Bone Tissue Metabolism Research Articles

Application of ultrasonic treatment and synthesized collagen hydrolyzate in the surgical treatment of experimental osteomyelitis

Chronic osteomyelitis is one of their complex health problems, since it accounts for up to 6% of cases in the structure of the pathology of the musculoskeletal system, relapses of the disease occur in 30%, and disability is 90% of cases.The aim of the study was to improve the results of treatment of experimental chronic osteomyelitis by applying ultrasound sanation and synthesized collagen hydrolyzate.Materials and methods. The study was carried out on 210 white rats, which were divided into 5 groups: 2 control rats and 3 experimental ones. In the 1st control group, no treatment was performed. In the 2nd control and experimental groups, the hearth was surgically sanitized. In the 1 st experimental group, the focus was performed by ultrasonic treatment. In the 2nd experimental group, the bone cavity was filled with a synthesized hydrolyzate of collagen. In the third experimental group, the combined use of ultrasound sanitation of the focus and filling it with synthesized collagen hydrolyzate was carried out. To assess the dynamics of treatment, the analysis of the general condition of animals, hematological and morphometric studies was performed.Results and their discussions.The dynamics of the general state of animals, the antioxidant defense system, the mineral metabolism, and the evaluation of the histoarchitectonics of bone tissue have been studied. It was noted that against the backdrop of the inflammatory process in chronic osteomyelitis, all the studied indicators indicate a reduced regenerative capacity. Using the developed treatment methods based on ultrasound sanitation, synthesized collagen hydrolyzate, as well as their combination, accelerated relief of inflammatory phenomena, normalization of AOS, mineral metabolism, and regenerative abilities of bone tissue were observed.Conclusions. The developed complex method of treatment based on combined application of ultrasound sanation and synthesized collagen hydrolyzate allowed to shorten the healing period of the wound defect, normalize the general condition of animals, free radical oxidation and mineral metabolism parameters, and accelerate the regenerative capabilities of bone tissue.

ВЛИЯНИЕ КРЕМНИЙСОДЕРЖАЩЕЙ ВОДЫ «АРДВИ» НА РЕГЕНЕРАЦИЮ МЯГКИХ ТКАНЕЙ И ДОЛГОСРОЧНУЮ ОСТАВЛЕННУЮ МЫШЕЧНУЮ БОЛЕЗНЕННОСТЬ

Aim. The purpose of this study is to investigate the influence of silicon-containing water “ARDVI” on wound healing and long-term delayed onset muscle soreness by means of experiment and on-site observations. Material and Methods. As the source of silicon, we used still natural mineral table water “ARDVI”, produced according to technical specifications 9185-004-37881001-12 and extracted from well No. 988, located in 300 meters to Southeast from the village of Kasargi in the Chelyabinsk region. In order to study the regenerative capacity of the water we formed three groups of white male rats from Wistar line weighing 130–160 g, each group consisted of 10 animals. Results. We gave the characteristic of silicon as an essential nutrient involved in the metabolism of muscle, connective and bone tissues. We studied the chemical composition and physico-chemical parameters of silicon-containing water “ARDVI”. The functional properties of this water were studied in experiments on male rats from Wistar line. Animals for 14 days received through the tube 25 ml of mineral natural water “ARDVI” in addition to their drinking regime. To assess the regenerative properties of this water we inflicted cut wound on the shaved skin of rats from the experimental group and calculated its area during the whole experiment. Based on the study of the dynamics of wound surface area in animals from control and experimental groups, we made a conclusion on the influence of “ARDVI” water on the regeneration of soft tissues. We studied water effect on restoration of the physical activity of athletes-football players and their enzymatic status. We registered the reduction of the syndrome of long-term delayed onset muscle soreness, which indicates the positive impact on the syndrome of long-term delayed onset muscle soreness, confirmed by the indexes of fatigue and muscle tissue damage, as well as by De Ritis ratio. Conclusions. The use of natural mineral water “ARDVI” is important for the correction of the metabolic processes of muscle, connective and bone tissues, as well as for the prevention and complex treatment of possible pathologies connected with physical culture and sports. The water studied may be used as the basis for the development of other specialized sports nutrition products with essential nutrients and natural plant material with unidirectional functional properties.

