Method For Synthesis Of Nanoparticles Research Articles

Eco-friendly Synthesis of Silver Nanoparticles and its Application in Hydrogen Photogeneration and Nanoplasmonic Biosensing.

Environmentally friendly methods for silver nanoparticles (AgNPs) synthesis without the use of hazardous chemicals have recently drawn attention. In this work, AgNPs have been synthesized by microwave irradiation using only honey solutions or aqueous fresh pink radish extracts. The concentrations of honey, radish extract, AgNO3 and pH were varied. AgNPs presented mean sizes between 7.0 and 12.8 nm and were stable up to 120 days. The AgNPs were employed as co-catalyst (TiO2 @AgNPs) to increase the hydrogen photogeneration under UV-vis and only visible light irradiation, when compared to pristine TiO2 NPs. The prepared photocatalyst also showed hydrogen generation under visible light. Additionally, AgNPs were used to assemble a nanoplasmonic biosensor for the biodetection of extremely low concentrations of streptavidin, owing to its specific binding to biotin. It is shown here that green AgNPs are versatile nanomaterials, thus being potential candidates for hydrogen photogeneration and biosensing applications.

Comparison of different methods for synthesis of iron oxide nanoparticles and investigation of their cellular properties, and antioxidant potential

Iron oxide nanoparticles could play a useful role in lung cancer therapy. Iron oxide nanoparticles (NPs) were synthesized by plant mediated synthesis, chemical, and microbial mediated synthesis. iron oxide nanoparticle polyethylene glycol cis-diamminedichloroplatinum (Fe2O3@PEG@CDDP(, iron oxide nanoparticle polyethylene glycol (Fe2O3@PEG), and cis-diamminedichloroplatinum (CDDP) were evaluated for their antioxidant,and in vitro cytotoxicity tests. X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FE-SEM), mapping, and zeta potential were used to characterize the synthesized iron oxides NPs. Cell toxicity was determined using A549 and HFF cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The antioxidant scavenging activity of Fe2O3@PEG@CDDP, Fe2O3@PEG, and CDDP displayed IC50 values (11.96, 26.74, and 3.17 μg/ml) and (8.54, 11.4, and 1.14 μg/ml) in 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays, respectively. Nanoparticles obtained from plant mediated synthesis method showed the great antioxidant activity. Results showed that, green-method synthesized nanoparticles were the most effective at killing cancer cells. Thus, the characteristics of nanoparticles from green synthesis are more valuable than the other methods. Green synthesis is environmental friendly cost-effective, and easy approach for synthesize NPs.

Cymodocea serrulata-capped silver nanoparticles for battling human lung cancer, breast cancer, hepatic cancer: Optimization by full factorial design and in vitro cytotoxicity evaluation

In recent times, there has been growing interest in nanoparticles (NPs) synthesized through biological means due to their ease of production and their potential applications in the field of biology. This study presents an environmentally friendly method for the biogenic synthesis of silver nanoparticles (AgNPs) using the leaf extract of Cymodocea serrulata as both a reducing agent and a capping agent. Various physico-chemical and microscopic techniques were employed to comprehensively characterize the biogenically produced AgNPs. The results of these characterization studies confirmed the formation of spherical, stable, and crystalline AgNPs with an average size of 30.5 ± 2.5 nm. Furthermore, the antibacterial assessment revealed the remarkable antibacterial properties of these biogenically synthesized Ag NPs, even at exceedingly low concentrations ranging from 50 to 100 μg/mL. The IC50 values for the biogenically synthesized AgNPs against different human cancer cell lines, such as A549, MDA-MB-231, HepG2, and MCF-7, were determined to be 93.4 ± 4.5, 82.5 ± 3.7, 87.6 ± 4.1, and 57.3 ± 2.5 μg/mL, respectively. Most notably, the biogenically synthesized Ag NPs exhibited significant anti-inflammatory activity, as evidenced by their IC50 value of 30.08 ± 1.4 μg/mL, as assessed through the HRBC membrane stabilization method. These in vitro findings strongly suggest that AgNPs fabricated through biogenic processes using Cymodocea serrulata leaf extract hold promise as potential therapeutic candidates for combating bacterial infections, cancer, and inflammatory conditions.

