Method For Somatic Embryogenesis Research Articles

Somatic embryogenesis and Agrobacterium-mediated transformation of chrysanthemum

A method of somatic embryogenesis from leaf segments of Dendranthema grandiflora Tzvelev., cultivars ‘Yellow Spider’, ‘White Snowdon’, ‘Orange Westland’ and ‘Mistletoe’ was developed and used for transformation of these cultivars by Agrobacterium. Two different Agrobacterium tumefaciens strains were used: the wild type B6S3 and the disarmed strain carrying in its T-DNA the β-glucuronidase gene and the neomycine phosphotransferase II gene as a selectable marker gene. Non-chimeric transformed plants were obtained. The cultivar ‘White Snowdon’ has been proved to be susceptible to Agrobacterium transformation. Regenerated plants showed stable octopine synthase or β-glucuronidase (GUS) expression during vegetative propagation.