In Vitro Thrombolytic Activity Test On Ethyl Acetate Fraction of Herb Phyllanthus niruri L

Abstract

Atherothrombotic, which is an atherosclerotic inflammation with thrombus, is characterized by damage to blood vessels that causes myocardial infarction and isemic stroke with thrombolytic treatment. The side effects of thrombolytic treatment are the cause of the importance of alternative medicine from natural ingredients. The ethyl acetate fraction in Phyllanthus niruri L. is suspected to have thrombolytic activity due to the content of coumarin and flavonoid bioactive compounds based on research that has been done. The purpose of this study was to determine the thrombolytic activity of the ethyl acetate fraction of Phyllanthus niruri L. Thrombolytic activity test is done by measuring the percentage of clot lysis. This study used a true experimental method with a Post Test Only Control Group Design model in 6 treatments, positive control, negative control (PBS and 0.5% DMSO) and four samples for both tests, namely 250 g/mL, 500 g/mL, 1000 g/mL, 2000 g/mL. The results of statistical analysis showed that the data were normally distributed (Shapiro Wilk, p>0.05). The result of the largest clot lysis thrombolytic test was 24.43% (2000ppm) smaller than the positive control 46.33%. The results of the thrombolytic ANOVA test showed that there was no significant difference between treatments, p<0.05, namely 0.017 and F Count > F Table, namely 0.937>3.11. Based on the results, it was found that the ethyl acetate fraction of the herb Phyllanthus niruri L. had a thrombolytic substance with low potency.