Effects of long-term oral administration of methimazole on femur and tibia properties in male Wistar rats

Physiological concentrations of thyroid hormones are crucial for skeletal growth and development, physiological bone turnover and bone homeostasis maintenance. Methimazole (1-methyl-2-mercaptoimidazole) is an antithyroid drug used for the treatment of the hyperthyroidism in humans and animals. The aim of the study was to determine effects of long-term oral methimazole treatment in male Wistar rats on biochemical bone metabolism markers, as well as morphological, geometric, densitometric and mechanical properties of femur and tibia. Experimental rats were subjected to 90-day-long oral treatment with 0.05% water solution of methimazole and were kept under identical environmental conditions and received the same diet ad libitum as the control group. Serum concentration of osteocalcin (OC) and C-terminal telopeptides of type I collagen (CTX-I) was determined. Femur and tibia were evaluated using quantitative computed tomography (QCT), peripheral QCT (pQCT) and three-point bending test. Final body weight of the experimental group was significantly decreased by 30% (P=0.01). Methimazole treatment significantly decreased serum OC concentration by 21% (P=0.02) and increased CTX-I concentration by 17% (P=0.06). Methimazole decreased morphological, geometric and densitometric parameters of femur and tibia in rats. Mechanical evaluation of bones has shown significantly decreased maximum elastic strength and ultimate strength of femur in rats treated with methimazole by 36% and 40% when compared to the control group (P<0.05). In conclusion, this study has shown that long-term treatment with methimazole inhibits bone formation and accelerates bone resorption processes. The observed negative effects of methimazole treatment on body weight gain and skeletal properties may be considered as additional possible side effects in living organisms to those reported in the previous studies. It may be suggested that long-term antithyroid treatment should be combined with prevention of the negative effects of methimazole on bone tissue and whole body metabolism.

Ocena zaburzeń w układzie kostnym u dzieci z nerwiakowłókniakowatością typu 1

IntroductionBone abnormalities occur often in patients with neurofibromatosis type 1 (NF1). This suggests that the metabolism of bone tissue may be affected in these patients. ObjectiveEvaluation of markers of bone mineral density and bone metabolism with respect to bone abnormalities in patients with NF1. MethodsA total number of 57 NF1 patients and 145 controls were studied. Activity or concentrations of following markers of bone mineralization and bone metabolism were performed on all patients: bone-specific alkaline phosphatase (BAP), osteoprotegerin (OPG) and collagen type I crosslinked C-telopeptide (CTX). ResultsBone abnormalities were observed in 75.44% NF1 patients. NF1 patients had a significant decrease in BMD z-score compared with controls (–0.679±1.157 vs 0.000±1.000g/cm2, p<0.0001). BAP activity was significantly higher in NF1 patients compared with controls (97.7±46.2 vs 74.1±29.7 U/l, p<0.0001). CTX concentration for NF1 group was significantly higher compared with controls (1.89±0.78 vs 1.45±0.72 ng/ml, p=0.002). No significant differences in OPG (3.94±1.07 vs 3.72±1.45pmol/l) and sRANKL (0.147±0.175 vs 0.148±0.134pmol/l) values were found between analyzed groups. ConclusionsSceletal defects often occurs in NF1 patients, with scoliosis being the most common pathology. NF1 can predispose to premature loss of bone mass. Analyzed markers indicate that bone resorption predominates over ossification in NF1 patients.

