Mediterranean Region Research Articles

Production of a new chitinase from Nocardiopsis halophila TN-X8 utilizing bio-waste from the blue swimming crab: enzyme characterization and immobilization.

In accordance with the framework of the Circular Blue Bioeconomy in the Mediterranean region, the objective of this study was to evaluate the biotransformation of blue swimming crab (Portunus segnis) residues obtained from the port of Sfax by an extracellular chitinase produced by Nocardiopsis halophila strain TN-X8 isolated from Chott El Jerid (Tozeur, Tunisia). From the analysis of multiple extremophilic Actinomycetota, it was determined that strain TN-X8 exclusively utilized 60g/L of raw blue swimming crab as its carbon and energy source, achieving a chitinase activity of approximately 950 U/mL following a 6-day incubation period at 40°C. Pure chitinase, designated as ChiA-Nh30, was obtained after heat treatment, followed by ammonium sulfate fractionation and Sephacryl® S-200 column chromatography. The maximum ChiA-Nh30 activity was observed at pH 3 and 75°C. Interestingly, compared with cyclohexamidine, ChiA-Nh30 showed a good antifungal effect against four pathogenic fungi. Furthermore, when using colloidal chitin as substrate, ChiA-Nh30 demonstrated a higher degree of catalytic efficiency than the commercially available Chitodextrinase®. In addition, ChiA-Nh30 could be immobilized by applying encapsulation and encapsulation-adsorption techniques. The kaolin and charcoal used acted as excellent binders, resulting in improved ChiA-Nh30 stability. For the immobilized ChiA-Nh30, the yield of N-acetyl-D-glucosamine monomers released from 20% (w/v) blue swimming crab residues increased by 3.1 (kaolin) and 2.65 (charcoal) times, respectively.

Design storm parameterisation for urban drainage studies derived from regional rainfall datasets: A case study in the Spanish Mediterranean region

ABSTRACT A significant amount of information on regional rainfall characteristics is available nowadays, allowing its use in hydrological applications. This article is motivated by the availability of regional studies regarding maximum daily rainfall and intensity–duration–frequency curves that can be coupled with the design storm concept for urban hydrology studies. This is accomplished through a convenient index describing temporal variability of rainfall. More precisely, a methodology for regionalising the two parameters (i0, φ) of the two-parameter gamma design storm (G2P) is developed herein. A three-step methodology is proposed for obtaining the two parameters (i0, φ) for a given location. The results obtained in a case study show coherence with previous studies concerning maximum rainfall statistics.

Current status of pistachio diseases in countries of the Mediterranean Basin

AbstractIn the Mediterranean Basin, the pistachio crop is considered an emerging nut crop due to the dependence of the European nut markets on international imports. Consequently, the extension and intensification of pistachio production to nontraditional growing areas has promoted both classic and emerging diseases of this nut tree, which are limiting factors in crop production. Due to the limited interest in pistachio crops, the aetiology and epidemiology of the main pistachio diseases are poorly studied. Thus, this review summarizes the state of pistachio disease research, with particular attention given to the Mediterranean area. The information reported here is based not only on the literature, but also the advancement of our research on pistachio diseases conducted in both Italy and Spain. We describe the main fungal diseases of the tree canopy in Mediterranean countries, such as Botryosphaeria panicle and shoot blight, Septoria leaf spot, and stem, branch and trunk canker diseases, followed by the main soilborne diseases. In addition, we review minor and/or sporadic fungal diseases originating in not only the Mediterranean, but also other pistachio‐producing countries. Another section is dedicated to other diseases caused by bacteria, phytoplasmas, viruses and nematodes. Finally, we summarize the main control strategies adopted in Mediterranean Basin countries against pistachio diseases.

Environmental Factors Related to Climate Change Alter the Chemical Composition and Biological Activity of Lavandula viridis L’Hér Essential Oil

Climate change is affecting all regions of the world, and the Mediterranean region is one of the most affected. Plants accumulate secondary metabolites as an adaptive response to stress circumstances. The present study investigated the effect of different abiotic factor conditions (drought, moderate heat, severe heat, salinity, and UV-B radiation) on the essential oil (EO) yield, composition (volatile profile), and biological activity (enzyme inhibition and antioxidant activity) of Lavandula viridis L’Hér. In general, the environmental conditions increased the extraction yield of EO. Eighty-two compounds were identified in the EO and environmental factors induced some quantitative changes in EO composition. Severe heat and salinity conditions increased the concentration of the two most abundant compounds, 1,8-cineole and camphor. Severe heat also increased the potential of EO to inhibit the enzymes butyrylcholinesterase and tyrosinase. Drought, salinity, and UV-B radiation promoted the ability of EO to inhibit acetylcholinesterase. In addition, heat and drought enhanced the antioxidant activity of EO. These results are relevant for exploring the potential of this EO for industrial applications, although future studies combining the factors studied are important to understand the influence of synergistic effects on the composition and bioactivity of the plant products obtained.

