Mechanism Of Contraction Research Articles

Whole Exome Sequencing Reveals Candidate Variants in Ion Channel Genes for Pelvic Muscle Dysfunction in Young Females with a Family History.

Pelvic floor dysfunction usually results in pelvic organ prolapse (POP) and/or urinary incontinence. In women, several factors, including pregnancy and vaginal delivery, can affect pelvic muscle conditions. The aim of the study was to perform a genetic analysis in young women with a family history of pelvic floor dysfunction to find potentially harmful variants or variants that increase the risk of developing pelvic floor disorders. We employed whole exome sequencing to test ten young women with pelvic floor muscle dysfunction (along with their parents) and a family history. The average age of symptoms was 29.1 (± 3.98) years old, soon after their first delivery. In five out of ten patients, trio-based WES analysis revealed potentially pathogenic, causative nonsense variants in ion channel genes, including ATP1A4, CLCN1, GRIN2C, and ORAI1, as well as missense variants in PIEZO1 and RYR1. Additionally, some of these patients had variants in genes related to muscle function (MUSK) and connective tissue disorder (FKBP14, p.Glu122ArgfsTer7). The variants found in this study, such as CLCN1 (p.Arg894Ter) and MUSK (p.Val790Met), have already been associated with neuromuscular channelopathy and severe muscle weakness. The identified candidate genes encode mainly proteins involved in electrical action potential and mechanical muscle contraction. The results suggest that the identified genetic variants may result in skeletal muscle ion channelopathies that affect muscle function, gradually leading to muscle hypotonia and weakness.

A Review on Porous Structure Negative Poisson's Ratio Metamaterials

Metamaterials with a porous structure possess a number of advantageous properties, including low mass, high specific strength, and a high energy absorption rate. When subjected to impact or compression, these materials exhibit excellent mechanical properties, which has led to their use in a variety of aerospace applications in recent years. In comparison with traditional porous materials, metamaterials possess mechanical and physical properties that traditional materials lack. This distinguishes them from traditional materials and provides new ideas for solving engineering problems. Starting from this deformation mechanism of concave contraction of such structures, this paper details the modeling of several types of porous negative Poisson's ratio superstructures, such as honeycomb, foam and tubular structures, and focuses on the mechanical properties of porous structures in terms of elasticity, impact resistance, and energy absorption when subjected to external forces. The paper commences with an exposition of the mechanism of negative Poisson's ratio effect of porous structure, accompanied by a discourse on the extant research status of several prevalent types of porous negative Poisson's ratio superstructures. Thereafter, the deformation mechanism of porous negative Poisson's ratio metamaterials under compressive load and impact is examined. conditions is introduced, and finally, the practical application of porous negative Poisson's ratio metamaterials in the fields of automobile industry, shipbuilding and other engineering fields are summarized, and the future of porous negative Poisson's ratio metamaterials is reviewed based on the existing research results. Finally, the practical applications of negative Poisson's ratio metamaterials with porous structure in engineering fields such as automobile industry and shipbuilding are summarized, and the future development trend and application prospects are prospected based on the existing research results.

The C. elegans uv1 neuroendocrine cells provide mechanosensory feedback of vulval opening.