System epidermal nevus with hyperkeratosis and violations of bone tissue metabolism -- therapy of drug of pamidrinic acid and surgical orthopedic treatment. Case from practice

System epidermal nevus with hyperkeratosis and violations of bone tissue metabolism -- therapy of drug of pamidrinic acid and surgical orthopedic treatment. Case from practice

Own research experience of bone tissue metabolism in patients with the Ehlers-Danlos syndrome

Own research experience of bone tissue metabolism in patients with the Ehlers-Danlos syndrome

Polymorphism of some genes of bone tissue metabolism (VDR Bsm1 c.IVS7G&gt;A, LCT 13910 T&gt;C, COL1A 12046 G-&gt;T) among the representatives of Russian and Buryat nationalities

Aim: to study the frequency of genotypes of the polymorphous markers of bone remodeling (vita-min D receptor gene Bsm1 c.IVS7G&gt; A, the lactase gene LCT 13910 T&gt; C and collagen gene COL1A 12046 G-&gt; T) in healthy people and patients with osteopo-rosis (OP) among the indigenous population of Trans-baikalia of Russian and Buryat nationalities.&#x0D; Methods: 97 women with OP were examined: 49 Russian and 48 Buryat women aged from 50 to 80 years. 123 healthy women of the same age group were included in the control group. DNA sam-ples for molecular genetic analysis were taken from peripheral venous blood.&#x0D; Results: The recessive allele A of the VDR – Bsm1c.IVS7G&gt; A polymorphism was accumulated in women of Buryat nationality, but the statistical significance was not observed (OR = 1.04, CI [0 68, 1.6]). C al-lele of LCT -13910 T&gt; C polymorphism was associated with the development of OP among the representa-tives of Buryat nationality.&#x0D; Conclusion: The gene allele VDR Bsm1 c.IVS7G&gt; Aand the LCT -13910 T&gt;C leads to a higher risk of OP in women of Buryat nationality. The genotypes G/Tand T/T of COL1A12046 G-&gt; T are associated with the development of OP in people of both nationalities.

VITAMIN D REGULATION OF BONE METABOLISM

The main vitamin D (1,25(OH)2D) function lies in maintaining of calcium and phosphates normal concentrations in a blood plasma that is necessary for adequate bone tissue mineralization and skillet formation. Now there’s no doubt that vitamin D immediately participates in the bone tissue metabolism, directly influencing through VDR receptors located on cells: chondrocytes, osteoblasts, osteocytes and osteoclasts. At that, mechanisms of calcium and phosphorus intestinal absorption stimulation and immediate vitamin D influence on bone cells are significant and complementary in the full-fledged bone tissue metabolism regulation process.

Would Interstitial Fluid Flow be Responsible for Skeletal Maintenance in Tail-Suspended Rats?

Despite the fast development of manned space flight, the mechanism and countermeasures of weightlessness osteoporosis in astronauts are still within research. It is accepted that unloading has been considered as primary factor, but the precise mechanism is still unclear. Since bone’s interstitial fluid flow (IFF) is believed to be significant to nutrient supply and waste metabolism of bone tissue, it may influence bone quality as well. We investigated IFF’s variation in different parts of body (included parietal bone, ulna, lumbar, tibia and tailbone) of rats using a tail-suspended (TS) system. Ten female Sprague-Dawley (SD) rats were divided into two groups: control (CON) and tail-suspension (TS) group. And after 21 days’ experiment, the rats were injected reactive red to observe lacuna’s condition under a confocal laser scanning microscope. The variations of IFF were analyzed by the number and area of lacuna. Volumetric bone mineral density (vBMD) and microarchitecture of bones were evaluated by micro-CT. The correlation coefficients between lacuna’s number/area and vBMD were also analyzed. According to our experimental results, a 21 days’ tail-suspension could cause a decrease of IFF in lumbar, tibia and tailbone and an increase of IFF in ulna. But in parietal bone, it showed no significant change. The vBMD and microarchitecture parameters also decreased in lumbar and tibia and increased in ulna. But in parietal bone and tailbone, it showed no significant change. And correlation analysis showed significant correlation between vBMD and lacuna’s number in lumbar, tibia and ulna. Therefore, IFF decrease may be partly contribute to bone loss in tail-suspended rats, and it should be further investigated.

