Mechanisms Of Cd Detoxification Research Articles

Wound healing and Cadmium detoxification in the earthworm Lumbricus terrestris - a potential case for coelomocytes?

Earthworms are affected by physical stress, like injury, and by exposure to xenobiotics, such as the toxic metal cadmium (Cd), which enters the environment mainly through industry and agriculture. The stress response to the single and the combination of both stressors was examined in regenerative and unharmed tissue of Lumbricus terrestris to reveal if the stress response to a natural insult like injury (amputation) interferes with Cd detoxification mechanisms. We characterized the roles of metallothionein 1 (MT1) and MT2 isoforms, heat shock protein 70 as well as immune biomarkers such as the toll-like receptors (TLR) single cysteine cluster TLR and multiple cysteine cluster TLR. The role of the activated transcription factors (ATFs) ATF2, ATF7, and the cAMP responsive element binding protein as putative regulatory intersection as well as a stress-dependent change of the essential trace elements zinc and calcium was analyzed. Phosphorylated AMP activated protein kinase, the cellular energy sensor, was measured to explore the energy demand, while the energy status was determined by detecting carbohydrate and protein levels. Taken together, we were able to show that injury rather than Cd is the driving force that separates the four treatment groups - Control, Cd exposure, Injury, Cd exposure and injury. Interestingly, we found that gene expression differed regarding the tissue section that was analyzed and we hypothesize that this is due to the migration of coelomocytes, earthworm immune cells, that take over a key role in protecting the organism from a variety of environmental challenges. Surprisingly, we discovered a role for MT1 in the response to multiple stressors and an isoform-specific function for the two newly characterized TLRs. In conclusion, we gathered novel information on the relation of innate immunity, wound healing, and Cd detoxification mechanisms in earthworms.

Cadmium toxicity in medicinal plants: An overview of the tolerance strategies, biotechnological and omics approaches to alleviate metal stress.

Medicinal plants, an important source of herbal medicine, are gaining more demand with the growing human needs in recent times. However, these medicinal plants have been recognized as one of the possible sources of heavy metal toxicity in humans as these medicinal plants are exposed to cadmium-rich soil and water because of extensive industrial and agricultural operations. Cadmium (Cd) is an extremely hazardous metal that has a deleterious impact on plant development and productivity. These plants uptake Cd by symplastic, apoplastic, or via specialized transporters such as HMA, MTPs, NRAMP, ZIP, and ZRT-IRT-like proteins. Cd exerts its effect by producing reactive oxygen species (ROS) and interfere with a range of metabolic and physiological pathways. Studies have shown that it has detrimental effects on various plant growth stages like germination, vegetative and reproductive stages by analyzing the anatomical, morphological and biochemical changes (changes in photosynthetic machinery and membrane permeability). Also, plants respond to Cd toxicity by using various enzymatic and non-enzymatic antioxidant systems. Furthermore, the ROS generated due to the heavy metal stress alters the genes that are actively involved in signal transduction. Thus, the biosynthetic pathway of the important secondary metabolite is altered thereby affecting the synthesis of secondary metabolites either by enhancing or suppressing the metabolite production. The present review discusses the abundance of Cd and its incorporation, accumulation and translocation by plants, phytotoxic implications, and morphological, physiological, biochemical and molecular responses of medicinal plants to Cd toxicity. It explains the Cd detoxification mechanisms exhibited by the medicinal plants and further discusses the omics and biotechnological strategies such as genetic engineering and gene editing CRISPR- Cas 9 approach to ameliorate the Cd stress.

Alleviation of cadmium phytotoxicity through melatonin modulated physiological functions, antioxidants, and metabolites in tomato (Solanum lycopersicum L.).

The rising concentration of cadmium (Cd) builds a harmful effect on human and plant health associated with food chain contagion. Melatonin (MT) is an indole compound. Hence, the experiment was conducted to understand the physiological and biochemical mechanism of Cd detoxification by exogenous MT in tomato. Pots were filled with 30 ppm of Cd spiked soil and different concentration of exogenous MT was given to the plants through seed treatment (250 ppm), foliar spray viz., 25, 50, and 100 ppm, and both, whereas the foliar spray was given at 30 days after transplanting (DAT) and 46 DAT. When the plants are exposed to Cd stress, it reduces the gas exchange characters. The results revealed that foliar spray of 25 ppm of exogenous MT recorded the highest photosynthetic rate, stomatal conductance, and osmotic potential. MT had a direct interaction with reactive oxygen species scavenging by elevating endogenous antioxidant enzymes as well as the metabolites in plants. The contribution of MT foliar spray of 25 ppm at 30 and 46 DAT can mitigate Cd stress and it has potential implications for ensuring food safety and food security in marginal agriculture.

