Diurnal leaf and canopy gas exchanges of well-watered field grown cotton were measured. Our objective was to scale leaf-level values of transpiration and net assimilation to the whole canopy level using estimates of canopy leaf area. Single leaf gas exchange measurements were made with two portable photosynthesis systems and canopy measurements with four open Canopy Evapo-Transpiration and Assimilation (CETA) chamber systems. Canopy leaf area was measured at the end of the experiment and estimated during gas exchange by fitting values to a growth curve. Leaf level measurements were arithmetically scaled to estimate canopy level gas exchange based on canopy leaf area and then compared to the measured values. Scaled values of single leaf transpiration were very similar to canopy transpiration measurements, although both whole canopy transpiration and assimilation were overestimated around mid-day. We conclude that canopy cotton transpiration of well-watered field grown plants could be estimated within 5% throughout the day by scaling leaf level measurements to the whole canopy using measured canopy leaf area. Estimating canopy assimilation from leaf level measurements remains problematic.