Prime Mechanism Research Articles

The Open Society and Its Complexities

<i>The Open Society and Its Complexities</i>

Insights into Long-Term Acclimation Strategies of Grapevines (Vitis vinifera L.) in Response to Multi-Decadal Cyclical Drought

Changing climatic conditions across Australia’s viticulture regions is placing increasing pressure on resources such as water and energy for irrigation. Therefore, there is a pressing need to identify superior drought tolerant grapevine clones by exploring the extensive genetic diversity of early European clones in old vineyards. Previously, in a field trial, we identified drought-tolerant (DT) dry-farmed Cabernet Sauvignon clones that had higher intrinsic water use efficiency (WUEi) under prolonged soil moisture deficiency compared to drought-sensitive (DS) clones. To investigate whether the field-grown clones have been primed and confer the drought-tolerant phenotypes to their subsequent vegetative progenies, we evaluated the drought responses of DT and DS progenies under two sequential drought events in a glasshouse alongside progenies of commercial clones. The DT clonal progenies exhibited improved gas exchange, photosynthetic performance and WUEi under recurrent drought events relative to DS clonal progenies. Concentration of a natural priming agent, γ-amino butyric acid (GABA), was significantly higher in DT progenies relative to other progenies under drought. Although DT and commercial clones displayed similar drought acclimation responses, their underlying hydraulic, stomatal and photosynthetic regulatory mechanisms were quite distinct. Our study provides fundamental insights into potential intergenerational priming mechanisms in grapevine.

Priming Sentence Production in Older Adults: Evidence for Preserved Implicit Learning

ABSTRACT Purpose Structural priming- speakers’ unconscious tendency to echo previously encountered message-structure mappings - is thought to reflect the processes of implicit language learning that occur throughout the lifespan. Recently, structural priming has also been used as a means to facilitate language re-learning in age-related language disorders such as aphasia. However, little evidence is available on whether structural priming remains effective in healthy aging, limiting clinical translation of the structural priming paradigm. This study examined the impact of aging on the strength and longevity of abstract structural priming and lexical boost effects. Method Twenty-four young and 24 older adults participated in a collaborative picture-matching task where the participant and experimenter took turns describing picture cards using transitive and dative sentences. In Experiment 1, a target was elicited immediately following a prime (0-lag), whereas two filler items were embedded between a prime and a target sentence in Experiment 2 (2-lag) to examine longer-term priming effects. In both experiments, the verb was repeated for half of the prime-target pairs to examine lexical boost on structural priming. Results At immediate priming, older adults showed both abstract structural priming and lexical boost in transitives and datives, similar to young adults. At longer-term priming, only abstract priming effects were significant in both groups of participants, with no evidence of age-related reduction in priming. Conclusions Both lexically-specific and independent mechanisms of structural priming remain resilient in older adults, supporting the view that structural priming reflects life-long language learning. Further, the findings provide empirical basis for applying structural priming to elderly clinical populations.

Disclosing the molecular basis of salinity priming in olive trees using proteogenomic model discovery.

Plant responses to salinity are becoming increasingly understood, however, salt priming mechanisms remain unclear, especially in perennial fruit trees. Herein, we showed that low-salt pre-exposure primes olive (Olea europaea) plants against high salinity stress. We then performed a proteogenomic study to characterize priming responses in olive roots and leaves. Integration of transcriptomic and proteomic data along with metabolic data revealed robust salinity changes that exhibit distinct or overlapping patterns in olive tissues, among which we focused on sugar regulation. Using the multi-crossed -omics data set, we showed that major differences between primed and nonprimed tissues are mainly associated with hormone signaling and defense-related interactions. We identified multiple genes and proteins, including known and putative regulators, that reported significant proteomic and transcriptomic changes between primed and nonprimed plants. Evidence also supported the notion that protein post-translational modifications, notably phosphorylations, carbonylations and S-nitrosylations, promote salt priming. The proteome and transcriptome abundance atlas uncovered alterations between mRNA and protein quantities within tissues and salinity conditions. Proteogenomic-driven causal model discovery also unveiled key interaction networks involved in salt priming. Data generated in this study are important resources for understanding salt priming in olive tree and facilitating proteogenomic research in plant physiology.

