Medical Plant Extracts Research Articles

A Review: Effective Medical Plant Extracts Used against Parasites

This review aimed to identify most important medical plants extract used to treat parasites human diseases. Parasitic infections can define as any illnesses or cases caused by parasites and reproducing in the body. The parasites are living organisms that need another animal or living thing called (a host) to have nutrients need to survive, protozoa, trematodes, cestodes and nematodes are types of this parasite. Which can affect about 30% of the human population. The medicinal plant is any plant which contains in their organs or tissues substances can be used for therapeutics or became precursors for the synthesis medicinal drugs.

Effect of some Medical Plant Extracts and Chemicals on Hormones of Female Rats

The study was carried out on 45 sexually mature female albino rats at the age of 7-8 weeks and weighing 160-180 ± 10 gm., nine treatments were used in this study, where five rats were isolated for control treatment (without infection). The remaining rats were injected (Subcutaneous) with Indian-made Alloxan, which was prepared at the time of injection at a dose of 100 mg/kg of body weight. The purpose of this study was to determine the effect of extracts of medical plants (Curcumin, Origanum majorana, and Vitex aguns-castus) at 50-100 mg/kg body weight and chemotherapy (metformin at 50 mg/kg body weight) on hormones. The results showed a significant increase in LH hormone in the T3, T4, T5, T7, and T9 treatments compared with the T2 treatment. A significant increase in FSH was observed in T3, T4, T5, T6, T7, and T9 treatments compared with T2 treatment. The results of the statistical analysis indicated a significant increase in progesterone in the T3, T5, T7, and T9 treatments compared with the T2 treatment. While T3, T5, and T9 treatments were significantly superior to T2, T4, T6, T7, and T8 treatments in terms of estrogen hormone. While a significant increase in cortisol was observed in the T2 treatment compared with the rest of the treatments. The aim of this study was to investigate the effect of aqueous extracts of Curcumin, Origanum majorana and Vitex aguns-castus leaves on the sex hormones of female rats. The results have shown that the aqueous extracts have a positive effect on hormones in female rats.

Selective Cytotoxicity of Medical Cannabis (Cannabis sativa L.) Extracts Across the Whole Vegetation Cycle Under Various Hydroponic and Nutritional Treatments.

Introduction: The use of Cannabis sativa L. in health care requires stringent care for the optimal production of the bioactive compounds. However, plant phenotypes and the content of secondary metabolites, such as phytocannabinoids, are strongly influenced by external factors, such as nutrient availability. It has been shown that phytocannabinoids can exhibit selective cytotoxicity against various cancer cell lines while protecting healthy tissue from apoptosis. Research Aim: This study aimed to clarify the cytotoxic effect of cannabis extracts on colorectal cell lines by identifying the main active compounds and determining their abundance and activity across all developmental stages of medical cannabis plants cultivated under hydroponic conditions. Materials and Methods: Dimethyl sulfoxide extracts of medical cannabis plants bearing the genotype classified as chemotype I were analyzed by high-performance liquid chromatography, and their cytotoxic activity was determined by measuring cell viability by methylthiazolyldiphenyl-tetrazolium bromide assay on the human colon cancer cell lines, Caco-2 and HT-29, and the normal human epithelial cell line, CCD 841 CoN. Results: The most abundant phytocannabinoid in cannabis extracts was tetrahydrocannabinolic acid (THCA). Its maximum concentrations were reached from the 7th to the 13th plant vegetation week, depending on the nutritional cycle and treatment. Almost all extracts were cytotoxic to the human colorectal cancer (CRC) cell line HT-29 at lower concentrations than the other cell lines. The phytocannabinoids that most affected the cytotoxicity of individual extracts on HT-29 were cannabigerol, Δ9-tetrahydrocannabinol, cannabidiol, cannabigerolic acid, and THCA. The tested model showed almost 70% influence of these cannabinoids. However, THCA alone influenced the cytotoxicity of individual extracts by nearly 65%. Conclusions: Phytocannabinoid extracts from plants of the THCA-dominant chemotype interacted synergistically and showed selective cytotoxicity against the CRC cell line, HT-29. This positive extract response indicates possible therapeutic value.

Sustainable-Green Synthesis of Silver Nanoparticles Using Aqueous Hyssopus officinalis and Calendula officinalis Extracts and Their Antioxidant and Antibacterial Activities.

