Medium Formulation Research Articles

Kultur Embrio Tiga Spesies Kopi pada Umur Buah dan Formulasi Media yang Berbeda

<em>Studying the fruit age and proper media formulation is one of the important stages in embryo culture of coffee. The data is highly benefical, especially in saving embryos generated from intra- and inter-species crosses that fall prematurely or experience problems in germination. The aim of this study was to determine the suitable age and media formulation for embryo culture of Arabica, Robusta, and Liberica coffee. The study was conducted at the Tissue Culture Laboratory, Indonesian Industrial and Beverage Crops Research Institute from January 2019 to November 2020. Murashige and Skoog (MS) media with growth regulators adapted to embryonic development were used in this study. The three types of coffee divided into 5 groups, namely pinhead, immature, early mature, almost mature, mature, and used as planting material. The research was designed in a completely randomized design with 10 replications, and media formulation as a treatment. The results showed that embryo culture of the three coffee species was conducted successfully, except for pinhead fruit. The older the cultured fruit, the higher the percentage of germination. There is a difference in germination time between the three coffee species. The medium for embryo culture should be adjusted with the age of the fruit being cultured. Aside from growing embryos, the cultured mature fruit embryos on MS medium given 0.5 mg/l BA can also be used for propagation by utilizing the secondary somatic embryos formation.</em>

Embryo Culture of Three Coffee Species on Various Fruit Ages and Media Formulations

Mempelajari tahapan umur buah dan formulasi media yang tepat menjadi salah satu tahapan penting dalam kultur embrio. Data ini sangat bermanfaat terutama ketika kita akan melakukan penyelamatan embrio hasil persilangan intra (dalam) dan inter (antar) spesies kopi yang gugur sebelum waktunya atau mengalami kendala dalam perkecambahan. Penelitian bertujuan untuk mengetahui umur dan formulasi media yang sesuai pada kultur embrio kopi Arabika, Robusta, dan Liberika. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Industri dan Penyegar mulai Januari 2019 sampai November 2020. Media Murasige and Skoog (MS) dengan zat pengatur tumbuh yang disesuaikan dengan perkembangan embrio digunakan dalam penelitian ini. Tiga jenis kopi dikelompokkan ke dalam 5 kelompok, yaitu: mata yuyu, belum matang, awal mulai matang, hampir matang, matang, dan dijadikan bahan tanam. Rancangan penelitian menggunakan rancangan acak lengkap dengan 10 ulangan dengan formulasi media sebagai perlakuan. Hasil penelitian memperlihatkan kultur embrio ketiga spesies kopi berhasil dilakukan, kecuali pada buah mata yuyu. Semakin tua umur buah yang dikulturkan, persentase perkecambahan semakin tinggi. Terdapat perbedaan waktu berkecambah antara ketiga spesies kopi. Media terbaik untuk kultur embrio disesuaikan dengan umur buah yang dikulturkan. Selain menumbuhkan embrio, kultur embrio buah matang pada media MS yang diberi BA 0,5 mg/l juga dapat digunakan untuk perbanyakan dengan memanfaatkan pembentukan embrio somatik sekunder.

PEMANFAATAN PEKARANGAN UNTUK BUDIDAYA ANGGUR DENGAN MEDIA CAMPURAN BIOCHAR

The Covid-19 pandemic has significant socio-economic impacts for all regions in Indonesia, one of which is in Bantul Regency. In anticipation of this impact, Mayungan Hamlet government officials and the community service team made several programs related to the development of grape cultivation in their home yard. With this activity, it is hoped that the community will be able to improve the family economy which was originally declining. The series of activities carried out were mapping of village potentials, outreach, practice, mentoring, and technical guidance. This activity was chosen in order to make it easier for the community to accept new technology with the formulation of planting media made from compost and coconut shell biochar. The obstacle faced during the service was that all activities were carried out during a pandemic where there were restrictions on community activities so that not all participated in this activity. However, all activities were successfully carried out, as evidenced by the 14 invited community representatives, all of whom were present in every activity and there was an increase in community (cognitive) knowledge about grape cultivation.

Can Culture Media Impact Preimplantation Embryo Aneuploidy?

