Medicinal Importance Research Articles

The medico-legal death investigation system in Nepal.

In Nepal, police inquests have several limitations. When they receive information about a death, the police visit the crime scene and write an inquest report. Then, they arrange for the body to be autopsied. However, most autopsies are performed by medical officers in government hospitals who lack specialised training in conducting autopsies. Although all Nepalese medical schools teach forensic medicine to undergraduate students and require them to witness some autopsies, most private institutions are not authorised to perform them. Autopsies performed without expertise can be subpar and, even when trained personnel are available, these facilities are inadequately equipped. In addition, there is a lack of sufficient manpower to provide expert medico-legal services. The Honourable Judges and District Attorneys of all district courts believe that the medico-legal reports prepared by the doctors are inappropriate, incomplete and inadequate for use as evidence in court. Moreover, the police are more concerned with establishing criminality than other aspects of medico-legal death investigation, such as autopsies. Therefore, the quality of medico-legal investigations, including death investigations, will not improve until government stakeholders recognise the importance of forensic medicine in the judiciary and for the resolution of crimes.

Health Benefits, Therapeutic Applications, and Recent Advances of Cirsilineol in the Medicine: Potential Bioactive Natural Flavonoids of Genus Artemisia.

Herbal medicine and its derived products have been used in the medicine and nutraceutical sectors for the treatment of human disorders and associated secondary complications. Plant-derived products play an important role in our daily life due to their medicinal properties and pharmacological activities. The attention of scientists to natural products has increased due to their significant biological activities. Flavonoids represent one of the most important phytocompounds present in the higher plants, common fruits, vegetables, herbs, wine, juices, and dried fruits. Flavonoids exert potent antioxidant activity by blocking and scavenging free radicals. Cirsilineol, also called 4',5-dihydroxy-3',6,7-trimethoxyflavone, is an active phytochemical of Artemisia vestita, Artemisia monosperma, Artemisia asiatica, and Agrostis gigantea. Medicinal importance and pharmacological activities of cirsilineol have been investigated in the present work with their analytical aspects in order to know the biological importance of cirsilineol in medicine. Literature data on cirsilineol were collected and analyzed in the present work to study its therapeutic potential against various human disorders and associated secondary complications. Scientific data were collected from Google, Google Scholar, PubMed, Science Direct, and Scopus and analyzed in the present work using the term herbal medicine, flavonoid and cirsilineol. Medicinal plants containing a significant amount of cirsilineol have biological applications in medicine due to their pharmacological activities. This present work signifies the biological importance of cirsilineol in medicine as it has antiproliferative, gastroprotective, anti-Helicobacter pylori, anti-diabetic and anti-oxidant activities. Further therapeutic effectiveness of cirsilineol against different types of cancers, including breast carcinoma and lung carcinoma, has been discussed in the present work. The biological importance of cirsilineol against allergic rhinitis, inflammation, coronavirus, immune system, renal cellular membrane and protein glycation has also been discussed in the present work. However, the importance of analytical methods for the isolation and identification of cirsilineol in medicine has also been analyzed. This work aimed to summarize the health-beneficial aspects of cirsilineol in medicine which will be beneficial to explore the further therapeutic effectiveness of cirsilineol for the treatment of various forms of human disorders.

Gut Microbiota and Aging: Traditional Chinese Medicine and Modern Medicine.

The changing composition of gut microbiota, much like aging, accompanies people throughout their lives, and the inextricable relationship between both has recently attracted extensive attention as well. Modern medical research has revealed that a series of changes in gut microbiota are involved in the aging process of organisms, which may be because gut microbiota modulates aging-related changes related to innate immunity and cognitive function. At present, there is no definite and effective method to delay aging. However, Nobel laureate Tu Youyou's research on artemisinin has inspired researchers to study the importance of Traditional Chinese Medicine (TCM). TCM, as an ancient alternative medicine, has unique advantages in preventive health care and in treating diseases as it already has formed an independent understanding of the aging system. TCM practitioners believe that the mechanism of aging is mainly deficiency, and pathological states such as blood stasis, qi stagnation and phlegm coagulation can exacerbate the process of aging, which involves a series of organs, including the brain, kidney, heart, liver and spleen. Our current understanding of aging has led us to realise that TCM can indeed make some beneficial changes, such as the improvement of cognitive impairment. However, due to the multi-component and multi-target nature of TCM, the exploration of its mechanism of action has become extremely complex. While analysing the relationship between gut microbiota and aging, this review explores the similarities and differences in treatment methods and mechanisms between TCM and Modern Medicine, in order to explore a new approach that combines TCM and Modern Medicine to regulate gut microbiota, improve immunity and delay aging.