Uptake of atmospherically deposited cadmium by leaves of vegetables: Subcellular localization by NanoSIMS and potential risks

Atmospherically deposited cadmium (Cd) may accumulate in plants through foliar uptake; however, the foliar uptake, accumulation, and distribution processes of Cd are still under discussion. Atmospherically deposited Cd was simulated using cadmium sulfide (CdS) with various particle sizes and solubility. Water spinach (Ipomoea aquatica Forsk, WS) and pak choi (Brassica chinensis L., PC) leaves were treated with suspensions of CdS nanoparticles (CdSN), which entered the leaves via the stomata. Cd concentrations of WS and PC leaves treated with 125 mg L−1 CdSN reached up to 39.8 and 11.0 mg kg−1, respectively, which are higher than the critical leaf concentration for toxicity. Slight changes were observed in fresh biomass, photosynthetic parameters, lipid peroxidation, and mineral nutrient uptake. Exposure concentration, rather than particle size or solubility, regulated the foliar uptake and accumulation of Cd. Subcellular and the high-resolution secondary ion mass spectrometry (NanoSIMS) results revealed that Cd was majorly stored in the soluble fraction and cell walls, which is an important Cd detoxification mechanism in leaves. The potential health risks associated with consuming CdS-containing vegetables were highlighted. These findings facilitate a better understanding of the fate of atmospheric Cd in plants, which is critical in ensuring food security.

Comparative physiological and transcriptomic analyses reveal ascorbate and glutathione coregulation of cadmium toxicity resistance in wheat genotypes

BackgroundCadmium (Cd) is a heavy metal with high toxicity that severely inhibits wheat growth and development. Cd easily accumulates in wheat kernels and enters the human food chain. Genetic variation in the resistance to Cd toxicity found in wheat genotypes emphasizes the complex response architecture. Understanding the Cd resistance mechanisms is crucial for combating Cd phytotoxicity and meeting the increasing daily food demand.ResultsUsing two wheat genotypes (Cd resistant and sensitive genotypes T207 and S276, respectively) with differing root growth responses to Cd, we conducted comparative physiological and transcriptomic analyses and exogenous application tests to evaluate Cd detoxification mechanisms. S276 accumulated more H2O2, O2−, and MDA than T207 under Cd toxicity. Catalase activity and levels of ascorbic acid (AsA) and glutathione (GSH) were greater, whereas superoxide dismutase (SOD) and peroxidase (POD) activities were lower in T207 than in S276. Transcriptomic analysis showed that the expression of RBOHA, RBOHC, and RBOHE was significantly increased under Cd toxicity, and two-thirds (22 genes) of the differentially expressed RBOH genes had higher expression levels in S276 than inT207. Cd toxicity reshaped the transcriptional profiling of the genes involving the AsA-GSH cycle, and a larger proportion (74.25%) of the corresponding differentially expressed genes showed higher expression in T207 than S276. The combined exogenous application of AsA and GSH alleviated Cd toxicity by scavenging excess ROS and coordinately promoting root length and branching, especially in S276.ConclusionsThe results indicated that the ROS homeostasis plays a key role in differential Cd resistance in wheat genotypes, and the AsA-GSH cycle fundamentally and vigorously influences wheat defense against Cd toxicity, providing insight into the physiological and transcriptional mechanisms underlying Cd detoxification.

