Medinilla Speciosa Research Articles

Carbon dots nanoparticles derived from showy Asian grapes (Medinilla speciosa) for free radical scavenger

Carbon dot nanoparticles (CNPs) were synthesized from the peels of showy Asian grapes (Medinilla speciosa) using microwave irradiation for free radical scavenging. The optimal emission and absorption intensities were achieved with a 50-min irradiation duration, at wavelengths of 430 nm and 267 nm, respectively. XRD analysis revealed characteristic peaks at angles of 26.37° and 29.63°. FTIR analysis indicated that antioxidant potential is attributed to hydroxyl and carboxyl groups on the CNPs’ surface. TEM images showed diameters ranging from 2 nm to 8 nm. The CNPs exhibited 73.82% antioxidant activity at 5 mg/mL, with an IC50 value of 6.89 ppm, indicating strong antioxidant activity. Additionally, the CNPs demonstrated good storage stability, suggesting potential applications in food supplements and biomedical materials.

Fortification of local fruit parijoto on soy yogurt drink and economy analysis

Background: The food and beverage industry is a leading industry that is the government's focus in the Industrial Revolution 4.0. Culinary is the most popular economic sub-sector.. As a food product from biotechnology, yoghurt always manages to occupy a global market share. Yoghurt is proven to maintain the digestive system. Therefore innovation is needed, especially regarding materials, to boost product competitiveness. Animal milk substrate can be substituted with soy milk which is cheap but rich in protein. The addition of natural fortification is projected to improve product quality. Parijoto fruit (Medinilla speciosa) typical of the slopes of Mount Muria, Colo Village, Kab. Kudus was chosen as a fortificant because it is rich in antioxidants and antibacterial pathogens. At the same time, this adds to the usability and economic value of Parijoto, which is still low so far. Method: The research was conducted experimentally, referring to previous research methods. The formulation design was varied regarding adding Parijoto fruit extract to 100 ml of the product. Findings: From the results of the organoleptic test, the F1 formula (addition of 5 ml of Parijoto fruit extract) received the best acceptance from the panellists, with a preference level of 4.89. The product characteristics are liquid texture, alcoholic sweet taste, cream colour, and the aroma of Parijoto fruit tends to be faint. Conclusion:Parijoto Yogurt has advantages in terms of locality, price and nutrition. However, further research is needed based more collaboration to increase this product's health and economic impact local society. Novelty/Originality of this article: This study creates new functional food products and opens up opportunities to revitalize local economies and conserve biodiversity.

Formulation of nanoemulsion parijoto fruit extract (Medinilla Speciosa) with variation of tweens stabilizers.

Nanotechnology has substantial potential for development due to its ability to modify surface characteristics and particle size, facilitating enhanced absorption of functional food compounds and controlled release of active substances to mitigate adverse effects. Nanoemulsion, a stable colloidal system formed by blending oil, emulsifier, and water, was identified as nanotechnology with promising applications. However, investigations into the impact of surfactants on characteristic nanoemulsions need to be more varied. This research gap necessitated further exploration in the advancement of nanotechnology-based foods. The parijoto fruit (Medinilla speciosa), an indigenous plant species in Indonesia, has yet to undergo extensive scrutiny for its potential use as a functional and nutraceutical food. Anthocyanins, a principal compound in the parijoto fruit, had exhibited efficacy in reducing the risk of cardiovascular disease diabetes, demonstrating anti-inflammatory and antioxidant properties. This study aimed to investigate the characteristics of nanoemulsion formulations derived from parijoto fruit extract and to evaluate an optimum condition with various tween surfactants. The findings from this investigation could furnish valuable insights for the further advancement of anthocyanin nanoemulsions from parijoto fruit extract. The results comprised the characterization of nanoemulsion particle size, polydispersity index, ζ-potential, conductivity, pH, and viscosity. Through mathematical modeling and statistical methods, RSM optimizes nanoemulsion by examining the relationships and interactions between independent and response variables. Furthermore, the characterization of nanoemulsion encompassed ζ-potential, polydispersity, particle size, conductivity, pH, and viscosity. Elevated surfactant concentrations resulted in diminished particle sizes and more uniform size distribution, albeit reaching a plateau where surfactant aggregation and micelle formation ensued. Increased concentrations of surfactant type, concentration, and parijoto extract impacted the physical characteristics of nanoparticle size and polydispersity. The optimal process conditions for nanoemulsion consisting of the type of Tween used are Tween 80, Tween concentration of 12%, and parijoto fruit extract concentration of 7.5%, yielding a desirability value of 0.74, categorizing it as moderate.

