Development of small and medium enterprises (SMEs) is a key approach to achieving economic growth in the Middle East and successful adoption of technology is vital for SMEs' success and continuity. Artificial intelligence (AI) is part of a new generation of technologies that can facilitate competitive advantage but currently there is a lack of evidence regarding AI applications in relation to B2B SMEs in Middle East countries. Therefore, this study empirically examines antecedents to, and consequences of, successful acceptance of AI practices by B2B SMEs in Saudi Arabia. A conceptual model based on the technology-organisation-environment framework is developed which considers the impact of AI enablers and AI readiness on the acceptance of AI practices, and the impact of this on relational governance, performance, and SMEs' AI-based business customer interaction. The conceptual model was tested using structural equation modelling of survey data collected from B2B SMEs (n = 392). The results showed that, of the AI enablers, acceptance of AI practices was significantly influenced by both technology roadmapping and attitude but not professional expertise. Of the AI readiness variables, acceptance of AI practices was significantly influenced by infrastructure and awareness but not technicality. The acceptance of AI practices was found to significantly affect AI-enabled relational governance and performance, and SME's business customer AI-based interaction. This study provides a broader base for theoretical and practical understanding of issues related to AI practices in SMEs and the B2B sector in general.