The importance of military medicine in the early stage of anaesthesiology and resuscitation training in Spain.

The importance of military medicine in the early stage of anaesthesiology and resuscitation training in Spain.

A Narrative Review of Benzo-Fused Coumarins, Shedding Light on Their Medicinal Activities

Background: Coumarins and their derivatives excel in chemical diversity, coupled with a wide variety of biological potentials, and tend to be beneficial to the health of the human body. One of these derivatives that has sparked the attention of medicinal chemistry specialists in recent decades is benzo-fused coumarins, which have demonstrated their potential as antioxidants, antimicrobials, antidiabetics, antithrombotics, and many more. Objective: This paper discusses the medicinal importance of benzo-fused coumarins derived from natural or synthetic sources. Conclusion: The researchers documented that benzo-fused coumarin's basic structure is a promising framework that opens up the chance of discovering innovative applicants with advanced therapeutic potentials.

DNA-Based Molecular Markers and Antioxidant Properties to Study Genetic Diversity and Relationship Assessment in Blueberries

Blueberries (Vaccinium L. spp.) are an economically and medicinally important plant. Their antioxidant properties are well-known for their medicinal value in negating the harmful effects of free radicals. It is very important to develop genotypes that are high in health-promoting factors and economic value to meet present world needs. Estimation of genetic diversity using molecular markers, antioxidant properties, and their association can reveal genotypes with important characteristics and help in berry improvement programs. Wild blueberries are a better source of antioxidant metabolites compared to cultivated ones. Extensive variations are present in molecular and biochemical contents among wild clones and cultivars. The current review provides detailed and updated information on the economic and medicinal importance of blueberries, the application of molecular markers, and biochemical estimation in berry improvement and conservation, filling the gap in the literature.

Medicinal Utility, Nutrition and Importance of Pulses

Medicinal Utility, Nutrition and Importance of Pulses

Traditional Knowledge on the use of Turtles in a Protected Area of the Amazon in Maranhão (Brazil): A Conservation Proposal

The use of turtles for food or breeding is a traditional practice in the Baixada Maranhense Environmental Protection Area (EPA), which is composed of wetlands of high conservation value. However, species such as Kinosternon scorpioides, a semi-aquatic Amazon turtle , are threatened by direct harvesting and habitat loss. Local knowledge of biodiversity has been used to understand these practices and to control these problems as a theoretical basis in research on the use and management of natural resources and conservation. This work aimed to analyze the traditional knowledge of riverside communities on the biology, habitat, capture methods, forms of use, and conservation of turtles in the Baixada Maranhense. Data were collected through semi-structured interviews with inhabitants of riverside communities. Based on our results, turtle species are commonly caught in fishing activities (54.71 percent of the interviewees) or by burning (32.35 percent), a practice with great environmental impacts. 41.77 percent of respondents capture these animals for consumption and prefer the K scorpioides, which also has cultural and medicinal importance for the riverside communities. 89 percent of the interviewees report no knowledge of either the structure and function of an EPA or how resource management works, compromising the sustainability principles and operation of this Conservation Unit (C.U.). It is concluded that capture of turtles is one of the reasons for the decrease in natural stocks of K scorpioides. Strategies for management and conservation of the Baixada Maranhense EPA resources are proposed.

Screening of Phytochemical Compounds in Medicinal Plants

Some plants have good medicinal and therapeutic importance for healthy lifestyle.(8) One among them is Phyllanthus emblica (Linn) belongs to Euphorbeaceae family, commonly known as Amla which has superior value in traditional system of medicine. It has several pharmacological properties, mainly antioxidant activity and anti-inflammatory activity. (Jamwal. K. S. et. al.) The study is focused on phytochemical investigation. The extracts were used to detect the presence of alkaloids, phenols, flavonoids, terpenoids, steroids and other phytochemicals. Antioxidant assay was performed as DPPH radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl). DPPH reacts with the free radicals and change its colour. The basil leaf (occimum basilium van thyrsiflouruae): It contains various compounds such as flavinoids, alkaloid, phenol and essential oil. So, it needs to be fractionalised to find out the chemical substance but have a definite physiological action on the living system. The qualitative analysis is very essential to the phytochemical constituents present in medicinal plants.(Habib- ur Rehman. K.A.; et. al.) The basil leaves of phytochemical analysis revealed the presence of several bio active components such as flavinoids, phenols, alkaloids, tannins, steroids and saponin. (7) 21st March 1997 - It is native to asia (India, Pakistan, Thailand and other countries) Phyllanthus niruri: We have selected Phyllanthus niruri commonly known as keezha nelli. It is an important ethno-botanical species of India and widely used in Ayurveda formulations. Plant derived immunostimulants are a promising alternative to chemotherapeutics and also perhaps vaccines.