The effect of shade and drought on the growth and proline content of Medinilla speciosa and Medinilla verrucosa plants

Abstract Medinilla is a cultivated wild plant because it has medicinal properties. This research aims to determine the level of resistance and growth of Medinilla speciosa and Medinilla verrucosa plants in drought and shade conditions. The research was carried out in April 2022 – April 2023 in Pakem District Sleman Regency. Analysis of proline and chlorophyll content was carried out at the Gadjah Mada University Laboratory. This study used a nested complete factorial randomized block design, with the types of Medinilla speciosa, Medinilla verrucosa and the volume of water given, 1750 ml, 1500 ml, 1250 ml per polybag nested in 25%, 50% and 75% shade. Each treatment was repeated three times, resulting in a total sample of 54 plants. The results of the study showed that the volume of water provided in the shade suppressed growth, that is number of leaves, leaf area, root volume, fresh weight of stover, chlorophyll except plant height, dry weight of stover and proline content. Water is one of the essential factors needed by plants to grow and develop well. Appropriate light intensity impacts plant growth and production. Light intensity stress and water supply volume stress are part of abiotic stress that can affect growth morphology productivity.

The Potential of Parijoto Fruit Extract Fraction (Medinilla speciosa Blume) as Antibiofilm against Staphylococcus aureus Bacteria

Parijoto fruit extract (Medinilla speciosa Blume) contains flavonoids, saponins, tannins, and terpenoids and has antibacterial potential in inhibiting the growth of Staphylococcus aureus bacteria. S. aureus bacteria are bacteria that are capable of forming biofilms. This research aims to determine the potential of extracts and fractions (water, ethyl acetate, and n-hexane) in inhibiting the formation and degradation of S. aureus biofilms. Parijoto fruit was extracted using the maceration method with 96% ethanol solvent. The extract was fractionated using a liquid-liquid partition technique with water, ethyl acetate, and n-hexane as solvents. The antibiofilm activity test against the S. aureus bacteria ATCC 25923 was carried out by treatment inhibiting biofilm formation and biofilm degradation. The method is to read absorbance using a microplate reader with a wavelength of 595 nm. Optical density results to calculate IC50 and EC50 values. The results showed that the extract and fractions (water, ethyl acetate, and n-hexane) had the potential to inhibit the formation and degradation of S. aureus bacterial biofilms. The IC50 of biofilm inhibition in the extract, water fraction, ethyl acetate, and n-hexane against S. aureus bacteria was 2.51; 1.03; 1.10; 1.52 mg/ml. The EC50 value of the biofilm degradation test on the extract, water fraction, ethyl acetate, and n-hexane against S. aureus bacteria was 1.03; 1.17; 1.37; 1.88 mg/ml. The most active fraction of parijoto fruit (Medinilla specosa Blume) in inhibiting and degrading S. aureus bacterial biofilms is the water fraction.