Natural luminescent carbon nanoparticles extracted from coconut water

The synthesis methods of carbon nanoparticles (CNPs) are of utmost importance to the commercialization of CNPs as they need to have an efficient cost-to-gain. In this paper, we report on a facile method for extracting CNPs out of a renewable and green source of coconut water without the need for any time or energy consuming synthesis procedure. Namely, the CNPs were essentially extracted by filtration of coconut water using a dialysis bag to remove long-chain carbohydrates and bulk contaminants. The obtained CNPs were characterized using several techniques including high-resolution transmission electron microscope (HRTEM), photoluminescence (PL) spectroscopy, ultraviolet–visible (UV–Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), zeta potential analyzer, time-resolved photoluminescence lifetime measurement, and X-ray diffraction (XRD). It was found that the obtained CNPs have an average lateral size of 4.5 nm and interestingly, show UV and blue light emissions when excited optically by deep UV light. The latter indicates the potential application of these CNPs in optoelectronics. XPS data shows a C/O ratio of 65%/28% which is an expected result for the surface bonds of a CNPs and is in agreement with the FTIR data which demonstrates a dominance of carbon and oxygen-based function groups. The CNPs also demonstrate a long fluorescence lifetime of 5.3 ns which is relatively longer than that in literature.

Synthesis and Morphological Control of Fe Metal Particles By Controlling the Metal Complex Condition in Basic Solution

Coronavirus outbreaks have recently necessitated rapid virus recovery and vaccine developments. Magnetic beads are essential components to recover virus in an immunoprecipitation process. Pure iron particles with a diameter of 1 µm can be used as core materials of high-performance magnetic beads.Thus, the development of effective and green synthesis method of Fe nanoparticles is important. However, Fe nanoparticles were synthesized only in acidic solution by using strong reducing reagent, such as NaBH4, while it cannot be achieved in the basic solution since iron hydroxide, which is easily formed in this condition, cannot be reduced into metals. However, former is not suitable for commercial synthesis method because of its intense reducing process. Thus, the reducing rout of Fe species under basic condition should be developed.Here, until now, we tried to synthesis the various metal/alloy nanoparticles, such as Pd20Te7 nanoparticles and uniform Cu-In (CI) alloy nanoparticles, by utilizing the restrict metallic complexes controlling method in an aqueous solution under room temperature. In this method, reduction potential of the metal species can be controlled under the room temperature, consequently metal/alloy nanomaterials with uniform and well crystallized structure can be successfully synthesized.So, in this study, we proposed the synthesis method of iron particles under basic conditions, and morphological conditions control of iron particles by utilizing the “gel-sol” method using gel networks, which is a safe and simple approach to suppress the chain crystallization.Calculation results indicated that Fe complex can be restricted into one species as the function of pH, complexing reagent species and/also concentration. For example, Fe complex can be restricted into single complex in the basic condition (for example 8<pH<11).By using this homogeneous condition, metal Fe nanoparticles were successfully synthesized. The addition of gel networks into the synthesis system can effectively suppress the chain crystallization of iron particles. Furthermore, in order to obtain iron particles with organic-free surface, we proposed a novel method to decompose and remove the gel networks.Other results will present in our presentation. This work was supported by JSPS KAKENHI Grant Number 21H03628 and DOWA holdings.[1] H. Takahashi et.,al, Applied Catalysis A: General 392 (2011) 80–85

Fabrication of Biogenic Titanium Nanoparticles and Investigating Their Biological Properties for Dental Applications.

Oral inflammation, often triggered by infections, injuries, or immune responses, can compromise treatment outcomes, delay healing, and contribute to patient discomfort. The development of green nanoparticle synthesis methods is receiving attention due to their potential advantages over existing approaches. These procedures use commonly available, affordable, and environmentally friendly natural plant extracts. Due to their numerous uses in various industries, titanium oxide nanoparticles (TiO2NPs) have attracted the most attention among the nanoparticles. In this study, we present the green synthesis of Myristica fragrans (mace) extract as a reductant and stabilizer for the production of curcumin-functionalized TiO2NPs (CTN). We additionally evaluated the effectiveness of these nanoparticles as anti-inflammatory agents. In this study, we aim to develop biogenic TiO2NPs using Myristica fragrans as a natural capping agent and functionalized with curcumin for effectively managing oral inflammation in dental applications. The nanoparticles were synthesized using the green synthesis method and characterized using various characterization techniques. Biocompatibility was evaluated using hemolytic assays, and the bioactivity of the nanoparticles was assessed using anti-inflammatory assays. Curcumin-coated M-TiO2NPs (MCTN) were successfully synthesized and characterized by various techniques, confirming their morphology, crystallinity, functionalization, elemental composition, size, and stability. In vitro bioactivity studies revealed that MCTN exhibited significant anti-inflammatory activity, as evidenced by the inhibition of protein denaturation with minimal hemolytic potential. These findings highlight the potential of MCTN as a promising candidate for anti-inflammatory applications. Our results suggest that MCTN exhibits promising anti-inflammatory and anti-hemolytic properties. However, further in-depth in vivo analysis is required to fully understand their efficacy and toxicity.