Synthesis, structure-activity relationships and biological evaluation of benzimidazole derived sulfonylurea analogues as a new class of antagonists of P2Y1 receptor.

The P2Y receptors are responsible for the regulation of various physiological processes including neurotransmission and inflammatory responses. These receptors are also considered as novel potential therapeutic targets for prevention and treatment of thrombosis, neurological disorders, pain, cardiac diseases and cancer. Previously, number of P2Y receptor antagonists has been investigated but they are less potent and non-selective with poor solubility profile. Herein, we present the synthesis of new class of benzimidazole derived sulfonylureas (1a-y) as potent antagonists of P2Y receptors, with the specific aim to explore selective antagonists of P2Y1 receptors. The efficacy and selectivity of the synthesized derivatives 1) against four P2Y receptors i.e., t-P2Y1, h-P2Y2, h-P2Y4, and r-P2Y6Rs was carried out by calcium mobilization assay. The results revealed that except 1b, 1d, 1l, 1m, 1o, 1u, 1v, 1w, and 1y, rest of the synthesized derivatives exhibited moderate to excellent inhibitory potential against P2Y1 receptors. Among the potent antagonists, derivative 1h depicted the maximum inhibition of P2Y1 receptor in calcium signalling assay, with an IC50 value of 0.19 ± 0.04µM. The potential of inhibition was validated by computational investigations where bonding and non-bonding interactions between ligand and targeted receptor further strengthen the study. The best identified derivative 1h revealed the same binding mechanism as that of already reported selective antagonist of P2Y1 receptor i.e (1-(2- (2-tert-butyl-phenoxy) pyridin-3-yl)-3-4-(trifluoromethoxy) phenylurea but the newly synthesized derivative exhibited better solubility profile. Hence, this derivative can be used as lead candidate for the synthesis of more potential antagonist with much better solubility profile and medicinal importance.

ASSESSMENT OF THE STRESS AND METABOLISM LEVELS BY USING ELECTRO-PHOTONIC EMISSION ANALYSIS METHOD IN UKRAINIAN MILITARY PERSONNEL AFTER FRONTLINE SERVICE

This study aims to explore the potential of the Electrophotonic Emission Analysis (EPEA) method as an instrumental approach for studying stress in biomedical research. Specifically, the study focuses on assessing the stress levels of Ukrainian military personnel returning from the front using the EPEA method. Materials and methods: A total of 96 individuals participated in this open, non-randomized, exploratory, non-controlled, comparative study. Among them, 26 were military personnel, while the rest belonged to the comparison groups. The EPEA analysis was conducted using a certified hardware and software measuring device called Bio-Well 2.0 (United States). Results: The study found a significant decrease (p<0.0001) in energy levels, with both the military personnel group (41±4.25 J) and the functionally healthy individuals group (57.38±20.78 J) showing similar results. Among the participants, 62% of military personnel and 95% of patients with non-communicable diseases demonstrated optimal energy levels, while 38% and 5% respectively showed reductions. The stress levels measured were 3.69±0.62 cu for military personnel, 3.69±0.62 cu for functionally healthy individuals, and 3.99±1.17 cu for patients with non-communicable diseases. Conclusions: The EPEA method proved effective in instrumentally detecting increased levels of stress and anxiety in 96% of military personnel, 93% of functionally healthy individuals, and 87% of patients with non-communicable diseases in the study. It is of fundamental importance for scientific and systemic medicine to establish that changes in biophoton emission can be observed in patients with non-communicable diseases, including military personnel. Significant differences in total energy levels were found between patients and functionally healthy individuals, with EPEA results showing 41±4.25 J and 43.86±4.87 J respectively for patients and functionally healthy people (p<0.0001). Electro-photonic emission analysis is a promising, valid, accessible instrumental method for biomedical research.