Silver nanoparticles (AgNPs) biosynthesized using aqueous medical plant extracts as reducing and capping agents show multiple applicability for bacterial problems. The aim of this study was to expand the boundaries on AgNPs using a novel, low-toxicity, and cost-effective alternative and green approach to the biosynthesis of metallic NPs using Calendula officinalis (Calendula) and Hyssopus officinalis (Hyssopus) aqueous extracts. The formation of AgNPs was confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS) techniques. The effectiveness of biosynthesized AgNPs in quenching free radicals and inhibiting the growth of Gram-positive and Gram-negative microorganisms was supported by in vitro antioxidant activity assay methods and using the Kirby-Bauer disk diffusion susceptibility test, respectively. The elucidated antimicrobial and antioxidative activities of medical plant extracts were compared with data from the engineered biosynthetic AgNPs. The antimicrobial effect of engineered AgNPs against selected test cultures was found to be substantially stronger than for plant extracts used for their synthesis. The analysis of AgNPs by TEM revealed the presence of spherical-shaped nano-objects. The size distribution of AgNPs was found to be plant-type-dependent. The smaller AgNPs were obtained with Hyssopus extract (with a size range of 16.8 ± 5.8 nm compared to 35.7 ± 4.8 nm from Calendula AgNPs). The AgNPs' presumably inherited biological functions of Hyssopus and Calendula medical plants can provide a platform to combat pathogenic bacteria in the era of multi-drug resistance.

Dietary Supplements in Chemotherapy-Induced Peripheral Neuropathy: A New Hope?

Chemotherapy-induced peripheral neuropathy (CIPN) is one of the main and most prevalent side effects of chemotherapy, significantly affecting the quality of life of patients and the course of chemotherapeutic treatment. Nevertheless, despite its prevalence, the management of the CIPN is considered particularly challenging, with this condition often being perceived as very difficult or even impossible to prevent with currently available agents. Therefore, it is imperative to find better options for patients diagnosed with this condition. While the search for the new agents must continue, another opportunity should be taken into consideration—repurposing of the already known medications. As proposed, acetyl-L-carnitine, vitamins (group B and E), extracts of medical plants, including goshajinkigan, curcumin and others, unsaturated fatty acids, as well as the diet composed of so-called “sirtuin-activating foods”, could change the typical way of treatment of CIPN, improve the quality of life of patients and maintain the continuity of chemotherapy. This review summarizes currently available data regarding mentioned above agents and evaluates the rationale behind future research focused on their efficacy in CIPN.

Effects of Gel Extract from Aloe Vera Cultivated in Iraq on Blood Glucose Level and Lipid Profile in Induced Diabetic Mice

In Iraq; there is a great demand for handling some epidemic diseases such as hyperglycemia and hyperlipidema through searching some medical plant extracts which the country is rich with and contains an important phytochemicals that may solve the incidence of such cases. Aloe vera that cultivated in Iraq, is known to be rich with biological active constituents. The aim of the present study; the plant gel extract was subjected to treat an induced diabetic lab mice through injecting streptozotocin dose with high fatty food to elevate lipid profile before induction of the gel oral treatment of the plant. In the study results: the oral administration of A.vera gel at concentration of 300mg/kg animal weight gives a reduction in glucose level reached to 122mg/dl at the end of the experiment as well as the anti-diabetic drug Glibenclamide in a dose of 600µg/kg body weight as the glucose level decreased to 123mg/dl glucose in comparison to glucose level 250 mg/dL at zero time. Also the plant gel shows an effect on lipid profile that include cholesterol, triglycerides and High density lipoprotein levels; the A.vera extract after 21 days treatment causes decreasing in all lipid levels; even in normal mice fed with the extract only, except the High density lipoprotein levels had no change after the extract treatment than the negative control.

