+MI varnish+erosive cycles (Group 3); intact enamel+Clinpro White varnish+erosive cycles (Group 4); and intact enamel+Duraphat varnish+erosive cycles (Group 5). The specimens were subjected to erosive cycles for five days. The surface roughness was evaluated using atomic force microscopy. The results were statistically analyzed using one-way ANOVA and Tukey's tests. Group 1 had the smoothest surfaces. After the erosive cycles, the greatest surface roughness values were observed in Group 2, followed by Groups 5, 4, and 3, respectively. Statistically significant differences were observed among all groups (p < 0.05). The application of fluoride varnishes had some positive effects on preventing enamel erosion; however, the most effective agent was fluoride varnish with added casein phosphopeptide-amorphous calcium phosphate.

Effect of fluoride varnish with added casein phosphopeptide-amorphous calcium phosphate on the acid resistance of the primary enamel.

BackgroundThis study aimed to investigate the effects of a fluoride varnish with added Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) treatments on acid resistance of primary teeth enamel.MethodsEnamel specimens obtained from 40 primary incisors (for surface microhardness testing) and 40 primary molars (for demineralization depth measurement) were randomly divided into four groups (n = 10 incisors and 10 molars) each according to surface treatment: no treatment (control), MI varnish (1–8 % sodium fluoride and 1–5 % CPP-ACP), Clinpro White (1–5 % sodium fluoride and <5 % modified tricalcium phosphate), Duraphat (<5 % sodium fluoride). Specimens were stored for 24 h in a moist environment. After varnish residues were removed, specimens were subjected to pH cycling. The effects of fluoride varnishes were evaluated according to surface microhardness, lesion depth and structural changes. Results were analyzed by ANOVA and Tukey’s tests.ResultsThe lowest changes in surface microhardness and lesion depth occurred in MI varnish group, followed by the Clinpro White, Duraphat and no treatment (control) group (for percentage of loss surface microhardness −20.80, −34.60, −57.80 and −73.40; for lesion depth values 23.60 μm ± 3.36, 29.85 μm ± 3.27, 40.37 μm ± 3.41 and 54.56 μm ± 4.16, respectively). Statistically significant differences in both surface microhardness and lesion depth were observed among all groups (P < 0.05).ConclusionsWithin the limitations of this in vitro study, fluoride varnish containing CPP-ACP was more effective in increasing the acid resistance of primary enamel than other fluoride varnishes. However, further clinical research is needed to confirm these in vitro results.

Effect of fluoride varnish with added casein phosphopeptide-amorphous calcium phosphate on bond strength to enamel

To investigate the effect of fluoride varnish with added casein phosphopeptide-amorphous calcium phosphate on the shear bond strength (SBS) of two adhesive systems to enamel. Specimens obtained from permanent teeth were randomly distributed among four groups for enamel pretreatment [Control (no treatment, CNT), Duraphat varnish (DV), Clinpro White varnish (CWV), MI Varnish (MIV)], and each group was further divided into two subgroups according to adhesive [Etch&rinse (Adper Single Bond, ASB), self-etch (Clearfil SE Bond, CSE)]. Specimens were stored in distilled water at 37 °C for 24 h. Cylindrical composite specimens (2.3 mm in diameter, 3.0 mm in height) were then bonded to the enamel surfaces. SBS tests were performed and data were analyzed with two-way ANOVA and Tukey’s tests. For both CSE and ASB, SBS values of the CNT groups were significantly higher than those of all the enamel pretreatment groups (p < 0.05). Among the enamel pretreatment groups, SBS values with both adhesive systems were lowest in the MIV groups, followed by CWV and DV groups. In conclusion, pretreatment of enamel surfaces with fluoride-containing varnishes reduced bonding performance of adhesive systems to enamel. MIV appeared to cause greater enamel surface alterations and precipitation, which interfered with adhesive bonding mechanisms.