The effects of nature-inspired amino acid substitutions on structural and biochemical properties of the E. coli L-asparaginase EcAIII.

The Escherichia coli enzyme EcAIII catalyzes the hydrolysis of L-Asn to L-Asp and ammonia. Using a nature-inspired mutagenesis approach, we designed and produced five new EcAIII variants (M200I, M200L, M200K, M200T, M200W). The modified proteins were characterized by spectroscopic and crystallographic methods. All new variants were enzymatically active, confirming that the applied mutagenesis procedure has been successful. The determined crystal structures revealed new conformational states of the EcAIII molecule carrying the M200W mutation and allowed a high-resolution observation of an acyl-enzyme intermediate with the M200L mutant. In addition, we performed structure prediction, substrate docking, and molecular dynamics simulations for 25 selected bacterial orthologs of EcAIII, to gain insights into how mutations at the M200 residue affect the active site and substrate binding mode. This comprehensive strategy, including both experimental and computational methods, can be used to guide further enzyme engineering and can be applied to the study of other proteins of medicinal or biotechnological importance.

Phytochemical content and antioxidant activity of different anatomical parts of Corchorus olitorius and C. capsularis during different phenological stages

Different parts of Corchorus olitorius and C. capsularis possess different antioxidant compounds. This study investigated the phytochemical components and antioxidant capacities of ultrasound-assisted extraction of different plant parts of both species using spectrophotometry at various phenological stages. Results also indicate that leaves, stems and roots of C. olitorius at various growth stages showed higher phytochemical components and antioxidant potential compared to C. capsularis. The phytochemical components from roots to leaves in C. olitorius including total polyphenol 0.97–11.11 mg GAE/g DW, total flavonoid 0.99–7.78 mg QE/g DW and total tannin 4.02–26.89 TA E/g DW, whereas C. capsularis total polyphenol 1.04–7.93 mg GAE/g DW, total flavonoid 0.77–5.5.92 mg QE/g DW and total tannin content 3.17–22.73 TA E/g DW. C. olitorius produced overall 22.23%, 13.61%, 12.24% higher total polyphenol, total flavonoid and total tannin, respectively compare to C. capsularis. Different parts extract also significantly affected antioxidant capacities including DPPH, ABTS, and FRAP activity with values of 22.03–79.46% inhibition, 10.84–104.10 μmol TE/g DW, and 10.84–104.10 μmol Fe2+/g DW respectively for C. olitorius, while C. capsularis demonstrated 14.03–70.97% of DPPH inhibition, 9.16–95.60 μmol TE/g DW of ABTS and 5.31–71.82 μmol Fe2+/g DW of FRAP activity. Moreover, leaves of the flowering stage, young stems and aged roots of both species displayed a higher content of phytochemical and antioxidant activities than other growth stages. A positive correlation between the phytochemical and antioxidant potential indicated that phenolic constituents solely affected antioxidant activity. Thus, this study established that the plant's parts and phenological growth stages significantly influence the concentration of phytoconstituents and antioxidant activities, and determine the harvesting stages of the different organs of C. olitorius and C. capsularis for considerable medicinal importance as folk and industry.

The Promises and Pitfalls of CRISPR-Mediated Base Editing in Stem Cells.

Stem cells such as induced pluripotent stem cells, embryonic stem cells, and hematopoietic stem and progenitor cells are growing in importance in disease modeling and regenerative medicine. The applications of CRISPR-based gene editing to create a mélange of disease and nondisease stem cell lines have further enhanced the utility of this innately versatile group of cells in the studies of human genetic disorders. Precise base edits can be achieved using a variety of CRISPR-centric approaches, particularly homology-directed repair and the recently developed base editors and prime editors. Despite its much-touted potential, editing single DNA bases is technically challenging. In this review, we discuss the strategies for achieving exact base edits in the creation of various stem cell-based models for use in elucidating disease mechanisms and assessing drug efficacy, and the unique characteristics of stem cells that warrant special considerations.