Physiological role of CYP17A1-like in cadmium detoxification and its transcriptional regulation in the Pacific oyster, Crassostrea gigas

Cadmium (Cd) is one of the most harmful heavy metals due to its persistence and bioaccumulation through the food chains, posing health risks to human. Oysters can bioaccumulate and tolerate high concentrations of Cd, providing a great model for studying molecular mechanism of Cd detoxification. In a previous study, we identified two CYP genes, CYP17A1-like and CYP2C50, that were potentially involved in Cd detoxification in the Pacific oyster, Crassostrea gigas. In this work, we performed further investigations on their physiological roles in Cd detoxification through RNA interference (RNAi). After injection of double-stranded RNA (dsRNA) into the adductor muscle of oysters followed by Cd exposure for 7 days, we observed that the expressions of CYP17A1-like and CYP2C50 in interference group were significantly suppressed on day 3 compared with control group injected with PBS. Moreover, the mortality rate and Cd content in the CYP17A1-like dsRNA interference group (dsCYP17A1-like) was significantly higher than those of the control on day 3. Furthermore, the activities of antioxidant enzymes, including SOD, CAT, GST, were significantly increased in dsCYP17A1-like group, while were not changed in dsCYP2C50 group. More significant tissue damage was observed in gill and digestive gland of oysters in RNAi group than control group, demonstrating the critical role of CYP17A1-like in Cd detoxification. Dual luciferase reporter assay revealed three core regulatory elements of MTF-1 within promoter region of CYP17A1-like, suggesting the potential transcriptional regulation of CYP17A1-like by MTF-1 in oysters. This work demonstrated a critical role of CYP17A1-like in Cd detoxification in C. gigas and provided a new perspective toward unravelling detoxification mechanisms of bivalves under heavy metal stress.

Transcriptome Analysis and Expression Profiling of Molecular Responses to Cd Toxicity in Morchella spongiola

Morchella is a genus of fungi with the ability to concentrate Cd both in the fruit-body and mycelium. However, the molecular mechanisms conferring resistance to Cd stress in Morchella are unknown. Here, RNA-based transcriptomic sequencing was used to identify the genes and pathways involved in Cd tolerance in Morchella spongiola. 7444 differentially expressed genes (DEGs) were identified by cultivating M. spongiola in media containing 0.15, 0.90, or 1.50 mg/L Cd2+. The DEGs were divided into six sub-clusters based on their global expression profiles. GO enrichment analysis indicated that numerous DEGs were associated with catalytic activity, cell cycle control, and the ribosome. KEGG enrichment analysis showed that the main pathways under Cd stress were MAPK signaling, oxidative phosphorylation, pyruvate metabolism, and propanoate metabolism. In addition, several DEGs encoding ion transporters, enzymatic/non-enzymatic antioxidants, and transcription factors were identified. Based on these results, a preliminary gene regulatory network was firstly proposed to illustrate the molecular mechanisms of Cd detoxification in M. spongiola. These results provide valuable insights into the Cd tolerance mechanism of M. spongiola and constitute a robust foundation for further studies on detoxification mechanisms in macrofungi that could potentially lead to the development of new and improved fungal bioremediation strategies.

Comparative transcriptome analysis of the hyperaccumulator plant Phytolacca americana in response to cadmium stress.

The online version contains supplementary material available at 10.1007/s13205-021-02865-x.

Physiological, anatomical, and transcriptional responses of mulberry (Morus alba L.) to Cd stress in contaminated soil

Mulberry has been widely studied for its capacity to tolerate heavy metals. However, the anatomical and molecular response mechanisms of Cd detoxification and transportation in mulberry have not been fully elucidated. In this study, the anatomical characteristics, Cd and mineral element uptake and transport, and transcriptome profiling of mulberry were studied under Cd stress. The results showed that mulberry possessed strong detoxification and self-protection abilities against Cd stress. The growth and photosynthetic pigment contents of mulberry were only slightly affected when the soil Cd content was less than 37.0 mg/kg, while the Ca and Mg contents in the mulberry roots were clearly (p < 0.05) increased by 37.85%–40.87% and 36.63%–53.06% in 37.0–55.4 mg/kg Cd-contaminated soil. Meanwhile, the relationships between antioxidant enzyme activities, such as peroxidase, catalase, and ascorbate peroxidase, and Cd content in plants were positive. Furthermore, the structures of leaf cells, root and stem tissues were largely intact; simultaneously, the increase in osmiophilic particles and the dissolution of starch granules in mulberry leaves significantly responded to Cd stress. Clusters of Orthologous Groups of proteins (COG) and Gene Ontology (GO) classification analysis indicated that mulberry can enhance the catalytic activity, regulate the transport and metabolism of inorganic ions, and strengthen its antioxidant enzyme activity and defense mechanism to decrease Cd intoxication. Large numbers of differentially expressed genes associated with cell wall biosynthesis, antioxidant enzyme activities, glutathione metabolism, chelation, plant hormone signal transduction, and the mitogen-activated protein kinase (MAPK) signaling pathway were upregulated under Cd stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that plant hormone signal transduction was significantly (p < 0.05) enriched in roots, stems, and leaves of mulberry, and abscisic acid and ethylene can mediate MAPK signaling pathways to increase plant tolerance to Cd stress. The results suggested that the physiological, cellular and tissue, and transcriptional regulation of mulberry can facilitate its stress adaptation in Cd-contaminated soil.