An activity of flavonoid derived compounds from Medinilla speciosa as anti-hyperpigmentation against tyrosinase proteins with in silico methods

Parijoto (Medinilla speciosa), a typical plant from Indonesia, contains flavonoid compounds as antioxidants. It is able to depigment skin by inhibiting tyrosinase activity during melanogenesis, or melanin formation. Melanogenesis occurs through UV light exposure; it stimulates ROS production and triggers the formation of free radicals and melanogenesis. If not prevented, it will have negative impacts on health. Currently, there is no further research regarding the existence of flavonoid compound derivatives found in parijoto fruit. The aim of this research is to initially identify potential flavonoid derivative compounds as tyrosinase inhibitors using in silico methods. The results can be used as an initial reference for making products based on natural ingredients with minimal side effects. This research uses a bioinformatics approach with the molecular docking method of ligands towards proteins. The stages in this research include: downloading and preparing receptors and ligands; docking with Autodock Vina; visualization of results with Biovia Discovery Studio; data analysis; and discussion. The analysis is carried out by looking at the affinity energy values and complex conformation between the receptor-ligand. The results show flavonoid derivative compounds have the potential to act as tyrosinase inhibitors, as proven by compounds interactions with the active site of tyrosinase to produce the amino acid residues phenylalanine, proline, asparagine, arginine, and histamine. Produce respective affinity energy values, namely -8.9; -7.7; -7.6; -7.5; -7.5; and 7.4 kcal/mol for chalcone, catechin, flavonol, flavanol, flavone, and flavonone compounds. Meanwhile, the comparison compound used is kojic acid, with an affinity energy of -5.5 kcal/mol.

Health benefits of aqueous and ethanolic extracts of medinilla speciosa blume

Medinilla speciosa (local name: parijoto) has many pharmacological benefits, including for treating female infertility. This study aimed to identify bioactive compounds of the aqueous and ethanolic extracts of Medinilla speciosa; to find information on their pharmacological benefits, and to do its docking profile with the protein phosphatase1, which is associated with the enhancement of female fertility. Articles were searched from PubMed. The components of Medinilla speciosa were analyzed with LC-MS/MS. In silico study was conducted based PubChem, Protein Data Base, and Swiss ADME. Pyrx 0.8 and Discovery Studio Visualizer v21.1 were used to predict the interaction. Four flavonoids were identified, namely fisetin, robinetin, luteolin, and kaempferol. Except for robinetin, they exist in glycosidic form. One polyphenol, ellagic acid, was also identified. Literature studies showed they have various pharmacological benefits, such as antioxidants, anti-inflammation, anticancer, antidiabetes, organ protection, and antimicrobial. However, no information is available on its potential for fertility enhancement. Docking analysis showed that the bioactive compounds interact with the A and C chains of the catalytic domain of protein phosphatase 1 (PP1). Aqueous and ethanolic extracts of Medinilla speciosa possess fisetin, robinetin, luteolin, kaempferol, and ellagic acid that bind to catalytic chains of protein phosphatase 1.

Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Etanol Buah Parijoto (Medinilla speciosa Blume) dengan Metode Spektrofotometri UV-Vis

Parijoto (Medinilla speciosa) has a wide range of pharmacological activities due to its secondary metabolite content, especially flavonoid, and phenolic compounds. Flavonoid and phenolic compounds have a major role in human health with their activities as antioxidants, anti-inflammatories, antimicrobials, etc. This study aimed to determine the total levels of phenolic and flavonoid compounds from the ethanol extract of Parijoto fruit. Parijoto fruit is obtained from Bandungan, Semarang Regency, Central Java. Extraction was carried out by maceration method with 70% ethanol solvent then concentrated using a rotary evaporator. The total phenolic and flavonoid content amount was determined using the colorimetric method with Folin-ciocalteau complex reagent on phenolic with gallic acid as the reference standard and AlCl3 complex reagent on flavonoids with quercetin as the reference standard. Absorbance was measured using a UV-Vis spectrophotometer. The results showed that the color of the viscous extract of Parijoto fruit was dark brown with a yield of 6.67% w/w. The total phenolic content of the ethanol extract of Parijoto fruit was 21.67 µgGAE/g extract and the total flavonoid content was 9.21 µg QE/g extract.