Neuroendocrine cells react to physical, chemical, and synaptic signals originating from tissues and the nervous system, releasing hormones that regulate various body functions beyond the synapse. Neuroendocrine cells are often embedded in complex tissues making direct tests of their activation mechanisms and signaling effects difficult to study. In the nematode worm C. elegans, four uterine-vulval (uv1) neuroendocrine cells sit above the vulval canal next to the egg-laying circuit, releasing tyramine and neuropeptides that feedback to inhibit egg laying. We have previously shown uv1 cells are mechanically deformed during egg laying, driving uv1 Ca2+ transients. However, whether egg-laying circuit activity, vulval opening, and/or egg release triggered uv1 Ca2+ activity was unclear. Here we show uv1 responds directly to mechanical activation. Optogenetic vulval muscle stimulation triggers uv1 Ca2+ activity following muscle contraction even in sterile animals. Direct mechanical prodding with a glass probe placed against the worm cuticle triggers robust uv1 Ca2+ activity similar to that seen during egg laying. Direct mechanical activation of uv1 cells does not require other cells in the egg-laying circuit, synaptic or peptidergic neurotransmission, or TRPV and Piezo channels. EGL-19 L-type Ca2+ channels, but not P/Q/N-type or Ryanodine Receptor Ca2+ channels, promote uv1 Ca2+ activity following mechanical activation. L-type channels also facilitate the coordinated activation of uv1 cells across the vulva, suggesting mechanical stimulation of one uv1 cells cross-activates the other. Our findings show how neuroendocrine cells like uv1 report on the mechanics of tissue deformation and muscle contraction, facilitating feedback to local circuits to coordinate behavior.Significance Statement Neuroendocrine cells respond to diverse physical and chemical signals from the body, releasing hormones that control reproduction, gut motility, and fight or flight responses. Neuroendocrine cells are often found embedded in complex tissues, complicating studies of how they are activated. Using the genetic and experimental accessibility of C. elegans, we find the uv1 neuroendocrine cells of the egg-laying motor behavior circuit respond directly to mechanical stimulation and vulval opening. We show that L-type voltage-gated Ca2+ channels facilitate uv1 mechanical activation and coordinate cell activation across the vulval opening. In contrast to other mechanically activated cells, uv1 activation does not require Piezo or TRPV channels. This work shows how neuroendocrine cells relay critical mechanosensory feedback to circuits that control reproduction.

Decision-making in cross-border e-commerce supply chains and coordination under revenue sharing and deferred payment contracts

Deferred payment and revenue-sharing contracts are significantly important for promoting the collaboration and the management of retail export supply chains for cross-border e-commerce. This research addresses the real-world challenges faced by managers in this domain by using a joint optimization model to investigate the best ordering and pricing tactics within cross-border e-commerce retail export supply chains, particularly taking into account export tax rebates and import tariffs. Our findings reveal that while revenue-sharing contracts and deferred payment mechanisms can significantly enhance supply chain profitability, their effectiveness is contingent on variables such as export rebate rates, tariffs, and tariff transfer factors. The practical implications of this study suggest that business administrators should carefully assess these factors when designing contracts to ensure robust supply chain coordination. When traditional contract mechanisms fail, hybrid approaches combining revenue-sharing and deferred payment can offer superior outcomes, thus providing a strategic advantage in volatile markets. These insights are crucial for managers seeking to navigate the complexities of international trade and optimize their supply chain performance.

Impact of magnetorheological fluid composition on their behaviour in gradient pinch mode

Magnetorheological (MR) fluids can be utilized in one of the fundamental operating modes of which the gradient pinch mode has been the least explored. In this unique mode non-uniform magnetic field distributions are taken advantage of to develop a so-called Venturi-like contraction in MR fluids. By adequately directing magnetic flux the material can be made solidified in the regions near the flow channel wall, thus creating a passage in the middle of the channel for the fluid to pass through. This leads to unique variations of the slope in the pressure-flow rate characteristics. It can be stated that the effect of the MR fluid composition on the behaviour of the MR fluid in gradient pinch mode has not been thoroughly investigated yet. In this study, the behaviour of MR fluids was assessed with a dedicated pinch mode MR valve that provided a valuable insight into the contraction mechanism using fluorescence microscopy. Briefly, seven MR fluid samples were prepared with different particle concentrations (10%, 22% and 32 vol%) and mean particle sizes (2, 4.5 and 8.2 μm). It was found that the MR fluid sample with the larger particle size exhibits a significantly larger slope change observed in the pressure-flow rate characteristics. Increasing the particle size from 2 μm (base) to 8 μm resulted in the slope increase by a factor of 2.6 compared to the base sample. Increasing the particle concentration has a negligible effect on the pinch mode effect. Finally, these results were analysed using the modified Wuest equation, which is commonly used for characterizing sharp-edged orifices in low Reynolds number flow regimes. The simple equation was determined to describe the behaviour of MR fluids in gradient pinch mode with adequate accuracy.