İsrail Akut Arı Felci Virüsü’nün Türkiye’de Koloni Kayıplı Arılıklardaki Yaygınlığı

Honeybees are indispensable pollinator insects for vegetative pollination and biodiversity. Moreover, they serve medicinal importance with products such as honey, propolis, pollen, and royal jelly. Sudden bee deaths and colony collapse disorder (CCD) threaten the sustainability of colony health. Honeybee viruses, parasites, and pathogens trigger colony losses and CCD. This study investigated the presence and prevalence of Israeli acute bee paralysis virus (IAPV) in apiaries with sudden bee deaths, colony losses, and CCD-like problems in 16 provinces in different eco-geographic regions of Türkiye between 2011- 2021. Samples were tested for the coexistence of honeybee pathogens with IAPV. The sampled apiaries were evaluated for other bee pathogens such as Acute bee paralysis virus, Black queen bee virus, Chronic bee paralysis virus, Deformed wing virus, Kashmir bee virus, Lake Sinai virus, Sacbrood virus, Varroa mites, and Nosema sp. analyzed. Pathogen-specific RT-PCR assay was used for bee viruses. IAPV positivity was found to be 52.5% in apiaries. 97.5% of the sampled apiaries were positive for at least one pathogen. According to the results of this study, the presence of IAPV in apiaries suffering from colony loss and CCD-like problems was higher than in previous reports, and viruses of different species, Nosema sp., and varroa infestation were found to be frequently encountered. The results suggest that the coexistence of IAPV and multiple pathogens may be effective in colony losses.

Impact of exogenous salicylic acid and hydrogen peroxide on vitamins, essential nutrients and silymarin content of milk thistle grown at two different altitudes of balochistan under cadmium stress

Cadmium (Cd+2), a potentially toxic element, inhibits growth and development of several plant species, especially medicinal plants. However, exogenous supplementation of various hormones, compounds and signaling molecules helps to mitigate the impact of Cd+2 toxicity. Medicinal value of plants in today's era cannot be undermined. Silybum marianum (L.) Gaertn. (milk thistle) is one of most important and valuable medicinal plant; its silymarin content is widely being used to treat liver diseases. The present study was conducted during October 2019–March 2020 to analyze the potential impact of exogenous supplementation with 0.25 μM salicylic acid (SA) and 10 μM hydrogen peroxide (H2O2) priming, foliar and their combinational treatments on various attributes of milk thistle at two diverse ecological zones of Balochistan province of Pakistan, i.e., Quetta (Qta) and Turbat (Tbt) under Cd+2 toxicity. So far, no study conducted considering these two ecological zones of Pakistan with respect to cadmium toxicity on milk thistle nutritional and physio-biochemical attributes. The design of the experiment was randomized complete block design (RCBD) under factorial with three replication each treatment. Results portrayed that SA and H2O2, priming, foliar spray and combinational treatments were quite effective in alleviating the negative impacts of Cd+2 toxicity. The essential nutrients, i.e., nitrate (NO3−), calcium (Ca2+), potassium (K+) and vitamins viz., ascorbic acid (AsA), niacin and riboflavin were affected by Cd+2 induced toxicity; however, the substantial impact of SA and H2O2 helped to reduce the Cd+2 toxicity and boosted up plant nutrients vitamin content. In a nutshell, exogenous treatments of SA and H2O2 enhanced the yield potential by increasing the weight of hundred seeds, enhancing the accumulation of silymarin content in milk thistle seeds which is of prime significance for its medicinal importance in treatment of liver diseases. Therefore, the data obtained in this study highly recommends priming (P) and foliar (F) spray along with combinational treatment of SA and H2O2 as the best strategy to ameliorate Cd+2 toxicity in plants, which will ultimately lead to better growth and yield of the plants.

In Vitro Thrombolytic Activity Test On Ethyl Acetate Fraction of Herb Phyllanthus niruri L

Atherothrombotic, which is an atherosclerotic inflammation with thrombus, is characterized by damage to blood vessels that causes myocardial infarction and isemic stroke with thrombolytic treatment. The side effects of thrombolytic treatment are the cause of the importance of alternative medicine from natural ingredients. The ethyl acetate fraction in Phyllanthus niruri L. is suspected to have thrombolytic activity due to the content of coumarin and flavonoid bioactive compounds based on research that has been done. The purpose of this study was to determine the thrombolytic activity of the ethyl acetate fraction of Phyllanthus niruri L. Thrombolytic activity test is done by measuring the percentage of clot lysis. This study used a true experimental method with a Post Test Only Control Group Design model in 6 treatments, positive control, negative control (PBS and 0.5% DMSO) and four samples for both tests, namely 250 g/mL, 500 g/mL, 1000 g/mL, 2000 g/mL. The results of statistical analysis showed that the data were normally distributed (Shapiro Wilk, p>0.05). The result of the largest clot lysis thrombolytic test was 24.43% (2000ppm) smaller than the positive control 46.33%. The results of the thrombolytic ANOVA test showed that there was no significant difference between treatments, p<0.05, namely 0.017 and F Count > F Table, namely 0.937>3.11. Based on the results, it was found that the ethyl acetate fraction of the herb Phyllanthus niruri L. had a thrombolytic substance with low potency.