Hepatoprotective Effect of Parijoto Fruit Extract (Medinilla speciosa Blume) on Male Mice Fed with High-Fat Diet

This study aims to determine the effectiveness of parijoto fruit extract (Medinilla speciosa Blume) in improving the condition of fatty liver in male balb/c mice (Mus musculus L.) fed with a high-fat diet. Thirty male balb/c mice weighing 20 to 30 g were randomly divided into six groups, i.e: 1) Standard feed and Carboxymethyl Cellulose Sodium or CMC-Na 0.5% (K1); 2) High-fat diet and CMC-Na 0.5% (K2); 3) High-fat diet and simvastatin 0.026 mg/day (K3); 4) High-fat diet and parijoto fruit extract 5.6 mg/20 g BW (P1); 5) High-fat diet and parijoto fruit extract 8.4 mg/20 g BW (P2); 6) High-fat diet and parijoto fruit extract 11.2 mg/20 g BW (P3). Standard feed, high-fat diet, simvastatin, and parijoto fruit extract were administered for 56 days. On the 57th day, the total of 30 mice were terminated and the livers were then removed for H&E staining histopathological slides. Data on the degree of fatty liver on histopathological slides were collected and analyzed using the Kruskal-Wallis test and followed by the Mann-Whitney test. The histopathological analysis showed Medinilla speciosa Blume extract at a dose of 5.6 mg/20 g BW in group P1 prevent the steatosis degree compared to high-fat feed mice in group K2 (p>0.05). Parijoto fruit extract could act as the potential treatment for fatty liver.

Effects of An Isotonic Medinilla Speciosa During and After Exercise

Effects of An Isotonic Medinilla Speciosa During and After Exercise

Alginate-Based Nanoencapsulation on Ultrasonic-Assisted Extraction of Parijoto Fruit (Medinilla Speciosa Blume) and Its Antioxidant Activity

Parijoto (Medinilla speciosa Blume) has very strong antioxidant activity, but the bioavailability was low. Therefore, Parijoto should be formed into a nanoparticle. The research aimed to determine the characterization and IC₅₀ value of nanoencapsulated Parijoto. Encapsulation was done using ionic gelation with alginate: CaCl₂ ratio 0.05%:0.05% (b/v). The ultrasonication was modified by a variation in frequency and sonication time. The characterization of nanoparticles was carried out using PSA to show the particle size and polydispersity index (pdI), and UV-Vis Spectrophotometer to show the percent of transmittance. The antioxidant activity was determined using FRAP assay. The characterization of pre-sonicated nanoextract was 265 nm of particle size, 0,472 of PdI, and 98,29% of transmittance. The best condition of sonication effect is given from 45 Hz of frequency and 15 minutes in time. The lower particle size from sonicated nanoextract was 218 nm, 0,415 of PdI, 99,56% percent of transmittance, and IC50 value obtained 1.696±0,014 ppm with a very strong category.

Cellular Mechanism of the Cytotoxic Effects of Medinilla speciosa (Parijoto) Methanol Extract on MCF-7/HER-2 Cells is Through Increased Caspase-9 Protein and Decreased Vascular Endothelial Growth Factor Expression

a peer-reviewed, open access online international journal which publishes original research papers. The journal welcomes submission from scholars and experts for possible publication from all over the world. The scope of the journal includes: Pharmaceutical research, chemistry and biochemistry of naturally occurring compounds, biological evaluation of crude extracts, ethnomedicine, traditional and complementary medicine, ethnopharmacology, biomedical research, Biotechnology, Evaluation of natural substances of land and sea and of plants, microbes and animals, pharmacognosy, bioavailability, clinical, pharmacological, toxicological studies and pharmacokinetics of phytochemicals, Isolation and characterization of compounds, structure elucidation, synthesis and experimental biosynthesis of natural Product as well as developments of methods in these areas are welcomed in the journal.