With continued improvements in blastocyst culture, cell sampling approaches, and genetic analysis platforms, the resulting improvements in embryo development and the resolution and accuracy of chromosome analysis have provided valuable insights into the preimplantation embryo. This includes the impact of in vitro culture conditions on chromosomal dynamics. Specifically, through analysis of embryo aneuploidy and mosaicism, a growing number of reports indicate that rates of chromosomal abnormalities can vary between IVF centers. Because differences in mosaicism reflect mitotic errors, this endpoint analysis suggests that IVF laboratory-controlled variables during embryo development may be influencing chromosome separation and segregation. A growing body of literature suggests that culture media may be one variable influencing preimplantation embryo aneuploidy and mosaicism. However, these data are far from definitive in demonstrating cause-and-effect. Whether reported differences may be due to media formulation, use of sequential media or single-step media, or uninterrupted culture approaches is unknown. Importantly, variables directly impacting media performance and embryo development, including pH, temperature, osmolality, and oxygen concentration, must also be considered and make it difficult to isolate the impact of culture media as the sole factor responsible. These IVF laboratory variables will be reviewed and literature suggesting a possible link to mitotic aneuploidy/mosaicism will be discussed.

The Combination of Untargeted Metabolomics and Machine Learning Predicts the Biosynthesis of Phenolic Compounds in Bryophyllum Medicinal Plants (Genus Kalanchoe).

Phenolic compounds constitute an important family of natural bioactive compounds responsible for the medicinal properties attributed to Bryophyllum plants (genus Kalanchoe, Crassulaceae), but their production by these medicinal plants has not been characterized to date. In this work, a combinatorial approach including plant tissue culture, untargeted metabolomics, and machine learning is proposed to unravel the critical factors behind the biosynthesis of phenolic compounds in these species. The untargeted metabolomics revealed 485 annotated compounds that were produced by three Bryophyllum species cultured in vitro in a genotype and organ-dependent manner. Neurofuzzy logic (NFL) predictive models assessed the significant influence of genotypes and organs and identified the key nutrients from culture media formulations involved in phenolic compound biosynthesis. Sulfate played a critical role in tyrosol and lignan biosynthesis, copper in phenolic acid biosynthesis, calcium in stilbene biosynthesis, and magnesium in flavanol biosynthesis. Flavonol and anthocyanin biosynthesis was not significantly affected by mineral components. As a result, a predictive biosynthetic model for all the Bryophyllum genotypes was proposed. The combination of untargeted metabolomics with machine learning provided a robust approach to achieve the phytochemical characterization of the previously unexplored species belonging to the Bryophyllum subgenus, facilitating their biotechnological exploitation as a promising source of bioactive compounds.

Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga Crypthecodinium cohnii

Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from a starch industry, were used as carbon and nitrogen sources, respectively, for lipid production, using the heterotrophic microalga C. cohnii grown in a bench bioreactor, in a batch culture. The maximum biomass concentration, lipid content and lipid productivity attained were 5.34 g/L, 24.6% (w/w Dry Cell Weight-DCW) and 0.016 g L−1 h−1, respectively. Flow cytometry analysis was used to evaluate the impact of these substrates on the microalgae cells. A high proportion of intact cells with enzymatic (esterases) activity (>50%) was present throughout the cultivation time course. These results indicate that crude glycerol and CSL can be used in the medium formulation for DHA and lipid production using this microalga, which reduce the process costs in an expected maximum of 84%.

Optimization of fermentation medium for succinic acid production using Basfia succiniciproducens

The development of cost-competitive bioprocesses is dependent on the formulation of low cost and efficient fermentation medium. Thus, optimization of nutrient composition in fermentation media combining efficient metabolic product formation with cost-competitiveness is needed. Within this context, the main novelty of this study was to optimize the nutrient composition of a fermentation medium used for bio-based succinic acid production by the wild-type bacterial strain Basfia succiniciproducens JF4016 combining low cost with high succinic acid production efficiency. Two Plackett–Burman​ Designs, a central composite design and constraint optimization were sequentially implemented to estimate the optimum and cost-competitive fermentation medium composition. The two Plackett–Burman Designs identified NaH 2 PO 4 , Na 2 HPO 4 and NaCl as the most important medium components. Central composite design identified the optimum concentrations of NaH 2 PO 4 , Na 2 HPO 4 , NaCl, yeast extract and corn steep liquor leading to the highest productivity. Constraint optimization was employed to maximize productivity using constraints on the final succinic acid concentration, yield, by-products to succinic acid ratio and fermentation medium cost. Validation of the model predictions was carried out in a bioreactor culture at ca. 30 g/L initial glucose concentration. The optimum fermentation medium containing 19.4 g/L CSL, 9.2 g/L yeast extract, 3 g/L NaCl and 4.32 g/L sodium phosphates led to the production of 19 g/L succinic acid concentration, 0.62 g/g yield and 1.9 g/(L ⋅ h) productivity. • Experimental design to optimize fermentation medium for succinic acid production by Basfia succiniciproducens . • Succinic acid production reached 19 g/L with 0.62 g/g yield and 1.9 g/(L⋅h) productivity. • Reduced fermentation medium cost ($1.22/kg SA ) was estimated at the optimal medium composition.