Study of Antimicrobial Activity of Few Medicinal Plants Extracts on Growth of Helicobacter Pylori

Background: H. pylori is a microaerophilic Gram-negative bacterium, spiral in shape. It infects approximately half of the population across the world. Aims: to assess the antibiotic resistance of H. pylori as well as the potential of medical plant extracts to inhibit resistant strains. Materials and Methods: The study was conducted in Microbiology Institute, Shah Abdul Latif University, Khairpur Mir’s from January 2019 to December 2019. Endoscopy was used to obtain samples from the gastrointestinal ward of teaching hospital of KMC, Khairpur. H. pylori was isolated, identified and inoculated. From the local market, ginger roots, garlic roots, kalonji seeds, and mint leaves were acquired and extracts were prepared. RESULTS: Antimicrobial sensitivity of extracts (Garlic, ginger, kalonji and mint) against H. pylori was tested by agar well-diffusion method. All herbal extracts showed more sensitivity extracted in ethanol as compared to distilled water. In contrast mint did not give any results. Ethanol extracts are found to be very effective against H. pylori as compared to distilled water extracts. Conclusion: Garlic, ginger, and kalonji all demonstrated antibacterial property towards H. pylori. Keywords: Helicobacter pylori, Antimicrobial Sensitivity, Plant Extracts, Inhibition Zone

Time-kill kinetics and antimicrobial activities of Thai medical plant extracts against fish pathogenic bacteria.

The main objective of this work was to conduct the microbial control of Thai herbs against fish pathogens and their time-kill kinetics activity. Ten medicinal plants were selected to test antimicrobial activity against aquatic pathogens including Aeromonas hydrophila, Flavobacterium sp., and Streptomyces sp. Caesalpinia sappan and Alpinia galangal extracts showed the best activity against A. hydrophila and Streptomyces sp. Among them, Caesalpinia sappan expressed the great activity against A. hydrophila and Streptomyces sp. with the test concentration of MIC values of 1.25 and 2.50 mg/mL and MBC values of 5.0 and 10.0 mg/mL, while the MIC and MBC values of A. galangal were found to be 2.50 and 10.0 mg/mL with Streptomyces sp. The plant extracts of C. sappan and A. galangal at 1MIC, 2MIC, and 3MIC values really showed time-kill kinetics potential against fish pathogen on period of 3–18 h. In conclusion, plant extracts are good potentials sources as antifish pathogens and safety in an aquatic ecosystem.

Study of antibacterial and antioxidant properties of medical plant extracts

The study analyzed the biological (antimicrobial and antioxidant) activity of such medicinal plants grown in the Kemerovo region as Leaf mustard (Brassica juncea L.), Stinging nettle (Urtica dioica), Maral root (Rhaponticum carthamoides), Thyme (Thymus serpyllum L.), Purple coneflower (Echinacea purpúrea), Chamomile (Chamomilla recutita L.), Milk thistle (Sílybum mariánum L.), Common marigold (Caléndula officinális), Tansy (Tanacétumá vulgáre), and Common dandelion (Tarináxum officinum). It was found that all investigated plant extracts have antimicrobial properties against the tested strains of microorganisms (E. coli ATCC 25922, S. aureus ATCC 25923, P. vulgaris ATCC 63, C. albicans EMTC 34, L. mesenteroides EMTC 1865). It was proved that all tested extracts of medicinal plants are characterized by a high antioxidant status; according to the tests performed this parameter of the samples varies within the limits from 145.09 to 235.00 mg of ascorbic acids per 1 gram. Both the high concentration level of biologically substances and the presence of antimicrobial and antioxidant characteristics of the studied herb extracts from the selected medicinal plants make it possible to recommend their use as components of feed additives for agricultural animals.

Evaluation of Antibacterial Activity between Medical Plant Extracts and Antibiotics against Bacterial Isolates from Tonsillitis

Objectives: Acute tonsillitis is an inflammatory process of tonsil tissue and is usually infectious. Acute tonsillitis starts suddenly and usually disappears within one to two weeks. This study aims to isolate bacteria from patients with infectious tonsillitis and determine the antibacterial activity of Mentha piperita L, Citrus aurantium, banana peel extract and antibiotics including penicillin, ampicillin, oxacillin, meropenem, tobramycin, gentamicin, levofloxacin, and clarithromycin against bacteria.
 Methods: Were Collected 60 clinical samples of sputum and throat swabs from patients with tonsillitis admitted to AL-Furat Alawsait Hospital in Najaf. The samples were cultured in blood, MacConkey agar, and incubated at 37°C for 24 hours, and then the Vitek2 system was used for diagnosis to detect G + ve and G-ve bacteria.
 Results: It was found that out of 60 sputum samples and throat swabs, 15 cases were gram positive bacteria, while 45 gram negative bacteria. As revealed by the in vitro agar well diffusion method, it was found that the aqueous extracts of these plants, especially Mentha piperita, have strong antibacterial activity against a variety of pathogens. The results show that Mentha is very effective at all concentrations used in this study (125, 250, 500, 1000) mg / ml in all bacteria isolated from tonsillitis. The zone of greatest inhibition of Mentha, Citrus anrantium and banana peel extract was 30, 28, 25 mm at concentrations of 1000 mg / ml respectively. Used eight selected antibiotics, including (penicillin, ampicillin, oxacillin, meropenem, tobramycin, gentamicin, levofloxacin, and clarithromycin). To shows the susceptibility of all bacteria isolates used in this study, the best antibiotics to sensitive for all bacteria (Meropenem and Levofloxacin ), while the resistance to oxacillin.
 Conclusion: The selected commonly used aqueous extracts have been tested for their antibacterial activity against multi drug-resistant bacteria, and it is believed that their extracts can be used for multi drug-resistant bacteria that can cause nosocomial and community-acquired infections.