Medicinal and Nutritional Importance of Mushrooms

Mushrooms have been used as food for centuries because of their unique taste, in addition to their great nutritional value, as it was found that there are many nutritional and medical benefits due to human consumption of mushrooms, both wild and cultivated. Nutritionally, mushrooms are rich in proteins, carbohydrates and fibers and are also low in energy and fats. It also contains a large variety of nutrients and minerals such as potassium, copper and vitamins such as riboflavin, niacin and folate. Most types of mushrooms are an important source of active compounds with the potential for medicinal and therapeutic effects. Biologically of potential medical importance, as the results of studies showed that the bioactive components isolated from mushrooms have pharmacological effects such as antitumor, antioxidant, antiviral, cholesterol-lowering, as well as lowering blood sugar effects in patients. Consuming mushrooms or one of their products in our daily diet may provide many health benefits.

Biological evaluation and in silico molecular docking studies of Abies cilicica (Antoine & Kotschy) Carrière) resin

Abies cilicica (Antoine & Kotschy) Carrière) is an endemic species of industrial and medicinal importance. The resin obtained from this plant is effective for different diseases. The chemical content of the ethanol extract of Abies cilicica resin by GC-MS method, its antimicrobial activity on 12 different microorganisms by MIC value, antibiofilm activity on six microorganisms by Christensen method and biological activities on three different cancer cell lines by MTT method were investigated in this study. The findings showed that antimicrobial and antibiofilm activity was high in L. monocytogenes. The resin extract showed strong anticancer activity on all three DU-145, HeLa and SaOS-2 cells. The major component of the resin was abietic acid. A molecular docking simulation of the major compounds from resin extracts was conducted. In this study, in which the strong biological properties of A.cilicica resin were revealed, which suggests that the resin can increase the inhibition of cancer cell growth while strengthening the immune system against pathogenic microorganisms. Molecular docking analysis performed supported the experimental observations. Further extensive studies will support the possibility of using this resin as a therapeutic agent.

A Study on Awareness and Attitude of People towards Use of Traditional Medicine in Central Kashmir, J&K

Jammu and Kashmir region popularly known as heaven on earth is covered by loftymountains, dense forests which receive heavy rainfall and snow. The forests in Jammu and Kashmir region arespread over broad geo-climatic zones, play most significant role in the economy of the region as they provide rich forest resources indispensable for human beings. Since ancient times Herbalmedicine, also called as herbalism or botanical medicine, a medical system based on theuse of plants or plant extracts that may be eaten or applied to the skinto treat illness and to assist bodily functions. The Hakeems and the Vaidayas of study area, have rich treasure of knowledge about the local medicinal plants and the system is still used mostly in remoteareas of Jammu and Kashmir region. We know Nomads have their own traditional knowledge of heath care, unfortunately this knowledge is threatened due to various natural and anthropogenic factors. We undertake this study on thisbackground to assess the awareness an awareness among people of central Kashmirof Jammu and Kashmir region. In present study, a well designed validated questionnaire was used to collect the information from a sample of 400 people (200 male and 200 female) selected randomly from different Unani hospitals and in the study area. The data collected from survey was tabulated, analyzed and interpreted statistically using standard statisticalmethods. The results obtained from our survey showed that respondents understudy male as well as female are aware about importance of traditional medicine but lack in attitude towards use of Unani medicine for treatment of illness. The study revealed that majority of respondents (male=69.5%, female= 73.5%) were aware about unani medicine as a mode of treatment to cure various diseases. Further, main source of information about unani medicine among respondents (male=32.5%, female= 38.5%) were elders in family, followed by neighbours (male=17.0%, female= 20.0%) and by friends (male=15.0%, female= 18.5%). Statistically, significant difference was observed between male and female respondents in preference of treatment (P<0.01). It was concluded from our study that lack of attitude towards use of medicinal plants as treatment for illnessand facilities were the majorproblems for the efficient use of Unani medicine. There is an urgent need of giving mass awareness on importance of medicinal plants to the people of the Jammu and Kashmir region.