Mesona Palustris Research Articles

Potent antioxidant activity of black grass jelly (Mesona palustris BL) leaf extract and fractions

Black grass jelly (Mesona palustris BL) is an Indonesian traditional food that is rich in antioxidants and believed to have potential for treating various diseases such as diabetes, hypertension, and cancer. The present study aimed to determine the antioxidant activity of black grass jelly leaf extract as well as fractions using ABTS, DPPH and FRAP methods. Following ethanol extraction and subsequent fractionations, total flavonoid level was determined, followed by antioxidant activity tests using the ABTS, DPPH and FRAP with vitamin C as references. The tests revealed the following order of antioxidant activities, ethyl acetate fraction>extract>water fraction>n-hexane fraction. All test substances had IC50 of <50 ppm, which categorized them as having very high antioxidant activities. In line with this data, on the basis of reducing power, ethyl acetate extract was shown to be the most potent antioxidant, having a value of 20.24 mgAAE/g sample. Overall, results of the present study suggest the potential use of black grass jelly as a part of the therapeutic armamentarium for oxidative stress-related diseases.

The Use of Natural Polymers to Enhance Oil Recovery

This paper reports a laboratory study on the use of natural polymers to increase oil recovery. The use of natural polymers compared to synthetic polymers is more economical due to their abundant availability, renewability, biodegradability, and non-toxicity to the environment as they do not use chemicals. The purpose of this research is to measure the aqueous solution stability test and recovery factor produced by natural polymers through core flooding tests. The natural polymers used are Shrimp Chitosan, Crab Chitosan, Cyclea Barbata Miers, Mesona Palustris, and Seaweed. The methods used in this research are aqueous solution stability test and core flooding test. The shrimp chitosan solution used had a concentration from 5,000; 10,000 and 15,000 ppm and 2 salinities between 10,000 and 20,000 ppm. The crab chitosan solution used had a concentration from 5,000; 10,000 and 15,000 ppm with 2 salinities between 10,000 and 20,000 ppm. The Cyclea barbata Miers solution used had a concentration from 1,500; 2,500 and 3,500 ppm and 2 salinities between 7,000 and 15,000 ppm. The Mesona Palustris solution used had a concentration from 500; 1,000 and 2,000 ppm and 3 salinities from 10,000; 15,000 and 20,000 ppm. The Seaweed solution used had a concentration from 1,000; 2,000 and 3,000 ppm and 3 salinities from 5,000; 10,000 and 15,000 ppm. Sandpack is used as a porous media for crab chitosan experiments, while sandstone core is used for Cyclea Barbata Miers and Mesona Palustris with crude oil 44°API. The aqueous solution stability test showed that all polymer solution samples were clear for various concentrations and salinities. Except for the solutions of Cyclea barbata Miers and Masona palustris which are greenish and blackish respectively. Transmittance for shrimp and crab chitosan solutions shows a value of more than 80%. This shows that natural polymers are compatible with brine. Flooding tests were carried out to measure oil recovery related to the use of natural polymers. The experimental results showed that the best additional oil recovery was 10.3% using crab chitosan solution injection. The chitosan solution used had a concentration of 15,000 ppm and a viscosity of 1,729 cp. We can conclude that the addition of polymer can improve recovery factor.

Mesona palustris BL: the potential antioxidant

Mesona palustris, also known as ‘Black Cincau’ was a plant that was commonly found in East and South East Asian countries. Recently, interest in these Mesona palustris components, such as antioxidants. It can reduce the damage in the cellular aspect either by quenching free radicals or through the chelation of transition metal ions, which reduces their capability to form reactive oxygen species. M.palutris can be composed of more nutraceutical options, including edible jellies. Some previous studies investigated the effective and beneficial extraction process of M. palustris to elucidate the chemical constituents of M.palustris. This plant is also known to have some phenolic compounds, such as flavonoids and tannins. Nowadays, it has been reported to yield many thrilling studies as an antioxidant.