Cytotoxic effects of parijoto (Medinilla speciosa Reinw. Ex. Bl.) methanol extract combined with cisplatin on WiDr colon cancer cells through apoptosis induction

Parijoto (Medinilla speciosa Reinw. Ex. Bl.) is a medicinal plant with cytotoxic effects on cancer cells in vitro. As only a limited number of studies have reported the effect of parijoto on colon cancer cells, this study initially aimed to measure the total flavonoid levels and potential cytotoxic effects of parijoto methanol extract (PME) through cell viability assays and expression of the apoptotic protein on WiDr colon cancer cells as a model. PME cytotoxic activity was determined by conducting a cytotoxicity test on WiDr colon cancer cells using the MTT assay. The synergistic cytotoxic effects of the PME and cisplatin were tested to obtain the combination index (CI) value. Apoptosis was analyzed by flow cytometry, and the apoptotic protein expression was observed by immunocytochemical tests. Furthermore, quercetin as a major flavonoid in PME was measured using a UV–Vis spectrophotometer. The results showed that PME had a moderate cytotoxic activity with an IC50 of 198.64±1.6 µg/mL, whereas the IC50 of cisplatin was 2.34±0.7 µg/mL. The PME with cisplatin combination test showed a strong synergistic effect with a CI value of <1 (0.1‐0.4). The combination showed increased apoptosis properties compared to PME treatment alone. In addition, immunocytochemistry showed that PME alone or in combination with cisplatin increased the pro‐apoptosis proteins (p53 and caspase‐9) and suppressed Bcl‐2 expression. Moreover, the cell viability value increased as the PME concentration decreased. The administration of PME led to changes in cell morphology, lower cell density, and a decreasing number of living cells. Therefore, the combination of PME and cisplatin had a strong synergistic effect in inducing apoptosis.

REVIEW ARTICLE: AKTIVITAS FARMAKOLOGI TANAMAN PARIJOTO (Medinilla speciosa)

Indonesia has a variety of plants that have potential as medicinal ingredients. One of the plants that can be used as medicine for various diseases is the parijoto plant. Parijoto is a plant that belongs to the Melastomataceae family. Parijoto plants contain flavonoid compounds, saponins, tannins and terpenoids. The content of compounds contained in the parijoto plant has various pharmacological activities. This study aims to determine the pharmacological activity of the parijoto plant (Medinilla speciosa). The method used in this study is to use the literacy review method. Reference search is done by searching Google search, Google Scholar, PubMed according to the topic. Based on a review of several literatures, it is shown that the parijoto plant (Medinilla speciosa) has pharmacological activity as antibacterial, antibiofilm, antidiabetic, antifungal, antioxidant, anticholesterol, and cytotoxic.

Copigmentation of Anthocyanin Extract from Parijoto Fruit (Medinilla speciosa) and Its Stability at Different Temperatures and Heating Durations

Parijoto fruit (Medinilla speciosa) has a red color and contains anthocyanins. Anthocyanins have low stability due to the effect of heating. Copigmentation can be applied to improve the stability of anthocyanins. This research aims to study the effect of the anthocyanin extract from parijoto fruit ratio and copigment on anthocyanin concentration and color stability during heating at different temperatures and durations. Anthocyanin copigmentation using tannic acid in a ratio of 1:20 and 1:40 with control without copigmentation (1:0) showed that increasing the tannic acid copigment would increase anthocyanin concentrations and reduce anthocyanin losses due to increased temperature and heating time. The use of 1:40 tannic acid copigment increased anthocyanin concentration by 38%. In addition, increasing the concentration of tannic acid increased the ability of anthocyanins to maintain color retention during heating and increased temperatures. The use of tannic acid at a ratio of 1:40 only decreased color retention by 16%, compared to control (54%) after heating at 75ºC heating for 120 minutes. Copigmentation with tannic acid up to a ratio of 1:40 was able to maintain the stability of the lightness (L*), the reddish value (a*), and the yellowish value (b*) of the anthocyanins. This study showed that this system is potential for food coloring application in the food industry.