A methodological approach to rock art survey and recording via drone. The application to the Rock Art of the Mediterranean Basin of the Iberian Peninsula assemblage

The significant advancement in drone technology has led to increased usage across different scientific domains. In the field of archaeology, drones became increasingly popular a decade ago, primarily for photogrammetric documentation or aerial photography. Since then, researchers have experimented with new applications, notably utilizing LiDAR imagery to enhance archaeological surveying. In this context, one of the latest applications involves surveying open-air rock art shelters in inaccessible locations to search for prehistoric rock art imagery. The current study involves refining the methodology used for this purpose in the territory of UNESCO’s World Heritage List property Rock Art of the Mediterranean Basin of the Iberian Peninsula, utilizing the DJI Mavic 3 foldable drone, which represents a significant improvement over previous models. On the other hand, it highlights the potential for its utilization in conservation studies and managing human activity in their environments, considering the threats to which these sites are currently exposed.

Quantitative risk assessment for the introduction of bluetongue virus into mainland Europe by long-distance wind dispersal of Culicoides spp.: A case study from Sardinia.

Europe faces regular introductions and reintroductions of bluetongue virus (BTV) serotypes, most recently exemplified by the incursion of serotype 3 in the Netherlands. Although the long-distance wind dispersal of the disease vector, Culicoides spp., is recognized as a virus introduction pathway, it remains understudied in risk assessments. A Quantitative Risk Assessment framework was developed to estimate the risk of BTV-3 incursion into mainland Europe from Sardinia, where the virus has been present since 2018. We used an atmospheric transport model (HYbrid Single-Particle Lagrangian Integrated Trajectory) to infer the probability of airborne dispersion of the insect vector. Epidemiological disease parameters quantified the virus prevalence in vector population in Sardinia and its potential first transmission after introduction in a new area. When assuming a 24h maximal flight duration, the risk of BTV introduction from Sardinia is limited to the Mediterranean Basin, mainly affecting the southwestern area of the Italian Peninsula, Sicily, Malta, and Corsica. The risk extends to the northern and central parts of Italy, Balearic archipelago, and mainland France and Spain, mostly when maximal flight duration is longer than 24h. Additional knowledge on vector flight conditions and Obsoletus complex-specific parameters could improve the robustness of the model. Providing both spatial and temporal insights into BTV introduction risks, our framework is a key tool to guide global surveillance and preparedness against epizootics.

Antimycobacterial and Anticancer Properties of Myrtus communis Leaf Extract

Background: Plant-derived products or extracts are widely used in folk/traditional medicine to treat several infections, ailments, or disorders. A well-known medicinal herb, Myrtus communis is an evergreen fragrant plant native to the Mediterranean region that has been used for ages in traditional medicine around the world. Materials and methods: The microplate alamarBlue assay and the well diffusion method were used to evaluate the zone of inhibition and MIC, respectively. The double-disc diffusion method was used to investigate the synergy between antibiotics and the extract. The crystal violet method was used to investigate biofilm development. The SulphoRhodamine-B assay and DNA flow cytometry were used to investigate the proliferation and subsequent distribution of cells among different phases of the cell cycle. The apoptotic and necrotic phases of the cancer cells were examined using flow cytometry in conjunction with Annexin V-FITC/PI labeling. Using the IBM SPSS statistical program, a one-way ANOVA with Tukey’s post hoc test was employed for statistical analysis. Results: The ethanolic leaf extract of M. communis showed a strong growth inhibition effect (zone of inhibition: 20.3 ± 1.1–26.3 ± 2.5 mm, MIC: 4.88–312.5 µg/mL, and MBC: 39.07–1250 μg/mL) against several rapidly growing and slow-growing mycobacterial strains in a dose-dependent manner. Damage to the cell wall of bacterial cells was determined to be the cause of the antimycobacterial action. The extract inhibited biofilm formation (MBIC of 9.7 µg/mL) and eradicated already-formed mature and ultra-mature biofilms of M. smegmatis, with MBEC values of 78 µg/mL and 156 µg/mL, respectively. Additionally, the extract exhibited potent anticancer effects against diverse cancer cell lines of the breast (MCF-7), liver (HepG2), cervix (HeLa), and colon (HCT116) (IC50 for HCT116: 83 ± 2.5, HepG2: 53.3 ± 0.6, MCF-7: 41.5 ± 0.6, and HeLa: 33.3 ± 3.6) by apoptosis after arresting the cells in the G1 phase of the cell cycle. Conclusions: These results suggest that M. communis leaf extract is a potential source of secondary metabolites that could be further developed as potential anticancer and antimycobacterial agents to treat diverse types of cancers and mycobacterial infections.