Beneficial Effect of Mesona palustris BL: A Review on Human and Animal Intervention

BACKGROUND: Black Cincau (Mesona palustris BL) has been used widely as a complementary and alternative therapy in East and Southeast Asian regions due to its potent antioxidant activity and proven health benefits. It is traditionally used to treat various diseases such as antihypertensive, fever, and anti-diarrhea. Some studies to explore the function of Black Cincau were carried out on animal and humans, but publications regarding, it are still limited. AIM: This review emphasizes the beneficial effect of M. palustris BL. In addition, this review corroborates the pharmacological basis for the various health benefits, traditional, and medicinal applications of M. palustris BL. METHODS: All the available information of Black Cincau (M. palustris BL) was collected from electronic databases such as PubMed, Google Scholar, and ProQuest from 2011 to 2020. This literature review suggests that M. palustris BL has a beneficial effect. All Black Cincau (M. palustris BL) available information was collected from electronic databases such as PubMed, Google Scholar, and ProQuest from 2011 to 2020. RESULT: This literature review suggested that M. palustris BL has a beneficial effect. Researchers have proven in preclinical models that M. palustris BL is pharmacologically active as an antioxidant and can reduce cholesterol, blood glucose, immunomodulatory, and gastrointestinal healthy. CONCLUSION: Several preclinical studies on M. palustris BL have been carried out, but the application of the effectiveness of M. palustris BL in humans still needs to be developed to provide the pharmacological basis for the various health benefits, traditional, and medicinal applications of M. palustris BL.

Productivity Development and Marketing of Janggelan, Mesona palustris in Jeruk Village, Bandar District, Pacitan Regency

Janggelan ( Mesona palustris BL) is usually used for making black grass jelly, but can also be used for cosmetic ingredients. Increasing production through intensification programs requires farmers' understanding of the problem of janggelan. Problems are not only limited to cultivation techniques, but also include problems with the introduction of plants, their uses, nutritional value and market potential. The research objective was to determine the potential of janggelan and to analyze the janggelan farming and marketing channels in Jeruk Village, Bandar District, Pacitan Regency. This research uses analytical methods and its implementation with survey techniques. The method of determining the research area used purposive sampling method, namely in Jeruk Village, Bandar District, Pacitan Regency. Determination of the number of samples using the random sampling method. The data used are primary data and secondary data. The data collection technique was done by taking notes, observing and interviewing. The results showed that the productivity of Janggelan plants in Jeruk Village, Bandar District, Pacitan Regency, has the potential to develop Janggelan plants ( Mesona palustris BL) and there are 4 (four) marketing channels carried out to market Janggelan plants ( Mesona palustris BL).

Extraction optimization for antioxidant phenolic compounds in black grass jelly (Mesona palustris BL) using response surface methodology

Black grass jelly is a traditional plant that is more widely used as medicine to prevent carcinogenesis, diabetes, and hypertension, to overcome diarhea, because it is antimutanogenetics, stabilizers, and immunomudulators, besides black grass jelly also contains antioxidants. This study examines changes of temperature and solvent ratio toward the composition of phenols, tannins, and flavonoids which are antioxidant constituents. Analysis of phenols and tannins uses folin quality reagents, while analysis of flavonoids uses the spectrophotometer method. The change of temperature and solvent ratio gives effect to change of phenol, tannins, and flavonoids amount. Optimal extraction of phenol, tannin and flavonoids using the response surface methodology (RSM) occur at a temperature range of 94-100°C with a solven ratio of 23-24 ml/g.

IDENTIFIKASI ES CHERICHIA COLI PADA CINCAU YANG DIJUAL DI PASAR BARU STABAT

Escherichia coli ditemukan di usus manusia dan hewan. Bakteri ini mudah menyebar dengan mencemari air dan makanan. itu bisa terjadi pada cincau (Mesona palustris) yang memiliki karbohidrat, serat dan nutrisi yang tinggi. Sebagian orang menggunakan campuran minuman cincau dan juga obat tradisional. Penelitian ini bertujuan untuk mengidentifikasi kontaminasi bakteri E. coli cincau yang dijual di Pasar Baru Stabat. Sampel dalam penelitian ini adalah cincau yang berasal dari 5 pedagang. Penelitian ini merupakan penelitian deskriptif. Metode penelitian adalah MPN (Most Probable Number) untuk menghitung bakteri coliform, diikuti dengan pemeriksaan media Lactosa Broth, Brilliant Green Lactose Broth media, media Eosin Metilyene Blue Agar, pewarnaan gram dan tes Biokimia. Hasil penelitian ini menunjukkan lima sampel yang diuji, ada satu sampel positif yang terkontaminasi dengan bakteri E. Coli dan 4 sampel negatif yang terkontaminasi E. coli. Sehingga empat sampel cocok untuk dikonsumsi dan satu sampel tidak sesuai untuk konsumsi.