Antischistosomal Activity of Origanum majorana, Ziziphus spina-christi, and Salvia fruticosa Plant Extracts on Hamster Infected with Schistosoma haematobium.

World Health Organization (WHO) has approved only one treatment for schistosomiasis, praziquantel (PZQ), but some poor efficacy was noticed in patients during the early stage of infection. Therefore, researchers have intensified their efforts to research new alternative medicines to treat schistosomiasis. In the present study, in vitro as well as in vivo studies have been accomplished to evaluate the effect of Origanum majorana, Ziziphus spina-christi, and Salvia fruticosa extracts in a different concentration 500, 250, 125, 62.5, and 31.25 μg/ml on golden hamster infected by Egyptian strains of schistosome (Schistosoma haematobium). In vitro, the adult worms and schistosomula of S. haematobium were investigated in RPMI-1640 medium for 48 hrs. The results showed that the concentration 500, 250, and 125 μg/ml of Origanum majorana, and Ziziphus spina-christi caused dead of 100% of Egyptian Schistosoma strains of adult worm and schistosomula of S. haematobium within 6 to 12 hrs of incubation. On the other hand, the extract of Salvia fruticosa at concentrations 500, 250, and 125 μg/ml showed death 100% parasites after 12 to 24 hrs of incubation. Inclusion, Origanum majorana, and Ziziphus spina-christi showed effectiveness against Egyptian Schistosoma strains (S. haematobium), a slight decrease in Salvia fruticosa was observed. Therefore, these medical plant extracts may be used as a safe and effective treatment for schistosomiasis.

From Vietnamese plants to a biflavonoid that relieves inflammation by triggering the lipid mediator class switch to resolution

Chronic inflammation results from excessive pro-inflammatory signaling and the failure to resolve the inflammatory reaction. Lipid mediators orchestrate both the initiation and resolution of inflammation. Switching from pro-inflammatory to pro-resolving lipid mediator biosynthesis is considered as efficient strategy to relieve chronic inflammation, though drug candidates exhibiting such features are unknown. Starting from a library of Vietnamese medical plant extracts, we identified isomers of the biflavanoid 8-methylsocotrin-4′-ol from Dracaena cambodiana, which limit inflammation by targeting 5-lipoxygenase and switching the lipid mediator profile from leukotrienes to specialized pro-resolving mediators (SPM). Elucidation of the absolute configurations of 8-methylsocotrin-4′-ol revealed the 2S,γS-isomer being most active, and molecular docking studies suggest that the compound binds to an allosteric site between the 5-lipoxygenase subdomains. We identified additional subordinate targets within lipid mediator biosynthesis, including microsomal prostaglandin E2 synthase-1. Leukotriene production is efficiently suppressed in activated human neutrophils, macrophages, and blood, while the induction of SPM biosynthesis is restricted to M2 macrophages. The shift from leukotrienes to SPM was also evident in mouse peritonitis in vivo and accompanied by a substantial decrease in immune cell infiltration. In summary, we disclose a promising drug candidate that combines potent 5-lipoxygenase inhibition with the favorable reprogramming of lipid mediator profiles.

In vitro assessment of anti-Trichomonas effects ofZingiber officinale and Lavandula angustifolia alcoholic extracts on Trichomonas gallinae.