Social Networks as a Perpetual Vehicle for Migration

Migration processes have accelerated in the past few decades all over the world, and pose major social and political challenges. The paper aims to discuss the constant importance of personal networks in these movements. It addresses the phenomenon of networks viewed as a prime social mechanism which amplifies and directs migration flows. Close attention has been paid to the variety of networks in which migrants operate in the host country, and the specific nature of the illegal border-crossing networks. The paper is based on relevant monographs and research data.

Induction and potential molecular mechanism of the enhanced immune response in Procambarus clarkii after secondary encountered with Aeromonas veronii

For a long time, it was believed that invertebrates do not possess acquired immunity and mainly rely on innate immunity for protection against pathogens infection. However, an increasing number of studies have suggested that some form of “immune memory” can be initiated in invertebrates after primary exposure to the pathogen, which was defined as “specific immune priming”. In the present study, two experiments were carried out to determine whether specific immune priming can be induced in crayfish (Procambarus clarkii) by Aeromonas veronii, if so, to identify the underlying mechanism. Once being “preimmunization” by formalin-killed A. veronii, the survival rate, in vitro antibacterial activity and haemocyte phagocytosis rate of crayfish were enhanced, which indicated that better immune protection was obtained. Furthermore, at some time points, the expression of antimicrobial peptide (AMP) and Down syndrome cell adhesion molecule (Dscam) genes was significantly higher in P. clarkii individuals that underwent stimulation twice than in those that were only stimulated once. Taken together, the results suggest that enhanced specific immune protection can be obtained in primed crayfish and that the Dscam molecule, haemocyte phagocytosis function, and AMPs may be involved in this immune priming. The present study provides a better understanding of the molecular mechanism of immune priming in invertebrates.

Improving dry machining performance of surface modified cutting tools through combined effect of texture and TiN-WS2 coating

In this research work, investigation on different surface modification techniques on cutting tools such as TiN-WS2 coating (T2), textured cutting tool (T3) and TiN-WS2 coated textured tool (T4) in dry machining of Ti-6Al-4V has been carried out and compared with commercial tool (T1). The presence of (002) orientation corresponding WS2 was observed in the diffraction pattern and this in turn was said to provide lubricity properties to the coatings. The cutting force generated during machining was reduced by 14 % for T4 tool when compared to T1. The T4 tool showed an increase in the shear angle by 7.5 % and decrease in coefficient of friction by 47 %. The prime mechanism attributed to the better performance of cutting tool was reduced tool-chip contact length. The T4 tools showed a 13 % reduction in tool-chip contact length when compared to commercial tools. The tool life of the T2, T3 and T4 tools was improved by 35 %, 29 % and 43 % respectively in comparison to commercial tools. The TiN-WS2 coatings, through its effective lubricity properties have said to nullify the effect of coating affinity between the tool and workpiece as evidenced by the absence of built-up edge on the tool rake face while machining using TiN-WS2 coated tool. The combined effect of TiN-WS2 coatings and surface textures have resulted in better machining characteristics, suggesting it as a suitable surface modification for dry machining of Ti-6Al-4V.

Inflammatory Cytokines That Enhance Antigen Responsiveness of Naïve CD8+ T Lymphocytes Modulate Chromatin Accessibility of Genes Impacted by Antigen Stimulation.