Prevention of osteoporosis with the use of the product of specialized medical nutrition

Aim. To study the efficacy of shellfish dry extract with «Calcium-reparative» filler as a product of specialized medical nutrition to optimize bone metabolism.Methods. Total of 44 female patients aged from 49 to 71 years, mean age of 54.5±3.8 years, with a combination of several osteoporosis risk factors were involved. 72.7% of all patients complained of pain of varying severity in major joints, in the thoracic and lumbar spine. The patients were randomly divided into two groups - study group (29 patients) and control group (15 patients). Within 30 days, the participants of the study group received «Calcium-reparative» - a product created on the basis of natural biologically active compounds isolated from Far Eastern marine shellfish and purified Altai Shilajit. The efficacy of the product was assessed by means of standard questionnaires for the assessment of quality of life, 10-point visual analogue scale, biochemical laboratory tests. Measurement of serum osteocalcin was performed by ELISA, and immunochemical method - N-terminal propeptide of procollagen type I (the biomarker of bone tissue metabolism).Results. Adverse and allergic reactions to the product were not registered. Among those receiving the product 37.9% noted improvement of well-being and reduction of pain intensity from 6.9±0.45 to 4.28±0.74 points. In those receiving «Calcium-reparative» the level of synthesis of osteocalcin in the study group did not statistically significantly change and appeared to be 18.6±3.8 ng/ml. The increase in the concentration of procollagen type I from 20.9±3.0 ng/ml prior to administration of the therapeutic product to 37.6±5.8 ng/ml (t=0.006, p ≤0.001) after its administration was observed.Conclusion. Product of medical nutrition «Calcium-reparative» has the ability to stimulate the cells of bone tissue to synthesize bone matrix protein collagen type I.

Research of the features of cardiovascular remodeling and bone tissue metabolism markers changes in women with coronary artery disease, associated with postmenopausal osteoporosis

The coronary artery disease is the one of most urgent problems of modern cardiology as well as the problem of osteoporosis. The aim of research was to study the features of cardiovascular remodeling and bone tissue metabolism markers changes in women with coronary artery disease, associated with postmenopause osteoporosis.Methods of research. There were examined 78 women in postmenopause period with coronary artery disease (CAD): 1 group (comparison) – 19 women with CAD and normal indices of the bone tissue mineral density (BTMD); 2 group – 27 women with CAD and osteopenia; 3 group – 32 women with CAD and osteoporosis; the control group – 12 conventionally healthy women of corresponding age. With the help of ultrasound densitometry, echocardioscopy and immune-enzyme analysis the state of bone tissue mineral density, structural-functional state of heart and level of bone remodeling biomarkers were studied.Results. It was revealed, that CAD clinical course on the background of osteopenic syndrome is characterized with rise of bone remodeling markers level, increase of carotid arteries intima-media thickness, more often development of diastolic dysfunction, calcification of heart valves and big vessels, the correlations between the osteoprotegerin, osteocalcin, homocysteine levels and cardiovascular remodeling parameters were established.Conclusions. For women in postmenopause period with coronary artery disease, comorbid with osteoporosis is typical the rise of bone metabolism markers (osteoprotegerin, osteocalcin) levels and endothelial dysfunction (homocysteine) that not only reflect the state of bone tissue but also take part in cardiovascular remodeling

CLIMATE AND NEWBORN INFANTS BIRTH GROWTH AND WEIGHT IN RUSSIA

Aim. The aim of the study was to monitor the correlation between the infants birth growth weight and the climatic characteristics using regional statistical indices. Materials and methods. Mean birth growth and birth weight values of 790 276 newborn infants from 42 subjects of the Russian Federation with the share of the Russian population not less than 85 % (2007) were taken from the database of Social Insurance Fund. Mean temperature of July and January, mean wind speed, average annual horizontal surface insolation (kw·day/m2) were used as the parameters characterizing climatic conditions of the region. Climate severity was estimated by Bodman index values and annual temperature difference. Results. Newborn infant’s birth weight is decreasing with climate severity growing: it correlates with the average temperature of January ( r = + 0,56), Bodman index values ( r = -0,49) and interseasonal (July-January) temperature difference ( r = -0,54). Newborn infant’s birth growth is directly connected with insolation level ( r = 0,31) that agrees with the notion of the role of UV-radiation in metabolism of fetal bone tissue. Conclusions. 1. Newborn infant’s birth weight is decreasing with fall in a mean temperature of January, increase in climate severity and growth of season temperature difference. 2. Newborn infant’s birth growth is increasing with rise in an average annual insolation level.