Impact of heat waves on human morbidity and hospital admissions in a city of the western mediterranean area.

The effect of heat waves on mortality is well known, but current evidence on morbidity is limited. Establishing the consequences of these events in terms of morbidity is important to ensure communities and health systems can adapt to them. We thus collected data on total daily emergency hospital admissions, admissions to critical care units, emergency department admissions, and emergency admissions for specific diagnoses to Hospital Universitario de Son Espases from 1 January 2005 to 31 December 2021. A heat wave was defined as a period of ≥ 2days with a maximum temperature ≥ 35°C, including a 7day lag effect (inclusive). We used a quasi-Poisson generalized linear model to estimate relative risks (RRs; 95%CI) for heat wave-related hospital admissions. Results showed statistically significant increases in total emergency admissions (RR 1.06; 95%CI 1 - 1.12), emergency department admissions (RR 1.12; 95%CI 1.07 - 1.18), and admissions for ischemic stroke (RR 1.26; 95%CI 1.02 - 1.54), acute kidney injury (RR 1.67; 95%CI 1.16 - 2.35), and heat stroke (RR 18.73, 95%CI 6.48 - 45.83) during heat waves. Heat waves increase hospitalization risk, primarily for thromboembolic and renal diseases and heat strokes.

Comprehensive Risk Assessment for Identifying Suitable Residential Zones in Manavgat, Mediterranean Region

The absence of comprehensive risk analysis in residential development within certain regions often leads to substantial human and material losses during natural disasters. The Mediterranean region, particularly susceptible to the impacts of climate change, is projected to witness an upsurge in the frequency of natural calamities like floods, landslides, and forest fires. Consequently, meticulous risk assessment during the selection of residential areas becomes paramount in this context. This study is dedicated to the evaluation of suitable residential zones in Manavgat, a pivotal location in the Mediterranean region with a progressively growing population. The findings indicate that approximately 4.26% of the research area is deemed appropriate for residential establishment. The identification of these locations is crucial for ensuring human and material safety, as well as enhancing overall biocomfort. Moreover, this study provides a foundation for long-term planning initiatives within the region and makes a significant contribution to the international evaluation literature by demonstrating the application of integrated risk assessment methodologies in urban planning.

Exploring scale insects biodiversity in orange orchards: insights from Guelma province, Algeria

Scale insects are pests affecting citrus crops in the Mediterranean basin, but their significance is often overlooked in some countries due to insufficient information about the threat they pose to this type of cultivation. In Algeria, various studies have aimed to identify and highlight the most harmful species impacting citrus orchards. This research specifically focuses on compiling a list of species affecting orange orchards in three regions of Guelma province (northeast Algeria) which are Boumahra Ahmad, El Fedjoudj and Guelma. Branch samples with their leaves were collected from all cardinal directions of the trees as well as from the center of the canopy during the winter-spring period from 2014 to 2018 while examining the climatic influence on their presence. Seventeen species were identified, with Aonidiella aurantii (Maskell, 1879), Parlatoria ziziphi (Lucas, 1853) and Coccus pseudomagnoliarum (Kuwana, 1914) being dominant and Lepidosaphes beckii (Newman, 1869), Saissetia oleae (Olivier, 1791) and Saissetia coffeae (Walker, 1852) considered rare. Non-parametric richness estimators (Chao 1, Chao 2, Jack 1 and Boot) suggest the potential presence of additional species, reaching 20.15 ± 0.96, indicating that the count may extend to 21 scale insects in the surveyed orchards. Spearman correlation analysis applied to species and climatic conditions reveals their influence on species abundance, but not on their diversity. We observed a positive impact on relative humidity and a negative impact on average temperature.