Violation of Exclusive Distribution Agreement: Case Study of PT Unilever Indonesia Tbk vs PT Indomarco Prismatama

Breach of exclusive distribution agreement is one of the critical issues that often arise in business relationships between manufacturers and distributors, especially in competitive industries such as fast-moving consumer goods. The case involving PT Unilever Indonesia Tbk and PT Indomarco Prismatama (Indomaret) is a clear example of such a dispute, where the dispute arose from an alleged breach of an exclusive distribution agreement by Indomaret. Unilever claimed that Indomaret, acting as one of their main distributors, had sold Unilever's products outside the terms stipulated in the exclusive agreement, including selling the products below the agreed price or even to third parties not included in the authorized distribution network. This study examines in depth the legal aspects of exclusive distribution agreements in the context of business disputes in Indonesia, with a focus on the Supreme Court's decision number 1559 K/Pdt/2015 which upheld Unilever's claim for breach of contract by Indomaret. Through normative juridical analysis, this research seeks to explore the implications of the ruling for product distribution practices in Indonesia, as well as how such breaches may affect the long-term business relationship between manufacturers and distributors. The analysis shows that exclusive distribution agreements are contractual instruments that have a significant impact on competition in the market. When one party breaches the agreed terms, whether in terms of price, distribution area, or other requirements, the impact is not only limited to legal aspects, but also touches on aspects of reputation and business relationships. In this case, the Supreme Court ruled that PT Indomarco Prismatama was guilty of breach of contract and was required to compensate PT Unilever Indonesia Tbk. This judgment emphasizes the importance of compliance with exclusive distribution contracts and the need for strict sanctions to maintain market integrity. The broader impact of this breach also includes aspects of fair competition. This research highlights how non-compliant distribution practices can affect market dynamics, especially when large distributors such as Indomaret are involved. Under these conditions, exclusive distribution violations can create unfair conditions for other business actors who operate in accordance with existing rules. Therefore, in addition to the legal implications of breach of contract, this study also proposes stricter regulations and effective monitoring mechanisms to prevent such practices from occurring in the future. In addition, this study also provides insights into how the business relationship between producers and distributors should be maintained through a clear and transparent contractual mechanism. One of the key findings of this research is the importance of detailed agreements and strict sanctions against violations, as well as the need for close supervision of contract implementation, especially in highly competitive industries. This applies not only to the consumer goods sector, but also to other sectors that rely on extensive and complex distribution systems. Thus, this research contributes to the understanding of the importance of exclusive distribution agreements in maintaining a balance between the business interests of producers and distributors, as well as in ensuring the creation of a competitive and fair business climate. This research also enriches the literature on business and distribution law in Indonesia, and provides practical insights for companies involved in distribution agreements to avoid potential disputes in the future.

ALPK2 prevents cardiac diastolic dysfunction in heart failure with preserved ejection fraction.

Protein phosphorylation, controlled by protein kinases, is central to regulating various pathophysiological processes, including cardiac systolic function. The dysregulation of protein kinase activity plays a significant role in the pathogenesis of cardiac systolic dysfunction. While cardiac contraction mechanisms are well documented, the mechanisms underlying cardiac diastole remain elusive. This gap persists owing to the historical focus on systolic dysfunction in heart failure research. Recently, heart failure with preserved ejection fraction (HFpEF), an age-related disease characterized by cardiac diastolic dysfunction, has emerged as a major public health concern. However, its underlying mechanism remains unclear. In this study, we investigated cardiac protein kinases by analyzing the gene expression of 518 protein kinases in human tissues. We identified alpha-kinase 2 (ALPK2) as a novel cardiac-specific atypical kinase and generated tamoxifen-inducible, cardiomyocyte-specific Alpk2-knockout mice and Alpk2-overexpressing mice. Alpk2 deficiency did not affect cardiac systolic dysfunction in the myocardial infarction model or the pressure-overload-induced heart failure model. Notably, cardiomyocyte-specific Alpk2 deficiency exacerbated cardiac diastolic dysfunction induced by aging and in the HFpEF model. Conversely, Alpk2 overexpression increased the phosphorylation of tropomyosin 1, a major regulator that binds myosin to actin, and mitigated cardiac stiffness in HFpEF. This study provides novel evidence that ALPK2 represents a potential therapeutic target for cardiac diastolic dysfunction in HFpEF and age-related cardiac impairments.

