Stabilization Mechanism Research Articles

Insight into the Stabilization Mechanism of Succinylation Modification on Black Bean Protein Gels: Molecular Conformation, Microstructure, and Gel Properties.

The objective of this work was to investigate the effect of succinylation treatment on the physicochemical properties of black bean proteins (BBPI), and the relationship mechanism between BBPI structure and gel properties was further analyzed. The results demonstrated that the covalent formation of higher-molecular-weight complexes with BBPI could be achieved by succinic anhydride (SA). With the addition of SA at 10% (v/v), the acylation of proteins amounted to 92.53 ± 1.10%, at which point there was a minimized particle size of the system (300.90 ± 9.57 nm). Meanwhile, the protein structure was stretched with an irregular curl content of 34.30% and the greatest processable flexibility (0.381 ± 0.004). The dense three-dimensional mesh structure of the hydrogel as revealed by scanning electron microscopy was the fundamental prerequisite for the ability to resist external extrusion. The thermally induced hydrogels of acylated proteins with 10% (v/v) addition of SA showed excellent gel elastic behavior (1.44 ± 0.002 nm) and support capacity. Correlation analysis showed that the hydrogel strength and stability of hydrogels were closely related to the changes in protein conformation. This study provides theoretical guidance for the discovery of flexible proteins and their application in hydrogels.

Role of egg white protein particles’ structure and interface profiles in tailoring high-internal-phase Pickering emulsions with desired applicability

Tailoring food-grade high-internal-phase Pickering emulsions (HIPPEs) with desired applicability has been extremely hindered due to the limited stabilization mechanism deciphering. This study illustrated the specific role of egg white protein (EWP) particlesꞌ structure and interface distribution to the stabilization of HIPPEs. Herein, gelation induced EWP aggregation into its microgel particles (EWPG) with apparent conformation transition (α-helix to β-sheet) and surface activity improvement (e.g., hydrophobicity, interface tension, and wettability). Besides, larger EWPG tended to sparsely absorb on the HIPPEsꞌ interface and achieve admirable long-term stability (6-month storage and 18-days lipid oxidation), owing to higher particle rigidity and steric repulsion. Comparably, EWP (small, soft, and rough) was superior in ready-to-use HIPPEs with centrifugation, freeze-thawing, and nutraceutical delivery demands, accompanied by a stronger rattan-like interfacial network with higher viscoelasticity. Overall, this research would facilitate the facile design of food matrices (e.g., EWP)-based HIPPEs via the gelation-tailored balance of particle structure and interface distribution.

Antifoams in non-aqueous diesel fuels: Thin liquid film dynamics and antifoam mechanisms

HypothesisFoaming in diesel fuels is not well understood and leads to operational challenges. To combat deleterious effects of foaming, diesel formulations can include additives called antifoams. Existing antifoams, unfortunately, are inherently ash-generating when combusted, with unknown environmental impacts. They are prohibited in certain countries, so identifying effective alternative ash-free antifoam chemistries is needed.ExperimentsWe conduct systematic characterization of foam stabilization and antifoaming mechanisms in diesel for two different antifoams (silicone-containing & ashless chemistries). Employing a custom technique combining single-bubble/single-antifoam-droplet manipulation with white light interferometry, we also obtain mechanistic insights into foam stability and antifoam dynamics.ResultsCoalescence times from both bulk foam and single bubble experiments confirm ashless antifoams are effective at reducing foaming, demonstrating the potential of ashless antifoams. Further, we perform single-antifoam-droplet experiments and obtain direct experimental evidence revealing the elusive antifoaming mechanisms. Interestingly, the silicone-containing and ashless antifoams seemingly function via two different mechanisms: spreading and dewetting respectively. This surprising finding refutes conventional wisdom that spreading is likely the only antifoam mechanism in diesels. These results and the reported experimental framework significantly enhance the scientific understanding of non-aqueous foams and will accelerate the engineering of alternative antifoam chemistries for non-aqueous systems.

