Healing Methods Research Articles

Study of structural-mechanical properties for choosing the basis of pharmaceutical composition in ointment form

Rational treatment of wounds is a pressing problem of our time, especially during hostilities. Practice has shown that currently there is no universal method of local wound healing. Therefore, a differentiated approach to the development of drugs for the local treatment of the wound process is needed. Among the dosage forms for topical use, soft drugs are a convenient dosage form for topical use. Thanks to the excipients which are a part of soft medicines, it is possible to regulate release of active pharmaceutical ingredients that, in turn, provides medical action of drug. The technology of drug preparation, which includes the substantiation of the temperature regime of drug production, the study of their physico-chemical and structural-mechanical properties affects the therapeutic efficacy of the drug.
 The aim of the work was to study the structural and mechanical properties of model samples for choosing the basis of the pharmaceutical composition in the form of an ointment.
 The object of the study were model samples of bases made on the basis of oil/water emulsion and water/oil. Measurements of rheological parameters of ointment bases and their homogeneity were performed according to the methods of the State Pharmacopoeia of Ukraine (2.2.10 and SPU 1 in Annex I).
 Studies of thermal stability and colloidal stability were performed according to DSTU 4765: 2007. Cosmetic creams. General technical conditions. [Effective from 2009-01-01]. Rheological studies of model samples of ointment bases immediately after their manufacture and their physicochemical properties (homogeneity, thermal stability and colloidal stability) both after fabrication and during 6 months of storage at room temperature. It is proved that all samples have elastic-plastic-viscous properties, thixotropy and ability to lubricate well, homogeneity and thermal and colloidal stability.
 The study of the structural and mechanical properties of model bases has shown that these samples of bases can be used as carriers of drugs in the development of soft drugs.
 The prospect of this study is to study the osmotic properties of model samples in order to choose the basis that the medical and biological requirements will meet a certain phase of the wound process.

THE REVIVAL OF ISLAMIC TRADITIONAL MEDICAL METHOD AMONG MUSLIM SOCIETY DURING PANDEMIC

The Corona Virus Pandemic not only affects the social life of the community but also encourages many Muslim communities to find alternative solutions to maintain the vitality of their bodies. Consuming herbal medicines and conducting some Islamic traditional medical methods are currently back into a social trend because some people believe that the methods can ward off all forms of the disease. The focus of this research is the implementation of traditional Islamic medicine methods in West Java and aims to understand the impact of the use of alternative medical methods in society in depth. This study used a descriptive qualitative method with a field analysis approach using observation and interview techniques to collect the required data. The findings obtained by the author also shows that the use of herbal medicine and traditional Islamic healing methods can have a big impact on beliefs related to the function of religion in protecting people in the world and the hereafter and they also have the belief that the activities they do increase their immune system. This is because the theological conception of religious-based medicine is increasing being preached by many religious figures and medical personnel

Doa dan Wirid Al-A’la Sebagai Metode Sufi Healing (Praktek Batatamba Guru Arni)

<p class="07KatakunciKeywords">Sufi Healing is an alternative medicine for the community in overcoming physical, psychological and magical diseases. Belief in magic that causes physical and psychological problems in a person is still a strong belief for the Banjar people. The <em>Wirid Al-A'la</em> prayer as one of the Sufi healing methods is used by Guru Arni to overcome this problem. The collaboration of the <em>Batamba</em> and Sufistic Guru Arni methods is a unique Sufi healing method. The findings of the data through interviews and observations from deeper exploration of Sufi healing will be analysed descriptively. The results showed that the people of Banjar make prayer and <em>wirid Al-A'la</em> as an alternative medicine because of the integration between <em>Batamba</em> medicine and Sufism. Healing from magical diseases is claimed by the people of Banjar thanks to the collaborative method used by Guru Arni. <em>Batamba</em> is a treatment by prioritizing the values of local wisdom while Sufism is a Sufism approach used in the treatment process. These findings confirm that using a cultural approach in the treatment process can help the community in overcoming a number of problems. The Sufistic nuance is deepened so that people are not trapped in matters of faith.</p>

Design and Development of Neomycin Sulfate Gel Loaded with Solid Lipid Nanoparticles for Buccal Mucosal Wound Healing.