Abstract 4139162: Optical coherence tomography measures mechanical contractile properties of the beating murine heart

Introduction: Optical imaging techniques can characterize properties of tissue at high temporal and spatial resolution, offering unique insights into electrically excitable tissues such as nerve and muscle. We hypothesized that the gated high-speed optical coherence tomography (OCT) could profile the contractile properties of mouse myocardial tissue and facilitate investigations of microscale mechanical function in the beating heart. Methods: C57BL/6 male mice (n=6) underwent a sternotomy while anesthetized and ventilated and were imaged at baseline with OCT. Another cohort of C57BL/6 male mice (n=6) were sacrificed and the hearts were ex vivo perfused with crystalloid using a Langendorff system. These hearts were imaged at baseline and after inhibition of contractile function with blebbistatin. OCT was performed using a custom tissue stabilizer and a Thorlabs TEL 321 OCT system, which was synchronized to physiologic signals using custom hardware and software and cardiac pacing protocols (Fig. 1A and 1B). OCT cross-sectional B-scan images (Fig. 1C) were obtained throughout multiple cardiac cycles in both in vivo and ex vivo preparations. Data were processed in MATLAB and ImageJ using the workflow in Fig. 1D. Results: Highly reproducible cross-sectional mean intensity changes were observed over the contractile cycle in vivo and ex vivo (Fig. 1E, left and middle). With mechanical contraction inhibited by blebbistatin, this cyclical intensity extinguished, linking the optical scattering change measured by OCT to myocardial contraction (Fig. 1E, right). B-scan curves averaged over multiple mice and temporally aligned with the ECG waveform (Fig. 1F) demonstrate a signal intensity waveform that reproducibly varies with the contractile cycle for both in vivo and ex vivo preparations. In vivo and ex vivo baseline waveforms both had peak intensity during systole and minimum in end-diastole (Fig. 1F). Reproducibility in ex vivo beating heart preparations without blood perfusion provides evidence that myocardial mechanical properties, not red blood cell flow, generate the intensity changes. Conclusion: Gated high-speed OCT imaging can quantify the mechanical contraction wave in the myocardium. Combined with gated OCT angiography, OCT techniques will provide high resolution imaging of both blood flow and mechanical contractility of the beating heart in small animal models of heart disease.

The Relationship Between the Psychological Contract and Job Satisfaction of Beginning College Student Employees

In the context of the rapidly evolving economic landscape, the relationship between the psychological contracts of newly-graduated entry-level employees and their job satisfaction has emerged as a pivotal research topic within the realm of organizational management. The psychological contract, an informal and subjective promise of employment relations, directly influences employees' attitudes and performance. Various types of psychological contracts, such as transactional, relational, and developmental, to some extent, impact employees' job satisfaction. By effectively managing psychological contracts, enterprises can enhance employees' job satisfaction, thereby achieving a win-win situation. This article elucidates the impact mechanism of psychological contracts on job satisfaction and proposes a series of measures to improve the job satisfaction of newly-graduated entry-level employees. These measures include establishing a robust psychological contract, providing systematic introductory training, fostering a supportive work environment, implementing fair performance management, and promoting teamwork and social interaction.