Development of novel media formulation using rice straw and corn steep liquor for pyruvate production

Development of novel media formulation using rice straw and corn steep liquor for pyruvate production

Screening of Agricultural Wastes for Substrates in Oxalic Acid Production Using Aspergillus niger

The disposal and attendant problems associated with agro-wastes have remained a challenge to the environment. Three agricultural wastes (cassava whey, banana peels and groundnut shells) were collected from the Choba and Yam zone markets in Rivers State, Nigeria and screened for their potential as substrates in the formulation of fermentation media to produce oxalic acid. The inoculum for the study was isolated from the banana peels and identified using the megablast search for highly similar sequences from the NCBI non-redundant nucleotide database. The microbial load and proximate composition of the substrates were determined, and the fermentation media formulated. The organism used for the study was identified as Aspergillus niger MW188538. The results showed a total bacterial count of 9.5x104 cfu/ml, 1.87 x 105 cfu/ml, and 4.0 x 104 cfu/g for cassava whey, banana peels and groundnut shell respectively. The carbohydrates of the cassava whey, banana peels and groundnut shells were 67.74 %w/v, 53.24%w/v and 38.8% w/v respectively. After 12 days of fermentation, the substrates from cassava whey, banana peels, groundnut shells accumulated 2.5 ppm, 1.8 ppm and 1.3 ppm of oxalic acid respectively. The study hypothetically indicates that agro-wastes could be utilized as media components for production of industrial organics.

Challenges and Considerations during In Vitro Production of Porcine Embryos.

Genetically modified pigs have become valuable tools for generating advances in animal agriculture and human medicine. Importantly, in vitro production and manipulation of embryos is an essential step in the process of creating porcine models. As the in vitro environment is still suboptimal, it is imperative to examine the porcine embryo culture system from several angles to identify methods for improvement. Understanding metabolic characteristics of porcine embryos and considering comparisons with other mammalian species is useful for optimizing culture media formulations. Furthermore, stressors arising from the environment and maternal or paternal factors must be taken into consideration to produce healthy embryos in vitro. In this review, we progress stepwise through in vitro oocyte maturation, fertilization, and embryo culture in pigs to assess the status of current culture systems and address points where improvements can be made.

Effects of Biochar and Biochar–Compost Mix on Growth, Performance and Physiological Responses of Potted Alpinia zerumbet

Container crop production has become increasingly popular over the last 50 years. A major component of container or potting media is peat. Peatlands are a natural carbon sink, and peat is a nonrenewable natural resource. Peat harvesting has become an important environmental issue. There is a growing effort to explore alternative organic materials to completely or partially replace peat as a medium component. Biochar is a carbon-rich product that has gained increasing interest as a component of growing media. In the present study, biochar was produced from rice straw. Peat/perlite/biochar (PPB; 40/30/30 v/v) and peat/perlite/biochar/vermicompost (PPBC; 30/30/35/5 v/v) were evaluated relative to a basal or control medium of peat/perlite (PP; 70:30 v/v). Alpinia (Alpinia zerumbet ‘Variegata Dwarf’) was used as a test plant. Amending biochar and biochar–compost mix increased the pH of the growing media. Hydrophysical properties including container capacity, bulk density, air space and total porosity were all within or near the standard ranges for soilless growing media. Chlorophyll a and b contents of A. zerumbet plants grown in PPB medium were reduced by more than 20% and 28%, respectively, compared to those grown in PP or PPBC media. The net photosynthetic rate of PPB-grown plants was more than 28% lower than those grown in PP and PPBC media. As a result, shoot and root dry weights of plants produced in PPB medium were more than 42% and 22% less, respectively, than those grown in PP and PPBC media. Although visual quality of PPB-grown plants was lower, they still exhibited marketable quality, which was largely due to the fact that their side shoots, leaf numbers, leaf areas, leaf thickness, and shoot diameters were comparable to those produced in PP and PPBC media. The present study showed that in a peat/perlite basal medium, substitution of peat by biochar derived from rice straw at 30% affected the growth of A. zerumbet plants, mainly in dry matter accumulation, but the plants were still marketable. On the other hand, plants grown in the same basal medium with peat replaced by the biochar at 35% plus an amendment of compost at 5% were comparable to those grown in the control medium. As the value of ornamental plants depends on their aesthetic appearance, a potting medium comprised of peat/perlite/biochar/vermicompost at 30/30/35/5 by volume is recommended for the production of A. zerumbet plants. The substitution of peat at 35% suggests that peat use can be reduced in the formulation of potting media, thus contributing to the conservation of peatlands.