Trichomonas gallinae is a parasite that acts as a canker-causing agent and leads to significant loss and mortality, especially in young birds. Metronidazole is the approved drug used for the treatment of trichomoniasis. A non-chemical alternativess such as medical plant extracts are also used to treat this disease due to drug resistance. This study aimed to assess in vitro antitrichomonal effects of Lavandula angustifolia and Zingiber officinale extracts on T. gallinae compared with metronidazole. The T. gallinae samples were obtained from infected pigeons. Multi-well plates filled with different concentrations (5.00, 10.00, 25.00, 50.00, and 100 μg mL-1) were used to perform in vitro analysis. The Z. officinale extract’s minimum inhibitory concentration (MIC) in the 24-hr period was 25.00 μg mL-1, while it was 50.00 μg mL-1 for metronidazole. The MIC value obtained for L. angustifolia extract in 24-hr was 50.00 μg mL-1. The results indicated that the extracts of Z. officinale and L. angustifolia could act as potential natural agents against trichomoniasis. Furthermore, this study delineated the equal efficiency of L. angustifolia and Z. officinale with that of metronidazole in inhibiting the growth of Trichomonas gallinae trophozoites in culture media.

Identification of Withania somnifera-Silybum marianum-Trigonella foenum-graecum Formulation as a Nutritional Supplement to Contrast Muscle Atrophy and Sarcopenia.

Background: Muscle atrophy, i.e., the loss of skeletal muscle mass and function, is an unresolved problem associated with aging (sarcopenia) and several pathological conditions. The imbalance between myofibrillary protein breakdown (especially the adult isoforms of myosin heavy chain, MyHC) and synthesis, and the reduction of muscle regenerative potential are main causes of muscle atrophy. Methods: Starting from one-hundred dried hydroalcoholic extracts of medical plants, we identified those able to contrast the reduction of C2C12 myotube diameter in well-characterized in vitro models mimicking muscle atrophy associated to inflammatory states, glucocorticoid treatment or nutrient deprivation. Based on their ability to rescue type II MyHC (MyHC-II) expression in atrophying conditions, six extracts with different phytochemical profiles were selected, mixed in groups of three, and tested on atrophic myotubes. The molecular mechanism underpinning the effects of the most efficacious formulation, and its efficacy on myotubes obtained from muscle biopsies of young and sarcopenic subjects were also investigated. Results: We identified WST (Withania somnifera, Silybum marianum, Trigonella foenum-graecum) formulation as extremely efficacious in protecting C2C12 myotubes against MyHC-II degradation by stimulating Akt (protein kinase B)-dependent protein synthesis and p38 MAPK (p38 mitogen-activated protein kinase)/myogenin-dependent myoblast differentiation. WST sustains trophism in C2C12 and young myotubes, and rescues the size, developmental MyHC expression and myoblast fusion in sarcopenic myotubes. Conclusion: WST strongly counteracts muscle atrophy associated to different conditions in vitro. The future validation in vivo of our results might lead to the use of WST as a food supplement to sustain muscle mass in diffuse atrophying conditions, and to reverse the age-related functional decline of human muscles, thus improving people quality of life and reducing social and health-care costs.

The Investigation of Antibacterial and Antibiofilm Activity of Some Aqueous Medical Plant Extracts from Iraq Against the Bacteria Isolated from Patients with Thermoplastic Retainer

The Investigation of Antibacterial and Antibiofilm Activity of Some Aqueous Medical Plant Extracts from Iraq Against the Bacteria Isolated from Patients with Thermoplastic Retainer

Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample

In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC). The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase. The HPLC-UV method was used for measuring the extracted RA. The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD). The achieved optimum values for the mentioned variables were 7.0, 10 mg, 4 min, and 180 μL, respectively. The extraction recovery (99.68%) obtained from the predicted model was in agreement with the experimental data (98.22 ± 1.33%). In addition, under the obtained optimum conditions and over the concentration in the range of 2–3500 ng mL−1, a linear calibration curve was obtained with R2 > 0.993. The limit of detection (LOD) and quantification (LOQ) values were computed, and the obtained ranges were respectively from 0.060 to 0.089 ng mL−1 and 0.201 to 0.297 ng mL−1. In addition, the enrichment factors were obtained in the range of 93.7–110.5 with preconcentration factor of 83.3. Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%–105.4% and the precision with relative standard deviation <5.7%.