Naïve CD8+ T lymphocytes exposed to certain inflammatory cytokines undergo proliferation and display increased sensitivity to antigens. Such 'cytokine priming' can promote the activation of potentially autoreactive and antitumor CD8+ T cells by weak tissue antigens and tumor antigens. To elucidate the molecular mechanisms of cytokine priming, naïve PMEL-1 TCR transgenic CD8+ T lymphocytes were stimulated with IL-15 and IL-21, and chromatin accessibility was assessed using the assay for transposase-accessible chromatin (ATAC) sequencing. PMEL-1 cells stimulated by the cognate antigenic peptide mgp10025-33 served as controls. Cytokine-primed cells showed a limited number of opening and closing chromatin accessibility peaks compared to antigen-stimulated cells. However, the ATACseq peaks in cytokine-primed cells substantially overlapped with those of antigen-stimulated cells and mapped to several genes implicated in T cell signaling, activation, effector differentiation, negative regulation and exhaustion. Nonetheless, the expression of most of these genes was remarkably different between cytokine-primed and antigen-stimulated cells. In addition, cytokine priming impacted the expression of several genes following antigen stimulation in a synergistic or antagonistic manner. Our findings indicate that chromatin accessibility changes in cytokine-primed naïve CD8+ T cells not only underlie their increased antigen responsiveness but may also enhance their functional fitness by reducing exhaustion without compromising regulatory controls.

Complementary roles for auxin and auxin signalling revealed by reverse engineering lateral root stable prebranch site formation.

Priming is the process through which periodic elevations in auxin signalling prepattern future sites for lateral root formation, called prebranch sites. Thus far, the extent to which elevations in auxin concentration and/or auxin signalling are required for priming and prebranch site formation has remained a matter of debate. Recently, we discovered a reflux-and-growth mechanism for priming generating periodic elevations in auxin concentration that subsequently dissipate. Here, we reverse engineer a mechanism for prebranch site formation that translates these transient elevations into a persistent increase in auxin signalling, resolving the prior debate into a two-step process of auxin concentration-mediated initial signal and auxin signalling capacity-mediated memorization. A crucial aspect of the prebranch site formation mechanism is its activation in response to time-integrated rather than instantaneous auxin signalling. The proposed mechanism is demonstrated to be consistent with prebranch site auxin signalling dynamics, lateral inhibition, and symmetry-breaking mechanisms and perturbations in auxin homeostasis.

IKKβ primes inflammasome formation by recruiting NLRP3 to the trans-Golgi network

The NLRP3 inflammasome plays a central role in antimicrobial defense as well as in the context of sterile inflammatory conditions. NLRP3 activity is governed by two independent signals: the first signal primes NLRP3, rendering it responsive to the second signal, which then triggers inflammasome formation. Our understanding of how NLRP3 priming contributes to inflammasome activation remains limited. Here, we show that IKKβ, a kinase activated during priming, induces recruitment of NLRP3 to phosphatidylinositol-4-phosphate (PI4P), a phospholipid enriched on the trans-Golgi network. NEK7, a mitotic spindle kinase that had previously been thought to be indispensable for NLRP3 activation, was redundant for inflammasome formation when IKKβ recruited NLRP3 to PI4P. Studying iPSC-derived human macrophages revealed that the IKKβ-mediated NEK7-independent pathway constitutes the predominant NLRP3 priming mechanism in human myeloid cells. Our results suggest that PI4P binding represents a primed state into which NLRP3 is brought by IKKβ activity.

Properties of compression molded ultra-high molecular weight polyethylene: effects of varying process conditions

Abstract Ultrahigh molecular weight polyethylene (UHMWPE) has been extensively used in various tribological systems because of its outstanding tribological properties and excellent overall performance. Compression molding is the main molding method for UHMWPE, and the process parameters of molding have a profound effect on its material properties. In this study, three groups of UHMWPE samples were prepared, and their physical, mechanical, and tribological properties under different molding process parameters were examined—with a particular focus on the frictional and wear behavior of the material under various heating-temperatures, pressing-temperatures and pressures—and the friction and wear mechanisms of UHMWPE were analysed. Studies have shown that the rise in heating-temperature promotes the diffusion of polymer chains, resulting in an increased friction coefficient and wear loss of UHMWPE. The main wear mechanism switches from plastic deformation to fatigue wear. With an increase in the pressuring-temperature, the friction coefficient first increases and then decreases, while the wear loss increases, and the dominant wear mechanism switches from fatigue wear and plastic flow to plastic flow. With an increase in pressure, the friction coefficient and wear loss first decrease and then increase, and the prime wear mechanism changes from plastic deformation and fatigue wear to fatigue wear.