Photosynthetic apparatus performance and anatomical modulations of Alcantarea imperialis (Bromeliaceae) exposed to selenium during in vitro growth

Elements not usually included in culture medium formulations, such as selenium (Se), may have beneficial effects on micropropagated plants. We evaluated the effects of Se on the physiological and anatomical responses of Alcantarea imperialis during in vitro culture. Plants were cultured in a medium containing a gradient of Se concentrations (0, 4, 8, 16, or 32 µM Se). After 56 d, the growth traits, chlorophyll a fluorescence, and root and leaf anatomy were analyzed. The fresh mass declined at the highest Se concentration. Higher Se concentrations induced bigger stomata, while the stomatal density decreased. Plants cultured with Se had improved PSII and PSI electron transport. This led to higher values of the total performance index. Thus, Se-induced plants showed a higher electron transport dynamics and energy conservation from water to PSI and developed anatomical traits that can favor tolerance to water deficit.

In vitro propagation of six selected sugarcane mutant clones through leaf explants

Six mutant clones of sugarcane with high productivity have been produced through tissue culture techniques combined with mutations using gamma-ray irradiation and Ethyl Methane Sulfonate. The six mutant clones have been tested for stability in the field. They are proven to have high productivity and yields, so that they are very potential to be developed as superior varieties. To support the planting material sufficiency of these clones, an efficient propagation method was needed. Media formulations with different physical properties and composition of growth regulators were tested to obtain high seedling propagation rates. The media formulation for callus induction was Murashige dan Skoog (MS) + 3 mg/l 2,4-D + 3 g/l casein hydrolysate + 3% sucrose and for shoot regeneration was MS + 0,5 mg/l BA + 0,1 mg/l IBA + 100 mg/l PVP and 2% sucrose. Shoot proliferation was carried out on MS liquid (1, ½) + (0.3; 0.5 mg/l) BA + 0.1 mg/l IBA + 1 mg/l Kinetin + (0; 0.5 mg/l) GA3+ sucrose 2%. The results showed that callus induction, callus regeneration, and shoot proliferation of sugarcane mutant clones were influenced by the genotype and medium composition. The fastest callus induction was obtained from the MSP-4 clone (5.82 days), and the longest was MSB-7 (8.82 days). The largest callus diameter was obtained from MSB-6 clone on MS medium containing 1 mg/l BA, 100 mg/l PVP, and 2% sucrose. The highest number of shoots was obtained from the MSB-6 clone, while the least number of shoots conducted from the MSB-8 clone. The MSB-8 clones were more difficult to regenerate compared to the others. The best media formulation for shoot proliferation was ½ MS containing 0.5 mg/l BA, 1 mg/l Kinetin, and 0.1 mg/l IBA, while the best formulation for rooting was ½ MS.

337 DermaCult™, a Serum- and BPE-free Medium Supporting Long-term Feeder-free Expansion of Primary Human Epidermal Keratinocytes that Retain Their Differentiation Potential at Air Liquid Interface