Revegetation is key for soil organic carbon sequestration on abandoned and degraded land in northern Spain

Agricultural land abandonment is a major land use change in the Mediterranean region, especially affecting marginal areas. The fields of the abandoned village Sierra Estronad (Aragón, Spain), experienced heavy impact treatments (bulldozing) after which half of the fields were kept open and tilled without planting any crop and the other half of the fields were left fallow. From these two treatments and the surrounding natural forest 483 soil samples were collected in addition to corresponding vegetation data at 162 GPS referenced sampling points. Soil samples were analyzed using predictive models based on visible and near-infrared spectroscopy for Soil Organic Carbon (SOC), total Nitrogen, and Permanganate Oxidizable Carbon.Comparing the fallow fields, which have had a 15-year recovery period to the tilled fields, a SOC sequestration rate of 0.64 Mg ha−1 y−1 was found. On tilled fields however, even after a recovery period of 5 years, very few plants were able to colonize the area, resulting in a sparse soil cover and significantly lower SOC and total N stocks.These results show the interdependence of soil fertility proxies (SOC and /total Nitrogen) and the degree of vegetation cover, and how practices of preventing former agricultural fields from revegetating have a long-lasting impact of soil degradation, even after their termination. However, if left fallow, abandoned fields do have the potential to support a secondary succession and serve as a carbon sink thus contributing to soil fertility and climate change mitigation.

Maritime Linkages between the Han Dynasty and Parthian Empire: Evidence from Unearthed Persian Pottery

This study describes a Persian pottery jug unearthed from Hepu, a well-known port for foreign trade in China during the Han Dynasty. The jug is the earliest and only Persian object dated to the Han Dynasty discovered in China. It thus serves as one of the most significant physical pieces of evidence for the investigation of trade between the Han Dynasty and Southeast Asia, South Asia, and even West Asia. This article takes the Persian jug as its starting point, and combines archaeological data from the same period unearthed from other port sites in Southeast Asia and South Asia, as well as Chinese and foreign historical documents, to recover the sea route via which the Persian jug moved from West Asia through South and Southeast Asia to Hepu, to confirm that the Han Dynasty had established indirect or direct maritime links with the Parthian Empire by the second century A.D. at the latest. This conclusion is supported with the discovery of glass and bronze cymbals from Western Asian and Mediterranean regions in Hepu and Panyu, the latter of which served as the largest foreign goods distribution center in the southern coastal area of China.

Maritime Linkages between the Han Dynasty and Parthian Empire: Evidence from Unearthed Persian Pottery

This study describes a Persian pottery jug unearthed from Hepu, a well-known port for foreign trade in China during the Han Dynasty. The jug is the earliest and only Persian object dated to the Han Dynasty discovered in China. It thus serves as one of the most significant physical pieces of evidence for the investigation of trade between the Han Dynasty and Southeast Asia, South Asia, and even West Asia. This article takes the Persian jug as its starting point, and combines archaeological data from the same period unearthed from other port sites in Southeast Asia and South Asia, as well as Chinese and foreign historical documents, to recover the sea route via which the Persian jug moved from West Asia through South and Southeast Asia to Hepu, to confirm that the Han Dynasty had established indirect or direct maritime links with the Parthian Empire by the second century A.D. at the latest. This conclusion is supported with the discovery of glass and bronze cymbals from Western Asian and Mediterranean regions in Hepu and Panyu, the latter of which served as the largest foreign goods distribution center in the southern coastal area of China.

Zika and Chikungunya in Europe 2100 – A GIS based model for risk estimation

BackgroundThe spread of vector-borne infectious diseases is determined, among other things, by temperature. Thus, climate change will have an influence on their global distribution. In the future, Europe will approach the temperature optimum for the transmission of ZIKV and CHIKV. Climate scenarios and climate models can be used to depict future climatic changes and to draw conclusions about future risk areas for vector-borne infectious diseases. MethodsBased on the RCP 4.5 and RCP 8.5 climate scenarios, a geospatial analysis was carried out for the future temperature suitability of ZIKV and CHIKV in Europe. The results were presented in maps and the percentage of the affected areas calculated. ResultsDue to rising temperatures, the risk areas for transmission of ZIKV and CHIKV spread in both RCP scenarios. For CHIKV transmission, Spain, Portugal, the Mediterranean coast and areas near the Black Sea are mainly affected. Due to high temperatures, large areas throughout Europe are at risk for ZIKV and CHIKV transmission. ConclusionTemperature is only one of many factors influencing the spread of vector-borne infectious diseases. Nevertheless, the representation of risk areas on the basis of climate scenarios allows an assessment of future risk development. Monitoring and adaptation strategies are indispensable for coping with and containing possible future autochthonous transmissions and epidemics in Europe.

