In order to develop an efficient micro heat exchanger and micro fuel cell, a number of studies of two phase flow such as liquid-gas in micro scale have been reported. In the micro scale, since surface tension became dominant, micro droplet or liquid film on a wall affects the dynamics of fluid. In the paper, in order to improve measurement accuracy of the three-dimensional shape measurement method for droplet based on a micro laser induced fluorescence technique, experimental conditions such as micro fabrication method, concentration of fluorescence dye, and intensity of excitation light were optimized. The technique was applied to a micro droplet on a wall resulting in achievement of high spatial and temporal resolution. The measurement accuracy of the present method was 0.57μm in comparison to a laser microscope. The result indicated that the present method is suitable for investigation of two phase flow in micro scale.