Ultrasound-assisted synthesis of PDA nanoparticles with controllable size regulation

The particle size is of importance for polydopamine (PDA) nanoparticles in different applications. Therefore, the synthesis approaches of PDA nanoparticles with fine-controlled size are highly desirable. Here, a novel ultrasound-assisted strategy for size control preparation of PDA nanoparticles was reported. The incorporation of ultrasound facilitated the reaction kinetics of DA, which increased the nucleation rate of PDA nanoparticles, therefore yielding smaller PDA nanoparticles. The diameter of PDA nanoparticles can be regulated effectively from 162.8 to 666.7 nm through adjusting dopamine (DA) concentration from 1 to 4 mg/mL in ultrasound-assisted route. This work has enriched the controllable synthesis methods of PDA nanoparticles, which opens more room for size dependent applications of PDA nanoparticles.

One-pot biogenic synthesis of gold nanoparticles@saponins niosomes: Sustainable nanomedicine for antibacterial, anti-inflammatory and anticancer therapeutics

Nanomedicine offers the promise of transforming modern medicine by facilitating precise and targeted therapeutic outcomes while reducing the adverse effects associated with conventional therapies. Nevertheless, the production process of nanomedicine often involves the use of hazardous and costly chemicals, leading to the creation of toxic by-products. This research paper presents a newly-developed, eco-friendly, one pot method of gold nanoparticles (AuNPs)@saponins niosomes synthesis by utilizing Sapindus mukorossi pericarps extract as both a reducing agent and stabilizer. The properties of the resulting AuNPs@saponins niosomes were analyzed in terms of composition, chemical structure, and morphology. Antibacterial activity of AuNPs@saponins niosomes was observed at concentrations of 50–100 µg/mL, and showed significant antibacterial effect on Staphylococcus aureus. The synthesized AuNPs@saponins niosomes also demonstrated potential as an anti-inflammatory agent, exhibiting an IC50 of 30.08 µg/mL, and displayed a dose-dependent anticancer effect against various human cancer cell lines up to 100 µg/mL. Among the tested cell lines, AuNPs@saponins niosomes exhibited the most effective cytotoxicity on MCF-7 cancer cells, displaying an IC50 of 54.13 µg/mL. Our results suggest that, this research has the potential to uncover the opportunities in the development of environmentally sustainable one-pot synthesis methods of metallic nanoparticles loaded niosomes, with applications in various fields for antibacterial, anti-inflammatory and anticancer therapeutics, offering promising avenues for advancement.

Insights into the bacterial synthesis of metal nanoparticles

Metal nanoparticles have attracted considerable attention due to their astounding potential for a wide range of commercial applications. From targeted drug delivery and antimicrobial agents to electronics, metal nanoparticles seem to have immeasurable prospects in all areas of science. However, modern industrial production frequently involves complex procedures, large amounts of energy, utilizes strong chemical solvents, or produces hazardous waste. Biological synthesis has been proposed as an alternative for simpler, inexpensive, and more eco-friendly metal nanoparticle production. Microorganisms possess multiple mechanisms to transport, regulate and bind metal ions that may result in the biosynthesis of nanoparticles. They can synthesize even complex bimetallic nanoparticles, which are difficult to produce with normal chemical and physical processes. A better understanding of bacteria-metal interactions might thus pave the way for a wide array of industrial applications. This review will summarize the current methods for metal nanoparticle synthesis, with a focus on the microbial (bio) synthesis of nanoparticles. We will describe the general mechanisms of bacteria-metal ion interactions, including cellular uptake and the subsequent reduction into nanoparticles. Protocols for the production of metal-based nanoparticles of relevant elements with different bacterial strains are compiled and the current challenges in bacterial synthesis of metal nanoparticles in the industry are discussed.