Overexpression of FaHSP17.8-CII improves cadmium accumulation and tolerance in tall fescue shoots by promoting chloroplast stability and photosynthetic electron transfer of PSII

Genetic improvement could play a significant role in enhancing the Cd accumulation, translocation and tolerance in plants. In this study, for the first time, we constructed transgenic tall fescue overexpressing a class II (CII) sHSP gene FaHSP17.8-CII, which enhanced Cd tolerance and the root-to-shoot Cd translocation. After exposed to 400 μM CdCl2, two FaHSP17.8-CII overexpressing lines (OE#3 and OE#7) exhibited 30% and 40% more shoot fresh weight, respectively, relative to the wild-type (WT). Both transgenic lines showed higher tolerance to Cd, as evidenced by lower levels of electrolyte leakage and malondialdehyde compared to the WT plants under Cd stress. FaHSP17.8-CII overexpression increased shoot Cd contents 49–59% over the WT plants. The Cd translocation factor of root-to-shoot in OE grasses was 69–85% greater than WT under Cd stress. Furthermore, overexpression of FaHSP17.8-CII reduced Cd-induced damages of chloroplast ultra-structure and chlorophyll synthesis, and then improved photosystem II (PSII) function under Cd stress, which resulted in less reactive oxygen species (ROS) accumulation in OE grasses than that in WT exposed to Cd stress. The study suggests a novel FaHSP17.8-CII–PSII-ROS module to understand the mechanisms of Cd detoxification and tolerance, which provides a new strategy to improve phytoremediation efficiency in Cd-stressed grasses.

Uptake, accumulation and elimination of cadmium in a soil - Faba bean (Vicia faba) - Aphid (Aphis fabae) - Ladybird (Coccinella transversalis) food chain

Cadmium is a highly mobile toxic heavy metal and a serious hazard to the biosphere. We studied uptake, accumulation and elimination of cadmium in a soil - faba bean - aphid - ladybird food chain. The soil in the study was amended with Cd at concentrations 0, 5, 10, 20 and, 30 mg kg−1 (w/w). We noted significant Cd transfer in a dose-dependent manner. Cadmium biomagnified in faba bean roots and aphids while biominimized in ladybirds as revealed by their respective transfer coefficients. The concentration-dependent removal of Cd from aphids through excretion via honeydew as well as through pupal exuviae of ladybirds during metamorphosis links to possible mechanisms of Cd detoxification at these trophic levels, which regulates the bioaccumulation of Cd along the food chain. These findings press for the advance studies to find and understand the physiological pathways and mechanisms leading to bio-minimization of Cd across the food chain.

Nitric oxide induced Cd tolerance and phytoremediation potential of B. juncea by the modulation of antioxidant defense system and ROS detoxification.

The present study designed to illustrate correlation between cadmium induced stress and plant growth, photosynthetic pigments, morphological and physiological attributes. To study these parameters 2 weeks old seedling of B. juncea were subjected to 50µM Cd, 100µM Cd and 100µM SNP separately and in combination with SNP. After 96h, the treated plant were harvested to analyze the cellular homeostasis and metal tolerance mechanism via examining growth, stress parameters, enzymatic and non enzymatic antioxidants and expression level of NR. Higher level of Cd (100µM) significantly increased accumulation of reactive oxygen species and malonaldehyde content in comparison to 50µM Cd. Exogenous supplementation of SNP (100µM) to 50µM Cd treated plant had an additive effect on plant growth by improving the level of proline, photosynthetic pigments and activities of enzymatic antioxidants which was confirmed by histochemical staining for NADPH-d and NO fluorescence from DAF-DA staining in roots of B. juncea. Applying SNP to 50µM Cd exposed B. juncea roots enhanced NR activity by 1.36 folds and increased NO production by 1.12 folds than individual Cd treated roots. In addition, semi quantitative RT-PCR study revealed the induction of BjNR was more pronounced in 50µM Cd treated roots in comparison to 100µM Cd treated roots. The present finding revealed NO confers increased B. juncea tolerance to Cd stress by stimulation of antioxidants and reestablishment of cellular redox status. Different biochemical analysis showed that plant growth, photosynthetic pigment and antioxidants were positively correlated with NO and it's negatively correlated with oxidative stress biomarkers. Therefore, NO is gaseous signalling molecule with potential role in Cd detoxification mechanism in B. juncea.