Whipped cream stabilized by faba bean protein isolate microgel particles substituted for sodium caseinate: Whipping performance and foam stabilization analysis

In this study, faba bean protein isolate microgel particles (FPIMPs) were used as substitutes for sodium caseinate in whipped cream ingredients, the effect of FPIMPs on properties of emulsion system before whipping and foam system after whipping was investigated, and the stabilization mechanisms of whipped cream systems stabilized by FPIMPs and sodium caseinate, respectively, were analyzed and compared. Elevated levels of FPIMPs facilitated the cross-linking and flocculation of oil droplets in the emulsions, which further promoted the formation of gel-like networks, leading to a larger average particle size and higher viscoelasticity. Meanwhile, the firmness, shaping ability, and shape retention ability of whipped creams were gradually enhanced with the increase of FPIMPs content. The quality of whipped creams with FPIMPs concentrations of 0.8% and above was comparable to that of commercial whipped cream. Tribological results showed that the coefficient of friction of whipped creams was roughly negatively correlated with the concentration of FPIMPs, and the lubricating properties of whipped creams with high FPIMPs concentrations (>0.8%) were better than those of commercial whipped cream. The oil-water interface film with high mechanical strength formed by FPIMPs made the FPIMPs-stabilized whipped creams more resistant to whipping than sodium caseinate-stabilized whipped cream, delaying the occurrence of excessive whipping phenomenon. This work has important implications for expanding the application of faba bean protein isolate in food systems, as well as provides new strategies for the development of healthier plant-based whipped creams.

Why are plant communities stable? Disentangling the role of dominance, asynchrony and averaging effect following realistic species loss scenario

Abstract A growing number of studies have demonstrated that biodiversity is a strong and positive predictor of ecosystem temporal stability by simultaneously affecting multiple underlying mechanisms of stability, that is dominance, asynchrony and averaging effects. However, to date, no study has disentangled the relative role of these key mechanisms of stability in biodiversity experiments. We created a species richness gradient by mimicking a loss of rare species and assessed the role of species richness on community stability and, more importantly, quantified the relative role of three stabilizing mechanisms, that is dominance (stabilization due to stable dominants compared to the rest of the species in the community), asynchrony (stabilization due to temporal asynchrony between species), and averaging effects (pure effect of diversity) on community stability across a species richness gradient. We found that extreme species loss negatively impacted community stability, but just three species were enough to stabilize biomass production to a level similar to highly diverse communities. However, the similar stability of communities resulted from differing contributions from each stabilizing mechanism, depending on the community diversity. Since less abundant species were more temporally variable, species loss stabilized the populations of the remaining species. The loss of rare and subordinate species reduced the dominance and averaging effects, but increased the asynchrony effect. Hence, the asynchrony effect played a major role in the stability of species poor communities, while the averaging effect drove most of the stability of species rich communities. Overall, dominance played only a minor role, accounting for 5%–15% of the stabilization, while asynchrony and averaging effects were dominating forces contributing to ~85%–95% of the total stabilization. Synthesis. This study highlights the importance of biodiversity and roles of dominant and rare species for long‐term community stability and, for the first time, disentangles relative roles of dominance effect, asynchrony and averaging effect on community stability in a real‐world biodiversity experiment.

β-Lactoglobulin-based amorphous solid dispersions: A graphical review on the state-of-the-art

Proteins have recently caught attention as potential excipients for amorphous solid dispersions (ASDs) to improve oral bioavailability of poorly water-soluble drugs. Notably, the studies have highlighted whey protein isolates, particularly β-lactoglobulin (BLG), as promising candidates in amorphous stabilization, dissolution and solubility enhancement, achieving drug loadings of 50 wt% and higher. Consequently, investigations into the mechanisms underlying the solid-state stabilization of amorphous drugs and the enhancement of drug solubility in solution have been conducted. This graphical review provides a comprehensive overview of recent findings concerning BLG-based ASDs. Firstly, the dissolution performance of BLG-based ASDs is compared to more traditional polymer-based ASDs. Secondly, the drug loading onto BLG and the resulting amorphous stabilization mechanisms is summarized. Thirdly, interactions between BLG and drug molecules in solution are described as the mechanisms governing the improvement of drug solubility. Lastly, we outline the impact of the spray drying process on the secondary structure of BLG, and the resulting differences in amorphous stabilization and drug dissolution performance between α-helix-rich and β-sheet-rich BLG-based ASDs.