Green synthesis of silver nanoparticles via Taxus wallichiana Zucc. plant-derived Taxol: Novel utilization as anticancer, antioxidation, anti-inflammation, and antiurolithic potential

Abstract Throughout history, the utilization of plant products as medicinal remedies has been widespread, with numerous modern drugs finding their origins in the plant kingdom. Taxol, derived from Taxus species, stands out as an exceptional and highly potent anticancer medication. In this study, we present a rapid one-pot synthesis method for silver nanoparticles (AgNPs) using the leaves of Taxus wallichiana Zucca in the presence of sunlight. The synthesized AgNPs were comprehensively characterized using X-ray diffraction, transmission electron microscopy, and HPLC Q-TOF. The AgNPs were further investigated for their antioxidative, anticancer, anti-inflammatory, and antiurolithi properties. The anticancer activity was assessed through a sulforhodamine B assay conducted on the MDA-MB-231 human breast carcinoma cell line and SiHa human cervical cancer cell line. The findings of this study reveal the impressive antioxidative, anticancer, anti-inflammatory, and antiurolithi characteristics exhibited by AgNPs synthesized from leaf extracts. This research highlights an environmentally friendly and cost-effective approach to producing AgNPs by utilizing plant extracts as reducing agents, underscoring the immense potential of natural resources in advancing nanotechnology and its applications.

A Green Silver Nanoparticles using Pistachio Wastes for Dyes Degradation Exposing Visible Light and Reduction of 4‐Nitrophenol

AbstractA simple, green method is described for the synthesis of Silver (Ag) nanoparticles (NPs) from the peel extract of Pistachio. The synthesized particles were characterized using UV‐Vis, FT‐IR spectroscopy, XRD, EDAX, FESEM, and TEM images. The effect of pH of the reaction, time, temperature, and amount of Pistachio peel on the synthesis of AgNPs was evaluated. Unlike other biological methods for NPs synthesis, the uniqueness of our method lies in its appropriate synthesis rates and the ability to tune the nanoparticle size via the used extract in the experiment. AgNPs were used as a heterogeneous photocatalyst for the degradation of eosin Y under visible light. Also, the reduction of 4‐nitrophenol to 4‐aminophenol by AgNPs was investigated and after 15 min this reaction was completed. These results demonstrate a very efficient, cost‐effective, and benign method in the field of synthesis of nanoparticles and catalysis technology development.

The green synthesis of nanoparticles using Costus pictus leaf extract blended with paint and its potential applications

Nanoparticles are important due to their unique properties that may lead to new and exciting applications. Green synthesis has many advantages amongst physical and chemical methods of nanoparticle synthesis since the method is simple, inexpensive, and environmentally friendly. This research work describes the synthesis of carbon nanoparticles using leaf extract of Costus pictus and the characterization studies were carried out by Fourier Transform Infra-Red and Ultra Violet-Visible spectrophotometry. Incorporation of the nanoparticles of Costus pictus leaf extract into the paint formulation increases the corrosion protection properties. The inhibitive effects were investigated using weight loss, polarization, and impedance studies. The surface analysis techniques of Scanning Electron Microscope and Atomic Force Microscopy were used to investigate the morphology study. Findings show that synthetic nanoparticles mixed with paint function as more effective and thermally stable agents, serving as an alternative environmentally acceptable anticorrosion addition for cleaning and pickling processes in industries.

Revisiting the Green Synthesis of Nanoparticles: Uncovering Influences of Plant Extracts as Reducing Agents for Enhanced Synthesis Efficiency and Its Biomedical Applications.