Primary human epidermal keratinocytes (HEKs) typically undergo growth arrest at approximately 15 - 30 population doublings (PDs) when maintained in traditional feeder-free culture medium formulations. To circumvent this early senescence, we developed DermaCult™, a serum- and BPE-free medium that promotes long-term growth (> 30 PDs) of HEKs while maintaining their air liquid interface (ALI) differentiation potential. To measure cell expansion over the entire lifespan of the culture, HEKs derived from either neonatal or adult skin were thawed and seeded onto uncoated tissue cultureware at a density of 1 - 2.5 x 103 cells/cm2, and passaged when cultures reached approximately 50 - 70% confluence. At various time points, keratinocytes were analyzed by qPCR and flow cytometry to detect expression of basal and differentiated cell markers. In separate cultures, keratinocytes were also differentiated by increasing the calcium concentration in feeder- free monolayer culture or establishing ALI culture. Keratinocytes derived from adult or neonatal skin and cultured in DermaCult™ proliferated between 50 - 70 or >70 PDs before undergoing senescence, respectively (n=4). In monolayer cultures, keratinocytes predominantly maintained a cobblestone morphology, expressed basal cell markers (TP63, KRT14, ITGA6), and were devoid of suprabasal marker KRT10 (n=4). Induction of differentiation resulted in the expression of KRT10, LOR, and formation of ZO-1 tight junctions, with concomitant down-regulation of basal markers TP63 and KRT14. In ALI culture, HEKs generated a stratified epithelium with the appropriate cellular architecture and expression of markers similar to their in vivo counterparts. In summary, we have developed an improved and first BPE-free medium for primary HEKs culture that promotes greater expansion of HEKs and maintenance of their differentiation potential at later passages.

Comparable Expressions of Bacillus species on Different Growth Media

Abstract: An investigation was carried out using Pikovskaya Broth (PKB), Luria Bertani Broth (LBB), and Peptone Water (PW) to analyse growth expressions of constructed Bacillus subtilis sub sp and compared to a commercial Bacillus subtilis RIK 1285. The aim was to determine the effect of carbon, nitrogen and other elements at different variations on the metabolic activities under different conditions. The results obtained showed growth density of 4.1 g/ml at 70oC and 3.1 g/ml at pH 6.0; 3.3 g/ml at 70oC and 2.8 g/ml at pH 6.0; 3.8 g/ml at 60oC and 2.6 g/ml at pH 7.0 from PKB, LBB and PW respectively. The growth density of the commercial strain recorded 3.8 g/ml at 50oC and 2.8 g/ml at pH 7.0; 3.1 g/ml at 50oC and 2.3 g/ml; 3.0 at 50oC and 2.3 at pH respectively. The investigation showed importance and relevance of gene metabolic upgrade on the utilization of multiple nutrients present from one media to another. Keywords: media formulation, microbial reaction, growth promoters, growth density

Survival and viability of Bradyrhizobium japonicum in different liquid medium

The microbiological inoculants present on the market come in various formulations and forms. Inoculants used in pre-sowing bacterization of legumes in our country are traditionally prepared on peat as the highest quality carrier of bacteria. However, the requirements of the manufacturer are focused on liquid forms of inoculants. Therefore, the aim of this paper is to determine the optimal composition of the liquid medium that will support the growth of Bradyrhizobium japonicum, the soybean symbiont. Three liquid nutrient medium formulations were included in the study: mannitol-yeast medium, modified mannitol-yeast medium, and glycerol medium stored at two temperatures (4 and 25 °C), and B. japonicum cell viability was determined over 6 months. The results showed that the largest number of rhizobia (on average 4x109 zo 9x108) was obtained on yeast-mannitol medium at 4 °C as well as on modified yeast mannitol medium where their number remained constant throughout the storage time and was 5x107 cfu ml-1 at 25 °C. Further research should include testing other rhizobial protectors in order to increase the number of viable cells in longer time periods.

Optimization and Characterization of Biocellulose Production from Bacteria Isolated from Passion Fruits

Biocellulose is a strong polymer consisting of nanofibrillar structures that produce a large surface area and a microporous structure. This organic polymer is greatly in demand in various industries, such as the paper industry, biomedical industry and cosmetics industry. In this study, biocellulose production from two bacteria known as endophytic bacterium SV845 (M02) and Pantoea ananatis IADCAMBID (M03) isolated from passion fruit was selected. Three different media formulations (Media 1, Media 2, and Media 3) were used in order to optimize the biocellulose production where each media contained a different percentage of carbon sources (glucose and fructose). The highest biocellulose production (1.544 mg/mL) was demonstrated by M02 bacteria strain in Media 1 containing glucose alone which fermented at 30 ºC while at 37 ºC, the highest biocellulose (BC) production was demonstrated by M03 bacteria strain at 2.078 mg/mL in media containing glucose alone (Media 1). Data on pH changes during biocellulose fermentation in all the media were set at an initial pH of 6. The final pH values were observed in the range of 5.34 to 6.08 for M02 strain and 5.95 to 8.53 for M03 strain, respectively. Characterizations of biocellulose were compared to starch using Fourier Transform Infrared (FTIR) spectroscopy. FTIR analysis indicated that the absorption peaks at 3200 cm-1 and 1630 cm-1 were derived from the association of intermolecular and intramolecular hydrogen bonds and H–O–H bending vibration of the absorbed water molecules in cellulose.