Drug administration to the wound site is a potential method for wound healing. The drug retention duration should be extended, and drug permeability through the buccal mucosal layer should be regulated. Oral wounds can be caused by inflammation, ulcers, trauma, or pathological lesions; if these wounds are not treated properly, they can lead to pain, infection, and subsequent undesirable scarring. This study aimed to develop Kolliphor-407 P-based gel containing neomycin sulfate (NES) loaded in solid lipid nanoparticles (SLNs) and enhance the antimicrobial activity. By considering lipid concentrations and achieving the lowest particle size (Y1) and maximum entrapment (EE-Y2) effectiveness, the formulation of NES-SLN was optimized using the Box–Behnken design. For the selected responses, 17 runs were formulated (as anticipated by the Design-Expert software) and evaluated accordingly. The optimized formulation could achieve a particle size of 196.25 and EE of 89.27% and was further utilized to prepare the gel formulation. The NES-SLN-G formula was discovered to have a smooth, homogeneous structure and good mechanical and rheological properties. After 24 h of treatment, NES-SLN-G showed a regulated in vitro drug release pattern, excellent ex vivo permeability, and increased in vitro antibacterial activity. These findings indicate the potential application of NES-SLN-loaded gels as a promising formulation for buccal mucosal wound healing.

Diatomite and Na-X zeolite as carriers for bacteria in self-healing cementitious mortars

The use of bacteria in the self-healing process of cementitious composites has been recently widely considered. In this study, diatomite and the Na-X zeolite were proposed as carrier materials impregnated by the Bacillus subtilis endospores. The effect of the impregnation method on a bacterial survival rate (BSR) and cement composites modification method on the strength parameters, was investigated. Additionally, the influence of modification and healing method on the flexural and compressive strength recovery and surface crack repair ratio, was assessed. Test results indicated higher BSR for a vacuum impregnated than immersed samples (up to 31% after 28 days of storage for the impregnated Na-X zeolite). The BSR in the self-healing mortar may be promoted by local pH reduction in the vicinity of carrier particles, especially diatomite (pHPZC = 4.6). Mortars containing medium dissolved in the mixing water showed an increase in the flexural and compressive strength (up to 10.4% and 18.0%, respectively). The addition of bacteria immobilized on the both carrier materials caused a decrease of strength properties (to 16.6%), while the series containing both bacteria and the medium attained the strength properties similar or better than the reference series and the series with carrier materials alone. Series containing bacteria immobilized on carrier material and with medium showed a self-healing ability, which was confirmed by the compressive strength recovery and surface repair ratio of 32.6% and 92.6%, respectively. The increased precipitation of CaCO3 was confirmed by the SEM and EDS analysis.

Healing by Spiritual Possession in Medieval Japan, with a Translation of the Genja sahō

From the mid-10th century onward, in cases of illness, the Japanese aristocracy relied on new Buddhist healing methods based on spiritual possession techniques. This essay examines the features and procedures according to which monks and mediums operated the healing. This method, of Indian origin, was imported in Japan through Esoteric Buddhism, and was adapted in order to fit healing purposes. The author focuses his analysis on the role played by an invisible “spirit” who acted to catch the ill-causing demon within the patient’s body and expelled this malign entity from it. The article ends with a translation of a unique ritual text entitled Genja sahō 驗者作法, which describes these rituals in detail.

Glucose/ROS cascade-responsive ceria nanozymes for diabetic wound healing.

Diabetic wounds have an extremely complex microenvironment of hyperglycemia, hypoxia and high reactive oxygen species (ROS). Therefore, the regulation and management of this microenvironment may provide a new and improved treatment method for chronic diabetic wound healing. Herein, a glucose/ROS cascade-responsive nanozyme (CHA@GOx) was developed for diabetic wound treatment based on Ce-driven coassembly by a special dual ligand (alendronic acid and 2-methylimidazole) and glucose oxidase (GOx). It possesses superoxide dismutase and catalase mimic activities, which effectively remove excess ROS. In particular, it can catalyze excessive hydrogen peroxide generated by the glucose oxidation reaction to produce oxygen, regulate the oxygen balance of the wound, and reduce the toxic side effects of GOx, thus achieving the purpose of synergistically repairing diabetic wounds. In vitro experiments show that CHA@GOx assists mouse fibroblast migration and promotes human umbilical vein endothelial cell tube formation. In vivo, it can induce angiogenesis, collagen deposition, and re-epithelialization during wound healing in diabetic mice. Taken together, this study indicates that the coassembly of multifunctional nanozymes has implications in diabetic wound healing.