Pathology and mineral composition of bone in the early stages of experimental osteomyelitis

Актуальность Изучение патогенетических закономерностей и характера структурных изменений в костной ткани на ранних стадиях острого остеомиелита является актуальным с точки зрения расширения представлений о механизмах развития осложнений у больных и проведения комплекса реабилитационных мероприятий.Цель работы Комплексная оценка патоморфологических изменений и особенностей минерального состава костной ткани на ранних стадиях развития острого остеомиелита в эксперименте.Материалы и методы Эксперименты проведены на 35 крольчатах обоего пола, в соответствии с биоэтическими требованиями. Острый гематогенный остеомиелит моделировали путем введения культуры стафилококка в костномозговой канал большеберцовой кости. Всех животных выводили из опыта под кратковременным эфирным наркозом путем воздушной эмболии через 30 мин. 6, 12, 24 и 48 часов после введения культуры стафилококка. На данных сроках эксперимента методами световой, электронной (сканирующей и трансмиссионной) микроскопии и рентгеновского электронно-зондового анализа изучались особенности патоморфологии и минерального состава костной ткани.Результаты и их обсуждение Во время первых 30 минут эксперимента микро- и ультрамикроскопические изменения в костной ткани были минимальными: сохранялись костные стержни с небольшими группами эритроцитов, а минеральный состав кости характеризовался снижением объемной доли натрия. На 6 часе эксперимента также не наблюдалось значительных структурных перестроек в костной ткани, однако происходило дальнейшее снижение концентрации натрия и магния, и увеличение объемной доли серы. В то же время, на данном сроке эксперимента отмечено повышение объемных долей кальция и фосфора. Морфологическая картина в последующие 12 часов эксперимента характеризовалась началом деструктивных процессов в костном мозге, с чем, вероятно, связаны некоторые перестройки минерального обмена костной ткани. Наблюдали резкое повышение концентрации натрия, при этом сохранялись высокие значения объемных долей внутрикостного кальция, фосфора и серы, что отражало повышенную минерализацию. К концу 1 суток эксперимента развивались процессы деструкции костной ткани, которые сопровождались деминерализацией. На 2 сутки отмечалась активация процессов остеогенеза, при этом деминерализация сохранялась.Заключение Таким образом, в течение 1-х суток от начала эксперимента структурные перестройки костной ткани отсутствуют, а изменения ее минерального состава связаны с процессами адаптации в ответ на активное воспаление в костномозговом канале. На 2-е сутки опыта развивающаяся деструкция костной ткани сопровождается явлениями остеопороза наряду с активацией остеосинтеза. Период в течение 1-х суток от попадания возбудителя можно рассматривать как наиболее благоприятный с точки зрения профилактики последующих осложнений.

Negative Effects of Total Gastrectomy on Bone Tissue Metabolism and Volumetric Bone Mineral Density (vBMD) of Lumbar Spine in 1-Year Study in Men.