Prevalence, Severity, and Risk Factors of Eczema Among Young Children and Adolescents in Saudi Arabia: A National Cross-sectional Study, 2019

BackgroundEczema is a common inflammatory skin disease with a significant global health burden. Eczema has a significant impact on quality of life. ObjectiveWe aimed to estimate the prevalence, severity, and risk factors associated with eczema among schoolchildren in Saudi Arabia. MethodsUsing the standardized Global Asthma Network questionnaires and methodology, a nationwide cross-sectional study was carried out across 20 regions in Saudi Arabia between March and April 2019. Data were collected from 137 primary schools and 140 intermediate schools using a multistage stratified cluster sampling method. ResultsThe study included 3614 young children aged 6-7 years and 4068 adolescents aged 13-14 years. Current eczema was prevalent among 4.5% of children and 5.1% of adolescents. Severe eczema was reported in 0.8% and 0.9% of young children and adolescents, respectively. Several factors showed significant association with eczema. Among children, eczema was linked positively to having a history of chest infections and wheezing in early life as well as ever attending day care and current exposure to cats. Among adolescents, the main potential risk factors included paracetamol use in the previous year, adherence to a lifestyle of vigorous physical activity, and current exposure to cats. Conversely, high consumption of nuts was found to be negatively associated with eczema. ConclusionThe prevalence of eczema in schoolchildren in Saudi Arabia is lower than the global average but within the average range for the Eastern Mediterranean region. Further studies should be conducted in Saudi Arabia to identify variation among different regions.

Proposal for a Managed Aquifer Recharge feasibility index for southern Portugal using multi-criteria decision analysis

Water scarcity in the Mediterranean region requires the adoption of new and optimized water resources management tools that allow a progressive climate change adaptation, being one of the main drivers for the employment of alternative water storage measures such as Managed Aquifer Recharge (MAR). To promote the implementation of these measures, this study evaluates the application of a MAR feasibility index mapping to southern Portugal, employing Multi-Criteria Decision Analysis (MCDA). A participatory approach – developed within the AGREEMAR project – aims for the characterization of the intrinsic site suitability, water availability and demand, where the stakeholder inputs are embedded in the weighting computation process.The results obtained demonstrate prevalent moderate MAR feasibility across the entire region (78% of the area) with an increased percentage of the area corresponding to high MAR feasibility (22%) strongly related to highly suitable geological features. A comparative analysis with the Analytical Hierarchical Process (AHP) showed minor high MAR feasibility areas (7%), and feasibility class transitions between the directly above or below classes.A sensitivity analysis, conducted based on a one-at-a-time (OAT) variation of the criteria weights, assessed the impact of the weighting methods on feasibility class changes. For the AGREEMAR method, changes in the weight of 10 of the 28 considered criteria resulted in negligible changes when compared to the original feasibility map, while for AHP 3 out of 28 produced minor changes. The remaining criteria showed important modifications in the feasibility maps, which underlined AGREEMAR method weights provided higher stability in the results.The study provides information about a MAR site selection process procedures, allowing replication. The dissemination of the results is expected to raise awareness of MAR among stakeholders and support scientifically-based water resources management decision-making.

First report of the nematode‐trapping fungus Arthrobotrys thaumasia in Türkiye and its biocontrol potential against Meloidogyne incognita

AbstractRoot‐knot nematodes, particularly, Meloidogyne incognita, are among the most destructive endoparasitic nematodes, infecting a diverse range of plant hosts. Nematode‐trapping fungi are known for their potential application as biological control agents against plant parasitic nematodes. In the present study, the nematode‐trapping fungus Arthrobotrys thaumasia was isolated from the rhizosphere soil of tomato plants in the West Mediterranean Region of Türkiye. Two hundred and twenty‐three tomato plant rhizosphere soil samples yielded six nematode‐trapping fungal isolates, giving an occurrence frequency of 2.69%. Using morphology and molecular marker sequences (ITS and β‐tubulin loci), the species of the fungi was confirmed to be A. thaumasia. In vitro, A. thaumasia reduced second‐stage juveniles of M. incognita by 77.5% (isolate I‐Y4‐2) and 72.5% (isolate B‐G5‐1). This is the first report on the isolation and characterization of the nematode‐trapping fungus A. thaumasia from Türkiye. Two isolates of A. thaumasia (I‐Y4‐2 and B‐G5‐1) appear to be promising biological control agents that may be utilized for controlling M. incognita‐caused root‐knot diseases.