A Review on Homa Farming – A Vedic Touch to Modern Agriculture

Homa farming is a Vedic discipline which denotes the method of annihilating harmful circumstances of environmental elements and refines the atmosphere by action of flame, outfitted with copper pyramid. Agnihotra is essential flame in Homa farming. It religiously associates living beings on this earth to control energy from space. It is practiced when all hope is gone and has proved to be beneficial in increasing yield of crop , reducing microbial pathogenicity , decontaminating soil and water, against pest and disease infestation. Homa farming is comprehensive method of healing of agriculture and can be used in conjunction with any good organic farming system as it is extremely inexpensive and can be performed by anybody but requires discipline and consistency. In course of time this knowledge has lost because the farming is becoming more modernized with invention of new technologies like GI , GPS , satellite imaging, moisture sensors. Farmers are approaching new methods and practices of farming and they completely relies on chemicals like pesticides, rodenticides, Fertilizers, herbicides to enhance his production. Then it becomes difficult for them to believe in traditional and Vedic type of agriculture as it purely organic in nature and only depends on healing effects of agnihotra. Nowadays this knowledge is being revived by many scientists to give individuals the guidance about how to address polluted conditions of planet. Many scientists have demonstrated the scientific validation of Homa farming methodologies and have conducted experiments to prove the beneficial effect of Homafarming. A famous Scientist named Abhang Pathade, has conducted many experiments to prove that this technique actually works and can be very effective in getting rid of major problems like environmental pollution , disease and pest attack on plants , less crop yield and soil infertility.

COL11A1 is Downregulated by miR-339-5p and Promotes Colon Carcinoma Progression.

The roles of COL11A1 in cancer have been increasingly considered, but the understandings of the effects of COL11A1 on colon carcinoma progress are much limited yet. qRT-PCR and Western blot were utilized to evaluate COL11A1 expression at mRNA and protein levels, respectively, in colon carcinoma cell lines. Afterward, the tumorigenesis biological effects of COL11A1 were examined by CCK-8, colony formation, Transwell, and wound healing methods. Moreover, upstream miRNAs containing the binding sites with COL11A1 were predicted by the bioinformatics methods. The interplay between COL11A1 and miR-339-5p was identified by a dual-luciferase assay. COL11A1 expression was prominently upregulated in colon carcinoma cell lines relative to that in normal human colon mucosal epithelial cell lines, and it was related to tumor stages. The outcomes of in-vitro experiments suggested that interfering with COL11A1 remarkably repressed the malignant behaviors of SW480 and SW620 cells. MiR-339-5p was markedly lowly expressed in colon carcinoma cell lines. Furthermore, miR-339-5p directly targeted and negatively regulated COL11A1 expression. COL11A1 upregulation promoted colon carcinoma cell functions, while overexpressing miR-339-5p evidently attenuated the promotion. These results proved the modulation of the miR-339-5p/COL11A1 axis in colon carcinoma cells, and miR-339-5p repressed colon carcinoma progression via COL11A1 downregulation. These results offer new underlying targets for the accurate therapy of colon carcinoma patients.

Modern Wound Treatment with Moist Wound Healing Effective for Healing Diabetic Ulcus

Diabetes Mellitus is a metabolic disease that is chronic and requires ongoing medical care in a variety of ways that can reduce the risk of multi-factors beyond glycemic control. Uncontrolled diabetes mellitus will cause an increase in complications, one of which is diabetic ulcers. Achieving better wound healing is the main goal of diabetic ulcer management. Moisturizing dressings can provide a supportive environment for cells to carry out the wound healing process and prevent further damage or trauma. This study was to determine the effect of modern dressing with moist healing on diabetic ulcers. This type of research is a quantitative research with a quasi-experimental research design with an accidental sampling approach. Data was collected using demographic data and the Wocare For Indonesian Nurses (WINNERS) Scale assessment sheet which is a modified Bates-Jasen score to predict and evaluate the average score of wound healing. WINNERS scale consists of 10 assessments. The results of the intervention that have been carried out have shown a significant improvement in diabetic ulcers as assessed using the Bates Jensen Wound Assessment Tools. Based on the results and discussion, it was concluded that there was a significant effect of modern wound care using the moist wound healing method on the healing process of diabetic ulcers.

Comparison of Clinically Relevant Adipose Preparations on Articular Chondrocyte Phenotype in a Novel In Vitro Co-Culture Model.