Conventional nanoparticle synthesis methods involve harsh conditions, high costs, and environmental pollution. In this context, researchers are actively searching for sustainable, eco-friendly alternatives to conventional chemical synthesis methods. This has led to the development of green synthesis procedures among which the exploration of the plant-mediated synthesis of nanoparticles experienced a great development. Especially, because plant extracts can work as reducing and stabilizing agents. This opens up new possibilities for cost-effective, environmentally-friendly nanoparticle synthesis with enhanced size uniformity and stability. Moreover, bio-inspired nanoparticles derived from plants exhibit intriguing pharmacological properties, making them highly promising for use in medical applications due to their biocompatibility and nano-dimension. This study investigates the role of specific phytochemicals, such as phenolic compounds, terpenoids, and proteins, in plant-mediated nanoparticle synthesis together with their influence on particle size, stability, and properties. Additionally, we highlight the potential applications of these bio-derived nanoparticles, particularly with regard to drug delivery, disease management, agriculture, bioremediation, and application in other industries. Extensive research on scientific databases identified green synthesis methods, specifically plant-mediated synthesis, with a focus on understanding the contributions of phytochemicals like phenolic compounds, terpenoids, and proteins. The database search covered the field's development over the past 15 years. Insights gained from this exploration highlight plant-mediated green synthesis for cost-effective nanoparticle production with significant pharmacological properties. Utilizing renewable biological resources and controlling nanoparticle characteristics through biomolecule interactions offer promising avenues for future research and applications. This review delves into the scientific intricacies of plant-mediated synthesis of nanoparticles, highlighting the advantages of this approach over the traditional chemical synthesis methods. The study showcases the immense potential of green synthesis for medical and other applications, aiming to inspire further research in this exciting area and promote a more sustainable future.

Application of nanoparticles in modified polybenzimidazole-based high temperature proton exchange membranes

Polymeric proton exchange membranes (PEMs) are vital components of fuel cells, as they enable the transport of protons while preventing the crossover of fuel and oxidant gases. However, conventional PEMs have limitations such as low use temperature, low proton conductivity, and poor mechanical and thermal stability. Various types of nanoparticles have been investigated to modify PEMs to overcome these limitations, as they can increase proton conductivity, mechanical strength, thermal stability, and chemical resistance. Metal oxides such as SiO2 and TiO2 have been shown to improve the proton conductivity and mechanical properties of PEMs. Carbon-based materials such as graphene oxide have been found to enhance both the proton conductivity and thermal stability of PEMs. The use of nanoparticles in modified polymeric PEMs for fuel cells shows excellent potential for improving the performance and durability of fuel cells. Future research should focus on developing cost-effective and scalable methods for nanoparticle synthesis and incorporation into PEMs. Polybenzimidazole (PBI) is the most widely studied high-temperature polymer for preparing composite PEMs. This review provides the recent development of PBI composite PEMs modified with different types of nanoparticles.

Thermal and chemical structure of ethanol and 2-ethylhexanoic acid/ethanol SpraySyn flames

Flame spray pyrolysis is intensively developing as a promising method of nanoparticle synthesis. The experimentally measured chemical and thermal structure of the spray flame is critically needed as a validation target for simulations of the synthesis of nanoparticles in these spray flames. This paper presents an experimental study of the chemical and thermal structure of the flame produced by the SpraySyn burner. The spatial distribution of the main species is obtained by microprobe sampling in combination with orthogonal time-of-flight mass spectrometry. The temperature distribution is obtained by a micro-thermocouple technique. The presented species and temperature data are discussed in the context of their experimental uncertainties and the plausibility of the flame structure.

Sustainable Nanotechnology- Green Synthesis Methods for Silver Nanoparticles

Silver has a long history of being an excellent substance in its field of medicine Because of their special qualities, they have been used for a wide range of products, including antibacterial agents, household, industrial, and healthcare-related items, coatings for medical devices, drug delivery, and eventually to increase the tumor-killing effects of anticancer drugs. By the help of nanotechnology pure silver nanoparticles is obtained. The bioreduction of metal ions into their elemental form in the size range of 1–100 nanometers (nm) is known as "green synthesis," and it requires the use of plants or plant parts. As a result, the biological activities are increased and the toxicity level is decreased when AgNPs are synthesised using a green method. Scientists and researchers from various fields are becoming more and more interested in synthesising nanoparticles, particularly silver nanoparticles. However, they are unsure of the proper procedures to follow and the safety precautions to take when synthesising AgNPs, which is why the main goal of this paper is to provide a detailed methodology for the environmentally friendly synthesis of silver nanoparticles.