High-resolution chromosome-level genome of Scylla paramamosain provides molecular insights into adaptive evolution in crabs

BackgroundEvolutionary adaptation drives organismal adjustments to environmental pressures, exemplified in the diverse morphological and ecological adaptations seen in Decapoda crustaceans, particularly brachyuran crabs. Crabs thrive in diverse ecosystems, from coral reefs to hydrothermal vents and terrestrial habitats. Despite their ecological importance, the genetic mechanisms underpinning their developmental processes, reproductive strategies, and nutrient acquisition remain poorly understood.ResultsHere, we report a comprehensive genomic analysis of the green mud crab Scylla paramamosain using ultralong sequencing technologies, achieving a high-quality chromosome-level assembly. The refined 1.21 Gb genome, with an impressive contig N50 of 11.45 Mb, offers a valuable genomic resource. The genome exhibits 33,662 protein-coding genes, enriched in various pathways related to development and environmental adaptation. Gene family analysis shows expansion in development-related pathways and contraction in metabolic pathways, indicating niche adaptations. Notably, investigation into Hox gene regulation sheds light on their role in pleopod development, with the Abd-A gene identified as a linchpin. Post-transcriptional regulation involving novel-miR1317 negatively regulates Abd-A levels. Furthermore, the potential role of fru gene in ovarian development and the identification of novel-miR35 as a regulator of Spfru2 add complexity to gene regulatory networks. Comparative functional analysis across Decapoda species reveals neo-functionalization of the elovl6 gene in the synthesis of long-chain polyunsaturated fatty acids (LC-PUFA), suggesting its importance in environmental adaptation.ConclusionsOur findings shed light on various aspects of crab biology, including genome sequencing, assembly, and annotation, as well as gene family expansion, contraction, and regulatory mechanisms governing crucial developmental processes such as metamorphosis, reproductive strategies, and fatty acid metabolism.

Precursory crater contraction associated with the 2017 eruption of Shinmoe-dake volcano (Japan) detected by PALSAR-2 and Sentinel-1 InSAR

The time series of PALSAR-2 and Sentinel-1 images reveal inflation at the volcanic flank and contraction at the crater for approximately 5 months before the 2017 eruption of Shinmoe-dake volcano, Japan. While the observation of inflation at the volcano’s flank is ubiquitous, few studies have reported crater contraction preceding an eruption. The flank inflation stopped after the 2017 eruption, while the contraction at the crater continued until the 2018 eruption. We found that a pipe-shaped deformation source above sea level best fits the observation preceding the 2017 eruption. Suppose the flux of ejected materials constrains the conduit radius during the previous 2011 eruption. In that case, the amount of deformation of the pipe-shaped deformation source, whether open or closed at its top, is too large to be realistic. Although constraining the conduit radius from the eruption flux overestimates the pressure change of the pipe-shaped deformation source, water-saturated fractures along the volcanic conduit could extend the effective conduit radius of the pressure source. We propose one potential scenario for the mechanism of the crater contraction preceding volcanic eruptions based on the combination of compaction due to cooling by ambient groundwater and material withdrawal within the conduit. The groundwater inflows from the ambient aquifer through cracks in the porous conduit wall, which are generated by conduit expansion during the magma ascent. Decoupling from the conduit wall due to a decrease in volume of the material promotes material instability and crater contraction. The interaction between the groundwater and the magma triggers the 2017 eruption of Shinmoe-dake volcano, as previous studies have reported.Graphical

From Fragmented Facts to Unified Knowledge: Exploring Concept Mapping in Neuromuscular Physiology Among First-Year Medical Students.