Special issue: Vocal accommodation in speech communication

This introductory article for the Special Issue on Vocal Accommodation in Speech Communication provides an overview of prevailing theories of vocal accommodation and summarizes the ten papers in the collection. Communication Accommodation Theory focusses on social factors evoking accent convergence or divergence, while the Interactive Alignment Model proposes cognitive integration of perception and production as an automatic priming mechanism driving convergence language production. Recent research including most of the papers in this Special Issue indicates that a hybrid or interactive synergy model provides a more comprehensive account of observed patterns of phonetic convergence than purely automatic mechanisms. Some of the fundamental questions that this special collection aimed to cover concerned (1) the nature of vocal accommodation in terms of underlying mechanisms and social functions in human–human and human–computer interaction; (2) the effect of task-specific and talker-specific characteristics (gender, age, personality, linguistic and cultural background, role in interaction) on degree and direction of convergence towards human and computer interlocutors; (3) integration of articulatory, perceptual, neurocognitive, and/or multimodal data to the analysis of acoustic accommodation in interactive and non-interactive speech tasks; and (4) the contribution of short/long-term accommodation in human–human and human–computer interactions to the diffusion of linguistic innovation and ultimately language variation and change.

Coastal barrier morphogenic categories in Mississippi Delta plain development

Coastal barriers are defined as shore-parallel, aggraded-prograded supratidal beach ridge sets and individual single ridge-landforms, composed of sand and/or gravel. Island, spit, and mainland barriers represent the three major topographic subdivisions. In terms of bathymetric and hydrographic characteristics, bottom sediment types and associated habitats, barriers separate the marine offshore from inshore facies. This definition is not uniformly accepted and applied in the literature. Barriers impose reduced salinities inshore, diminish storm impact and moderate inland inundation, protect critical habitats and may mark ancient longshore transport directions, shore and sea-level positions. A wide range of dimensions, topography, and other factors characterize mainland strandplains. Shoal aggradation appears to be the prime island-forming mechanism, followed by accretion around semi-consolidated, elevated cores. Smaller islands may also form by barrier spit fragmentation. Detachment of dune ridges from the mainland shore is an often cited but at best unlikely mode of island genesis. Review of the literature and remote-sensed images created new insights, utilized barrier definitions and genetic theories, as applied in the Mississippi Delta Complex. A three-stage concept, involving dune ridge or barrier spit-detachment by marine encroachment and landward shift of fragmented transgressive islands forms the basis of a recent invited Marine Geology research article. That model assumes spit-growth from delta headlands, followed by splintering into landward-migrating islands. Submerged, they become permanent shoals. For several reasons, this “island-cycle” hypothesis is questionable and a reassessment of the geomorphic and stratigraphic context of this delta coast is required.Contradicting the geomorphic setting of assumed Stage 1, key component of the cycle-model, evidence is lacking for subrecent delta lobes and erosional headland in spawning barrier islands. In addition, the delta headland-flanking islands reveal only progradational (regressive) development and in situ disintegration,. Transgressive landward migrations is known only from the Chandeleur chain's prehistory. Sand from decaying Terrebonne-Lafourche 6 Subdelta headland was the dominant source for the Caminada Strandplain and for the shoal /or island predecessor of Grand Isle. Wave refraction around an inferred Lafourche delta lobe (dated provisionally between ∼2900 - ∼ 2300 yrs. BP) and its decayed erosional headland remnant resulted in diametric reversal of littoral sand transport. An earlier proferred ∼750–300 yrs. BP Caminada Strandplain age range is now replaced by a loosely defined ∼2200- to- ∼800 yrs. BP value, based on ages of Grand Isle and reworked delta hinterland sediments incorporated into the Strandplain.

