Abstract
One of the promising directions in the fight against the emergence and spread of farm animal microbiota resistance factors is the development and search for feed additives that can inhibit the SOSresponse. SOS-response is one of the main mechanisms of the occurrence of mutations in bacteria. Plants used in traditional medicine can be a promising source of safe substances that reduce the SOS-response of bacteria. A screening of plants potentially containing substances with antiSOS activity was performed. During the initial screening, the E. coli MG 1655 pRecA-lux biosensor strain with ciprofloxacin as RecA inducer was used. Seven plants were identified whose extracts reduced the expression of the RecA operon. In further experiments on bacteria exposed to antibiotics, we identified four plants whose exstracts significantly reduced the mutagenesis rate of clinical E. coli strains: Austrian broom (Cytisus austriacus), greater celandine (Chelidonium majus), walnut (Juglans regia) and smooth sumac (Rhus glabra).
Highlights
The use of antibiotic drugs in animal husbandry is a serious problem leading to an increase of bacteria antibiotic resistance.There are two ways in which the use of antibiotics in animals contributes to increase of resistance in humans’ pathogens
To reduce the second pathway of the spread of antibiotic resistance, feed additives should be able to reduce the rate of resistant forms appearance in animal husbandry [1, 2]
C.coggygria, aqueous extract of leaves J.regia, green pericarp extracted with 70% ethanol J.regia, aqueous extract of green pericarp C.austriacus 50% ethanol extract C. ruthenicus 50% ethanol extract
Summary
The use of antibiotic drugs in animal husbandry is a serious problem leading to an increase of bacteria antibiotic resistance.There are two ways in which the use of antibiotics in animals contributes to increase of resistance in humans’ pathogens. To reduce the second pathway of the spread of antibiotic resistance, feed additives should be able to reduce the rate of resistant forms appearance in animal husbandry [1, 2]. One of the promising directions in the fight against the emergence and spread of resistance factors is the development and search for inhibitors of the SOS response, i.e. one of the main mechanisms of the occurrence of mutations in bacteria, which gives them an evolutionary advantage and material for the selection of resistant strains [3]
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