Cadmium tolerance and detoxification in Myriophyllum aquaticum: physiological responses, chemical forms, and subcellular distribution.

Submerged macrophytes have been found to be promising in removing cadmium (Cd) from aquatic ecosystems; however, the mechanism of Cd detoxification in these plants is still poorly understood. In the present study, Cd chemical forms and subcellular distributing behaviors in Myriophyllum aquaticum and the physiological mechanism underlying M. aquaticum in response to Cd stress were explored. During the study, M. aquaticum was grown in a hydroponic system and was treated under different concentrations of Cd (0, 0.01, 0.05, 0.25, and 1.25mg/L) for 14days. The differential centrifugation suggested that most Cd was split in the soluble fraction (57.40-66.25%) and bound to the cell wall (24.92-38.57%). Furthermore, Cd in M. aquaticum was primarily present in NaCl-extractable Cd (51.76-91.15% in leaves and 58.71-84.76% in stems), followed by acetic acid-extractable Cd (5.17-22.42% in leaves and 9.54-16.56% in stems) and HCl-extractable Cd (0.80-12.23% in leaves and 3.56-18.87% in stems). The malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentrations in M. aquaticum were noticeably increased under each Cd concentration. The activities of catalase (CAT), guaiacol peroxidase (POD), and superoxide dismutase (SOD) in leaves were initially increased under relatively low concentrations of Cd but were decreased further with the increasing concentrations of Cd. The ascorbate (AsA), glutathione (GSH), and nitric oxide (NO) concentrations in stems increased with increasing Cd concentrations. Taken together, our results indicate that M. aquaticum can be used successfully for phytoremediation of Cd-contaminated water, and the detoxification mechanisms in M. aquaticum include enzymatic and non-enzymatic antioxidants, subcellular partitioning, and the formation of different chemical forms of Cd.

Cadmium resistance mechanisms of a functional strain Enterobacter sp. DNB-S2, isolated from black soil in Northeast China

Enterobacter sp. DNB-S2 is an efficient DBP-degrading bacterial strain, which was proved to be highly resistant to cadmium (MIC was 12.5 mM) and the involved mechanisms were verified in this study. The results showed the maximu values of extracellular adsorbed and intracellular accumulated Cd2+ contents accounted for 24.67% and 4.78% of the initial Cd2+ content in the medium. The extreme low intracellular Cd accumulation reduced the risk of cadmium interfering with normal cell metabolism. TEM exhibited cell structure of DNB-S2 kept intact even at high Cd concentrations. Some precipitation particles were formed inside cells, which were further identified to be Cd–O–P, Cd (OH)2, CdCO3, and CdS by XPS. Furthermore, Cd resistant genes were predicted on DNB-S2 genome, and the presence of 11 alkaline phosphatase encoding genes provided genetic basis for the formation of cadmium phosphate precipitation, which illustrated intracellular precipitation is one of the important intracellular Cd detoxification mechanisms in strain DNB-S2. Noteworthily, 8 efflux proteins were annotated possibly responsible for cadmium efflux and they were all predicted located on cytoplasmic membrane. In a word, integrity of cell structure, the formation of cadmium precipitation and efficient exclusion primarily constitute Cd tolerance mechanisms of strain DNB-S2. This strain exhibits promising application to the bioremediation of cadmium-DBP contaminated bites.