Endophytic Paenibacillus polymyxa LMG27872 inhibits Meloidogyne incognita parasitism, promoting tomato growth through a dose-dependent effect.

The root-knot nematode, Meloidogyne incognita, is a major pest in tomato production. Paenibacillus polymyxa, which is primarily found in soil and colonizing roots, is considered a successful biocontrol organism against many pathogens. To evaluate the biocontrol capacity of P. polymyxa LMG27872 against M. incognita in tomato, experiments were conducted both in vitro and in vivo. A dose-response effect [30, 50, and 100% (108 CFU/mL)] of bacterial suspensions (BSs) on growth and tomato susceptibility to M. incognita with soil drenching as a mode of application was first evaluated. The results show that the biological efficacy of P. polymyxa LMG27872 against M. incognita parasitism in tomato was dose-dependent. A significantly reduced number of galls, egg-laying females (ELF), and second-stage juveniles (J2) were observed in BS-treated plants, in a dose-dependent manner. The effect of P. polymyxa on tomato growth was also dose-dependent. A high dose of BSs had a negative effect on growth; however, this negative effect was not observed when the BS-treated plants were challenged with M. incognita, indicating tolerance or a defense priming mechanism. In subsequent in vivo experiments, the direct effect of BSs was evaluated on J2 mortality and egg hatching of M. incognita. The effect of BS on J2 mortality was observed from 12 to 24 h, whereby M. incognita J2 was significantly inhibited by the BS treatment. The effect of P. polymyxa on M. incognita egg hatching was also dependent on the BS dose. The results show a potential of P. polymyxa LMG27872 to protect plants from nematode parasitism and its implementation in integrated nematode management suitable for organic productions.

Abstract A39: Targeting tumor-microenvironmental dependencies as a therapeutic approach in Chronic Lymphocytic Leukemia (CLL)

Abstract Interaction between CLL and its microenvironment is a major factor in drug resistance. Bone marrow derived stroma cells provide a protective and supportive niche to allow CLL cells to survive drug-induced apoptosis. Therefore, a current challenge is to develop new strategies to target the microenvironment. To understand the role of tumor microenvironment in CLL survival, we studied conditioned media (CM) derived from NKTert cells. Human NKTert stroma cells provide a survival advantage to primary CLL cells by protecting them from spontaneous apoptosis in ex-vivo culture. We identified three cytokines (IL6, IL8 and CCL2) from CM as candidates for mediating survival. We exposed CLL cells to neutralizing antibodies against each cytokine and found significant decrease in CLL viability in presence of anti-CCL2 suggesting its role in CLL survival. BH3 profiling revealed an increase in apoptotic priming with anti-CCL2 treatment as well. Using dynamic BH3 profiling (DBP), we found that anti-CCL2 increased dependence on BCL-2 as well as BCL-xL in primary CLL cells suggesting a potential role of CCL2 on CLL survival. To our surprise, we also identified a CLL group (CM independent, CMI) that were resilient to cell death in the absence of stroma support in ex-vivo culture. In addition to this, anti-CCL2 antibody showed no effect on their survival. Next, we exposed CMI CLL cells to venetoclax and found that these CLL cells were less sensitive to apoptosis compared to the CLL samples that are stroma dependent (CM dependent, CMD). BH3 profiling showed that CMI CLL cells are less primed for apoptosis. Consistent with our findings, DBP with anti-CCL2, venetoclax or the combination didn’t increase any drug induced apoptotic priming in CMI CLL samples. In our study, 80% of CMI harbor unmutated IgHV. To provide additional insight to the molecular mechanism, we performed western blot analyses for BCL-2 family proteins and found decreased BAX ad BCL-2 expression in CMI samples. This result suggests altered BCL-2 family proteins expression might be a mechanism for differential priming. Bulk RNA seq analysis revealed upregulation of focal adhesion genes and Ras signaling pathway in CMI samples. In summary, our study suggest that in the CMD subset of CLL, anti-CCL2 therapy enhances apoptotic priming and venetoclax efficacy. Furthermore, we have also functionally defined two biologically distinct CLL subgroups based on stroma dependence. Citation Format: Salma Parvin, Aditi Aryal, Shanye Yin, Matthew S. Davids, Geoffrey G. Fell, Catherine J. Wu, Anthony Letai. Targeting tumor-microenvironmental dependencies as a therapeutic approach in Chronic Lymphocytic Leukemia (CLL) [abstract]. In: Proceedings of the Third AACR International Meeting: Advances in Malignant Lymphoma: Maximizing the Basic-Translational Interface for Clinical Application; 2022 Jun 23-26; Boston, MA. Philadelphia (PA): AACR; Blood Cancer Discov 2022;3(5_Suppl):Abstract nr A39.

