Meniran Extract Research Articles

IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS EKSTRAK ETANOL HERBA MENIRAN (Phyllanthus niruri L.) TERHADAP CANDIDA ALBICANS MENGGUNAKAN METODE DIFUSI CAKRAM

The aim of this research is to know the ability of meniran ethanol extract (Phyllanthus niruri L.) in inhibiting the growth of Candida albicans. The research design is an experimental study. The extract used was herbal meniran extract made by maceration using 70% ethanol solvent, the content of ethanol extract of meniran tested against Candida albicans using disc diffusion method were 5%, 10%, 20%, 30% and 40%. Activity test conducted on Sabaraound Dextrose agar media that has been smeared with Candida albicans culture which has been standardized with Mc Farland standard. The pre-tested disc paper is first dipped into the extract with various concentrations. Incubated at 30 ° C for 24 hours and the inhibit zone formed was measured. The result showed that herbal ethanol extract of meniran resulted in drag zone diameter at Candida albicans ie 8,5 mm; 10.3 mm; 12.6 mm; 14.1 mm and 14.3 mm, for negative control 0 mm and positive control 20.1 mm. Data obtained from the results of the study were analyzed using one way anova method showed p - value <0,05, it was concluded that there was significant difference between negative control, 5%, 10%, 20%, 30%, 40% and positive control.

Efek Ekstrak Campuran Kulit Batang Pulai (Alstonia scholaris) dan Meniran (Phyllanthus niruri) pada Mencit Swiss Webster yang Diinfeksi Plasmodium berghei

Pulai and meniran is one of the medicinal plants that have been studied as a possible antimalarial drugs. This study aims to evaluate the antimalarial activity of a mixture of pulai bark and meniran extracts as antimalarial drugs. Acute toxicity tests was performed using male and female Wistar rats each 25 animals with four doses of treatment. Antimalarial test using 72 mice were divided into six dose groups: group CMC, DHP, dose mixture of 1330; 443.34; 147.78 mg/kg bw and doses of pulai groups 1330 mg /kg bw. All the mice were infected with Plasmodium berghei (D0) and given the extracts orally for 14 days. Giemsa blood stainning taken on days D1-D7 and D14 were analyzed for percentage of parasitaemia, lymphocytes, monocytes and granulocytes. Results of acute toxicity test (LD50) values obtained from extract mixture was more than 14285 mg/kg bw, are classified as non-toxic materials. The most effective dose of the test antimalarial obtained from the percentage of parasites reducing and leukocytes differential, was 147.78 mg/kg. A mixture of pulai bark and meniran extract can be considered to be used as an alternative drug in the treatment of malaria. Further research is needed to isolate and characterized the active ingredients which have the effect of antimalarial to be recommended as an antimalarial drug in the future.

HEPATOPROTECTIVE ACTIVITY OF SAPONIN FRACTION OF OYONG SEED FLESH AND ITS COMBINATION AGAINST CCl4-INDUCED CHRONIC LIVER DAMAGE IN MALE WISTAR RAT

Saponin fraction of seed flesh of Oyong (Luffaacutangula [L.]Roxb) has been investigated to have a hepatoprotective activity in rats with fibrotic chronic liver damage. This research was conducted to evaluate whether saponin fraction of Oyongseed flesh has a hepatoprotective activity in CCl4-induced acute liver damage. Hepatoprotective activity was determined by measuring the activity of liver enzymes (SGOT, SGPT, LDH), total nitrite/nitrate level, liver index and liver histology. Saponin fraction of Oyong flesh seeds 10mg/kg BW and meniran extract 400mg/kg BW alone showed hepatoprotective activity. Administration of saponin fraction 10mg/kb BB decreased SGPT and LDH significantly over untreated group. Group given meniran extract at dose of 200mg/kg BW showed decreased on LDH, while at dose of 400mg/kg BW decreased SGPT, SGOT, and LDH significantly. Hystological observation revealed any improvement in liver morphology especially after treated with saponin fraction 10mg/kg BWand meniran extract at dose of 400mg/kg BW. However, all groups treated with combination of saponin and meniran did not show improvement both at biochemical parameter and liver histology. In conclusion, saponin extract with dose of 10mg/kg BW and meniran extract at dose of 400mg/kg BW showed hepatoprotector activity. In contrast, combination of both did not show any hepatoprotective effect and it was suspected that they have antagonist effects. Key words:hepatoprotective, CCl4-induced liver fibrosis, Luffaacutangula, Phyllanthusniruri