Needleless electrospun carboxymethyl cellulose/polyethylene oxide mats with medicinal plant extracts for advanced wound care applications

Electrospinning as method for fabrication of wound dressing materials with included medical plant extracts for wound treatment has lately gained increasing attention. However, the transfer of nanofiber fabrication with included plant extracts from the research to the pilot and industrial scale production, using needleless electrospinning is a vital area of research, which could enable its large-scale commercial exploitation. Carboxymethyl cellulose (CMC) is a cheap, water soluble biopolymer, and in blends with the spinning agent polyethylene oxide (PEO) it is a suitable polymer for a large-scale nanofiber production. Thus, this study addresses the needleless electrospinning of CMC/PEO/plant extract blend aqueous solutions in order to fabricate cellulose based wound dressing material, suitable for treatment of acute wounds. The influence of plant extracts on the morphology of the electrospun mats was further evaluated. The antioxidant and antibacterial properties of the as-prepared electrospun mats were determined, where special attention was devoted to the stability/degradation study of phenolic compounds in plant extracts during the electrospinning process. This research was complemented by the release study and cell viability testing with results indicating a promising potential of this product to use for wound care as a self-contained wound dressing or as a part of number of already existing novel wound dressing materials.

Extracts of medical plants suppress the SOS response and reduce mutagenesis in E. coli

One of the promising directions in the fight against the emergence and spread of farm animal microbiota resistance factors is the development and search for feed additives that can inhibit the SOSresponse. SOS-response is one of the main mechanisms of the occurrence of mutations in bacteria. Plants used in traditional medicine can be a promising source of safe substances that reduce the SOS-response of bacteria. A screening of plants potentially containing substances with antiSOS activity was performed. During the initial screening, the E. coli MG 1655 pRecA-lux biosensor strain with ciprofloxacin as RecA inducer was used. Seven plants were identified whose extracts reduced the expression of the RecA operon. In further experiments on bacteria exposed to antibiotics, we identified four plants whose exstracts significantly reduced the mutagenesis rate of clinical E. coli strains: Austrian broom (Cytisus austriacus), greater celandine (Chelidonium majus), walnut (Juglans regia) and smooth sumac (Rhus glabra).

Medical Plants and Nutraceuticals for Amyloid-β Fibrillation Inhibition.

Plaque formation due to amyloid-β oligomerization and fibrillation is a key issue for its deposition in the brains of dementia and Alzheimer’s disease patients. Related drugs preventing this peptide fibril accumulation bear the potential of considerable medical and social value. In this study, we performed in vitro fibrillation inhibition tests with eight different medical plant extracts and nutraceuticals using fluorescence spectroscopy. Successful inhibition of the following plant extracts and nutraceuticals were obtained: Withania somnifera, Centella asiatica, Bacopa monnieri, and Convolvulus pluricaulis, providing new drug candidates for the prevention and treatment of Alzheimer’s disease.

Сhronic toxicity testing complex phytopoliextraction of sedative action

Development and introduction in medical practice sedative meals on the base of complex phytopoliextracts from the domestic medical plants permitted for medical application is highly actual because of incrised incidence of abnormal psychology disorders determined by socioeconomical problems, global informative boom, ecological problems, worsening of life quality.&#x0D; The aim of a study – determination of the sedative phytopoliextraction complex chronic toxicity parameters in experiments after oral administration to laboratory rats.&#x0D; Standardized phytopoliextraction сomplex which is composed of extracts of medical plants (Valeriana officinalis L., Crataégus, Melissa officinalis L., Hypericum, Mentha piperita L., Húmulus lúpulus, Viburnum).&#x0D; It was determined that phytopoliextraction complex is non-toxic compound – LD50 drug after a single oral administration to mice is &gt; 5.0 ml/kg, to rats &gt; 10.0 ml/kg. A two-month administration of a phytopolietextraction complex in an effective and sub-toxic dose (1 ml/kg and 5 ml/kg, respectively) does not exert a toxic effect on the general state of the behavior and the increase in the weight of the animals. Phytopolietextraction complex in the subtotoxic dose does not change the functional state of the CNS in male rats, but in the effective state it increases the research activity. At the same time, it exerts a certain stimulating effect on females, due to the content of ethyl alcohol, and in a sub-toxic dose has a hypoglycemic effect largely due to the presence of ethyl alcohol.&#x0D; Thus, futher experiments have to be provided to learn other chronic toxicity parametres like local localirritating action, embrio- and gonadotoxicity.