Negative priming effect from tree leaf and root residues with contrasting chemical composition

Changes in soil organic carbon (SOC) decomposition in response to fresh plant residue inputs (i.e., the priming effect) represent an important biogeochemical process controlling soil C dynamics. However, the variations in priming induced by the addition of leaf and root residues with different chemical compositions among tree species and their potential mechanisms are not yet well known. We conducted a 90-day incubation experiment and explored the responses of priming effect to the 13C-labelled leaf and root residues of Cyclobalanopsis glauca, Cunninghamia lanceolata, Acacia confusa and Manglietia fordiana and further examined the relationships between the priming and the chemical compositions of these tree residues. Our results show that tree residue inputs decreased native SOC mineralization by approximately 20–59%, and root residues induced more pronounced or comparable negative priming effects than leaf residues. Moreover, the intensities of the negative priming were greater in soils supplemented with C. glauca and C. lanceolata residues than in soils supplemented with A. confusa and M. fordiana residues. The magnitude of the negative priming effect was inversely correlated with K content in plant residues but positively correlated with lignin content, lignin/N ratio and C/N ratio. The structural equation model indicates that residue K content, lignin/N ratio, as well as plant-derived microbial biomass carbon (MBC) and dissolved organic carbon (DOC) were the key derivers of the priming, with these drivers together explaining approximately 82% of the variations in the cumulative priming effects. Overall, our study suggests that tree root residues generally induce a more negative priming than tree leaf residues, but the extent of priming varied with tree species. Chemical properties of tree residues can help to improve our understanding of the priming mechanisms that could lead to a more precise estimation of SOC dynamics.

Impacts of combined microphysical and land-surface uncertainties on convective clouds and precipitation in different weather regimes

Abstract. To reduce the underdispersion of precipitation in convective-scale ensemble prediction systems, we investigate the relevance of microphysical and land-surface uncertainties for convective-scale predictability. We use three different initial soil moisture fields and study the response of convective precipitation to varying cloud condensation nuclei (CCN) concentrations and different shape parameters of the cloud droplet size distribution (CDSD) by applying a novel combined-perturbation strategy. Using the new ICOsahedral Non-hydrostatic (ICON) model, we construct a 60-member ensemble for cases with summertime convection under weak and strong synoptic-scale forcing over central Europe. We find a systematic positive soil moisture–precipitation feedback for all cases, regardless of the type of synoptic forcing, and a stronger response of precipitation to different CCN concentrations and shape parameters for weak forcing than for strong forcing. While the days with weak forcing show a systematic decrease in precipitation with increasing aerosol loading, days with strong forcing also show nonsystematic responses for some values of the shape parameters. The large magnitudes of precipitation deviations compared to a reference simulation ranging between −23 % and +18 % demonstrate that the uncertainties investigated here and, in particular, their collective effect are highly relevant for quantitative precipitation forecasting of summertime convection in central Europe. A rainwater budget analysis is used to identify the dominating source and sink terms and their response to the uncertainties applied in this study. Results also show a dominating cold-rain process for all cases and a strong but mostly nonsystematic impact on the release of latent heat, which is considered to be the prime mechanism for the upscale growth of small errors affecting the predictability of convective systems. The combined ensemble spread when accounting for all three uncertainties lies in the same range as the ones from an operational convective-scale ensemble prediction system with 20 members determined in previous studies. This indicates that the combination of different perturbations used in our study may be suitable for ensemble forecasting and that this method should be evaluated against other sources of uncertainty.