Heat shock pretreatment induced cadmium resistance in the nematode Caenorhabditis elegans is depend on transcription factors DAF-16 and HSF-1

Cadmium (Cd) exposure poses a serious environmental problem due to the metal’s bioaccumulation and difficult to eliminate from body. Understanding the mechanisms of Cd detoxification and resistance can provide insights into methods to protect against the damaging effects of the heavy metal. In the present study, we found that heat shock (HS) pretreatment increased Cd resistance of the nematode Caenorhabditis elegans by reducing the bagging phenotype and protecting the integrity of the intestinal barrier. HS pretreatment increased the expression of heat shock protein-16.2 (HSP-16.2) prior to Cd exposure, and HS-induced Cd resistance was absent in worms with hsp-16.2 loss-of-function mutation. Worm strain with daf-2(e1370) mutation presented enhanced HS-induced Cd resistance, which was eliminated in worm strains of daf-16(mu86) and hsf-1(sy441). HS pretreatment increased DAF-16 nuclear localization and HSF-1 granule formation prior to Cd exposure. DAF-16 and HSF-1 was essential in reducing bagging formation and protecting the integrity of intestinal barrier after HS pretreatment. In conclusion, the present study demonstrated that HS-induced Cd resistance in C. elegans is regulated by the DAF-16/FOXO and HSF-1 pathways through regulation of HSP-16.2 expression.

Heterologous expression of Zn-binding peptide RaZBP1 from Russula bresadolae does not overcome Zn and Cd detoxification mechanisms in Hebeloma mesophaeum.

Homeostatic mechanisms preventing the toxicity of heavy metal ions in cells involve, among others, compartmentalization and binding with peptidaceous ligands, particularly the cysteinyl-rich metallothioneins (MTs). We have previously shown that in natural conditions Zn-overaccumulating ectomycorrhizal (EM) fungus Russula bresadolae stores nearly 40% of Zn bound with cysteinyl- and hystidyl-containing RaZBP peptides, which resemble MTs, while the detoxification of Zn and Cd in EM Hebeloma mesophaeum relies upon compartmentalization in small vesicles and vacuoles, respectively. Here, we examined the performance of RaZBP1 gene expressed in H. mesophaeum mycelium with respect to handling of Zn and Cd. Expression of RaZBP1 impaired growth of the mycelium on low-Zn medium by 60%, the growth was partly ameliorated upon the addition of Zn and remained considerable up to 2mmol/L Zn, while the growth of the wild-type and control mycelia transformed with empty T-DNA was severely reduced in the presence of 0.5mmol/L Zn; furthermore, RaZBP1 slightly added to Cd tolerance in the range of Cd concentrations of 0.625 to 8μmol/L. Staining of Zn- or Cd-exposed hyphal cells with Zn- or Cd-specific fluorescent tracers did not indicate that the expression of RaZBP1 would redirect the flow of the metals away from their innate sinks. Size exclusion chromatography of extracted metal species revealed that the complexes corresponding to Zn/Cd-RaZBP1 are present only in minute levels. Considering that RaZBP1 inhibited growth at low Zn, and despite the benefit that it provided to H. mesophaeum in the presence of high Zn and moderate Cd, these data indicate that the binding of excess Zn and Cd with RaZBP1 is not a trait that would be outright transmitted to H. mesophaeum.

A non-secreted plant defensin AtPDF2.6 conferred cadmium tolerance via its chelation in Arabidopsis.

Plant defensin AtPDF2.6 is not secreted to the apoplast and localized in cytoplasm. AtPDF2.6 is mainly expressed in root vascular bundles of xylem parenchyma cell, and significantly induced by Cd stress. AtPDF2.6 detoxicate cytoplasmic Cd via chelation, thus enhanced Cd tolerance in Arabidopsis. In order to detoxify the heavy metal cadmium (Cd), plants have evolved several mechanisms, among which chelation represents the major Cd-detoxification mechanism. In this study, we aimed to identify a new defensin protein involved in cytoplasmic Cd detoxification by using plant molecular genetics and physiological methods. The results of bioinformatic analysis showed that the Arabidopsis thaliana defensin gene AtPDF2.6 has a signal peptide that may mediate its secretion to the cell wall. Subcellular localization analysis revealed that AtPDF2.6 is localized to the cytoplasm and is not secreted to the apoplast, whereas histochemical analysis indicated that AtPDF2.6 is mainly expressed in the root xylem parenchyma cells and that its expression is significantly induced by Cd. An in vitro Cd-binding assay revealed that AtPDF2.6 has Cd-chelating activity. Heterologous overexpression of AtPDF2.6 increased Cd tolerance in Escherichia coli and yeast, and AtPDF2.6 overexpression significantly enhanced Cd tolerance in Arabidopsis, whereas functional disruption of AtPDF2.6 decreased Cd tolerance. These data suggest that AtPDF2.6 detoxifies cytoplasmic Cd via chelation and thereby enhances Cd tolerance in Arabidopsis. Our findings accordingly challenge the commonly accepted view of defensins as secreted proteins.