MINUMAN TINGGI AKTIVITAS ANTIOKSIDAN BERBAHAN DASAR ALAMI MINYAK BEKATUL PADI (Oryzae sativa) DAN EKSTRAK MENIRAN (Phyllanthus niruri)

<p>This study aimed to assess the antioxidant potential of chocholate-rice bran oil-emulsion beverage and meniran extract beverage. RBO beverage was made using oil in water emulsion with sugar ester as the emulsifier.Four formulas was created with the RBO-chocolate concentrate : water ratio of 1:1, 1:3, 1:5, and 1:7. Meanwhile the meniran beverage was made by adding extract meniran in five different concentrations (30, 40, 50, 60, 70 ppm) along with flavor and sucralose. The selected emulsion beverage had the AEAC value of 30.75 mg Vit C/100 g, while the meniran extract beverage had the AEAC value of 9.12 mg Vit C/100 g with the total fenol of 92.75 mg/100 g. The results of TBA value, content of protein, fat, carbohydrate, ash, and water of the emulsion beverage were 0.12 mg malondialdehyde eq/kg, 0.24%, 0.52%,6.74%, 0.15% and 92.32%, respectively. Increasing concentrations of chocolate and RBO significantly affected the results of the chemical parameters (p<0.05). Overall, the chocolate emulsion beverage based on RBO and meniran extract beverage had high antioxidant activity that are potential to prevent non-communicable diseases.</p>

The effect of meniran (Phyllanthus niruri) extract protection on mice gastric histology damage induced by aspirin

Rachmawati P, Suparyanti EL, Isdariyanto. 2010. The effect of meniran (Phyllanthus niruri) extract protection on mice gastric histology damage induced by aspirin. Biofarmasi 8: 41-46. This experiment aimed to determine the effect of meniran (Phyllanthus niruri Linn.) extract protection on mice (Mus musculus) gastric histology damage induced by aspirin. This study belongs to an experimental laboratory research with post-test only controlled group design. The tested animals used were 25 Swiss Webster male mice in 6-8 weeks age and ±20 g body weight, divided into 5 groups: (i) control group (K) that given with 0.2 mL CMC, Na 0.5% and 0.1 mL aquadest, (ii) treatment group I (P1) that given with 0.2 mL aquadest and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, (iii) treatment group II (PII) that given with 0.2 mL meniran extract with a dosage of 1.3 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, (iv) treatment group III (PIII) that given with 0.2 mL meniran extract with a dosage of 2.6 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, and (v) a positive control group (PIV) that given with 0.2 Cimetidine with a dosage of 2.6 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral. The gastric histological description could be seen with a bright light microscope with 100x magnification followed by 400x magnification. Then, the gastric histological description was divided into normal, light damage, and heavy damage. The data obtained were analyzed by using Kruskal Wallis and Mann Whitney statistical tests at α = 0.05. The results of Kruskal Wallis statistical test showed that there was at least one population with a value that higher than other population. The result of Mann Whitney statistical test showed that there was a significant difference between groups K-PI, PI-PII, PI-PIII, and PI-PIV, and no significant difference between K-PII, K-PIII, K-PIV, PII-PIII, PII-PIV, and PIII-PIV. The conclusion of the research was the meniran (Phyllanthus niruri Linn.) extract can give a protection on mice (Mus musculus) gastric histological damage induced by aspirin.