Parental salt priming improves the low temperature tolerance in wheat offspring via modulating the seed proteome

Low temperature is one of the main abiotic stresses that inhibit wheat growth and development. To understand the physiological mechanism of salt priming induced low temperature tolerance and its transgenerational effects, the chlorophyl b-deficient mutant (ANK) and its wild type (WT) wheat were subjected to low temperature stress after parental salt priming. Salt priming significantly decreased the levels of superoxide anions, hydrogen peroxide and malondialdehyde in both parental and offspring plants under low temperature. The catalase activity in parental wheat and activities of dehydroascorbate reductase and glutathione reductase in the offspring were significantly increased by salt priming under low temperature. Meanwhile, salt priming contributed to mantaining the integrity of chloroplast structure and relatively higher net photosynthetic rate (Pn) in both generations under low temperature. Salt priming also improved the carbohydrate metabolism enzyme activities of parental and offspring plants, such as phosphoglucomutase, fructokinase and sucrose synthase. In addition, ANK plants had significantly higher carbohydrate metabolism enzyme activities than WT plants. The differential expressed proteins (DEP) in seeds of two genotypes under salt priming were mainly related to homeostasis, electron transfer activity, photosynthesis and carbohydrate metabolism. Correlation network analysis showed that the expression of DEP under salt priming was significantly correlated to sucrose concentration and cytoplasmic peroxidase (POX) activity in WT, while that was correlated to various carbohydrate metabolism enzyme activities in ANK plants. These results indicated that the parental salt priming induced modulations of seed proteome regulated the ROS metabolism, photosynthetic carbon assimilation and carbohydrate metabolism, hence enhancing the low temperature tolerance in offspring wheat.

Chemical priming of natural killer cells with branched polyethylenimine for cancer immunotherapy

BackgroundDue to their powerful immune surveillance activity and ability to kill and clear cancer cells, natural killer (NK) cells are an emerging anticancer immunotherapeutic agent. Therefore, there is much interest...

Negative evidence on the transgenerational inheritance of defense priming in Arabidopsis thaliana

Defense priming allows plants to enhance their immune responses to subsequent pathogen challenges. Recent reports suggested that acquired resistances in parental generation can be inherited into descendants. Although epigenetic mechanisms are plausible tools enabling the transmission of information or phenotypic traits induced by environmental cues across generations, the mechanism for the transgenerational inheritance of defense priming in plants has yet to be elucidated. With the initial aim to elucidate an epigenetic mechanism for the defense priming in plants, we reassessed the transgenerational inheritance of plant defense, however, could not observe any evidence supporting it. By using the same dipping method with previous reports, Arabidopsis was exposed repeatedly to Pseudomonas syringae pv tomato DC3000 (Pst DC3000) during vegetative or reproductive stages. Irrespective of the developmental stages of parental plants that received pathogen infection, the descendants did not exhibit primed resistance phenotypes, defense marker gene (PR1) expression, or elevated histone acetylation within PR1 chromatin. In assays using the pressure-infiltration method for infection, we obtained the same results as above. Thus, our results suggest that the previous observations on the transgenerational inheritance of defense priming in plants should be more extensively and carefully reassessed.