Revelation of microalgae's lipid production and resistance mechanism to ultra-high Cd stress by integrated transcriptome and physiochemical analyses

The ultra-high Cd polluted environment is a special habitat in nature. Analysis of the biological adaptation and resistance mechanism of Auxenochlorella protothecoides UTEX234 to ultra-high Cd stress would offer some inspiring understanding on Cd detoxification mechanism and help discovering highly active bioremediation agents. In this study, integrated analyses of the transcriptome, multi-physiological and biochemical data and fatty acid profilings of UTEX2341 were performed for the first time. It was found that exogenous Ca ions could alleviate Cd stress. Manganese-dependent superoxide dismutase and peroxidase also participated in intracellular detoxification. And non-enzymatic antioxidants rather than one specific enzymatic antioxidant were suggested to be used as “core antioxidants”, which witnessed better performance in Cd detoxification. In addition, Cd stress improved sixteen alkane value and biofuel yield and quality.

Reduction of cadmium toxicity in wheat through plasma technology

Cadmium (Cd) contamination in plant-derived food is a big concern. This study examines whether and how Ar/O2 and Ar/Air plasma techniques lead to Cd detoxification in wheat. Treatment with Ar/O2 and Ar/Air changed the seed surface and decreased the pH of seeds as well as the cultivation media. Generally, plants subjected to Cd treatment from seeds treated with Ar/O2and Ar/Air plasma showed considerable progress in morphology and total chlorophyll synthesis compared to Cd-treated wheat, suggesting that plasma technology is effective for Cd detoxification. Furthermore, Ar/O2 and Ar/Air plasma treated plants showed a significant decrease in root and shoot Cd concentration, which is consistent with the reduced expression of Cd transporters in the root (TaLCT1 and TaHMA2) compared with the plants not treated with plasma in response to Cd stress. This Cd inhibition is possibly accomplished by the decrease of pH reducing the bioavailability of Cd in the rhizosphere. These observations are in line with maintenance of total soluble protein along with reduced electrolyte leakage and cell death (%) in root and shoot due to Ar/O2 and Ar/Air treatments. Further, Cd-induced elevated H2O2 or oxidative damage in tissues was mainly diminished through the upregulation of antioxidant enzymes (SOD and CAT) and their corresponding genes (TaSOD and TaCAT) induced by Ar/O2 and Ar/Air plasma. Grafting results suggest that root originating nitric oxide signal possibly drives the mechanisms of Cd detoxification due to plasma treatment in wheat. These findings provide a novel and eco-friendly use of plasma technology for the mitigation of Cd toxicity in wheat plants.

Cadmium tolerance, distribution, and accumulation in Taraxacum ohwianum Kitam. as a potential Cd-hyperaccumulator

Strong capacity of cadmium tolerance and bioaccumulation in dandelions was revealed under 6-month treatments with different Cd concentrations (0, 50, 80, 100, 120 mg/kg). At the lowest Cd concentration (10 mg/kg), bioconcentration factors (BCF) of dandelions were greater than 1 and the transfer coefficient reached the maximum value of 0.74. At the Cd concentration of 100 mg/kg, the biomass of root was the greatest, the aboveground biomass reached 1.73 g/plant DW, which was 1.3 times of the blank control. Cd content in the leaves reached 100.11 mg/kg DW, which was in line with the threshold value (100 mg/kg) of Cd hyperaccumulators. In the roots of dandelions, Cd was mainly stored in the laticifer groups of phloem and cork, and xylem. The important Cd detoxification mechanism of plants included the combination of Cd and cork cell walls, the combination of Cd and xylem parenchyma cells, the combination of Cd and cell inclusions and precipitation in the laticifer. In leaves and scapes, Cd was preferentially accumulated in laticifer, epidermis cell, parenchyma cell inclusions and epidermis appendages, such as non-glandular hairs, etc., indicating that these sites played an important role in detoxification of heavy metals