Mechanical instabilities of nano-composite plates using elasticity theories

Mechanical instabilities of nano-composite plates using elasticity theories

High internal phase Pickering emulsions via complexation of cellulose nanofibrils and nanochitin: Enhanced interfacial adsorption and structured aqueous network

High internal phase emulsion is a novel emulsion type for a range of applications, while its preparation is limited by low stability and the selection of proper emulsifiers. Herein, an edible sunflower oil-in-water high internal phase Pickering emulsion (HIPPE) stabilized by complexation of cellulose nanofibrils (CNF) and nanochitin (NCh) is prepared using a two-step method. The stabilization capacity of the CNF/NCh complexes stems from restricted coalescence, droplet breakage, and coarsening upon HIPPE formation, showing the minimal droplet size at 80 μm. This is the result of a dual stabilization mechanism including: enhanced adsorption of the complexes at the oil-water interfaces by existence of the acetyl groups in the complexes and structuring the aqueous phase to form networking structures within HIPPEs. The CNF/NCh complex suspensions exhibit tolerance to changes in pH (3–5) and salinity (0–500 mM), showing capability of forming and stabilizing HIPPEs with minimal alteration to droplet size and morphology. The complex-stabilized HIPPEs remain stability after at least 2-year storage at ambient condition. Overall, complexation of CNF and NCh greatly extend the conditions on forming highly stable, tuneable HIPPEs, offering potential on engineering applications relevant to clean-label, green foodstuffs.

The interactions between zeolite and two cellulose derivatives: A comprehensive analysis of liquid and solid phase properties

This study investigates the impact of cellulose-derived polymers, anionic carboxymethylcellulose (CMC), and cationic cellulose (CC) on the colloidal and thermal stability of zeolite Na-X materials. By exploring polymer adsorption onto Na-X surfaces and characterising the resultant materials, using FT-IR, XPS, SEM, PSD, CHN, and zeta potential, the research unveils how CMC and CC modify zeolite properties. This investigation elucidates the potential roles of these polymers in colloidal systems with zeolites, revealing their promise for crafting organic-inorganic materials. Additional insight was also provided by careful examination of the thermal stability (TGA-DSC) of the obtained cellulose/zeolite materials. Furthermore, the study distinguishes the different adsorption mechanisms of CMC and CC, with CMC relying on some weak interactions (H-bonding and van der Waals forces), while CC interacts mainly via electrostatic forces. Both CMC and CC can act as stabilizing agents, with CMC being more efficient and using both electrosteric and depletion stabilizations. Importantly, the concentration of CC plays a role in bridging flocculation, highlighting the concentration-dependent nature of the stabilization mechanism.

A microfluidic and Langmuir approach to unveil the interplay between hydroxypropyl methylcellulose and zein nanoparticles on the stability of Pickering emulsions

Utilizing microfluidic technology and Langmuir trough, the study investigated how hydroxypropyl methylcellulose (HPMC) interact with zein nanoparticles (ZNPs) at interfaces and their collective impact on emulsion stability. Herein, microchips were designed with varying adsorption channel lengths to study the effect of adsorption kinetics on the coalescence stability, while Langmuir assessments provided insights into particle adsorption kinetic and film properties of emulsion stabilization mechanisms. Results suggested that HPMC's incorporation expedited ZNPs' rearrangement rates at the interface, especially at low concentrations, resulting in a synergistic reduction in interfacial tension. However, at high HPMC concentration, a competitive adsorption scenario emerged between ZNPs and HPMC, leading to increased interfacial tension and instability. Microfluidic analyses of droplet coalescence, alongside Langmuir-derived interfacial rheology data, revealed that while a small amount of HPMC significantly bolstered emulsion stability by enhancing viscoelastic properties of interfacial film, an excess of HPMC inversely affected these parameters, diminishing film elasticity and thus, the emulsion's coalescence resistance. The adsorption kinetic and the interaction on interface of HPMC and zein particles were analyzed via microfluidic and LB technology in this study, which will not only expand our current strategies for interface engineering of emulsions, but also deepens our understanding of the interplay between mixed particle system adsorption properties, interfacial rheology, and Pickering composite emulsion stability.