Nata de cincau: Pacitan’s black cincau (Mesona palustris BL) product innovation with respect to its physical, chemical and sensory characteristics

Black cincau (Mesona palustris BL) is a particularly abundant commodity in Pacitan, Indonesia. It has a higher fibre and antioxidant content compared to other varieties. Black cincau is potentially processed into nata de cincau in order to diversify food products in Pacitan. The aim of this study is to define the best formulation of nata de cincau, considering its physical (yield, thickness and texture) and chemical (water and crude fibre) characteristics. The best three formulations, based on physical and chemical characteristics, were chosen for sensory evaluation (colour, flavour, taste, texture and overall). Nata de cincau processing used various concentration of substrates (extract black cincau) (2.5%, 7.5% and 12.5%) and a variety of starter concentrations (5%, 10% and 15%) were added. Nata de cincau with 12.5% substrate concentration and 5%, 10% and 15% starter concentration were the best three formulations in terms of all physical and chemical characteristics. Sensory evaluation was performed on the best three formulations; 12.5% substrate concentration and 15% starter concentration was found to be the best formulation.

Land suitability evaluation for grass jelly (Mesona palustris BI.) and land conservation in Nawangan, Pacitan Regency

One of land degradation problems in Nawangan is surface erosion, throughout the years it widespread. Land conservation by planting plantations in the potential disturbed area is one way to solve this problem. Grass jelly or locally known as janggelan ( Mesona palustris BI. ) can be an alternative for this land conservation, Nawangan has suitable geographic location and grass jelly cultivation is profitable for the surroundings economically. Aim of this study was to evaluate land suitability for grass jelly to solve the land degradation problem in Nawangan. Purposive sampling methods were used to determine sample point, then soil sample analyzed in a laboratory, and overlay of type of soil map and land use map. After soil characteristic was obtained, then the matching process was used. Finally, from this study showed that land suitability classes in Nawangan for grass jelly are very suitable on (S1) land unit LaS and LaK, suitable (S2) on land unit Lil, LiH, LiS and LaH, and marginal suitable (S3) on land unit LaL and LiK.

Pemberdayaan Masyarakat Kecamatan Nawangan, Kabupaten Pacitan Melalui Penganekaragaman Olahan Daun Cincau Hitam

Black cincau leaf (Mesona palustris BL) is one of the main commodities of Nawangan District, Pacitan Regency which has high fiber and antioxidant content. In general, the cincau leaves produced at Nawangan are only sold in the form of dried leaves so that their economic value is low. Processing of dried black grass jelly leaves into processed food products can increase its economic value. The problem faced by the Nawangan community in an effort to increase the economic value of cincau leaves is that the Nawangan people do not yet have the skills and knowledge regarding various forms of processed which have a higher selling value. Through the Community Empowerment Community Service program, efforts are made to diversify processed products of black cincau leaves in the form of pastry products (nastar and cookies). This program aims to improve the skills of the Nawangan community in processing black cincau leaves into several food products that have high selling value. This activity was carried out in three stages, namely socialization, implementation of training and monitoring. The outputs produced in this activity are processed products of black cincau leaves which are expected to be the superior products of the Nawangan District.