Adipose therapeutics, including isolated cell fractions and tissue emulsifications, have been explored for osteoarthritis (OA) treatment; however, the optimal preparation method and bioactive tissue component for healing has yet to be determined. This in vitro study compared the effects of adipose preparations on cultured knee chondrocytes. De-identified human articular chondrocytes were co-cultured with adipose preparations for 36 or 72 h. Human adipose tissues were obtained from abdominal panniculectomy procedures and processed using three different techniques: enzymatic digestion to release stromal vascular fraction (SVF), emulsification with luer-to-luer transfer (nanofat), and processing in a bead-mill (Lipogems, Lipogems International SpA, Milan, Italy). Gene expression in both chondrocytes and adipose preparations was measured to assess cellular inflammation, catabolism, and anabolism. Results demonstrated that chondrocytes cultured with SVF consistently showed increased inflammatory and catabolic gene expression compared with control chondrocytes at both 36- and 72-h timepoints. Alternatively, chondrocytes co-cultured with either nanofat or bead-mill processed adipose derivatives yielded minimal pro-inflammatory effects and instead increased anabolism and regeneration of cartilage extracellular matrix. Interestingly, nanofat preparations induced transient matrix anabolism while Lipogems adipose consistently demonstrated increased matrix synthesis at both study timepoints after co-culture. This evaluation of the regenerative potential of adipose-derived preparations as a clinical tool for knee OA treatment suggests that mechanically processed preparations may be more efficacious than an isolated SVF cell preparation.

Comprehensive Self-Healing Evaluation of Asphalt Concrete Containing Encapsulated Rejuvenator.

Ultraviolet radiation, oxidation, temperature, moisture, and traffic loads produce degradation and brittleness in the asphalt pavement. Microcracks develop into macrocracks, which eventually lead to pavement failure. Although asphalt has an inherent capacity for self-healing, it is constricted. As a result, damages build beyond the ability of asphalt to repair themselves. This research employs the in-situ crack healing method of encapsulated rejuvenator technology to enhance the insufficient self-healing capability of roads. This allows the extrinsically induced healing in asphalt to assist it in recovering from damage sustained during service life. Optical microscopy, thermogravimetric analysis, and the compressive load test of capsules were done to characterise their properties. We measured the self-healing behaviour of encapsulated rejuvenator-induced asphalt utilising the three-point bending beam tests on unaged, short-term aged and long-term aged asphalt beams. The rate of oil release before and after healing was quantified using Fourier transform infrared spectroscopy. The results of these tests were utilised to explain the link between healing time, temperature, asphalt ageing, and healing level. Overall, it was determined that the encapsulated rejuvenator was acceptable for mending asphalt mixes because it increased healing temperature and duration, resulting in an up to 80% healing index.

C1QTNF6 Targeted by MiR-184 Regulates the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells.

To seek out the mechanism by which C1QTNF6 mediates lung adenocarcinoma (LUAD). Differentially expressed mRNAs and miRNAs in LUAD were analyzed using bioinformatics. In LUAD cells, C1QTNF6 mRNA and miR-184 expression were evaluated with qRT-PCR, and C1QTNF6 protein level was assessed by western blot. Cellular behaviors were assessed by colony formation, CCK-8, Transwell, and wound healing methods. The binding ability of miR-184 to C1QTNF6 was observed by dual-luciferase assay. High expression of C1QTNF6 in LUAD stimulated cancer cellular behaviors. MiR-184 was lowly expressed in LUAD and downregulated C1QTNF6 expression. MiR-184 restrained LUAD cell processes by targeting C1QTNF6. MiR-184 repressed LUAD cell processes via mediating C1QTNF6. MiR-184 and C1QTNF6 are expected to be indicators for LUAD treatment.