Differential physical interactions of wild-type and S59L mutant CHCHD10 with mitochondrial proteases

Background CHCHD10 is a small mitochondrial protein with a C-terminal coiled-coil-helix–coiled-coil-helix domain. The N-terminal region of CHCHD10 is mostly intrinsically disordered. Therefore, CHCHD10 has no catalytic activity other than protein-protein interactions through the CHCH domain or intrinsically disordered region. The S59L mutation in CHCHD10 has been identified as a genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia. However, the disease-causing mechanisms of CHCHD10S59L are not yet fully understood. Our previous study showed that CHCHD10S59L induces PINK1 and Parkin accumulation. PINK1 stabilization in the mitochondria is dependent on proteolytic processing by mitochondrial proteases. It has also been reported that mutant CHCHD10 disrupts protein homeostasis within mitochondria. Therefore, we hypothesized that CHCHD10S59L physically interacts with mitochondrial proteases during the disease pathogenesis. Methods To test this hypothesis, we performed co-immunoprecipitation using transiently transfected wild-type and mutant S59L CHCHD10 in HeLa cells. We employed a dithiobis (succinimidyl propionate) cross-linker to detect transient physical interactions. Results We expected that CHCHD10S59L may activate mitochondrial protease processing or expression and that it interacts with more diverse mitochondrial proteases than the wild type. In general, however, there was no difference in the expression and processing levels of mitochondrial proteases and the proteases that interact with wild-type and CHCHD10S59L. LonP1 degrading unfolded proteins in the mitochondrial matrix was the major proteases that can bind to wild-type CHCHD10 and CHCHD10S59L. LonP1 and HtrA2 were significantly less strongly bound to CHCHD10S59L. Conclusions This result can be interpreted in many different ways, including the regulatory role of CHCHD10 in mitochondrial proteases. However, more precise biochemical and cell biological investigations are required to determine the role of CHCHD10 in the activity of mitochondrial proteases, mechanism of PINK1 stabilization, and disruption of mitochondrial proteostasis.

Modular invariant hilltop inflation

In this paper we show that it is possible to achieve successful hilltop inflation in which the inflaton is identified as the modulus field in a modular invariant theory.The dilaton plays a crucial role in shaping the potential.Modular invariant gaugino condensation provides the mechanism for the modulus stabilisation after inflation. The inflationary trajectory lies on the lower boundary of the fundamental domain of the modulus field τ. Inflation starts near the fixed point τ = i, and ends at a point near τ = ω, which is the global de Sitter vacuum.We investigate the allowed parameter space for successful modular invariant hilltop inflation.

Transformation of As and Cd associated with Fe-Mn-modified biochar during simultaneous remediation on the contaminated soil.

Here, Fe- and Mn-modified biochar (BC-Fe-Mn) was applied to simultaneously stabilize As and Cd in the contaminated soil. The removal efficiencies for NaHCO3-extractable As and DTPA-extractable Cd by BC-Fe-Mn were 60.8% and 49.6%, respectively. The speciation analyses showed that the transformation to low-crystallinity Fe-bound (F3) As, Fe-Mn oxide-bound (OX) of Cd, and residual As and Cd was primarily attributed to stabilizing the two metal(loid)s. Moreover, the correlation analyses showed that the increase of As in F3 fraction was significantly and positively associated with the increase of OX fraction Mn (r = 0.64). Similarly, OX fraction Cd was increased notably with increasing OX fraction Fe (r = 0.91) and OX fraction Mn (r = 0.76). In addition, a novel dialysis experiment was performed to separate the reacted BC-Fe-Mn from the soil for intensively investigating the stabilization mechanisms for As and Cd by BC-Fe-Mn. The characteristic crystalline compounds of (Fe0.67Mn0.33)OOH and Fe2O3 on the surface of BC-Fe-Mn were revealed by SEM-EDS and XRD. And FTIR analyses showed that α-FeOOH, R-COOFe/Mn+, and O-H on BC-Fe-Mn potentially served as the reaction sites for As and Cd. A crystalline compound of MnAsO4 was found in the soil treated by BC-Fe-Mn in the dialysis experiment. Thus, our results are beneficial to deeper understand the mechanisms of simultaneous stabilization of As and Cd by BC-Fe-Mn in soil and support the application of the materials on a large scale.

Potential of Marine Macroalgae in Stabilizing Bio-Fabricated Nanoparticles for Photodegradation Application: A Review

Marine macroalgae, or seaweeds, have emerged as promising sources for the green synthesis and stabilization of nanoparticles, offering remarkable potential in photodegradation processes. These abundant and renewable resources harbor a diverse array of bioactive compounds, including polysaccharides, proteins, flavonoid, and pigments, which can act as reducing, capping, and stabilizing agents. The integration of macroalgae-derived stabilizing agents into nanoparticle synthesis confers unique properties, enhancing photocatalytic performance and stability. This review comprehensively examines the role of marine macroalgae as stabilizing agents for bio-fabricated nanoparticles in photodegradation applications in particular for toxic azo dye degradation and organic compounds. It explores into the mechanisms of nanoparticle synthesis and stabilization, explores the diverse macroalgal species employed, and evaluates their photocatalytic efficiency against various organic and inorganic pollutants in wastewater. Furthermore, the review critically analyzes the challenges and future perspectives in this field, offering insights into the development of green, sustainable, and efficient remediation strategies for environmental contaminants.