Background First-year medical students may find it challenging to integrate complex physiological concepts, particularly neuromuscular physiology. While concept mapping has shown promise in medical education, its specific application in teaching intricate physiological mechanisms still needs to be explored. With this background, the objective of the study was toassess the feasibilityof using concept mapping among first-year medical students and to explore the perception of students about concept mapping as an educational tool. Methods A mixed-methods study was conducted with first-year medical students (n = 110) of the 2023-2024 batch at All India Institute of Medical Sciences, Bhubaneswar, India. A briefing on the basic theories of concept mapping was carried out. Students participated in a structured concept mapping session focusing on the mechanism of muscle contraction. Students (two students in a group) created concept maps illustrating relationships between neuronal architecture, action potentials, neuromuscular transmission, sarcotubular system, excitation-contraction coupling, and muscle contraction processes. Data collection included digital submissions of concept maps and structured feedback questionnaires. Two faculty members evaluated the concept maps, and student feedback was analyzed using quantitative and qualitative approaches. Results A total of 110 first-year undergraduate medical students participated in the study and created 55 concept maps. The students scored 17.32 ± 1.7 out of 20 maximum achievable scores, which corresponds to an average of 86.59%. Seventy-five (68.18%) students rated the technique as excellent, 32 (29.09%) found it good, and only three (2.73%) rated it as average. Ninety-eight (88.8%) students strongly agreed or agreed that it provided a practical learning experience and found it refreshing compared to traditional lectures. Additionally, 102 (92.7%) students acknowledged its ability to stimulate creative thinking, and 102 (92.7%) also reported effective collaboration with peers. According to 82 (74.5%)students, concept mapping also facilitated in-depth knowledge acquisition, demonstrating its effectiveness in promoting active, engaging, and collaborative learning. Qualitative analysis revealed that concept mapping helped the students organize complex information, encourage critical thinking, improve retention through visual learning, promote collaborative knowledge-building, and facilitate self-assessment of their understanding. Conclusion Concept mapping can be used as apedagogical tool for teaching complex neuromuscular physiology concepts to first-year medical students. The technique can bridge the gap between fragmented knowledge and integrated understanding while promoting active learning and critical thinking. The majority of the students rated it as excellent or good. It significantly enhances engagement, creative thinking, and deeper subject understanding compared to traditional lectures. Qualitative feedback underscores its role in improving comprehension of complex concepts and critical thinking skills.

Assessment of ventricular mechanics by means of myocardial work in patients undergoing physiologic versus right ventricular apical stimulation:a prospective study

Abstract Introduction Left bundle branch area (LBBA) pacing has recently gained widespread acceptance in patients that need pacemaker (PM) stimulation. This type of physiologic pacing may improve ventricular mechanical contraction and reduce the risk of PM-induced left ventricular (LV) dysfunction. Myocardial work (MW), a new tool, based on the analysis of pressure-strain loops, combines the information from speckle tracking strain imaging and non-invasive estimate of LV pressure. It provides a better characterization of LV performance and detection of LV myocardial dysfunction. The aim of this study was to compare MW after PM implantation in LBBA pacing patients (group 1) vs right ventricular apical (RVA, group 2) pacing patients. A control group (group 3) was also enrolled for establishing MW normal values. Methods We studied 30 patients (56% females, age 80 y, IQR 75-84) who underwent PM implantation and had a high probability of a high % of stimulation and 21 controls (57% females, age 79 y, IQR 73-85, p=0.46 and p=0.73 respectively) Patients were prospectively included at two large university hospitals from March 23 until February 24. The mode of stimulation was chosen at their physician’s discretion. Exclusion criteria included LVEF <50% or significant structural or valvular disease. We compared MW parameters between group 1 and group 2 before PM implantation, and 24 hours and 3 months post PM implantation by Kruskal Wallis test. Additionally, we compared MW parameters between group 3 and PM patients after 24 hours using U Mann-Whitney test. Results The % of stimulation was 93%±12. There were no differences in baseline characteristics between group 1 (n=21, 71%) and group 2. At baseline, global work index (GWI), global constructive work (GCW), global wasted work (GWW) or global work efficiency (GWE) did not differ significantly between Group 1 and 2. At 24 hours, all the components of MW significantly improved in Group 1 and 2 and were maintained at 3 months, but the improvement was notably greater in Group 1 (see Table 1). Group 3 and PM patients were similar at baseline (HTN, 86% vs 80%, p=0.7197; DM, 29% vs 30%, p=0.91; coronary artery disease, 14% vs 10%, p=0.68; sinus rhythm, 76% vs 83%, p=0.68). Comparing group 3 and PM at 24 h, GWI, GCW, GWE was significantly higher in group 3 (p=0.0023, p=0.009, p=<0.0001) and GWW was significantly lower (p=<0.0001). Regarding the type of stimulation, MW parameters values in Group 1 were still lower than in Group 3 but clearly better than Group 2 values (see Figure 1). Discussion Our results showed that MW parameters improved to a greater extent in LBBA compared to RVA pacing immediately and after 3 months of PM implantation. Compared to our control group, LBBA pacing group achieved better performance than RVA pacing patients, yielding a more coordinated LV contraction. Nevertheless, more studies are needed to clarify if LBBA pacing causes less PM induced LV dysfunction compared to RVA pacing.