Characterization and antimicrobial activity of gold nanoparticles fruit infusion of Medinilla speciosa

Parijoto (Medinilla speciosa) is one of plants known to have antibacterial activity that can be used as bioreductor. The purpose of this study was to determine the antibacterial activity of gold nanoparticles using Parijoto as bioreductor (AuNPsP) and to determine the concentration that had the highest antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus. This research was conducted to prepare gold nanoparticles using reductant infusion of parijoto fruits, with a ratio of reducing agent and gold solution 1:4, 1:6, and 1:8 respectively. Characterization of gold nanoparticles was carried out using UV Vis Spectrophotometer, Particle Size Analyzer (PSA), Fourier Transformed Infrared (FTIR) Spectrophotometer, Transmission Electron Microscope (TEM) then tested for antibacterial investigation against Pseudomonas aeruginosa and Staphylococcus aureus. The morphology, size, and structural properties of synthesized gold nanoparticles were determined with TEM and PSA analysis which showed spherical mono-dispersed structure with an average particle size of 200-450 nm. FTIR analysis reveal that are responsible for the capping and stabilization of synthesized gold nanoparticles. The effectiveness of AuNPsP against Pseudomonas aeruginosa and Staphylococcus aureus increases as the concentration of gold nanoparticle increases. Therefore, the antibacterial investigation revealed that the AuNPsP exhibited good antimicrobial activity.

The Diversity, Distribution and Conservation of Bali’s Medinilla in Eka Karya Bali Botanical Garden

Medinilla is a tropical forest plant that has potential as an ornamental and medicinal plant. The diversity of medinilla in Bali is poorly studied. This study aims to determine the diversity, distribution and conservation activities of Bali’s medinilla at the Eka Karya Bali Botanical Garden. An observational study, documentation and literature were used in this research. Based on this research, it is known that there are six species of medinilla in Bali such as Medinilla crassifolia (Reinw.ex Blume) Blume, Medinilla alpestris Blume, Medinilla laurifolia (Blume) Blume, Medinilla radicans (Blume) Blume, Medinilla speciosa (Reinw.ex Blume) Blume and Medinilla quadrifolia (BI.) Bakh. F. M. quadrifolia is a new distribution record for Bali. All species were discovered in the districts of Buleleng, Bangli, Gianyar, Jembrana, Karangasem and Tabanan districts. Only four species have been conserved in the Bali Botanical Gardens, according to data from the Eka Karya Bali Botanical Gardens' collection, therefore exploration and collection activities are critical. Conservation activities carried out at Eka Karya Bali Botanical Garden are collecting and data collection, maintaining and propagating species of medinilla.

The Diversity, Distribution and Conservation of Bali’s Medinilla in Eka Karya Bali Botanical Garden

Medinilla is a tropical forest plant that has potential as an ornamental and medicinal plant. The diversity of Medinilla in Bali is poorly studied. This study aims to determine the diversity, distribution and conservation activities of Bali’s Medinilla at the Eka Karya Bali Botanical Garden. The research method used are literature study, search for data collection and exploration, amd herbarium observations. Based on this research, it is known that there are six species of Medinilla in Bali such as Medinilla crassifolia (Reinw.ex Blume) Blume, Medinilla alpestris Blume, Medinilla laurifolia (Blume) Blume, Medinilla radicans (Blume) Blume, Medinilla speciosa (Reinw.ex Blume) Blume and Medinilla quadrifolia (BI.) Bakh. F. M. quadrifolia is a new distribution record for Bali. All species were discovered in the districts of Buleleng, Bangli, Gianyar, Jembrana, Karangasem and Tabanan districts. Only four species have been conserved in the Bali Botanical Gardens, according to data from the Eka Karya Bali Botanical Gardens' collection, therefore exploration and collection activities are critical. Conservation activities carried out at Eka Karya Bali Botanical Garden are collecting and data collection, maintaining and propagating species of Medinilla.