Anti-inflammatory effect of Black cincau (Mesona palustris BL) based supplement in male carrageenan-induced mice

This study aims to investigate the potency and optimum dose of Mesona palustris supplement as an anti-inflammatory in carrageenan-induced mice (Musmus culus). In this study, twenty male Balb/C mice were divided into five groups. The treatment groups are including a negative control, positive control, drug control, and two different doses of black cincau based herbal supplement (BCBHS) groups. In this experiment, edema was observed surrounding mice feet, and the relative number of NF-α and CD68 were measured using flow cytometry analysis. The results revealed that both of BCBHS doses were effective to inhibit edema incidence in carrageenan-induced mice. The expression level of TNF-α and CD68 in both of BCBHS doses were lower than the positive control. Further, the tissue recovery was observed with M. palustris supplementation. Our result suggests that both of BCBHS doses have an anti-inflammatory effect in carrageenan-induced mice.

Black Grass Jelly (Mesona Palustris Bl) Effervescent Powder has Anti-Dyslipidemia in High Cholesterol Diet-Fed Rats and Antioxidant Activity

Dyslipidemia that could be affected by hypercholesterol diet is a contributing factor in millions of deaths worldwide and a risk factor for ischemic heart disease and stroke. High soluble fiber diet is used as one of the non-pharmacological treatments to control hypercholesterolemia. Black Grass Jelly (Mesona palustris BL) also known as black cincau has a high soluble fiber content. This study aimed to investigate the effect of black cincau as effervescent powder form (BCEP) for anti-dyslipidemia and also the antioxidant activity. BCEP was orally administered to twenty male dyslipidemia Wistar rats induced with hypercholesterol diet for 3 weeks then plasma lipid profile was measured using enzymatic CHOD-PAP assay. The antioxidant activity also has been identified. Oral administration of 3 different doses of BCEP resulted in lipid profiles by decreasing plasma cholesterol (TC), triacylglycerol (TG), low-density lipoprotein (LDL), and also high-density lipoprotein (HDL) in dyslipidemia rats (α = 0.01). The increasing doses of BCEP revealed that it has a potential effect of decreasing plasma lipid.

IMMUNOMODULATORY EFFECTS OF BLACK CINCAU (MESONA PALUSTRIS BL.) SUPPLEMENT ON ESCHERICHIA COLI STRAIN O157-INFECTED MICE

Objective: This study objective was to determine the immunomodulatory effects of the black cincau (Mesona palustris BL.) supplement on Escherichia coli strain O157-infected mice.Methods: Black cincau (Mesona palustris BL.) and red ginger (Zingiber officinale var. rubrum) were extracted on a pilot plant scale. For the animal experiment, the mice were adapted for 7 days with black cincau supplement at a dose of 43.29 mg/kg b.w. (SSP I), a dose of 85.58 mg/kg b.w. (SSP II), a dose of 4.33 mg/kg b.w. of a commercial immunomodulator (SK) and buffer saline for the negative control (KN) and positive control (KP). Then, mice were infected intraperitoneally (intraperitoneally) with E. coli strain O157 1010 cfu/mL, except KN. On day 21, the cell of mouse spleen was analyzed using flow cytometry.Results: Result showed that administration of black cincau supplement up to 85.58 mg/kg b.w gave immunomodulator effects in infected mice. Immunomodulator effect can be seen through the increasing of the relative average of a cluster of differentiation (CD)4+ T cells, CD8+ T cells, CD4+ CD8+ T cells, CD4+ CD62L T cells, CD8+CD62L T cells, and CD68+interferonγ (Interferon gamma) monocytes cells. Exposure of black cincau supplement on infected mice can recover small intestine mucosa structure of mice.Conclusions: This study demonstrates that the supplement of black cincau extract can give an immunomodulatory effect on E. coli-infected mice.

Antihypertensive Effects of Black Cincau (Mesona palustris BL) Effervescent Powder and Malondialdehyde Concentration on Wistar Rats as a Hypertensive Model

This study investigated the effects of Black cincau ( Mesona palustris BL ) effervescent powder (BCEP) on systolic blood pressure and malondialdehyde concentration in hypertensive rats. BCEP were orally administered to hypertensive rats for 4 weeks, and antihypertensive effects were determined. Rats were given 630 mg/kg, 1.260 mg/kg and 1.890 mg/kg BCEP on dose group 1, 2, 3 respectively. These groups were compared with a negative control group, hypertensive control group and Captopril control group. These results showed that systolic blood pressure and MDA concentrations of rats dosed with BCEP were significantly different (α=0,01) to those of control rats. Rats dose third group with of 1.260 mg/kg showed the highest percentage of lowering blood pressure. The rat dose third group had highest percentage decreased in systolic blood pressure (43,97%) and in MDA concentration (68,77%). Key words : Antihypertensive ; Black Cincau ( Mesona palustris BL ) Effervescent Powder; Malondialdehyde