Analysis of changes in shale mechanical properties and fault instability activation caused by drilling fluid invasion into formations

During the drilling process, the issue of drilling fluid loss can lead to changes in the mechanical properties of the formation, thereby altering the stress environment of nearby faults. In order to assess the risk of fault activation during drilling operations, the Ordos M area shale was selected as the research object. Mechanical experiments were conducted on rock samples immersed in water-based drilling fluid with a pressure differential of 2 MPa and a temperature of 50 °C. The changes in the mechanical properties of the shale before and after immersion in drilling fluid were determined. Based on the experimental results, combined with the spring combination model and fault activation theory, a quantitative evaluation of fault activation risk was conducted. The findings revealed that the shale in this region has a high clay content, demonstrating a certain level of water sensitivity. The presence of micro-pores and micro-fractures is well-developed, increasing the interaction probability between drilling fluids and clay minerals. After immersion in drilling fluid, there was a varied decline in all mechanical strengths of the shale. The elastic modulus is positively correlated with the shear strength and Coulomb stress of the fault plane. The Poisson’s ratio is positively correlated with the shear strength and negatively correlated with the Coulomb stress. The greater the internal friction and cohesion, the higher the shear strength of the fault plane, and the larger the friction coefficient, the smaller the Coulomb stress, resulting in a more stable fault.

Trendelenburg gait after total hip arthroplasty due to reduced muscle contraction of the hip abductors and extensors

BackgroundDespite experiencing pain relief and improved activities of daily living after total hip arthroplasty (THA) for osteoarthritis of the hip, a Trendelenburg gait may be observed in some patients. The concentric and eccentric contraction patterns of hip muscles in a Trendelenburg gait are not well understood. MethodsThis study included 89 patients (28 males and 61 females, mean age 66.5 ± 8.4 years, mean postoperative period 1.3 ± 0.4 years) after unilateral THA without functional impairment on the contralateral side. Gait analysis utilized a three-dimensional motion capture system to assess pelvis and hip angles, hip moment, and hip power. A Trendelenburg gait was defined as positive when nonoperative pelvic descent occurred at 30 % of the gait cycle, equivalent to mid-stance. Patients were classified into Trendelenburg gait-positive and -negative groups for statistical analysis. Unpaired t-test and chi-square test were used to compare the two groups. Multiple regression analysis was conducted to identify factors associated with the presence of a Trendelenburg gait. ResultsA Trendelenburg gait was observed in 24 patients (27 %). Multiple regression analysis indicated that abduction (p < 0.01) and extension (p = 0.03) of hip joint power were significant determining of a Trendelenburg gait. Patients with a Trendelenburg gait exhibited reduced eccentric contraction of the hip abductor muscles and decreased concentric contraction of hip extensor muscles during early to mid-stance of their gait. ConclusionCentrifugal contraction of hip abductor muscles and diminished eccentric contractility of hip extensor muscles appear crucial for hip stabilization mechanisms during gait after THA.

Pectin-based cinnamon essential oil Pickering emulsion film with two-sided differential wettability: A major role in the spatial distribution of microdroplets

Pickering emulsions have attracted much attention as a novel emulsifying technology. This research to explore Zein-Citrus pectin nanoparticles stabilized cinnamon essential oil (CEO) Pickering emulsion (ZCCPEs) for constructing Pickering emulsion edible film (PEF). Unlike traditional research, which focuses on antibacterial and antioxidant activities, our research examined the physical properties of PEF, specifically changes in wettability. The results show that PEF has better transparency and tensile strength than the pectin alone direct emulsion film (PAEF), and the spatial distribution of Pickering emulsion droplets gives different wettability on both sides of PEF. The partially hydrophobic upside has important application value in food packaging. At the same time, the PEF is biodegradable and environmentally non-polluting. The edible film loaded with essential oils, developed based on the Pickering stabilization mechanism in this study, possesses several desirable characteristics for potential used as bioactive packaging films in food applications.