Algal Nanoparticles and Their Antibacterial Activity: Current Research Status and Future Prospectives

Green nanotechnology is a promising technology that has a wide range of applications in pharmaceuticals today because they offer a higher surface-area-to-volume ratio. Algal-based nanoparticles (NPs) are the subject of intense research interest today for their potential to treat and prevent infections caused by infectious microorganisms that are antibiotic resistant. Algae contain a variety of therapeutically potential bioactive ingredients, including chlorophyll, phycobilin, phenolics, flavonoids, glucosides, tannins, and saponins. As a result, NPs made from algae could be used as therapeutic antimicrobials. Due to their higher surface-area-to-volume ratios compared to their macroscopic components, metallic nanoparticles are more reactive and have toxic effects on their therapy. For pharmaceutical and biomedical applications, green synthesis restricts the use of physical and chemical methods of metallic nanoparticle synthesis, and it can be carried out in an environmentally friendly and relatively low-cost manner. The majority of macroalgae and some microalgae have latent antimicrobial activity and are used in the synthesis of metallic nanoparticles. A potential application in the field of nanomedicine and the establishment of a potential pharmacophore against microorganisms may result from the synthesis of algal-based NPs. Only a few studies have been done on the potential antimicrobial, antifungal, and antibacterial activity of algae-based NPs. As a result, the study will concentrate on the environmentally friendly synthesis of various NPs and their therapeutic potential, with a focus on their antibacterial activity. Thus, the aim of this study is to review all the literature available on the synthesis and characterization of the algal nanoparticles and their potential application as an antibacterial agent.

Utilization of Nanomaterials In Prosthetic Dental Treatment

Nanomaterials in prosthetic dental treatments have garnered significant attention in the field of dentistry. These nanomaterials are utilized in various forms, such as nanoceramics, nanocomposites, nanoimplants, and antimicrobial nanoparticles. Particularly, through nanotechnology advancements, these materials offer high mechanical strength, biocompatibility, and aesthetic properties, enhancing the success of dental restorations and prosthetics. Additionally, nanoparticles with antimicrobial properties provide protection against periodontal diseases and tooth decay. The development of nanoparticle synthesis methods and techniques has enabled the use of more effective and safe materials in prosthetic dental treatments. The impact of nanotechnology in dentistry allows for the development of more successful and sustainable treatment options for patients.

Structural and Functional Analyses of the Acidic and Basic Amino Acid Repeat Sequence (DDRK) in Pif 80 from Pinctada fucata on the Aragonite Crystal Surface Using NMR

Shellfish and crustaceans use calcium carbonate (CaCO3) as a protective shell and exoskeleton. CaCO3 is a major biomineral component in invertebrates. The Pinctada fucata shell contains two crystal polymorphs, with outer prismatic and inner nacreous layers. Diverse shell organic materials play significant roles in microstructure formation with organic–inorganic interactions. Pif 80 has a repeated acidic and basic amino acid low-complexity region that specifically binds to aragonite crystals. In this study, we aimed to elucidate the microstructure formation mechanism by analyzing the interaction between the low-complexity region and CaCO3. We used solution NMR to evaluate the structure and activity of the Pif 80 acidic region. We designed synthesis methods for aragonite nanoparticles that can be dispersed and rotated in solution. The results revealed that the “GDDRKDDRKG” peptide has the appropriate structure for aragonite surface binding, wherein the four carboxy groups of the Asp side chain are in the same plane, supported by Arg and Lys residues. The saturation transfer difference also supported this structure. These results suggest that the Pif 80 acidic region binds the aragonite crystal surface via Asp side chains with basic residues, providing new insights into the function of acidic and basic amino acids in biomineral formation.

Synthesis and Evaluation of Platinum Nanoparticles Using F. Carica Fruit Extract and Their Antimicrobial Activities

Abstract In this manuscript, a simple new method for the green synthesis of platinum nanoparticles (Pt NPs) utilizing F. carica Fig extract as reducing agent for antimicrobial activities was reported. Simultaneously, the microstructural and morphological features of the synthesized Pt NPs were thoroughly investigated. In particular, the attained Pt NPs exhibited spherical shape with diameter range of 5-30 nm and root mean square of 9.48 nm using Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM), respectively. Additionally, the final product (Pt NPs) was screened as antifungal and antibacterial agent against Candida and Aspergillus species as well as Gram-positive Staphyllococcus aureus and Gram-negative Acinetobacter species, respectively. Accordingly, the synthesized NPs demonstrated inhibition zones of 36 and 28 mm against fungal and bacterial species, respectively. The presented Pt NPs play an active role in both antifungal and antibacterial activities which indicates the presence of a well-regulated nano-materials system for biomedical application.