Membranotropic Effect Research Articles

Membranotropic effect of low-intensity laser radiation of the blood

We have examined the effect of a low-intensity laser radiation (wavelength 630 nm, beam power 1 mW) on the aggregability of blood platelets, acidic tolerance of erythrocytes, and the structure of their membranes. Laser radiation suppressed platelet aggregability induced by ATP, epinephrine, collagen, platelet activating factor, and fibrinogen and enhanced the resistance of the erythrocytes. It decreased the microviscosity of lipid bilayer and of the membrane protein-lipid contact regions and slowed down lipid peroxidation. These findings attest to a direct membranotropic effect of laser radiation, which is particularly strong in the protein-lipid contact regions.

Tamm-Horsfall glycoprotein (THG) purified from normal human pregnancy urine increases phagocytosis, complement receptor expressions and arachidonic acid metabolism of polymorphonuclear neutrophils

Tamm-Horsfall glycoprotein (THG) purified from normal human pregnancy urine was found to increase polymorphonuclear neutrophil (PMN) phagocytosis (46.57 ± 3.54% in the medium versus 75.85 ± 5.37% in the presence of 25 μg/ml THG) after 30 min preincubation. The phagocytosis-enhancing activity of THG was dose-dependent (5–50 μg/ml) and was possibly mediated by the increased expressions of complement receptor type 1 (CR1) and type 3 (CR3) on the neutrophils. The release of [3H]arachidonic acid and prostaglandin E2 (PGE2), but not thromboxane B2 (TXB2), from neutrophils were also significantly enhanced by THG. Using 3,3′-dihexyloxacarbocyanine iodide as indicator, THG (25 μg/ml) depolarized the membrane potential of PMN after 30 min preincubation. In addition, THG exhibited a specific membranotropic effect with PMN. It is conceivable that THG binds to the cell surface and depolarizes the membrane potential of PMN which subsequently enhances the release of arachidonic acid metabolites and the translocation of the complement receptors to the membrane. These biochemical events lead to the increment of PMN phagocytosis and suggests that THG may play an important role in the defense mechanisms of the urinary tract in that a large amount of THG is usually present.

Effect of γ-butyrolactone derivative on an artificial membrane

trans-2-(6'Methylheptanol-1'-yl)-3-hydroxymethyl-4-butanolide(II) was shown to increase the passive flux of cations such as Co 2+ through a black lipid membrane made from ox brain phospholipids. This membranotropic effect appears to be involved in the activity of II towards blocked mutants of streptomycetes as an autoregulator of cytodifferentiation.

Effect of regeneration stimulators on cellular and intracellular organelle function in the early stages of the membranotropic effect of environmental chemical factors

Effect of regeneration stimulators on cellular and intracellular organelle function in the early stages of the membranotropic effect of environmental chemical factors

Effect of trasylol on permeability of the tissue-blood barriers of the ischemic myocardium

Ligation of the descending branch of the left coronary artery caused an increase in the permeability of the tissue-blood barriers of the myocardium in the ischemic zone and in the boundary zone for sulfacetamide sodium. Trasylol reduced the permeability of the myocardial tissue-blood barriers in these zones and did not affect permeability in the intact zone. The use of a fluorescein test revealed the presence of membranotropic effect of trasylol; the compound did not affect the osmotic resistance of the erythrocytesin vitro.