Effect of Methanol Extract, Methanol Fraction, and N-Hexane Fraction of Parijoto Fruit (Medinilla speciosa) on Seminal Parameters and Testicular Weight of Male Sprague Dawley Rats

Infertility occurs in 2 million couples or 17% of couples who are married more than 2 years but are not pregnant or have signs of pregnancy. In couples who do not have children, 50% of male infertility factors are caused by abnormalities in semen. Empirically, parijoto fruits (Medinilla speciosa Blume) are used by the people of Kudus, Central Java, Indonesia to increase fertility. The purpose of this research was to find out the effect of methanol extract, methanol fraction, and n-hexane fraction of parijoto fruit (Medinilla speciosa) on seminal parameters and testicular weight of male Sprague Dawley rats. This research used 36 two-month-old Sprague Dawley rats with 200-300 gram of body weight which were divided into 6 groups. Group 1 (normal); groups 2, 3, and 4, used parijoto fruit methanol extract at 100mg/kgBW, 250mg/kgBW, and 500mg/kgBW doses respectively; groups 5 and 6 used methanol fraction and n-hexane fraction of parijoto fruit at 500 mg/kgBW dose respectively for 14 days. Rats were dissected and had examinations on sperm motility, morphology, abd testicular weight. The data were analyzed using Kruskal Wallis and Mann Whitney. The results found that groups 1, 2, 3, 4, 5 and 6 had mean spermatozoa motility (%) that were 56.5±2.43, 72.5±6.89, 77.6±12.99, 83.3±7.53, 84.7±3.98, and 74.2±11.58, mean spermatozoa morphology (%) that were 95.5±3.67, 95±2.76, 92.6±5.13, 96.5±3.27, 94.3±4.37, and 93.2±6.11, and mean testicular weight (gram) that were 1.08±0.10, 0.90±0.14, 0.98±0.10, 1.18±0.21, 1.28±0.43, and 1.02±0.13. There were significant differences between the normal group and all treatments (P <0.05) on spermatozoa motility. There was no significant difference on spermatozoa morphology and testicular weight. Based on the results, it can be concluded that methanol extract, methanol fraction, and n-hexane fraction of parijoto fruit can increase spermatozoa motility of male Sprague Dawley rats.

Pest and Disease Identification in Parijoto Plant (Medinilla speciosa blume) at Nglurah Tawangmangu

Parijoto (Medinilla speciosa blume) is a tropical plant that has purplish pink fruit often called Asian grape. In Indonesia, parijoto plants are very well known as ornamental plants that have various benefits, from parijoto fruit can be used as anti-inflammatory drugs so that ornamental plants are widely cultivated by the community, especially lovers of ornamental plants and ornamental plant cultivation in Nglurah. Medinilla speciosa blume is very suitable in Indonesia, which has a tropical climate, but the high rainfall in Indonesia in the rainy season should get more attention in caring for it. This makes parijoto plants susceptible to several types of diseases and pests that can damage the beauty of the Medinilla speciosa blume plant. However, the lack of experts in this field makes it very difficult for plant lovers and the cultivation community of Medinilla speciosa blume in Nglurah to solve the problem of diseases and pests that attack the Medinilla speciosa blume plant. The purpose of this study was to determine pests and diseases on Medinilla speciosa blume. This research was conducted in Nglurah Village Rt 01 Rw 02 Tawangmangu for five days on 04 – 08 September 2021. This research uses observation and interview methods. Data were analyzed by qualitative descriptive. This study used 10 samples of the observed Medinilla speciosa blume plant. The tools used are cellphone cameras and stationery. The variables to be observed are pests and diseases. The results of this study found 4 pests and 2 diseases on Medinilla speciosa blume including Fussarium oxysporum, Cercospora sp and Pestalotia sp, mealybugs, caterpillars, snails, grasshoppers. For this reason, the solution for treating parijoto plants to avoid pests and diseases requires extra care such as paying attention to soil moisture, giving appropriate fertilizers, and giving vitamins so that parijoto plants can grow well.