Achieving cost-effective, scalable high-titer AAV production

Watch the video or read the poster to learn: Methods to increase AAV titers, reducing overall production costsScalable protocols for suspension systems, from shake flask to bioreactor scaleHow to streamline a helper virus-free triple transfection protocol to simplify workflowChao Yan Liu gained an MD from the Hebei Medical University (Hebei, China) and a Master’s degree in Pathology from the Academy of Medical Sciences in Beijing, China. She got her post-doctoral training at the Blood Research Institute, Medical School of Wisconsin, and then became a research assistant professor at the University of Buffalo, New York. After more than 10 years in academic research in the field of cellular biology and immunology, in 2005 she joined the Research and Development department of the former Life Technologies. She has extensive experience in mammalian cell culture, chemically defined medium formulation, and protein expression. In the past 3 years, she and her team have focused on the AAV-Max viral production system and next-generation protein expression system development.

Improving Fungal Cultivability for Natural Products Discovery.

The pool of fungal secondary metabolites can be extended by activating silent gene clusters of cultured strains or by using sensitive biological assays that detect metabolites missed by analytical methods. Alternatively, or in parallel with the first approach, one can increase the diversity of existing culture collections to improve the access to new natural products. This review focuses on the latter approach of screening previously uncultured fungi for chemodiversity. Both strategies have been practiced since the early days of fungal biodiscovery, yet relatively little has been done to overcome the challenge of cultivability of as-yet-uncultivated fungi. Whereas earlier cultivability studies using media formulations and biological assays to scrutinize fungal growth and associated factors were actively conducted, the application of modern omics methods remains limited to test how to culture the fungal dark matter and recalcitrant groups of described fungi. This review discusses the development of techniques to increase the cultivability of filamentous fungi that include culture media formulations and the utilization of known chemical growth factors, in situ culturing and current synthetic biology approaches that build upon knowledge from sequenced genomes. We list more than 100 growth factors, i.e., molecules, biological or physical factors that have been demonstrated to induce spore germination as well as tens of inducers of mycelial growth. We review culturing conditions that can be successfully manipulated for growth of fungi and visit recent information from omics methods to discuss the metabolic basis of cultivability. Earlier work has demonstrated the power of co-culturing fungi with their host, other microorganisms or their exudates to increase their cultivability. Co-culturing of two or more organisms is also a strategy used today for increasing cultivability. However, fungi possess an increased risk for cross-contaminations between isolates in existing in situ or microfluidics culturing devices. Technological improvements for culturing fungi are discussed in the review. We emphasize that improving the cultivability of fungi remains a relevant strategy in drug discovery and underline the importance of ecological and taxonomic knowledge in culture-dependent drug discovery. Combining traditional and omics techniques such as single cell or metagenome sequencing opens up a new era in the study of growth factors of hundreds of thousands of fungal species with high drug discovery potential.

Fiber enrichment of sourdough bread by inulin rich Jerusalem artichoke powder

This study aims to determine the effects of Jerusalem artichoke powder (JAP) utilization on the physical quality and nutritional characteristics of sourdough bread. JAP was applied to both sourdough fermentation medium and bread formulation. The increasing rates of JAP had deteriorated effects on the physical properties (specific volume, color, and texture profile) of bread. JAP decreased significantly in vitro starch digestion rate in sourdough bread. The glycemic index value of 20% JAP-containing sourdough bread was detected to be 75.12. Additionally, total dietary fiber content increased from 2.62 to 5.30/100 g, by JAP incorporation. The total phenolic content and antioxidant activity of control were determined as 43.72 mg GA/100 g and 48.78 µmol TE/100 g, respectively. In comparison, these values were 80.60 mg GA/100 g and 130.77 µmol TE/100 g in samples supplemented with 20% JAP. According to the sensory analysis, utilization of JAP, over 10% resulted in quite low points. Practical applications Inulin is generally used to increase dietary fiber content and decrease the glycemic index of foods. On the other hand, the utilization of foods with inulin-rich JAP not only increases the dietary fiber content but also enriches it with bioactive components and phytochemicals. However, the high addition levels of JAP can deteriorative effects on the sensory features of foods. In this study, due to the specific aroma and sour taste of sourdough bread, JAP was used above the levels stated in the literature without creating a negative effect on the sensory.