Effect of Vitamin D Supplementation on Fracture Healing: A Systematic Review of Prospective Clinical Trials

Background: The relationship of vitamin D status with regard to its deficiency and supplementation in the setting of fracture healing has not been well established. This review aims to evaluate the efficacy of vitamin D on clinical fracture healing Methods: A systematic review of English articles using EMBASE, MEDLINE and the Cochrane Controlled Trials Register was performed. Search terms included vitamin D, cholecalciferol, colecalciferol, hydroxycholecalciferol, calcifediol, calcitriol, dihydroxycholecalciferol, ergocalciferol, dihydrotachysterol, viosterol, 1,25- hydroxyvitamin D, 25-hydroxyvitamin D, 24,25-dihyroxyvitamin D, 25 hydroxyvitamin D, lunacalcipol, vitamin D3 and fracture healing or repair. Only prospective, original trials investigating vitamin D deficiency or its supplementation on fracture related outcomes in humans were included. Results: Overall rates of delayed union in the setting of vitamin D deficiency are low. Vitamin D supplementation appears to have no effect on eventual fracture union in an adult fracture population when assessed by clinical examination and plain radiographs. Conclusions: Prospective, interventional studies of vitamin D supplementation on fracture healing are yet to demonstrate an effect on fracture healing outcome measures.

ANALISIS SEMIOTIKA KOMUNIKASI PADA BARONG BRUTUK DI DESA TRUNYAN KINTAMANI BANGLI

Barong Brutuk is an ancient sacred art that our ancestors passed down way before Hinduism was introduced to Indonesia. It is still practiced and observed today by the people of Trunyan Village in Kintamani Bangli. This research is conducted in order to know (1) the symbols that used in Barong Brutuk, (2) the communication function of Barong Brutuk, (3) the meaning of communication semiotic from sender to the receiver of message. This research is a qualitative research, that using the theory of communication semiotic, theory of religious communication, and theory symbolic interaction. Analytical techniques with hermeneutic analysis. Based on the results of the analysis and discussion, it came to our conclusion that the symbols used in Barong Brutuk in Trunyan Village consist of mask (tapel), kraras leaves, and whip. The mask symbolizes the meeting of the god Trunyan with his future consort, it is interpreted as the meeting of man and women led to believe to be the start of human life. The kraras leaves symbolizes the soil fertility, protecting the environment and the prevention of natural disasters. The belief of self-sacrifice as a means of healing disease, the hope of obtaining offspring is symbolized by the whip. Barong Brutuk has many purposes and uses such as communication, as a means of pleasure, and entertainment for the community, it also reflects on the Trunyan Villages identity as an integrative tool. It’s a method of healing, education and integration during a time of chaos.

Concepts and Principles of Contemporary Hypno-Psychotherapy

Hypnosis is empirically well studied in its biological foundations and its therapeutic effectiveness. It is a recognised healing method in Austria, Switzerland and Germany. With the use of imaging techniques, it can be clearly shown that brain areas responsible for attention, visual imagination, critical evaluation and self-awareness change in their activity through hypnosis in such a way that the state of hypnotic trance differs markedly from both sleep and waking consciousness. The effects of hypnosis on the immune system as well as on affects have been confirmed. Basically, a fundamental distinction must be made between “therapeutic hypnosis”, hypnosis for therapeutic purposes, and “show hypnosis”, such as is shown in various entertainment performances. Therapeutic hypnosis” is in turn divided into different forms, namely “hypnotic psychotherapy”, “hypnotherapy” and “hypnosis”.

Traditional oral healthcare practices followed by the Garo tribe of West Garo Hills District, Meghalaya

Ethnomedicinal study of therapeutic healing methods of oral diseases was conducted during 2016-19 in the West Garo Hills district of Meghalaya, India. Recurrent field trips in and around different parts of West Garo Hills lead to the five most knowledgeable and available THPs (Traditional health practitioners) from whom valuable information was elicited. A pre-tested interview schedule was used to gather information from them. Information like vernacular names, common names, scientific names, family, habit, habitat, plant parts used and a comprehensive procedure for preparation of the medicines was recorded. After obtaining detailed information regarding the indigenous wild medicinal plants used wholly for healing oral diseases, an assessment was made that, 14 plant species belonging to 12 different families were found to heal the dreadful and painful disease. Out of the 14 plants having medicinal value, the most important plants used were Curcuma longa L., Piper nigrum L., Piper longum L. and Mimosa pudica L., etc. These 14 medicinal plant species are comprised of trees (five), shrubs (three), herbs (three), vines (two) and grass (one). Among the plant parts used, the leaves were the most primarily used plant part, followed by root and rhizome. The plants were either used alone or in combination with other species. The administration of these herbal medicines was mostly done orally in the form of a juice/decoction. In some cases, the juice was also used to rinse the mouth. These medicinal plants were mostly collected from nearby forests, local markets and kitchen gardens. The present paper aims for the documentation of the ever-diminishing information on the wisdom and knowledge of the Garo THPs on use of various herbs in the traditional oral healthcare system and the detailed description of the preparation and treatment procedure that were practiced by the Garo THPs in the West Garo Hills of Meghalaya.