Drug-use disorders in the Eastern Mediterranean Region: a glance at GBD 2019 findings.

The aim of this study is to investigate drug use disorders which are a major cause of Disability Adjusted Life Years (DALYs) in the Eastern Mediterranean Region (EMR). This article is a part of the global burden of diseases (GBD), injuries, and risk factors 2019 study. The GBD modeling approach was used to estimate population-level prevalence of drug use disorders. We combined these estimates with disability weights to calculate years of life lived with disability (YLDs), years of life lost (YLLs), and disability-adjusted life-years (DALYs) for 1990-2019. It is estimated that in 2019 in EMR around 3.4 million people have drug use disorder which has increased by 137% compared to 1990. Also, in 2019, DALY number for drug use disorders was 1217.9 (95% UI: 940.4, 1528.9) thousand years and 7645 (95% UI: 6793.7, 8567.9) deaths occurred. The DALY rate increased 39.6% in the region since1990, whereas the global rate increased by 24.4%. United Arab Emirates, Libya, and Iran were most affected by drug use disorders with the highest rates of age-standardized DALY in EMR in 2019. The most prevalent drug use disorder in the region is opioid use which is accountable for 80% of all drug use disorders DALYs. Despite many interventions, drug use disorders are still responsible for high rates of DALY in the region which has increased since 1990 in both males and females; more comprehensive policies, better control measures and proper education could reduce the adverse effects.

Updating the fauna and age of the Neogene-Quaternary large mammal sites of Greece

The Neogene-Quaternary continental deposits cover large areas of Greece and several fossil mammal sites have been discovered in the country. There are several collections of large fossil mammals for this time span, which provide important data for the fauna biochronology, correlations, palaeoecology and palaeogeography of the area. The last lists with the fauna, age and biochronology of the Greek large mammal localities were given in 2006 for the Neogene and in 2001 for the Quaternary. Extensive research over the last twenty years provided several new localities, and many data and information were published. Therefore, an updating of the lists was more than necessary, and the present article deals with it; it covers the time span untill June 2023. Lists include all faunal information as the systematic classification, chronology, biostratigraphic correlation of the faunas, and main bibliography. It is worth mentioning that some of the Greek localities are key-localities for the Eastern Mediterranean region, e.g., Pikermi, Axios Valley, Villafranchian collection. These faunas are important for the comparison and identification of new materials, correlations, palaeoecology, and palaeogeography of Neogene European mammals.

Old stories, new cases. Viral infectious diseases and children's health

During the 20th century, smallpox has produced between 300–and 500 million victims. In 1796, Edward Jenner conducted one of the first modern immunizations when he inoculated an 8-year-old boy using material from a cowpox lesion. In 1959, WHO (World Health Organization) launched the Smallpox Eradication Programme. By 1980, WHO declared smallpox officially eradicated. Due to genetic and antigenic similarities between the monkeypox and smallpox viruses, the cessation of smallpox vaccination since 1980′s contributed to the wanning of cross‐protection against monkeypox (mpox). Outbreaks of human mpox has spread across 110 countries.In 1916 and 1952, two major poliovirus outbreaks took place in the USA. The virus killed or paralyzed over half a million people yearly. Introduction of the inactivated polio vaccine (IPV,1955) and oral polio virus (OPV,1963) led to decline in the number of cases by 99.99 %. The Global Polio Eradication Initiative (GPEI) was launched in 1988 and five of six WHO regions have been certified free of wild polio virus (WPV), except Eastern Mediterranean region. Africa had been declared WPV free, unfortunately cases of paralysis due to wild virus in Malawi and Mozambique were detected having been imported from Pakistan. Outbreaks of circulating Vaccine Derived Polio Virus type 2 (cVDPV2) have expanded in the Eastern Mediterranean and African Region from October 2022 to February 2023. Measles, being one of the most contagious infections, requires high population immunity (>95 %) to impede transmission. In 1963, a measles vaccine became available. Finland was the first country to eradicate measles by using the 2 dose-measles vaccines. The region of the Americas was verified to have eliminated measles in 2000. The COVID-19 pandemic disrupted global vaccination activities. Vaccination coverage has fallen from 90 % to 50 % in western countries. Over 30 000 measles cases were reported from 40 WHO European Region members in 2023.