Gastrectomy induces severe osteoporosis in humans but its quantitative scale within trabecular and cortical compartments was not estimated. The aim of the study was to determine changes of volumetric bone mineral density (vBMD) in lumbar vertebrae (L1–L4) and biochemical bone metabolism markers in serum of patients 1 year after total gastrectomy. The control group consisted of patients (N = 8) subjected to abdominal surgery due to cardiospasmus. Total gastrectomy was performed in the experimental group (N = 6).Volumetric bone mineral density of trabecular and cortical bone of lumbar spine was measured before (baseline) and 1 year after the gastric surgery using the quantitative computed tomography method. Serum concentrations of insulin, insulin-like growth factor-1, tyroxine, interleukin-6, C-terminal telopeptides of type II collagen and bone formation, and resorption markers were determined at baseline and 1 year later, using ELISA, EIA, and IEMA methods.Total gastrectomy induced significant decrease of vBMD values, up to 16.8% and 10.0%, within the trabecular and cortical bone compartments of lumbar spine (P < 0.05). These negative changes of vBMD were associated with significantly increased serum concentration of bone resorption markers such as deoxypyridinoline, pyridinoline, and C-terminal telopeptides of type I collagen, by 13.5%, 32.2%, and 121.5%, respectively (P < 0.05). Neither vBMD nor biochemical bone turnover markers and hormone concentrations were influenced in the control patients.Dramatic bone loss during the first year in gastrectomized patients has proven dynamic osteoporosis progress indicating an importance of treatment interventions in these patients with emphasis on inhibition of intensive bone resorption processes.

Serum Levels of Osteocalcin and Insulin Resistance in Patients with Impaired Glucose Tolerance or New-Onset Diabetes Mellitus After Liver Transplantation.

Liver transplantation (LT) patients are at high risk of developing new-onset diabetes after transplantation (NODAT). Osteocalcin has been proposed as a mediator between bone tissue and glucose metabolism, but its role in the pathogenesis of diabetes is not defined yet. Our objective was to assess the relationship between serum osteocalcin and glucose metabolism parameters in liver transplantation recipients. A total of 187 liver transplantation patients were cross-sectionally studied, 54 of them developed NODAT. None had been diagnosed of diabetes mellitus prior to transplant. In 133 nondiabetic patients, a 75 g oral glucose tolerance test (OGTT) was performed to assess blood glucose, insulin, and C-peptide levels at baseline and 120 min. Serum total osteocalcin was measured at baseline in all patients.After OGTT, 10.5% of LT patients had NODAT criteria, 51.9% showed impaired glucose tolerance, and 37.6% had normal glucose tolerance. Overall, NODAT prevalence was 36.3%. HOMA-IR was significantly higher in NODAT compared with impaired glucose tolerance and normal glucose tolerance groups (p<0.001). Osteocalcin was inversely correlated to HOMA-IR (r=- 0.16, p=0.05), BMI (r=- 0.27, p=0.000) and waist circumference (r=- 0.21, p=0.005). Patients in the lowest osteocalcin tertile (< 16.5 ng/ml) had significantly higher fasting plasma glucose and HOMA-IR index (p=0.029 and 0.037, respectively) than those in medium or highest tertiles. In multiple linear regression analysis, osteocalcin was negatively associated with fasting plasma glucose (standardized β coefficient-0.16; p=0.041) and 2-h insulin (standardized β coefficient-0.21; p=0.028). Prevalence of NODAT/impaired glucose tolerance is high in liver transplantation patients and is associated with insulin resistance. In these patients total osteocalcin is inversely associated with plasma glucose level and insulin resistance indexes.

Different osteocalcin forms, markers of metabolic syndrome and anthropometric measures in children within the IDEFICS cohort

ObjectiveOsteocalcin (OC), an aboundant non-collagenous bone protein, is inversely associated with parameters of glucose metabolism. Interactions between bone tissue and energy metabolism have not been thoroughly investigated during childhood. This study investigated OC, metabolic parameters and anthropometric characteristics in normal weight and overweight/obese children. MethodsThis study comprised 108 (46 normal weight/62 overweight/obese) Swedish 2–9year old children. Anthropometric data, insulin, glucose, glycosylated haemoglobin (HbA1c), HOMA index, vitamin D, adiponectin, total OC, carboxylated OC (cOC) and undercarboxylated OC (ucOC) were analysed. ResultsNo difference was found for total OC between the normal and overweight/obese groups, with a mean (±SD) value of 82.6 (±2.8) ng/mL and 77.0 (±2.4) ng/mL, (P=0.11), respectively. Overweight children had lower cOC levels, mean 69.1 (±2.2) ng/mL, vs. normal weight children, mean 75.6 (±2.5) ng/mL (P=0.03). The mean ucOC levels of 7.9 (±0.4) ng/mL in overweight children did not differ vs. normal weight children, mean level 7.0 (±0.4) ng/mL, (P=0.067). None of the three OC forms correlated with any of the measured parameters. ConclusionsThe cOC levels were lower in overweight children. There was no correlation between the three OC forms and any of the measured anthropometric or metabolic parameters. OC has been suggested to have a possible metabolic role, but in general the current study in prepubertal children does not support the hypothesis of an association between OC and a positive metabolic profile.