Technology Of Black Cincau Liang Tea As Diversified Products In Black Cincau Gel Producer At Batu City

ABSTRACT Black cincau (Mesona palustris BL) or black grass jelly is traditional beverage ingredient that is believed has efficacious for health, but the black cincau gel in the market known asunhygienic food and has shortshelf life. Liang tea of black cincau is a kind of processed product development of black cincau. Liang tea or herbal tea made from of black cincau extracts with the addition of other herbs such as ginger, cinnamon or pandan leaves. Sumber Murni home industry is theproducerof blackcincaugelinBatucitywhich isinterested inproducingliang tea of black cincau in a plastic cup packaging as well as product diversification for enterprise development. Therefore, Liang tea of black cincau technology package should be a well proven technology where the technology is practical, easy to introduce. Technology which applied are: 1 ) Formulation of Liang Tea is black cincau extract : water = 60 : 40 and ginger extract added = 10 % , 2 ) The process is suitable with the flow diagram, 3 ) Engineering equipment such as semi-automatic cup sealer with tank and cookers tank for blending materials with automatic stirrer . Keywords: cookers tank with automatic stirrer,liang tea of black cincau, semi-automatic cup sealer

Hepatoprotective Effect of Extract of Black Cincau (<em>Mesona palustris</em> BL) on Paracetamol-Induced Liver Toxicity in Rats

Black cincau (<em>Mesona palustris</em> BL) or Grass black jelly is a traditional Indonesian food that has been used as a folk medicine and is effective against heat-shock, hypertension and diabetes. Therefore, the aim of this research was to determine the hepatoprotector effects of ethanol extracts of black cincau in protecting the liver induced by high doses of paracetamol. The ethanol extracts of black cincau were studied for their hepatoprotective and antioxidant effects on paracetamol (750 mg/kg BW) induced acute liver damage on Wistar rats and treatment during 4 weeks. The degree of protection was measured by using biochemical parameters such as Serum Glutamate Oxalate Transaminase (SGOT) and Serum Glutamate Pyruvate Transaminase (SGPT) and Alkaline Phosphatase (ALP) were estimated. The result showed that ethanol extracts of black cincau IC₅₀ value of 46.92 ppm and 829.7 ppm for total phenols. The ethanol extracts of black cincau at a dose level of 500 and 1000 mg/kg BW produce significant (p<0.05) hepatoprotection by decreasing the activity of SGOT, SGPT and ALP). From this study, it can be concluded that the ethanol extracts of black cincau is not only an effective hepatoprotective agent, but also possesses significant (p<0.05) antioxidant activity.

Cytotoxic Effect of Water, Ethanol and Ethyl Acetate Extract of Black Cincau (Mesona Palustris BL) Against HeLa Cell Culture

Black cincau (Mesona palustris BL) or grass black jelly as a traditional Indonesian food that has been used as a folk medicine and as a beverage for many centuries. It was reported that some of pharmaceutical effect of herbs could be related to their possible antioxidant activities. Moreover, some researchers reported that diverse compounds naturally occurring in food could exert an antimutagenic effect due to their antioxidant capacity. The studies to determine the bioactivity of black cincau extract against Hela cell. Cytotoxicity assays was conducted using method the MTT with 10 levels of extract concentration. The results of cytotoxicity assays showed the water extract, ethanol extract and the ethyl acetate extract of black cincau has anticancer activity against Hela cell lines. The third activity of black cincau extracts was significantly different. Water extracts of black cincau at low concentrations (19.53 - 156,25μg/ml) showed higher anticancer activity than extracts ethanol and ethyl acetate. IC50 water extract of black cincau was 132,6μg/ml, ethanol extract of black cincau was 146,0μg/ml and ethyl acetate extract of black cincau was 182,96μg/ml.