Scientific Explanation of the Hawaiian Method of Healing and Life Success Hooponopono

The present article offers a scientific explanation of how the Hawaiian hooponopono method heals violent mentally sick patients from a distance. The name of the method is explained as “hoo” -“restore” and “ponopono”-“harmony”, which sums up to a way to restore the harmony. Cleansing the conscious mind until its activity drops down to zero allows reaching the subconscious mind, which restores the harmony. Since the Subconscious is the birthplace of our Emotional Mind and Emotional Spirit, the emotion of Love is sent to the violent mentally suck from a distance to restore their lost emotional harmony. Scientific research did found that meditation on Love made the waves of the brain and the heart more harmonic, and harmonic functioning means health. The article also explains why only by using subconscious guidance, i.e. by letting the Spirit of God in the Subconscious guide us, we can achieve success in life. It is also scientifically explained why beside cleansing of the conscious mind, energy cleansing of the lecture hall was necessary before the lecture on hooponopono method took place.

О традициях народной медицины аларских бурят

Introduction. The article provides a first insight into local and regional folk medicine traditions of Alar Buryats (once a part of Balagansk Buryats) nowadays inhabiting Alarsky District of Ust-Orda Buryat Autonomous Okrug (Irkutsk Oblast, Russia) and clustering with Buryats from the western coast of Lake Baikal, the latter therefore referred to as ‘western’ or ‘Cis-Baikalia’ (sometimes ‘northern’) Buryats. Healing practices of present-day Alar Buryats include richest experiences of previous generations, the former largely determined by a number of natural and geographic factors and conditions of the forest-steppe zone that resulted in consistent combinations of economic patterns, such as arable farming, semi-sedentary livestock breeding, seasonal hunting and fishing. Folk medicine of this sub-ethnic group retains strong ties to shamanic beliefs to have survived the long decades and centuries of persecution. The research topic proves interesting enough since the region under consideration — like the entire Baikal Region — is characterized by a lack of Tibetan medicine influence to have resulted from Orthodox Christianity’s resistance to the expansion of Buddhism. This factor made it possible to preserve the unique ancient traditional treatment techniques, while shamanic healing patterns based on a mythological worldview also constitute a large unexplored area of Baikalia at large. So, local healing traditions remain somewhat understudied. There are only fragmentary data in some works on this subject. Goals. The study aims to analyze the available data on traditional healing practices of Alar Buryats — publications, archival materials, and field notes. Results. The article classifies a set of animal and plant medicines, methods of psychological impact, irrational practices, preventive healthcare approaches and shamanic healing methods. Special attention is paid to etymologies of names of certain diseases and dialect medical terms. An effort is made to analyze the nature of some diseases and phenomena in comparison to healing traditions of other ethnic groups with due regard of modern medical knowledge.

Successful application of conditioned culture medium for the treatment of a chronic wound of an amputation stump: a clinical case

Background: Amputation of the lower extremities is a necessary procedure to save a patient with critical arterial and neurotrophic disorders in the lower extremities. The amputation stump-related complications develop in many patients with diabetes mellitus (up to 40% of the total population).
 Clinical case description: Patient Yu., 64 years old, was admitted on October 19, 21 for an outpatient treatment of purulent-necrotic wounds of the amputation stump of the right lower limb. А high amputation was performed on September 24, 2021 due to thrombosis of the femoral-tibial bypass, installed on September 08, 2021 (bypassing below the knee joint gap with a Vascutek 7 mm synthetic prosthesis on the right) and the development of critical ischemia of the right lower limb with necrosis of the distal phalanges of the right foot toes. The wound was assessed according to the Bates-Jensen scale (BJ) and examined according to the developed protocol. The wound treatment was carried out according to an individual plan using a conditioned culture medium from mesenchymal stem cells (CM-MSCs), which stimulates angiogenesis and improves remodeling and recovery in the wound area. CM-MSC application made it possible to reduce the healing time and achieve a scarless closure of the tissue defect.
 Conclusion: The use of CM-MSC can be an effective method for healing a purulent-necrotic postoperative wound resulting from amputation of a limb in patients with critical ischemia of the lower extremities.