Simple SummaryFish are an important source of proteins of a high biological value, of some vitamins and minerals, and of polyunsaturated omega-3 fatty acids. However, fish products can also allow harmful substances, like heavy metals, to enter the diet. Such substances are recognized as being the most serious contaminants for aquatic ecosystems at the present time. Their non-biodegradability allows them to accumulate in fish tissues, and then pass into human diets. Therefore, our study aimed to determine the concentrations of heavy metals (As, Cd, Cu, Hg, Ni, Pb, and Zn), and to evaluate the bioaccumulation patterns in the different types of musculature in two species of fish of commercial interest, Chelon auratus and Chelon saliens, from the south coast of the Caspian Sea. The results obtained emphasize the need to continue to monitor and evaluate the degree of pollution in the sampled area, both in fish and other species and also in the environment, as well as recommending prevention measures orientated towards limiting and/or reducing the excessive exposure of the human population to heavy metal contamination.Although fish is a food that supplies nutrients of a high biological value, they can also be a source of some harmful substances, such as heavy metals. In the same context, some human activities in the Caspian Sea have contaminated this ecosystem during the past few years. For those reasons, our objective consisted of determining the concentrations of heavy metals and evaluating their bioaccumulation patterns in the different types of musculature in two species of mullets of commercial interest, Chelon auratus and Chelon saliens, from the southern coast of this sea. For this purpose, 20 C. auratus and 29 C. saliens were caught off this coastline and the metal concentrations in 3 different muscle locations were analyzed: the ventral, dorsal and caudal muscles of each fish. The caudal muscle had higher concentrations of Cd, Cu, Pb, and Zn, whereas As, Hg and Ni accumulation seemed to be independent of the musculature type. Overall, the Cd, Hg, and Pb concentrations exceeded the maximum levels permitted in fish by the European Union. In addition, the relationships between pairs of metals were positive and elevated in all the cases, which could be a sign of heavy metal pollution in the region sampled. Therefore, it will be necessary to continue monitoring and evaluating the degree of pollution in the Caspian Sea.