Genomic insights into Aspergillus tamarii TPD11: enhancing polyphyllin production and uncovering potential therapeutic applications.

The excavation and utilization of endophytic fungi from medicinal plants is highly important for the development of new drugs. The endophytic fungus Aspergillus tamarii TPD11, which was isolated and obtained by the authors in the previous stage, can produce a variety of polyphyllins with important potential applications in hemostasis, inflammation and antitumor activities; however, the genomic information of TPD11 is still unknown. In this study, we sequenced and assembled the whole genome of the endophytic fungus A. tamarii TPD11, resolved the genome evolutionary relationships of 24 Aspergillus strains, and phylogenetic analysis of the genomes of 16 strains revealed the evolutionary differences between Aspergillus and Penicillium and the mechanisms of genome expansion and contraction. CAZy annotation analysis revealed that TPD11 obtains nutrients mainly by ingesting starch from the host plant. TPD11 has a biosynthesis-related gene cluster for the synthesis of squalestatin S1, and the silencing of this biosynthesis-related gene cluster might increase the content of polyphyllin. Annotation of 11 UDP-glycosyltransferase genes helps to further reveal the biosynthetic pathway of polyphyllin. In addition, secondary metabolism gene cluster and CAZy analyses confirmed the potential probiotic, insecticidal and antimicrobial activities of TPD11 on host plants. This study reveals the intrinsic mechanism by which endophytic fungi increase the content of polyphyllin, which provides a basis for the synthetic synthesis of the natural product polyphyllin.

Investment Climate in the MENA Region: Are the Stabilisation Clauses in Foreign Direct Investment Agreements an Obstacle to Progress?

Abstract Foreign direct investment agreements between Western corporations and the countries of the Middle East and North Africa region (MENA) deserve attention. On one side, the MENA region is a developing region rich in natural resources and therefore a destination interesting to Western investors. On the other, the MENA region is perceived as a volatile and unstable investment environment. Therefore, international investment agreements in the said region often include stabilisation clauses that serve as contractual mechanisms protecting investors from any changes in the laws and acts of nationalisation and expropriation by the host states. As stabilisation clauses have serious adverse effects on the sovereignty of the host states and the development of human rights, this article will take a deeper view of the stabilisation practice and aim to reveal how this contractual mechanism might represent a Catch 22 with respect to the further development of the MENA region countries, especially with respect to human rights and internal governance.

Performance evaluation of the static thrust and efficiency of an actuated biomimetic jellyfish model

Abstract This study offers an in-depth analysis of a jellyfish-inspired robotic model designed to simulate the expansion and contraction mechanisms found in natural jellyfish. By employing variable Duty Cycles to replicate the cyclic motions of the jellyfish, the thrust generation of the model is measured through static thrust assessments using a load cell, while its efficiency is evaluated using the Thrust-to-Power Ratio, TPR. The findings indicate that increasing the amplitude of the motion leads to more complex wave patterns, which in turn reduce the peak-to-peak force generated. Specifically, a 25% increase in the A/D ratio results in a significant 30% decrease in static thrust. It was observed that higher actuation frequencies do not notably influence thrust generation when the A/D ratio remains constant. Importantly, the TPR was found to be highest at an A/D ratio of 0.10, with an increase of up to 6% observed at higher Duty Cycles, suggesting that smaller actuation amplitudes are more efficient in enhancing thrust performance. These results highlight the critical role of amplitude in the efficiency of static thrust generation within a given Duty Cycle, emphasizing that lower amplitudes generally lead to higher efficiency. The study underscores the importance of optimizing both geometric and kinematic parameters for improved propulsion performance. It suggests that future research should focus on fine-tuning A/D ratios and Duty Cycles, as well as exploring alternative geometric configurations and materials to further enhance the hydrodynamic performance of jellyfish-inspired robots. The insights gained from this study provide a valuable foundation for developing more efficient and effective bio-inspired underwater vehicles.