Metallic biomaterials in biopharmaceuticals and biomedical applications

Metallic biomaterials have captured a lot of interest because of their distinct mechanical characteristics, biocompatibility, and usefulness in biopharmaceutical and biomedical applications. These materials are essential for the creation of several medical equipment and treatment strategies. They include titanium, cobalt-chromium alloys, stainless steel, and biodegradable metals. Metallic implants in orthopaedics offer strong support for bone mending and joint replacement; they have outstanding load-bearing capacity and are resistant to corrosion and wear. Metallic heart valves and stents provide structural integrity and functionality in cardiovascular applications, enhancing patient outcomes in heart valve and coronary artery illness. Metallic biomaterials are manufactured systems created to give biological tissues intrinsic support, and they are commonly employed in stents, dental implants, orthopaedic fixations, and joint replacements. Increased implant-related issues are linked to higher biomaterial utilization because of weak implant integration, infections, mechanical instability, necrosis, and inflammation, as well as ensuring extended patient care, discomfort, and functional loss. The performance and integration of metallic biomaterials inside biological systems have been improved by developments in surface modification techniques, such as coating with biocompatible polymers and drug-eluting technology. Researchers are exploring the possibility of using biodegradable metals that eventually dissolve within the body reducing the risk of long-term issues and repeat procedures. Metallic nanoparticles have also demonstrated potential in increasing therapeutic efficacy, minimizing systemic adverse effects, and selectively targeting disease locations when included in drug delivery systems. The main metallic biomaterials will be briefly discussed in this review, along with the most important established and new methods for modification, which are used to enhance the durability, flexibility, biointegration, and of the biometals, and boost their suitability for 3D printing.

Advancements in Nanomaterial Dispersion and Stability and Thermophysical Properties of Nano-Enhanced Phase Change Materials for Biomedical Applications.

Phase change materials (PCMs) are materials that exhibit thermal response characteristics, allowing them to be utilized in the biological field for precise and controllable temperature regulation. Due to considerations of biosafety and the spatial limitations within human tissue, the amount of PCMs used in medical applications is relatively small. Therefore, researchers often augment PCMs with various materials to enhance their performance and increase their practical value. The dispersion of nanoparticles to modify the thermophysical properties of PCMs has emerged as a mature concept. This paper aims to elucidate the role of nanomaterials in addressing deficiencies and enhancing the performance of PCMs. Specifically, it discusses the dispersion methods and stabilization mechanisms of nanoparticles within PCMs, as well as their effects on thermophysical properties such as thermal conductivity, latent heat, and specific heat capacity. Furthermore, it explores how various nano-additives contribute to improved thermal conductivity and the mechanisms underlying enhanced latent heat and specific heat. Additionally, the potential applications of PCMs in biomedical fields are proposed. Finally, this paper provides a comprehensive analysis and offers suggestions for future research to maximize the utilization of nanomaterials in enhancing the thermophysical properties of PCMs for biomedical applications.

Antimicrobial potential of oregano essential oil vehiculated in Pickering cellulose nanofibers-stabilized emulsions

Essential oils show several biological properties, such as antimicrobial activity, but have limitations regarding their availability and stability. To maximize their antimicrobial effect and protection against environmental conditions, Pickering-type emulsions were used to vehiculate oregano essential oil (OEO) using cellulose nanofibers (CNF) as emulsion stabilizer. Enzymatic hydrolysis was used to produce CNF from a food industry waste (cassava peel), obtaining an environmentally sustainable emulsion stabilizer. It was evaluated how the different properties of the nanofibers affected the stability of the emulsions. Furthermore, the composition of the dispersed phase was varied (different ratios of OEO and sunflower oil-SO) in view of the target application in biodegradable active coatings. Even at very low concentration (0.01 % w/w), CNF was able to form kinetically stable emulsions with small droplet sizes using oil mixtures (OEO + SO). The stabilization mechanism was not purely Pickering, as there was a reduction in interfacial tension. Excellent antimicrobial activity was observed against bacteria and the fungus Alternaria alternata, demonstrating the ability to apply these emulsions in active systems such as coatings and films. An improvement in the stability of emulsions was observed when using a mixture of oils, which is extremely advantageous considering costs and stability to heat treatments, since the desired antimicrobial activity is maintained for the final application.