Certain aspects of diagnostic of osteopenia syndrome in traumatology and orthopedics.

The osteopenia syndrome characterizing by progressing decrease of bone tissue mass and lowering of its strength due to damage of its micro-architectonics counts in the most significant medical social diseases. The timely detection of disorders of bone tissue remodeling is a complicated diagnostic task as far as laboratory diagnostic of osteo-deficiency conditions is based on finding indirect indices of bone tissue metabolism. The instrumental methods of evaluation of condition of bone structures provide no possibility to detect their early damages and to manage dynamic observation of implemented anti-resorption therapy. The study was carried out to develop complex approaches to laboratory instrumental diagnostic of osteo-deficiency conditions. The comprehensive laboratory instrumental examination of patients with deforming osteoarthritis of hip joint including evaluation of bone tissue mineral density, indices of calcium phosphorus metabolism, markers of bone resorption and bone formation, activity of inflammation process. The study established activation of bone resorption and inhibition of bone formation in patients with high activity of inflation process.

Сывороточное содержание некоторых медиаторов метаболизма костной ткани у женщин с ревматоидным артритом

Сывороточное содержание некоторых медиаторов метаболизма костной ткани у женщин с ревматоидным артритом

BIOCHEMICAL MARKERS OF BONE TISSUE METABOLISM INFLUENCE ON VULNERABILITY OF ATHEROSCLEROTIC LESIONS

Aim . To assess some of biochemical markers of bone tissue metabolism, in vessel wall in males with coronary atherosclerosis; to evaluate the influence of these markers on vulnerability of atherosclerotic lesion and to figure out the specifics of their spread among unstable plaques of various kinds. Material and methods. Totally, 65 males included at the age 46-79 y. o., admitted to the Clinics of E. N. Meshalkin Novosibirsk Scientific-Research Institute of Circulation Pathology for coronary bypass surgery. During operation, an endarterectomy was performed of coronary arteries. Each specimen of endarterectomy was symmetrically divided to fragments for histological and biochemical studies. By the results of histological analysis, in 193 specimens we found: 19 fragments of intact intima, 102 stable, 72 unstable plaques. Then the types of unstable plaques were established: 1) lipids’; 2) inflammatory-erosive; 3) distrophic-necrotic. In homogenates of the fragments we assessed: osteoprotegerin, calcitonin, osteocalcin, cholesterol. Statistics was done with SPSS (13.0). Results. By the results of the performed histology, in unstable plaques there was higher level of calcification comparing to stable plaques. Biochemical studies showed increased levels of calcitonin and osteocalcin. If to assess the diverse types of unstable plaques, the highest level of these parameters was found in distrophic-necrotic plaques; lowest amount of calcitonin and osteocalcin was found in inflammatory-erosive type. Content of cholesterol in stable and unstable atherosclerotic lesions was about 4 times higher (p<0,01) in comparison with intact intima. Relation of cholesterol with calcitonin and osteocalcin was found, as well. Conclusion. The data shows that increased osteoprotegerin aids the stabilization of lesion, and calcitonin and osteocalcin might be markers of plaque instability, caused by increased calcification.

Сывороточное содержание некоторых медиаторов метаболизма костной ткани у женщин с ревматоидным артритом

Сывороточное содержание некоторых медиаторов метаболизма костной ткани у женщин с ревматоидным артритом