Technology and new work

The article aims to evaluate, through an evolutionary overview of social achievements related to labor law, the impact of reducing working hours on the productivity and health of Brazilian workers. Specifically, it seeks to answer the following questions: Will worker productivity increase with a temporary reduction in working hours? Are there positive health impacts for workers who experience a reduction in working hours? To address these questions, the hypothetical-deductive method was used. The methodological approach was based on historical-typological analysis, with the interpretation framed through a sociological lens. The research, qualitative in nature, employs bibliographic and documentary sources on the topic, with a primarily explanatory objective. The study highlights the need for closer monitoring of labor relations, focusing on the enhancement of human work, particularly in the context of current technological advancements. It argues that personal development can lead to increased productive capacity and a greater balance in the work-life relationship, thereby fostering continuous evolution. To achieve this, public policies aimed at enhancing the employability of Brazilian workers will be necessary, alongside the involvement of representative groups through collective labor law. These efforts should focus on developing effective contractual mechanisms to improve labor quality and drive economic development.

Is It Possible to Regenerate the Underactive Detrusor? Part 1 Molecular and Stem Cell Therapies Targeting the Urinary Bladder and Neural Axis ICI-RS 2024.

Detrusor muscle weakness is commonly noted on urodynamics in patients with refractory voiding difficulty. No approved therapies have been proven to augment the strength of a detrusor voiding contraction. This subject was discussed by a think-tank at the International Consultation on Incontinence- Research Society (ICI-RS) meeting held in Bristol, June 2024. The discussions of the think-tank are being published in two parts. This first part discusses molecular and stem cell therapies targeting the urinary bladder and the neural axis. Senescence of the urothelium and extracellular ATP acting through P2X3 receptors might be important in detrusor underactivity. Several molecules such as parasympathomimetics, acotiamide, ASP8302, neurokinin-2 agonists have been explored but none has shown unequivocal clinical benefit. Different stem cell therapy approaches have been used, chiefly in neurogenic dysfunction, with some studies showing benefit. Molecular targets for the neural axis have included TRPV-4, Bombesin, and serotoninergic receptors and TAC-302 which induces neurite growth. Several options are currently being pursued in the search for an elusive molecular or stem cell option for enhancing the power of the detrusor muscle. These encompass a wide range of approaches that target each aspect of the contraction mechanism including the urothelium of bladder and urethra, myocyte, and neural pathways. While none of these have shown unequivocal clinical utility, some appear promising. Lessons from other fields of medicine might prove instructive. Not necessary. Not a clinical trial.

Актуальные вопросы правового регулирования трудовой функции

The article considers the importance of legal regulation of labor function for the science of labor law and law enforcement today. The analysis of legislation, positions of state authorities, doctrinal provisions and current court practice proves that at the moment at different stages of labor relations in the legal regulation of labor function there are still significant gaps. The conclusion is formulated that it is necessary to specify a number of provisions of labor legislation concerning the documents fixing and changing the labor function of a particular employee, the use of contractual mechanism of changing the labor function.

Mechanisms for Ensuring Professional Responsibility of Medical Workers through Contractual Means

This article examines the use of contractual mechanisms to promote and enforce professional responsibility among medical workers. As healthcare systems face increasing pressures and scrutiny, there is a growing need for effective ways to ensure high standards of care and ethical conduct. The study analyzes how employment contracts, codes of conduct, and other formal agreements can be structured to clearly delineate professional expectations, incentivize best practices, and provide accountability. Key contractual elements explored include specific performance metrics, ongoing training requirements, incident reporting protocols, and disciplinary procedures. The potential benefits and limitations of relying on contractual means to shape professional behavior are critically assessed. While contracts alone cannot guarantee responsible conduct, when thoughtfully designed and consistently enforced, they can serve as powerful tools for health organizations to uphold professional standards and protect patient welfare.